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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 691 |
| Acc36I | ACCTGC | 2 cut(s) | 538, 691 |
| Acc65I | GGTACC | 2 cut(s) | 523, 550 |
| AccB1I | GGYRCC | 3 cut(s) | 523, 550, 803 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 512, 754 |
| AccBSI | CCGCTC | 1 cut(s) | 648 |
| AccI | GTMKAC | 2 cut(s) | 108, 502 |
| AccII | CGCG | 2 cut(s) | 113, 363 |
| AclWI | GGATC | 4 cut(s) | 96, 245, 345, 781 |
| AcyI | GRCGYC | 1 cut(s) | 804 |
| AfaI | GTAC | 5 cut(s) | 56, 320, 525, 540, 552 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 66, 147, 148, 215, 260, 295, 512, 754, 846 |
| AjiI | CACGTC | 1 cut(s) | 422 |
| AjnI | CCWGG | 4 cut(s) | 4, 491, 505, 844 |
| AluBI | AGCT | 5 cut(s) | 138, 147, 325, 656, 772 |
| AluI | AGCT | 5 cut(s) | 138, 147, 325, 656, 772 |
| Alw26I | GTCTC | 2 cut(s) | 25, 685 |
| AlwI | GGATC | 4 cut(s) | 96, 245, 345, 781 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 512, 754 |
| AoxI | GGCC | 3 cut(s) | 298, 390, 848 |
| ApaI | GGGCCC | 1 cut(s) | 852 |
| ApeKI | GCWGC | 4 cut(s) | 219, 282, 285, 860 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 161 |
| Asp718I | GGTACC | 2 cut(s) | 523, 550 |
| AspLEI | GCGC | 6 cut(s) | 115, 469, 512, 688, 806, 814 |
| AspS9I | GGNCC | 6 cut(s) | 298, 391, 584, 703, 848, 849 |
| AsuC2I | CCSGG | 3 cut(s) | 262, 297, 791 |
| AsuHPI | GGTGA | 5 cut(s) | 83, 94, 198, 672, 847 |
| AvaII | GGWCC | 2 cut(s) | 584, 703 |
| BaeGI | GKGCMC | 1 cut(s) | 852 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 507, 540 |
| BanI | GGYRCC | 3 cut(s) | 523, 550, 803 |
| BanII | GRGCYC | 1 cut(s) | 852 |
| BauI | CACGAG | 1 cut(s) | 241 |
| BbvCI | CCTCAGC | 2 cut(s) | 79, 387 |
| BbvI | GCAGC | 4 cut(s) | 231, 294, 297, 847 |
| BccI | CCATC | 2 cut(s) | 574, 602 |
| BceAI | ACGGC | 1 cut(s) | 100 |
| BciT130I | CCWGG | 4 cut(s) | 6, 493, 507, 846 |
| BciVI | GTATCC | 1 cut(s) | 756 |
| BcnI | CCSGG | 3 cut(s) | 262, 297, 791 |
| BcoDI | GTCTC | 2 cut(s) | 25, 685 |
| BfaI | CTAG | 4 cut(s) | 63, 557, 611, 657 |
| BfoI | RGCGCY | 3 cut(s) | 513, 807, 815 |
| BfuAI | ACCTGC | 2 cut(s) | 538, 691 |
| BfuI | GTATCC | 1 cut(s) | 756 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 126, 297 |
| BglII | AGATCT | 1 cut(s) | 304 |
| BisI | GCNGC | 6 cut(s) | 220, 283, 286, 289, 635, 861 |
| BlsI | GCNGC | 6 cut(s) | 221, 284, 287, 290, 636, 862 |
| Bme1390I | CCNGG | 7 cut(s) | 6, 262, 297, 493, 507, 791, 846 |
| Bme18I | GGWCC | 2 cut(s) | 584, 703 |
| BmgBI | CACGTC | 1 cut(s) | 422 |
| BmgT120I | GGNCC | 6 cut(s) | 298, 391, 584, 703, 848, 849 |
| BmiI | GGNNCC | 6 cut(s) | 451, 525, 552, 585, 805, 850 |
| BmrFI | CCNGG | 7 cut(s) | 6, 262, 297, 493, 507, 791, 846 |
| BmsI | GCATC | 4 cut(s) | 138, 246, 534, 727 |
