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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 523 |
| AccI | GTMKAC | 2 cut(s) | 61, 325 |
| AclWI | GGATC | 1 cut(s) | 494 |
| AcoI | YGGCCR | 2 cut(s) | 9, 273 |
| AfaI | GTAC | 2 cut(s) | 111, 375 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 71, 223, 335, 513 |
| AflIII | ACRYGT | 1 cut(s) | 242 |
| AgsI | TTSAA | 1 cut(s) | 475 |
| AjnI | CCWGG | 4 cut(s) | 50, 85, 314, 349 |
| AlwI | GGATC | 1 cut(s) | 494 |
| AoxI | GGCC | 6 cut(s) | 9, 89, 207, 273, 353, 543 |
| ApeKI | GCWGC | 3 cut(s) | 45, 230, 309 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 453 |
| AspA2I | CCTAGG | 1 cut(s) | 512 |
| AspLEI | GCGC | 4 cut(s) | 71, 235, 335, 519 |
| AspS9I | GGNCC | 4 cut(s) | 89, 143, 353, 407 |
| AsuC2I | CCSGG | 4 cut(s) | 23, 126, 287, 390 |
| AsuHPI | GGTGA | 1 cut(s) | 509 |
| AvaII | GGWCC | 2 cut(s) | 143, 407 |
| AvrII | CCTAGG | 1 cut(s) | 512 |
| BbvI | GCAGC | 3 cut(s) | 57, 217, 321 |
| BccI | CCATC | 1 cut(s) | 436 |
| BceAI | ACGGC | 2 cut(s) | 93, 357 |
| BciT130I | CCWGG | 4 cut(s) | 52, 87, 316, 351 |
| BcnI | CCSGG | 4 cut(s) | 23, 126, 287, 390 |
| BfaI | CTAG | 2 cut(s) | 164, 513 |
| BfoI | RGCGCY | 2 cut(s) | 72, 336 |
| BfuAI | ACCTGC | 1 cut(s) | 523 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 77, 341, 529 |
| BisI | GCNGC | 5 cut(s) | 9, 46, 231, 273, 310 |
| BlnI | CCTAGG | 1 cut(s) | 512 |
| BlsI | GCNGC | 5 cut(s) | 10, 47, 232, 274, 311 |
| Bme1390I | CCNGG | 8 cut(s) | 23, 52, 87, 126, 287, 316, 351, 390 |
| Bme18I | GGWCC | 2 cut(s) | 143, 407 |
| BmgT120I | GGNCC | 4 cut(s) | 89, 143, 353, 407 |
| BmiI | GGNNCC | 2 cut(s) | 144, 408 |
| BmrFI | CCNGG | 8 cut(s) | 23, 52, 87, 126, 287, 316, 351, 390 |
| BpuMI | CCSGG | 4 cut(s) | 23, 126, 287, 390 |
| BsaAI | YACGTR | 2 cut(s) | 109, 373 |
| BsaJI | CCNNGG | 5 cut(s) | 86, 125, 350, 389, 512 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 71, 223, 335, 513 |
| Bse118I | RCCGGY | 4 cut(s) | 71, 91, 335, 355 |
| Bse3DI | GCAATG | 2 cut(s) | 87, 351 |
| BseBI | CCWGG | 4 cut(s) | 52, 87, 316, 351 |
| BseDI | CCNNGG | 5 cut(s) | 86, 125, 350, 389, 512 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 71, 223, 335, 513 |
| BseMI | GCAATG | 2 cut(s) | 87, 351 |
| BseMII | CTCAG | 1 cut(s) | 483 |
| BseRI | GAGGAG | 1 cut(s) | 203 |
