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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 496 |
| Acc65I | GGTACC | 1 cut(s) | 95 |
| AccB1I | GGYRCC | 2 cut(s) | 95, 370 |
| AccBSI | CCGCTC | 2 cut(s) | 219, 270 |
| AccII | CGCG | 3 cut(s) | 56, 304, 395 |
| AcoI | YGGCCR | 1 cut(s) | 310 |
| AfaI | GTAC | 1 cut(s) | 97 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 84, 143, 259, 262, 263, 292, 405 |
| AjnI | CCWGG | 1 cut(s) | 297 |
| Alw21I | GWGCWC | 1 cut(s) | 63 |
| Ama87I | CYCGRG | 1 cut(s) | 169 |
| AoxI | GGCC | 5 cut(s) | 152, 272, 295, 310, 402 |
| ApaI | GGGCCC | 2 cut(s) | 156, 276 |
| ApeKI | GCWGC | 1 cut(s) | 124 |
| Asp718I | GGTACC | 1 cut(s) | 95 |
| AspLEI | GCGC | 2 cut(s) | 41, 304 |
| AspS9I | GGNCC | 7 cut(s) | 102, 152, 153, 272, 273, 295, 408 |
| AsuC2I | CCSGG | 5 cut(s) | 36, 100, 324, 368, 406 |
| AsuHPI | GGTGA | 1 cut(s) | 499 |
| AvaI | CYCGRG | 1 cut(s) | 169 |
| AvaII | GGWCC | 2 cut(s) | 102, 408 |
| BaeGI | GKGCMC | 2 cut(s) | 156, 276 |
| BanI | GGYRCC | 2 cut(s) | 95, 370 |
| BanII | GRGCYC | 2 cut(s) | 156, 276 |
| Bbv12I | GWGCWC | 1 cut(s) | 63 |
| BbvI | GCAGC | 1 cut(s) | 111 |
| BccI | CCATC | 2 cut(s) | 143, 221 |
| BceAI | ACGGC | 1 cut(s) | 493 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 160, 194 |
| BciT130I | CCWGG | 1 cut(s) | 299 |
| BcnI | CCSGG | 5 cut(s) | 36, 100, 324, 368, 406 |
| BfaI | CTAG | 3 cut(s) | 228, 234, 342 |
| BglII | AGATCT | 1 cut(s) | 424 |
| BisI | GCNGC | 5 cut(s) | 16, 125, 261, 313, 481 |
| BlsI | GCNGC | 5 cut(s) | 17, 126, 262, 314, 482 |
| Bme1390I | CCNGG | 6 cut(s) | 36, 100, 299, 324, 368, 406 |
| Bme18I | GGWCC | 2 cut(s) | 102, 408 |
| BmeT110I | CYCGRG | 1 cut(s) | 169 |
| BmgT120I | GGNCC | 7 cut(s) | 102, 152, 153, 272, 273, 295, 408 |
| BmiI | GGNNCC | 6 cut(s) | 97, 154, 274, 284, 372, 409 |
| BmrFI | CCNGG | 6 cut(s) | 36, 100, 299, 324, 368, 406 |
| BmsI | GCATC | 1 cut(s) | 322 |
| BpuMI | CCSGG | 5 cut(s) | 36, 100, 324, 368, 406 |
| BsaJI | CCNNGG | 4 cut(s) | 170, 286, 366, 399 |
| BsaWI | WCCGGW | 2 cut(s) | 63, 358 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 84, 143, 259, 262, 263, 292, 405 |
| Bse118I | RCCGGY | 1 cut(s) | 442 |
| BseBI | CCWGG | 1 cut(s) | 299 |
| BseDI | CCNNGG | 4 cut(s) | 170, 286, 366, 399 |
| BseGI | GGATG | 4 cut(s) | 154, 213, 337, 436 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 84, 143, 259, 262, 263, 292, 405 |
| BseSI | GKGCMC | 2 cut(s) | 156, 276 |
| BseX3I | CGGCCG | 1 cut(s) | 310 |
| BseXI | GCAGC | 1 cut(s) | 111 |
| Bsh1236I | CGCG | 3 cut(s) | 56, 304, 395 |
| Bsh1285I | CGRYCG | 1 cut(s) | 313 |
| BshFI | GGCC | 5 cut(s) | 154, 274, 297, 312, 404 |
| BshNI | GGYRCC | 2 cut(s) | 95, 370 |