| BpmI | CTGGAG | 1 cut(s) | 794 |
| Bpu10I | CCTNAGC | 2 cut(s) | 79, 387 |
| BpuMI | CCSGG | 3 cut(s) | 262, 297, 791 |
| Bsa29I | ATCGAT | 1 cut(s) | 249 |
| BsaAI | YACGTR | 1 cut(s) | 485 |
| BsaHI | GRCGYC | 1 cut(s) | 804 |
| BsaJI | CCNNGG | 2 cut(s) | 5, 845 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 767, 797 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 66, 147, 148, 215, 260, 295, 512, 754, 846 |
| Bse118I | RCCGGY | 1 cut(s) | 731 |
| Bse1I | ACTGG | 3 cut(s) | 336, 533, 759 |
| BseBI | CCWGG | 4 cut(s) | 6, 493, 507, 846 |
| BseCI | ATCGAT | 1 cut(s) | 249 |
| BseDI | CCNNGG | 2 cut(s) | 5, 845 |
| BseGI | GGATG | 2 cut(s) | 585, 594 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 66, 147, 148, 215, 260, 295, 512, 754, 846 |
| BseMII | CTCAG | 5 cut(s) | 36, 93, 378, 558, 833 |
| BseNI | ACTGG | 3 cut(s) | 336, 533, 759 |
| BseRI | GAGGAG | 3 cut(s) | 187, 380, 383 |
| BseSI | GKGCMC | 1 cut(s) | 852 |
| BseXI | GCAGC | 4 cut(s) | 231, 294, 297, 847 |
| BseYI | CCCAGC | 3 cut(s) | 147, 445, 808 |
| Bsh1236I | CGCG | 2 cut(s) | 113, 363 |
| BshFI | GGCC | 3 cut(s) | 300, 392, 850 |
| BshNI | GGYRCC | 3 cut(s) | 523, 550, 803 |
| BshVI | ATCGAT | 1 cut(s) | 249 |
| BsiSI | CCGG | 4 cut(s) | 261, 296, 732, 790 |
| BslFI | GGGAC | 2 cut(s) | 153, 597 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 66, 147, 148, 215, 260, 295, 512, 754, 846 |
| BsmAI | GTCTC | 2 cut(s) | 25, 685 |
| BsmBI | CGTCTC | 1 cut(s) | 685 |
| BsmFI | GGGAC | 2 cut(s) | 153, 597 |
| BsnI | GGCC | 3 cut(s) | 300, 392, 850 |
| Bsp120I | GGGCCC | 1 cut(s) | 848 |
| Bsp1286I | GDGCHC | 1 cut(s) | 852 |
| Bsp143I | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| BspANI | GGCC | 3 cut(s) | 300, 392, 850 |
| BspCNI | CTCAG | 5 cut(s) | 35, 92, 379, 559, 832 |
| BspDI | ATCGAT | 1 cut(s) | 249 |
| BspFNI | CGCG | 2 cut(s) | 113, 363 |
| BspLI | GGNNCC | 6 cut(s) | 451, 525, 552, 585, 805, 850 |
| BspMI | ACCTGC | 2 cut(s) | 538, 691 |
| BspPI | GGATC | 4 cut(s) | 96, 245, 345, 781 |
| BspT107I | GGYRCC | 3 cut(s) | 523, 550, 803 |
| BsrBI | CCGCTC | 1 cut(s) | 648 |
| BsrFI | RCCGGY | 1 cut(s) | 731 |
| BsrI | ACTGG | 3 cut(s) | 336, 533, 759 |
| BssAI | RCCGGY | 1 cut(s) | 731 |
| BssECI | CCNNGG | 2 cut(s) | 5, 845 |
| BssMI | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| BssNI | GRCGYC | 1 cut(s) | 804 |
| BssSI | CACGAG | 1 cut(s) | 241 |
| Bst2BI | CACGAG | 1 cut(s) | 241 |
| Bst2UI | CCWGG | 4 cut(s) | 6, 493, 507, 846 |
| Bst4CI | ACNGT | 3 cut(s) | 106, 402, 480 |
| Bst6I | CTCTTC | 2 cut(s) | 205, 841 |
| BstACI | GRCGYC | 1 cut(s) | 804 |
| BstBAI | YACGTR | 1 cut(s) | 485 |
| BstC8I | GCNNGC | 5 cut(s) | 69, 293, 327, 514, 733 |
| BstDEI | CTNAG | 5 cut(s) | 22, 79, 387, 567, 819 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 213 |
