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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 394, 547 |
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 3 cut(s) | 379, 406, 659 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 3 cut(s) | 90, 277, 479 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 2 cut(s) | 311, 546 |
| AcyI | GRCGYC | 1 cut(s) | 660 |
| AfaI | GTAC | 4 cut(s) | 176, 373, 381, 408 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 77, 368, 508, 610, 702 |
| AjnI | CCWGG | 3 cut(s) | 151, 347, 700 |
| AluBI | AGCT | 2 cut(s) | 181, 729 |
| AluI | AGCT | 2 cut(s) | 181, 729 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 6 cut(s) | 108, 154, 246, 386, 576, 704 |
| ApaI | GGGCCC | 1 cut(s) | 708 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 716 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 5 cut(s) | 92, 325, 544, 662, 670 |
| AspS9I | GGNCC | 7 cut(s) | 247, 386, 415, 440, 559, 704, 705 |
| AsuC2I | CCSGG | 4 cut(s) | 107, 117, 363, 384 |
| AsuHPI | GGTGA | 1 cut(s) | 703 |
| AsuNHI | GCTAGC | 1 cut(s) | 181 |
| AvaII | GGWCC | 3 cut(s) | 415, 440, 559 |
| BaeGI | GKGCMC | 1 cut(s) | 708 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 363, 363, 396, 396 |
| BanI | GGYRCC | 3 cut(s) | 379, 406, 659 |
| BanII | GRGCYC | 1 cut(s) | 708 |
| BbvCI | CCTCAGC | 1 cut(s) | 243 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 703 |
| BccI | CCATC | 2 cut(s) | 59, 430 |
| BciT130I | CCWGG | 3 cut(s) | 153, 349, 702 |
| BcnI | CCSGG | 4 cut(s) | 107, 117, 363, 384 |
| BfaI | CTAG | 2 cut(s) | 182, 513 |
| BfoI | RGCGCY | 2 cut(s) | 663, 671 |
| BfuAI | ACCTGC | 2 cut(s) | 394, 547 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 5 cut(s) | 139, 142, 145, 491, 717 |
| BlsI | GCNGC | 5 cut(s) | 140, 143, 146, 492, 718 |
| Bme1390I | CCNGG | 7 cut(s) | 107, 117, 153, 349, 363, 384, 702 |
| Bme18I | GGWCC | 3 cut(s) | 415, 440, 559 |
| BmgT120I | GGNCC | 7 cut(s) | 247, 386, 415, 440, 559, 704, 705 |
| BmiI | GGNNCC | 7 cut(s) | 381, 387, 408, 417, 441, 661, 706 |
| BmrFI | CCNGG | 7 cut(s) | 107, 117, 153, 349, 363, 384, 702 |
| BmsI | GCATC | 3 cut(s) | 390, 583, 609 |
| BmtI | GCTAGC | 1 cut(s) | 185 |
| BpmI | CTGGAG | 1 cut(s) | 650 |
| Bpu10I | CCTNAGC | 1 cut(s) | 243 |
| BpuMI | CCSGG | 4 cut(s) | 107, 117, 363, 384 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaHI | GRCGYC | 1 cut(s) | 660 |
| BsaJI | CCNNGG | 5 cut(s) | 105, 115, 383, 545, 701 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 77, 368, 508, 610, 702 |
| Bse1I | ACTGG | 4 cut(s) | 192, 373, 389, 633 |
| BseBI | CCWGG | 3 cut(s) | 153, 349, 702 |
| BseDI | CCNNGG | 5 cut(s) | 105, 115, 383, 545, 701 |
| BseGI | GGATG | 3 cut(s) | 51, 441, 624 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 77, 368, 508, 610, 702 |
| BseMII | CTCAG | 3 cut(s) | 234, 414, 689 |
| BseNI | ACTGG | 4 cut(s) | 192, 373, 389, 633 |
| BseRI | GAGGAG | 1 cut(s) | 236 |