| BseXI | GCAGC | 3 cut(s) | 57, 217, 321 |
| BseYI | CCCAGC | 2 cut(s) | 65, 329 |
| Bsh1285I | CGRYCG | 2 cut(s) | 19, 283 |
| BshFI | GGCC | 6 cut(s) | 11, 91, 209, 275, 355, 545 |
| BsiEI | CGRYCG | 2 cut(s) | 19, 283 |
| BsiSI | CCGG | 8 cut(s) | 23, 72, 92, 125, 287, 336, 356, 389 |
| BslFI | GGGAC | 2 cut(s) | 156, 420 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 71, 223, 335, 513 |
| BsmFI | GGGAC | 2 cut(s) | 156, 420 |
| BsnI | GGCC | 6 cut(s) | 11, 91, 209, 275, 355, 545 |
| Bsp143I | GATC | 1 cut(s) | 499 |
| BspANI | GGCC | 6 cut(s) | 11, 91, 209, 275, 355, 545 |
| BspCNI | CTCAG | 1 cut(s) | 484 |
| BspHI | TCATGA | 1 cut(s) | 237 |
| BspLI | GGNNCC | 2 cut(s) | 144, 408 |
| BspMI | ACCTGC | 1 cut(s) | 523 |
| BspPI | GGATC | 1 cut(s) | 494 |
| BsrDI | GCAATG | 2 cut(s) | 87, 351 |
| BsrFI | RCCGGY | 4 cut(s) | 71, 91, 335, 355 |
| BssAI | RCCGGY | 4 cut(s) | 71, 91, 335, 355 |
| BssECI | CCNNGG | 5 cut(s) | 86, 125, 350, 389, 512 |
| BssMI | GATC | 1 cut(s) | 499 |
| BssT1I | CCWWGG | 1 cut(s) | 512 |
| Bst2UI | CCWGG | 4 cut(s) | 52, 87, 316, 351 |
| BstBAI | YACGTR | 2 cut(s) | 109, 373 |
| BstC8I | GCNNGC | 6 cut(s) | 35, 73, 299, 337, 462, 521 |
| BstDEI | CTNAG | 1 cut(s) | 492 |
| BstH2I | RGCGCY | 2 cut(s) | 72, 336 |
| BstHHI | GCGC | 4 cut(s) | 71, 235, 335, 519 |
| BstKTI | GATC | 1 cut(s) | 502 |
| BstMBI | GATC | 1 cut(s) | 499 |
| BstMCI | CGRYCG | 2 cut(s) | 19, 283 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 77, 112, 341, 376, 529 |
| BstNI | CCWGG | 4 cut(s) | 52, 87, 316, 351 |
| BstSCI | CCNGG | 8 cut(s) | 21, 50, 85, 124, 285, 314, 349, 388 |
| BstV1I | GCAGC | 3 cut(s) | 57, 217, 321 |
| BsuRI | GGCC | 6 cut(s) | 11, 91, 209, 275, 355, 545 |
| BveI | ACCTGC | 1 cut(s) | 523 |
| Cac8I | GCNNGC | 6 cut(s) | 35, 73, 299, 337, 462, 521 |
| CciI | TCATGA | 1 cut(s) | 237 |
| CfoI | GCGC | 4 cut(s) | 71, 235, 335, 519 |
| Cfr10I | RCCGGY | 4 cut(s) | 71, 91, 335, 355 |
| Cfr13I | GGNCC | 4 cut(s) | 89, 143, 353, 407 |
| Csp6I | GTAC | 2 cut(s) | 110, 374 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 111, 146, 375, 410 |
| CviAII | CATG | 1 cut(s) | 238 |
| CviJI | RGCY | 9 cut(s) | 11, 91, 209, 215, 230, 275, 355, 523, 545 |
| CviKI_1 | RGCY | 9 cut(s) | 11, 91, 209, 215, 230, 275, 355, 523, 545 |
| CviQI | GTAC | 2 cut(s) | 110, 374 |
| DdeI | CTNAG | 1 cut(s) | 492 |