| BstF5I | GGATG | 2 cut(s) | 585, 594 |
| BstFNI | CGCG | 2 cut(s) | 113, 363 |
| BstH2I | RGCGCY | 3 cut(s) | 513, 807, 815 |
| BstHHI | GCGC | 6 cut(s) | 115, 469, 512, 688, 806, 814 |
| BstKTI | GATC | 6 cut(s) | 91, 102, 253, 307, 340, 789 |
| BstMAI | GTCTC | 2 cut(s) | 25, 685 |
| BstMBI | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| BstNI | CCWGG | 4 cut(s) | 6, 493, 507, 846 |
| BstSCI | CCNGG | 7 cut(s) | 4, 260, 295, 491, 505, 789, 844 |
| BstSLI | GKGCMC | 1 cut(s) | 852 |
| BstUI | CGCG | 2 cut(s) | 113, 363 |
| BstV1I | GCAGC | 4 cut(s) | 231, 294, 297, 847 |
| BstX2I | RGATCY | 2 cut(s) | 304, 337 |
| BstYI | RGATCY | 2 cut(s) | 304, 337 |
| Bsu15I | ATCGAT | 1 cut(s) | 249 |
| BsuI | GTATCC | 1 cut(s) | 756 |
| BsuRI | GGCC | 3 cut(s) | 300, 392, 850 |
| BsuTUI | ATCGAT | 1 cut(s) | 249 |
| BtgZI | GCGATG | 1 cut(s) | 750 |
| BtrI | CACGTC | 1 cut(s) | 422 |
| BtsCI | GGATG | 2 cut(s) | 585, 594 |
| BtsI | GCAGTG | 1 cut(s) | 629 |
| BtsIMutI | CAGTG | 2 cut(s) | 329, 629 |
| BveI | ACCTGC | 2 cut(s) | 538, 691 |
| Cac8I | GCNNGC | 5 cut(s) | 69, 293, 327, 514, 733 |
| CaiI | CAGNNNCTG | 2 cut(s) | 512, 754 |
| CfoI | GCGC | 6 cut(s) | 115, 469, 512, 688, 806, 814 |
| Cfr10I | RCCGGY | 1 cut(s) | 731 |
| Cfr13I | GGNCC | 6 cut(s) | 298, 391, 584, 703, 848, 849 |
| ClaI | ATCGAT | 1 cut(s) | 249 |
| CseI | GACGC | 4 cut(s) | 72, 119, 451, 702 |
| Csp6I | GTAC | 5 cut(s) | 55, 319, 524, 539, 551 |
| CviQI | GTAC | 5 cut(s) | 55, 319, 524, 539, 551 |
| DdeI | CTNAG | 5 cut(s) | 22, 79, 387, 567, 819 |
| DinI | GGCGCC | 1 cut(s) | 805 |
| DpnI | GATC | 6 cut(s) | 90, 101, 252, 306, 339, 788 |
| DpnII | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| Eam1104I | CTCTTC | 2 cut(s) | 205, 841 |
| EarI | CTCTTC | 2 cut(s) | 205, 841 |
| EciI | GGCGGA | 3 cut(s) | 243, 350, 638 |
| Eco24I | GRGCYC | 1 cut(s) | 852 |
| Eco47I | GGWCC | 2 cut(s) | 584, 703 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 213 |
| EcoO109I | RGGNCCY | 1 cut(s) | 849 |
| EcoRII | CCWGG | 4 cut(s) | 4, 491, 505, 844 |
| EcoT38I | GRGCYC | 1 cut(s) | 852 |
| EgeI | GGCGCC | 1 cut(s) | 805 |
| EheI | GGCGCC | 1 cut(s) | 805 |
| Esp3I | CGTCTC | 1 cut(s) | 685 |
| FaiI | YATR | 5 cut(s) | 49, 212, 231, 317, 345 |
| FaqI | GGGAC | 2 cut(s) | 153, 597 |
| FauI | CCCGC | 2 cut(s) | 60, 300 |
| FblI | GTMKAC | 2 cut(s) | 108, 502 |
| Fnu4HI | GCNGC | 6 cut(s) | 220, 283, 286, 289, 635, 861 |
| FokI | GGATG | 2 cut(s) | 581, 592 |
| FriOI | GRGCYC | 1 cut(s) | 852 |
| Fsp4HI | GCNGC | 6 cut(s) | 220, 283, 286, 289, 635, 861 |
| FspBI | CTAG | 4 cut(s) | 63, 557, 611, 657 |
| GlaI | GCGC | 6 cut(s) | 114, 468, 511, 687, 805, 813 |
| GluI | GCNGC | 6 cut(s) | 220, 283, 286, 289, 635, 861 |
| GsaI | CCCAGC | 3 cut(s) | 151, 449, 812 |