| BseSI | GKGCMC | 1 cut(s) | 708 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 703 |
| BseYI | CCCAGC | 2 cut(s) | 70, 664 |
| Bsh1236I | CGCG | 3 cut(s) | 90, 277, 479 |
| Bsh1285I | CGRYCG | 1 cut(s) | 312 |
| BshFI | GGCC | 6 cut(s) | 110, 156, 248, 388, 578, 706 |
| BshNI | GGYRCC | 3 cut(s) | 379, 406, 659 |
| BsiEI | CGRYCG | 1 cut(s) | 312 |
| BsiSI | CCGG | 4 cut(s) | 107, 117, 363, 383 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 77, 368, 508, 610, 702 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsnI | GGCC | 6 cut(s) | 110, 156, 248, 388, 578, 706 |
| Bsp120I | GGGCCC | 1 cut(s) | 704 |
| Bsp1286I | GDGCHC | 1 cut(s) | 708 |
| Bsp1407I | TGTACA | 1 cut(s) | 174 |
| Bsp143I | GATC | 3 cut(s) | 160, 193, 642 |
| BspANI | GGCC | 6 cut(s) | 110, 156, 248, 388, 578, 706 |
| BspCNI | CTCAG | 3 cut(s) | 235, 415, 688 |
| BspFNI | CGCG | 3 cut(s) | 90, 277, 479 |
| BspLI | GGNNCC | 7 cut(s) | 381, 387, 408, 417, 441, 661, 706 |
| BspMI | ACCTGC | 2 cut(s) | 394, 547 |
| BspOI | GCTAGC | 1 cut(s) | 185 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BspT107I | GGYRCC | 3 cut(s) | 379, 406, 659 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrGI | TGTACA | 1 cut(s) | 174 |
| BsrI | ACTGG | 4 cut(s) | 192, 373, 389, 633 |
| BssECI | CCNNGG | 5 cut(s) | 105, 115, 383, 545, 701 |
| BssMI | GATC | 3 cut(s) | 160, 193, 642 |
| BssNI | GRCGYC | 1 cut(s) | 660 |
| Bst2UI | CCWGG | 3 cut(s) | 153, 349, 702 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 258, 336 |
| Bst6I | CTCTTC | 1 cut(s) | 287 |
| BstACI | GRCGYC | 1 cut(s) | 660 |
| BstAUI | TGTACA | 1 cut(s) | 174 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 149, 183, 540, 727 |
| BstDEI | CTNAG | 3 cut(s) | 243, 423, 675 |
| BstF5I | GGATG | 3 cut(s) | 51, 441, 624 |
| BstFNI | CGCG | 3 cut(s) | 90, 277, 479 |
| BstH2I | RGCGCY | 2 cut(s) | 663, 671 |
| BstHHI | GCGC | 5 cut(s) | 92, 325, 544, 662, 670 |
| BstKTI | GATC | 3 cut(s) | 163, 196, 645 |
| BstMBI | GATC | 3 cut(s) | 160, 193, 642 |
| BstMCI | CGRYCG | 1 cut(s) | 312 |
| BstNI | CCWGG | 3 cut(s) | 153, 349, 702 |
| BstSCI | CCNGG | 7 cut(s) | 105, 115, 151, 347, 361, 382, 700 |
| BstSLI | GKGCMC | 1 cut(s) | 708 |
| BstUI | CGCG | 3 cut(s) | 90, 277, 479 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 703 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 6 cut(s) | 110, 156, 248, 388, 578, 706 |
| BtsCI | GGATG | 3 cut(s) | 51, 441, 624 |
| BtsI | GCAGTG | 1 cut(s) | 485 |
| BtsIMutI | CAGTG | 3 cut(s) | 185, 366, 485 |
| BveI | ACCTGC | 2 cut(s) | 394, 547 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 149, 183, 540, 727 |
| CfoI | GCGC | 5 cut(s) | 92, 325, 544, 662, 670 |
| Cfr13I | GGNCC | 7 cut(s) | 247, 386, 415, 440, 559, 704, 705 |
| CseI | GACGC | 2 cut(s) | 307, 485 |
| Csp6I | GTAC | 4 cut(s) | 175, 372, 380, 407 |
| CviQI | GTAC | 4 cut(s) | 175, 372, 380, 407 |
| DdeI | CTNAG | 3 cut(s) | 243, 423, 675 |