| BsiEI | CGRYCG | 1 cut(s) | 313 |
| BsiHKAI | GWGCWC | 1 cut(s) | 63 |
| BsiHKCI | CYCGRG | 1 cut(s) | 169 |
| BsiSI | CCGG | 8 cut(s) | 36, 64, 100, 324, 359, 368, 405, 443 |
| BslFI | GGGAC | 2 cut(s) | 349, 421 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 84, 143, 259, 262, 263, 292, 405 |
| BsmFI | GGGAC | 2 cut(s) | 349, 421 |
| BsnI | GGCC | 5 cut(s) | 154, 274, 297, 312, 404 |
| BsoBI | CYCGRG | 1 cut(s) | 169 |
| Bsp120I | GGGCCC | 2 cut(s) | 152, 272 |
| Bsp1286I | GDGCHC | 3 cut(s) | 63, 156, 276 |
| Bsp143I | GATC | 1 cut(s) | 424 |
| BspANI | GGCC | 5 cut(s) | 154, 274, 297, 312, 404 |
| BspFNI | CGCG | 3 cut(s) | 56, 304, 395 |
| BspLI | GGNNCC | 6 cut(s) | 97, 154, 274, 284, 372, 409 |
| BspT107I | GGYRCC | 2 cut(s) | 95, 370 |
| BsrBI | CCGCTC | 2 cut(s) | 219, 270 |
| BsrFI | RCCGGY | 1 cut(s) | 442 |
| BssAI | RCCGGY | 1 cut(s) | 442 |
| BssECI | CCNNGG | 4 cut(s) | 170, 286, 366, 399 |
| BssMI | GATC | 1 cut(s) | 424 |
| Bst2UI | CCWGG | 1 cut(s) | 299 |
| Bst4CI | ACNGT | 2 cut(s) | 72, 121 |
| Bst6I | CTCTTC | 1 cut(s) | 395 |
| BstC8I | GCNNGC | 3 cut(s) | 48, 272, 444 |
| BstEII | GGTNACC | 1 cut(s) | 487 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 290 |
| BstF5I | GGATG | 4 cut(s) | 154, 213, 337, 436 |
| BstFNI | CGCG | 3 cut(s) | 56, 304, 395 |
| BstHHI | GCGC | 2 cut(s) | 41, 304 |
| BstKTI | GATC | 1 cut(s) | 427 |
| BstMBI | GATC | 1 cut(s) | 424 |
| BstMCI | CGRYCG | 1 cut(s) | 313 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 12, 21, 47, 133, 230, 401 |
| BstNI | CCWGG | 1 cut(s) | 299 |
| BstNSI | RCATGY | 1 cut(s) | 435 |
| BstPI | GGTNACC | 1 cut(s) | 487 |
| BstSCI | CCNGG | 6 cut(s) | 34, 98, 297, 322, 366, 404 |
| BstSLI | GKGCMC | 2 cut(s) | 156, 276 |
| BstUI | CGCG | 3 cut(s) | 56, 304, 395 |
| BstV1I | GCAGC | 1 cut(s) | 111 |
| BstX2I | RGATCY | 1 cut(s) | 424 |
| BstYI | RGATCY | 1 cut(s) | 424 |
| BstZI | CGGCCG | 1 cut(s) | 310 |
| BsuRI | GGCC | 5 cut(s) | 154, 274, 297, 312, 404 |
| BtsCI | GGATG | 4 cut(s) | 154, 213, 337, 436 |
| Cac8I | GCNNGC | 3 cut(s) | 48, 272, 444 |
| CfoI | GCGC | 2 cut(s) | 41, 304 |
| Cfr10I | RCCGGY | 1 cut(s) | 442 |
| Cfr13I | GGNCC | 7 cut(s) | 102, 152, 153, 272, 273, 295, 408 |
| CseI | GACGC | 1 cut(s) | 384 |
| Csp6I | GTAC | 1 cut(s) | 96 |
| CviAII | CATG | 2 cut(s) | 432, 484 |
| CviJI | RGCY | 9 cut(s) | 154, 175, 233, 274, 285, 297, 312, 353, 404 |
| CviKI_1 | RGCY | 9 cut(s) | 154, 175, 233, 274, 285, 297, 312, 353, 404 |
| CviQI | GTAC | 1 cut(s) | 96 |
| DpnI | GATC | 1 cut(s) | 426 |
| DpnII | GATC | 1 cut(s) | 424 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 496 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 496 |