| GsuI | CTGGAG | 1 cut(s) | 794 |
| HaeII | RGCGCY | 3 cut(s) | 513, 807, 815 |
| HaeIII | GGCC | 3 cut(s) | 300, 392, 850 |
| HapII | CCGG | 4 cut(s) | 261, 296, 732, 790 |
| HgaI | GACGC | 4 cut(s) | 72, 119, 451, 702 |
| HhaI | GCGC | 6 cut(s) | 115, 469, 512, 688, 806, 814 |
| Hin1I | GRCGYC | 1 cut(s) | 804 |
| Hin6I | GCGC | 6 cut(s) | 113, 467, 510, 686, 804, 812 |
| HinP1I | GCGC | 6 cut(s) | 113, 467, 510, 686, 804, 812 |
| HincII | GTYRAC | 2 cut(s) | 109, 503 |
| HindII | GTYRAC | 2 cut(s) | 109, 503 |
| HinfI | GANTC | 5 cut(s) | 18, 626, 797, 823, 865 |
| HpaII | CCGG | 4 cut(s) | 261, 296, 732, 790 |
| HphI | GGTGA | 5 cut(s) | 83, 94, 198, 672, 847 |
| Hpy166II | GTNNAC | 3 cut(s) | 57, 109, 503 |
| Hpy188I | TCNGA | 3 cut(s) | 88, 652, 822 |
| Hpy188III | TCNNGA | 5 cut(s) | 15, 24, 241, 425, 840 |
| Hpy8I | GTNNAC | 3 cut(s) | 57, 109, 503 |
| Hpy99I | CGWCG | 3 cut(s) | 88, 110, 113 |
| HpyAV | CCTTC | 2 cut(s) | 376, 862 |
| HpyCH4III | ACNGT | 3 cut(s) | 106, 402, 480 |
| HpyCH4IV | ACGT | 2 cut(s) | 421, 484 |
| HpyCH4V | TGCA | 2 cut(s) | 547, 740 |
| HpyF3I | CTNAG | 5 cut(s) | 22, 79, 387, 567, 819 |
| HpySE526I | ACGT | 2 cut(s) | 421, 484 |
| Hsp92I | GRCGYC | 1 cut(s) | 804 |
| HspAI | GCGC | 6 cut(s) | 113, 467, 510, 686, 804, 812 |
| KasI | GGCGCC | 1 cut(s) | 803 |
| KpnI | GGTACC | 2 cut(s) | 527, 554 |
| KroI | GCCGGC | 1 cut(s) | 731 |
| KroNI | GCCGGC | 1 cut(s) | 733 |
| Kzo9I | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| LmnI | GCTCC | 4 cut(s) | 143, 370, 449, 775 |
| Lsp1109I | GCAGC | 4 cut(s) | 231, 294, 297, 847 |
| LweI | GCATC | 4 cut(s) | 138, 246, 534, 727 |
| MaeI | CTAG | 4 cut(s) | 63, 557, 611, 657 |
| MaeII | ACGT | 2 cut(s) | 421, 484 |
| MaeIII | GTNAC | 2 cut(s) | 417, 480 |
| MalI | GATC | 6 cut(s) | 90, 101, 252, 306, 339, 788 |
| MbiI | CCGCTC | 1 cut(s) | 648 |
| MboI | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| MboII | GAAGA | 4 cut(s) | 192, 201, 332, 828 |
| MflI | RGATCY | 2 cut(s) | 304, 337 |
| MhlI | GDGCHC | 1 cut(s) | 852 |
| MluCI | AATT | 1 cut(s) | 676 |
| Mly113I | GGCGCC | 1 cut(s) | 804 |
| MlyI | GAGTC | 2 cut(s) | 27, 806 |
| MmeI | TCCRAC | 1 cut(s) | 66 |
| MreI | CGCCGGCG | 1 cut(s) | 731 |
| MroNI | GCCGGC | 1 cut(s) | 731 |
| MroXI | GAANNNNTTC | 1 cut(s) | 161 |
| MslI | CAYNNNNRTG | 1 cut(s) | 314 |
| MspA1I | CMGCKG | 5 cut(s) | 222, 269, 709, 772, 860 |
| MspI | CCGG | 4 cut(s) | 261, 296, 732, 790 |
| MspR9I | CCNGG | 7 cut(s) | 6, 262, 297, 493, 507, 791, 846 |
| MvaI | CCWGG | 4 cut(s) | 6, 493, 507, 846 |
| MvnI | CGCG | 2 cut(s) | 113, 363 |
| NaeI | GCCGGC | 1 cut(s) | 733 |
| NarI | GGCGCC | 1 cut(s) | 804 |
| NciI | CCSGG | 3 cut(s) | 262, 297, 791 |
| NdeII | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| NgoMIV | GCCGGC | 1 cut(s) | 731 |