| DinI | GGCGCC | 1 cut(s) | 661 |
| DpnI | GATC | 3 cut(s) | 162, 195, 644 |
| DpnII | GATC | 3 cut(s) | 160, 193, 642 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 1 cut(s) | 287 |
| EarI | CTCTTC | 1 cut(s) | 287 |
| EciI | GGCGGA | 4 cut(s) | 37, 206, 525, 665 |
| Eco24I | GRGCYC | 1 cut(s) | 708 |
| Eco47I | GGWCC | 3 cut(s) | 415, 440, 559 |
| Eco57I | CTGAAG | 2 cut(s) | 311, 546 |
| EcoO109I | RGGNCCY | 1 cut(s) | 705 |
| EcoRII | CCWGG | 3 cut(s) | 151, 347, 700 |
| EcoT38I | GRGCYC | 1 cut(s) | 708 |
| EgeI | GGCGCC | 1 cut(s) | 661 |
| EheI | GGCGCC | 1 cut(s) | 661 |
| FaiI | YATR | 4 cut(s) | 173, 201, 395, 745 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FauI | CCCGC | 2 cut(s) | 8, 156 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 5 cut(s) | 139, 142, 145, 491, 717 |
| FokI | GGATG | 3 cut(s) | 38, 448, 631 |
| FriOI | GRGCYC | 1 cut(s) | 708 |
| Fsp4HI | GCNGC | 5 cut(s) | 139, 142, 145, 491, 717 |
| FspBI | CTAG | 2 cut(s) | 182, 513 |
| GlaI | GCGC | 5 cut(s) | 91, 324, 543, 661, 669 |
| GluI | GCNGC | 5 cut(s) | 139, 142, 145, 491, 717 |
| GsaI | CCCAGC | 2 cut(s) | 74, 668 |
| GsuI | CTGGAG | 1 cut(s) | 650 |
| HaeII | RGCGCY | 2 cut(s) | 663, 671 |
| HaeIII | GGCC | 6 cut(s) | 110, 156, 248, 388, 578, 706 |
| HapII | CCGG | 4 cut(s) | 107, 117, 363, 383 |
| HgaI | GACGC | 2 cut(s) | 307, 485 |
| HhaI | GCGC | 5 cut(s) | 92, 325, 544, 662, 670 |
| Hin1I | GRCGYC | 1 cut(s) | 660 |
| Hin6I | GCGC | 5 cut(s) | 90, 323, 542, 660, 668 |
| HinP1I | GCGC | 5 cut(s) | 90, 323, 542, 660, 668 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 3 cut(s) | 482, 653, 679 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 4 cut(s) | 107, 117, 363, 383 |
| HphI | GGTGA | 1 cut(s) | 703 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 5 cut(s) | 67, 508, 678, 735, 785 |
| Hpy188III | TCNNGA | 1 cut(s) | 696 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy99I | CGWCG | 1 cut(s) | 593 |
| HpyAV | CCTTC | 4 cut(s) | 232, 460, 521, 718 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 258, 336 |
| HpyCH4IV | ACGT | 2 cut(s) | 217, 340 |
| HpyCH4V | TGCA | 5 cut(s) | 403, 596, 622, 725, 747 |
| HpyF3I | CTNAG | 3 cut(s) | 243, 423, 675 |
| HpySE526I | ACGT | 2 cut(s) | 217, 340 |
| Hsp92I | GRCGYC | 1 cut(s) | 660 |
| HspAI | GCGC | 5 cut(s) | 90, 323, 542, 660, 668 |
| KasI | GGCGCC | 1 cut(s) | 659 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 160, 193, 642 |
| LmnI | GCTCC | 1 cut(s) | 631 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 703 |
| LweI | GCATC | 3 cut(s) | 390, 583, 609 |
| MaeI | CTAG | 2 cut(s) | 182, 513 |
| MaeII | ACGT | 2 cut(s) | 217, 340 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 3 cut(s) | 162, 195, 644 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboI | GATC | 3 cut(s) | 160, 193, 642 |
| MboII | GAAGA | 2 cut(s) | 188, 304 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 708 |
| Mly113I | GGCGCC | 1 cut(s) | 660 |