| EaeI | YGGCCR | 1 cut(s) | 310 |
| EagI | CGGCCG | 1 cut(s) | 310 |
| Eam1104I | CTCTTC | 1 cut(s) | 395 |
| EarI | CTCTTC | 1 cut(s) | 395 |
| EciI | GGCGGA | 4 cut(s) | 33, 103, 209, 429 |
| EclXI | CGGCCG | 1 cut(s) | 310 |
| Eco24I | GRGCYC | 2 cut(s) | 156, 276 |
| Eco47I | GGWCC | 2 cut(s) | 102, 408 |
| Eco52I | CGGCCG | 1 cut(s) | 310 |
| Eco88I | CYCGRG | 1 cut(s) | 169 |
| Eco91I | GGTNACC | 1 cut(s) | 487 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 290 |
| EcoO109I | RGGNCCY | 1 cut(s) | 295 |
| EcoO65I | GGTNACC | 1 cut(s) | 487 |
| EcoRII | CCWGG | 1 cut(s) | 297 |
| EcoT38I | GRGCYC | 2 cut(s) | 156, 276 |
| FaeI | CATG | 2 cut(s) | 435, 487 |
| FaiI | YATR | 7 cut(s) | 113, 210, 433, 450, 485, 503, 505 |
| FaqI | GGGAC | 2 cut(s) | 349, 421 |
| FatI | CATG | 2 cut(s) | 431, 483 |
| FauI | CCCGC | 4 cut(s) | 39, 141, 256, 263 |
| FauNDI | CATATG | 1 cut(s) | 503 |
| Fnu4HI | GCNGC | 5 cut(s) | 16, 125, 261, 313, 481 |
| FokI | GGATG | 4 cut(s) | 161, 200, 344, 423 |
| FriOI | GRGCYC | 2 cut(s) | 156, 276 |
| Fsp4HI | GCNGC | 5 cut(s) | 16, 125, 261, 313, 481 |
| FspBI | CTAG | 3 cut(s) | 228, 234, 342 |
| GlaI | GCGC | 2 cut(s) | 40, 303 |
| GluI | GCNGC | 5 cut(s) | 16, 125, 261, 313, 481 |
| HaeIII | GGCC | 5 cut(s) | 154, 274, 297, 312, 404 |
| HapII | CCGG | 8 cut(s) | 36, 64, 100, 324, 359, 368, 405, 443 |
| HgaI | GACGC | 1 cut(s) | 384 |
| HhaI | GCGC | 2 cut(s) | 41, 304 |
| Hin1II | CATG | 2 cut(s) | 435, 487 |
| Hin6I | GCGC | 2 cut(s) | 39, 302 |
| HinP1I | GCGC | 2 cut(s) | 39, 302 |
| HincII | GTYRAC | 1 cut(s) | 75 |
| HindII | GTYRAC | 1 cut(s) | 75 |
| HinfI | GANTC | 2 cut(s) | 197, 306 |
| HpaII | CCGG | 8 cut(s) | 36, 64, 100, 324, 359, 368, 405, 443 |
| HphI | GGTGA | 1 cut(s) | 499 |
| Hpy166II | GTNNAC | 1 cut(s) | 75 |
| Hpy188I | TCNGA | 1 cut(s) | 400 |
| Hpy8I | GTNNAC | 1 cut(s) | 75 |
| HpyAV | CCTTC | 3 cut(s) | 5, 235, 272 |
| HpyCH4III | ACNGT | 2 cut(s) | 72, 121 |
| HpyCH4V | TGCA | 3 cut(s) | 335, 431, 452 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 12, 21, 47, 133, 230, 401 |
| Hsp92II | CATG | 2 cut(s) | 435, 487 |
| HspAI | GCGC | 2 cut(s) | 39, 302 |
| KpnI | GGTACC | 1 cut(s) | 99 |
| KroI | GCCGGC | 1 cut(s) | 442 |
| KroNI | GCCGGC | 1 cut(s) | 444 |
| Kzo9I | GATC | 1 cut(s) | 424 |
| LmnI | GCTCC | 4 cut(s) | 58, 183, 267, 282 |
| Lsp1109I | GCAGC | 1 cut(s) | 111 |
| LweI | GCATC | 1 cut(s) | 322 |
| MaeI | CTAG | 3 cut(s) | 228, 234, 342 |
| MaeIII | GTNAC | 3 cut(s) | 198, 468, 487 |
| MalI | GATC | 1 cut(s) | 426 |
| MbiI | CCGCTC | 2 cut(s) | 219, 270 |
| MboI | GATC | 1 cut(s) | 424 |
| MboII | GAAGA | 2 cut(s) | 20, 382 |
| MflI | RGATCY | 1 cut(s) | 424 |
| MhlI | GDGCHC | 3 cut(s) | 63, 156, 276 |