| NlaIV | GGNNCC | 6 cut(s) | 451, 525, 552, 585, 805, 850 |
| NmeAIII | GCCGAG | 1 cut(s) | 714 |
| NmuCI | GTSAC | 2 cut(s) | 417, 480 |
| PaqCI | CACCTGC | 1 cut(s) | 691 |
| PdiI | GCCGGC | 1 cut(s) | 733 |
| PdmI | GAANNNNTTC | 1 cut(s) | 161 |
| PfeI | GAWTC | 3 cut(s) | 626, 823, 865 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 512, 754 |
| PkrI | GCNGC | 6 cut(s) | 221, 284, 287, 290, 636, 862 |
| PleI | GAGTC | 2 cut(s) | 26, 805 |
| PluTI | GGCGCC | 1 cut(s) | 807 |
| PpsI | GAGTC | 2 cut(s) | 26, 805 |
| Ppu21I | YACGTR | 1 cut(s) | 485 |
| Psp6I | CCWGG | 4 cut(s) | 4, 491, 505, 844 |
| PspFI | CCCAGC | 3 cut(s) | 147, 445, 808 |
| PspGI | CCWGG | 4 cut(s) | 4, 491, 505, 844 |
| PspN4I | GGNNCC | 6 cut(s) | 451, 525, 552, 585, 805, 850 |
| PspOMI | GGGCCC | 1 cut(s) | 848 |
| PspPI | GGNCC | 6 cut(s) | 298, 391, 584, 703, 848, 849 |
| PstNI | CAGNNNCTG | 2 cut(s) | 512, 754 |
| PsuI | RGATCY | 2 cut(s) | 304, 337 |
| PvuII | CAGCTG | 1 cut(s) | 772 |
| RsaI | GTAC | 5 cut(s) | 56, 320, 525, 540, 552 |
| RsaNI | GTAC | 5 cut(s) | 55, 319, 524, 539, 551 |
| RseI | CAYNNNNRTG | 1 cut(s) | 314 |
| SalI | GTCGAC | 2 cut(s) | 107, 501 |
| SatI | GCNGC | 6 cut(s) | 220, 283, 286, 289, 635, 861 |
| Sau3AI | GATC | 6 cut(s) | 88, 99, 250, 304, 337, 786 |
| Sau96I | GGNCC | 6 cut(s) | 298, 391, 584, 703, 848, 849 |
| SchI | GAGTC | 2 cut(s) | 27, 806 |
| ScrFI | CCNGG | 7 cut(s) | 6, 262, 297, 493, 507, 791, 846 |
| SduI | GDGCHC | 1 cut(s) | 852 |
| SfaNI | GCATC | 4 cut(s) | 138, 246, 534, 727 |
| SfoI | GGCGCC | 1 cut(s) | 805 |
| SgrAI | CRCCGGYG | 1 cut(s) | 731 |
| SgrDI | CGTCGACG | 1 cut(s) | 107 |
| SinI | GGWCC | 2 cut(s) | 584, 703 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 314 |
| Sse9I | AATT | 1 cut(s) | 676 |
| SspDI | GGCGCC | 1 cut(s) | 803 |
| SspMI | CTAG | 4 cut(s) | 63, 557, 611, 657 |
| StyD4I | CCNGG | 7 cut(s) | 4, 260, 295, 491, 505, 789, 844 |
| TaaI | ACNGT | 3 cut(s) | 106, 402, 480 |
| TaiI | ACGT | 2 cut(s) | 424, 487 |
| TaqI | TCGA | 4 cut(s) | 108, 176, 249, 502 |
| TasI | AATT | 1 cut(s) | 676 |
| TauI | GCSGC | 2 cut(s) | 291, 637 |
| TfiI | GAWTC | 3 cut(s) | 626, 823, 865 |
| TscAI | CASTG | 2 cut(s) | 336, 636 |
| TseFI | GTSAC | 2 cut(s) | 417, 480 |
| TseI | GCWGC | 4 cut(s) | 219, 282, 285, 860 |
| Tsp45I | GTSAC | 2 cut(s) | 417, 480 |
| TspDTI | ATGAA | 2 cut(s) | 332, 444 |
| TspRI | CASTG | 2 cut(s) | 336, 636 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 512, 754 |
| VpaK11BI | GGWCC | 2 cut(s) | 584, 703 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 213 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 751 |
| XmiI | GTMKAC | 2 cut(s) | 108, 502 |
| XmnI | GAANNNNTTC | 1 cut(s) | 161 |
| XspI | CTAG | 4 cut(s) | 63, 557, 611, 657 |