| DpnI | GATC | 1 cut(s) | 501 |
| DpnII | GATC | 1 cut(s) | 499 |
| EaeI | YGGCCR | 2 cut(s) | 9, 273 |
| EciI | GGCGGA | 2 cut(s) | 461, 492 |
| Eco130I | CCWWGG | 1 cut(s) | 512 |
| Eco47I | GGWCC | 2 cut(s) | 143, 407 |
| EcoRII | CCWGG | 4 cut(s) | 50, 85, 314, 349 |
| EcoT14I | CCWWGG | 1 cut(s) | 512 |
| ErhI | CCWWGG | 1 cut(s) | 512 |
| FaeI | CATG | 1 cut(s) | 241 |
| FaiI | YATR | 6 cut(s) | 98, 155, 239, 362, 419, 509 |
| FaqI | GGGAC | 2 cut(s) | 156, 420 |
| FatI | CATG | 1 cut(s) | 237 |
| FblI | GTMKAC | 2 cut(s) | 61, 325 |
| Fnu4HI | GCNGC | 5 cut(s) | 9, 46, 231, 273, 310 |
| Fsp4HI | GCNGC | 5 cut(s) | 9, 46, 231, 273, 310 |
| FspBI | CTAG | 2 cut(s) | 164, 513 |
| GlaI | GCGC | 4 cut(s) | 70, 234, 334, 518 |
| GluI | GCNGC | 5 cut(s) | 9, 46, 231, 273, 310 |
| GsaI | CCCAGC | 2 cut(s) | 69, 333 |
| HaeII | RGCGCY | 2 cut(s) | 72, 336 |
| HaeIII | GGCC | 6 cut(s) | 11, 91, 209, 275, 355, 545 |
| HapII | CCGG | 8 cut(s) | 23, 72, 92, 125, 287, 336, 356, 389 |
| HhaI | GCGC | 4 cut(s) | 71, 235, 335, 519 |
| Hin1II | CATG | 1 cut(s) | 241 |
| Hin6I | GCGC | 4 cut(s) | 69, 233, 333, 517 |
| HinP1I | GCGC | 4 cut(s) | 69, 233, 333, 517 |
| HincII | GTYRAC | 2 cut(s) | 62, 326 |
| HindII | GTYRAC | 2 cut(s) | 62, 326 |
| HinfI | GANTC | 2 cut(s) | 449, 548 |
| HpaII | CCGG | 8 cut(s) | 23, 72, 92, 125, 287, 336, 356, 389 |
| HphI | GGTGA | 1 cut(s) | 509 |
| Hpy166II | GTNNAC | 2 cut(s) | 62, 326 |
| Hpy188III | TCNNGA | 4 cut(s) | 135, 238, 399, 472 |
| Hpy8I | GTNNAC | 2 cut(s) | 62, 326 |
| HpyAV | CCTTC | 2 cut(s) | 199, 464 |
| HpyCH4IV | ACGT | 4 cut(s) | 108, 196, 244, 372 |
| HpyCH4V | TGCA | 2 cut(s) | 106, 370 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 77, 112, 341, 376, 529 |
| HpyF3I | CTNAG | 1 cut(s) | 492 |
| HpySE526I | ACGT | 4 cut(s) | 108, 196, 244, 372 |
| Hsp92II | CATG | 1 cut(s) | 241 |
| HspAI | GCGC | 4 cut(s) | 69, 233, 333, 517 |
| KroI | GCCGGC | 2 cut(s) | 71, 335 |
| KroNI | GCCGGC | 2 cut(s) | 73, 337 |
| Kzo9I | GATC | 1 cut(s) | 499 |
| LmnI | GCTCC | 1 cut(s) | 181 |
| Lsp1109I | GCAGC | 3 cut(s) | 57, 217, 321 |
| MaeI | CTAG | 2 cut(s) | 164, 513 |
| MaeII | ACGT | 4 cut(s) | 108, 196, 244, 372 |
| MaeIII | GTNAC | 2 cut(s) | 39, 303 |
| MalI | GATC | 1 cut(s) | 501 |
| MboI | GATC | 1 cut(s) | 499 |
| MnlI | CCTC | 4 cut(s) | 181, 184, 244, 487 |
| MroNI | GCCGGC | 2 cut(s) | 71, 335 |