| MlyI | GAGTC | 1 cut(s) | 662 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 2 cut(s) | 170, 398 |
| MspA1I | CMGCKG | 4 cut(s) | 74, 412, 565, 716 |
| MspI | CCGG | 4 cut(s) | 107, 117, 363, 383 |
| MspR9I | CCNGG | 7 cut(s) | 107, 117, 153, 349, 363, 384, 702 |
| MvaI | CCWGG | 3 cut(s) | 153, 349, 702 |
| MvnI | CGCG | 3 cut(s) | 90, 277, 479 |
| NarI | GGCGCC | 1 cut(s) | 660 |
| NciI | CCSGG | 4 cut(s) | 107, 117, 363, 384 |
| NdeII | GATC | 3 cut(s) | 160, 193, 642 |
| NheI | GCTAGC | 1 cut(s) | 181 |
| NlaIV | GGNNCC | 7 cut(s) | 381, 387, 408, 417, 441, 661, 706 |
| NmeAIII | GCCGAG | 1 cut(s) | 570 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 305 |
| PfeI | GAWTC | 2 cut(s) | 482, 679 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 5 cut(s) | 140, 143, 146, 492, 718 |
| PleI | GAGTC | 1 cut(s) | 661 |
| PluTI | GGCGCC | 1 cut(s) | 663 |
| PpsI | GAGTC | 1 cut(s) | 661 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| Psp6I | CCWGG | 3 cut(s) | 151, 347, 700 |
| PspFI | CCCAGC | 2 cut(s) | 70, 664 |
| PspGI | CCWGG | 3 cut(s) | 151, 347, 700 |
| PspN4I | GGNNCC | 7 cut(s) | 381, 387, 408, 417, 441, 661, 706 |
| PspOMI | GGGCCC | 1 cut(s) | 704 |
| PspPI | GGNCC | 7 cut(s) | 247, 386, 415, 440, 559, 704, 705 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 4 cut(s) | 176, 373, 381, 408 |
| RsaNI | GTAC | 4 cut(s) | 175, 372, 380, 407 |
| RseI | CAYNNNNRTG | 2 cut(s) | 170, 398 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 5 cut(s) | 139, 142, 145, 491, 717 |
| Sau3AI | GATC | 3 cut(s) | 160, 193, 642 |
| Sau96I | GGNCC | 7 cut(s) | 247, 386, 415, 440, 559, 704, 705 |
| SchI | GAGTC | 1 cut(s) | 662 |
| ScrFI | CCNGG | 7 cut(s) | 107, 117, 153, 349, 363, 384, 702 |
| SduI | GDGCHC | 1 cut(s) | 708 |
| SetI | ASST | 9 cut(s) | 183, 220, 343, 408, 424, 561, 641, 703, 731 |
| SfaNI | GCATC | 3 cut(s) | 390, 583, 609 |
| SfoI | GGCGCC | 1 cut(s) | 661 |
| SinI | GGWCC | 3 cut(s) | 415, 440, 559 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 170, 398 |
| SspDI | GGCGCC | 1 cut(s) | 659 |
| SspI | AATATT | 1 cut(s) | 571 |
| SspMI | CTAG | 2 cut(s) | 182, 513 |
| StyD4I | CCNGG | 7 cut(s) | 105, 115, 151, 347, 361, 382, 700 |
| TaaI | ACNGT | 3 cut(s) | 11, 258, 336 |
| TaiI | ACGT | 2 cut(s) | 220, 343 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TatI | WGTACW | 1 cut(s) | 174 |
| TauI | GCSGC | 2 cut(s) | 147, 493 |
| TfiI | GAWTC | 2 cut(s) | 482, 679 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 3 cut(s) | 192, 373, 492 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 716 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspDTI | ATGAA | 2 cut(s) | 188, 300 |
| TspRI | CASTG | 3 cut(s) | 192, 373, 492 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 3 cut(s) | 415, 440, 559 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 607 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XspI | CTAG | 2 cut(s) | 182, 513 |