| MlyI | GAGTC | 2 cut(s) | 206, 300 |
| MnlI | CCTC | 8 cut(s) | 14, 165, 170, 183, 263, 286, 296, 394 |
| MreI | CGCCGGCG | 1 cut(s) | 442 |
| MroNI | GCCGGC | 1 cut(s) | 442 |
| MseI | TTAA | 1 cut(s) | 318 |
| MspI | CCGG | 8 cut(s) | 36, 64, 100, 324, 359, 368, 405, 443 |
| MspR9I | CCNGG | 6 cut(s) | 36, 100, 299, 324, 368, 406 |
| MvaI | CCWGG | 1 cut(s) | 299 |
| MvnI | CGCG | 3 cut(s) | 56, 304, 395 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 12, 21, 47, 133, 230, 401 |
| NaeI | GCCGGC | 1 cut(s) | 444 |
| NciI | CCSGG | 5 cut(s) | 36, 100, 324, 368, 406 |
| NdeI | CATATG | 1 cut(s) | 503 |
| NdeII | GATC | 1 cut(s) | 424 |
| NgoMIV | GCCGGC | 1 cut(s) | 442 |
| NlaIII | CATG | 2 cut(s) | 435, 487 |
| NlaIV | GGNNCC | 6 cut(s) | 97, 154, 274, 284, 372, 409 |
| NmeAIII | GCCGAG | 4 cut(s) | 162, 200, 267, 288 |
| NmuCI | GTSAC | 3 cut(s) | 198, 468, 487 |
| NspI | RCATGY | 1 cut(s) | 435 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 125 |
| PdiI | GCCGGC | 1 cut(s) | 444 |
| PflFI | GACNNNGTC | 1 cut(s) | 70 |
| PfoI | TCCNGGA | 1 cut(s) | 322 |
| PkrI | GCNGC | 5 cut(s) | 17, 126, 262, 314, 482 |
| PleI | GAGTC | 2 cut(s) | 205, 300 |
| PpsI | GAGTC | 2 cut(s) | 205, 300 |
| Psp6I | CCWGG | 1 cut(s) | 297 |
| PspEI | GGTNACC | 1 cut(s) | 487 |
| PspGI | CCWGG | 1 cut(s) | 297 |
| PspN4I | GGNNCC | 6 cut(s) | 97, 154, 274, 284, 372, 409 |
| PspOMI | GGGCCC | 2 cut(s) | 152, 272 |
| PspPI | GGNCC | 7 cut(s) | 102, 152, 153, 272, 273, 295, 408 |
| PsuI | RGATCY | 1 cut(s) | 424 |
| PsyI | GACNNNGTC | 1 cut(s) | 70 |
| RsaI | GTAC | 1 cut(s) | 97 |
| RsaNI | GTAC | 1 cut(s) | 96 |
| SaqAI | TTAA | 1 cut(s) | 318 |
| SatI | GCNGC | 5 cut(s) | 16, 125, 261, 313, 481 |
| Sau3AI | GATC | 1 cut(s) | 424 |
| Sau96I | GGNCC | 7 cut(s) | 102, 152, 153, 272, 273, 295, 408 |
| SchI | GAGTC | 2 cut(s) | 206, 300 |
| ScrFI | CCNGG | 6 cut(s) | 36, 100, 299, 324, 368, 406 |
| SduI | GDGCHC | 3 cut(s) | 63, 156, 276 |
| SetI | ASST | 2 cut(s) | 255, 514 |
| SfaNI | GCATC | 1 cut(s) | 322 |
| SgrAI | CRCCGGYG | 1 cut(s) | 442 |
| SinI | GGWCC | 2 cut(s) | 102, 408 |
| SspMI | CTAG | 3 cut(s) | 228, 234, 342 |
| StyD4I | CCNGG | 6 cut(s) | 34, 98, 297, 322, 366, 404 |
| TaaI | ACNGT | 2 cut(s) | 72, 121 |
| TauI | GCSGC | 4 cut(s) | 18, 263, 315, 483 |
| Tru1I | TTAA | 1 cut(s) | 318 |
| Tru9I | TTAA | 1 cut(s) | 318 |
| TseFI | GTSAC | 3 cut(s) | 198, 468, 487 |
| TseI | GCWGC | 1 cut(s) | 124 |
| Tsp45I | GTSAC | 3 cut(s) | 198, 468, 487 |
| Tth111I | GACNNNGTC | 1 cut(s) | 70 |
| VpaK11BI | GGWCC | 2 cut(s) | 102, 408 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 290 |
| XceI | RCATGY | 1 cut(s) | 435 |
| XspI | CTAG | 3 cut(s) | 228, 234, 342 |