| MroXI | GAANNNNTTC | 1 cut(s) | 453 |
| MslI | CAYNNNNRTG | 4 cut(s) | 80, 101, 344, 365 |
| MspA1I | CMGCKG | 2 cut(s) | 115, 379 |
| MspI | CCGG | 8 cut(s) | 23, 72, 92, 125, 287, 336, 356, 389 |
| MspR9I | CCNGG | 8 cut(s) | 23, 52, 87, 126, 287, 316, 351, 390 |
| MvaI | CCWGG | 4 cut(s) | 52, 87, 316, 351 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 77, 112, 341, 376, 529 |
| NaeI | GCCGGC | 2 cut(s) | 73, 337 |
| NciI | CCSGG | 4 cut(s) | 23, 126, 287, 390 |
| NdeII | GATC | 1 cut(s) | 499 |
| NgoMIV | GCCGGC | 2 cut(s) | 71, 335 |
| NlaIII | CATG | 1 cut(s) | 241 |
| NlaIV | GGNNCC | 2 cut(s) | 144, 408 |
| NmeAIII | GCCGAG | 1 cut(s) | 508 |
| NmuCI | GTSAC | 2 cut(s) | 39, 303 |
| PagI | TCATGA | 1 cut(s) | 237 |
| PdiI | GCCGGC | 2 cut(s) | 73, 337 |
| PdmI | GAANNNNTTC | 1 cut(s) | 453 |
| PfeI | GAWTC | 2 cut(s) | 449, 548 |
| PkrI | GCNGC | 5 cut(s) | 10, 47, 232, 274, 311 |
| Ppu21I | YACGTR | 2 cut(s) | 109, 373 |
| Psp6I | CCWGG | 4 cut(s) | 50, 85, 314, 349 |
| PspFI | CCCAGC | 2 cut(s) | 65, 329 |
| PspGI | CCWGG | 4 cut(s) | 50, 85, 314, 349 |
| PspN4I | GGNNCC | 2 cut(s) | 144, 408 |
| PspPI | GGNCC | 4 cut(s) | 89, 143, 353, 407 |
| RsaI | GTAC | 2 cut(s) | 111, 375 |
| RsaNI | GTAC | 2 cut(s) | 110, 374 |
| RseI | CAYNNNNRTG | 4 cut(s) | 80, 101, 344, 365 |
| SalI | GTCGAC | 2 cut(s) | 60, 324 |
| SatI | GCNGC | 5 cut(s) | 9, 46, 231, 273, 310 |
| Sau3AI | GATC | 1 cut(s) | 499 |
| Sau96I | GGNCC | 4 cut(s) | 89, 143, 353, 407 |
| ScrFI | CCNGG | 8 cut(s) | 23, 52, 87, 126, 287, 316, 351, 390 |
| SetI | ASST | 7 cut(s) | 111, 199, 228, 247, 375, 514, 537 |
| SinI | GGWCC | 2 cut(s) | 143, 407 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 80, 101, 344, 365 |
| SspMI | CTAG | 2 cut(s) | 164, 513 |
| StyD4I | CCNGG | 8 cut(s) | 21, 50, 85, 124, 285, 314, 349, 388 |
| StyI | CCWWGG | 1 cut(s) | 512 |
| TaiI | ACGT | 4 cut(s) | 111, 199, 247, 375 |
| TaqI | TCGA | 2 cut(s) | 61, 325 |
| TauI | GCSGC | 2 cut(s) | 11, 275 |
| TfiI | GAWTC | 2 cut(s) | 449, 548 |
| TseFI | GTSAC | 2 cut(s) | 39, 303 |
| TseI | GCWGC | 3 cut(s) | 45, 230, 309 |
| Tsp45I | GTSAC | 2 cut(s) | 39, 303 |
| VpaK11BI | GGWCC | 2 cut(s) | 143, 407 |
| XmaJI | CCTAGG | 1 cut(s) | 512 |
| XmiI | GTMKAC | 2 cut(s) | 61, 325 |
| XmnI | GAANNNNTTC | 1 cut(s) | 453 |
| XspI | CTAG | 2 cut(s) | 164, 513 |