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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 556 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 3 cut(s) | 74, 90, 719 |
| AclWI | GGATC | 5 cut(s) | 168, 201, 527, 715, 782 |
| AcoI | YGGCCR | 1 cut(s) | 303 |
| AcuI | CTGAAG | 3 cut(s) | 48, 873, 914 |
| AcyI | GRCGYC | 1 cut(s) | 660 |
| AdeI | CACNNNGTG | 1 cut(s) | 871 |
| AfaI | GTAC | 3 cut(s) | 176, 408, 996 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 610 |
| AgsI | TTSAA | 2 cut(s) | 508, 814 |
| AjiI | CACGTC | 1 cut(s) | 1004 |
| AjnI | CCWGG | 8 cut(s) | 115, 133, 151, 156, 249, 347, 382, 421 |
| AluBI | AGCT | 2 cut(s) | 43, 504 |
| AluI | AGCT | 2 cut(s) | 43, 504 |
| Alw21I | GWGCWC | 1 cut(s) | 45 |
| Alw26I | GTCTC | 1 cut(s) | 647 |
| AlwI | GGATC | 5 cut(s) | 168, 201, 527, 715, 782 |
| ApeKI | GCWGC | 6 cut(s) | 138, 141, 263, 342, 377, 882 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 5 cut(s) | 92, 268, 368, 382, 552 |
| AspS9I | GGNCC | 4 cut(s) | 247, 386, 704, 951 |
| AsuC2I | CCSGG | 2 cut(s) | 647, 702 |
| AsuHPI | GGTGA | 4 cut(s) | 160, 542, 814, 957 |
| AvaII | GGWCC | 1 cut(s) | 951 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| BbsI | GAAGAC | 1 cut(s) | 41 |
| Bbv12I | GWGCWC | 1 cut(s) | 45 |
| BbvI | GCAGC | 6 cut(s) | 150, 153, 250, 354, 389, 894 |
| BccI | CCATC | 3 cut(s) | 59, 175, 253 |
| BceAI | ACGGC | 1 cut(s) | 716 |
| BciT130I | CCWGG | 8 cut(s) | 117, 135, 153, 158, 251, 349, 384, 423 |
| BcnI | CCSGG | 2 cut(s) | 647, 702 |
| BcoDI | GTCTC | 1 cut(s) | 647 |
| BfaI | CTAG | 2 cut(s) | 546, 903 |
| BfmI | CTRYAG | 1 cut(s) | 725 |
| BfoI | RGCGCY | 2 cut(s) | 369, 383 |
| BfuAI | ACCTGC | 1 cut(s) | 556 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 562 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| Bme18I | GGWCC | 1 cut(s) | 951 |
| BmgBI | CACGTC | 1 cut(s) | 1004 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 386, 704, 951 |
| BmiI | GGNNCC | 3 cut(s) | 680, 706, 952 |
| BmrI | ACTGGG | 1 cut(s) | 598 |
| BmsI | GCATC | 3 cut(s) | 609, 894, 927 |
| BmuI | ACTGGG | 1 cut(s) | 598 |
| BoxI | GACNNNNGTC | 1 cut(s) | 659 |
| BpiI | GAAGAC | 1 cut(s) | 41 |
| BpmI | CTGGAG | 1 cut(s) | 650 |
| BpuEI | CTTGAG | 1 cut(s) | 930 |
| BpuMI | CCSGG | 2 cut(s) | 647, 702 |
| BsaAI | YACGTR | 1 cut(s) | 406 |
| BsaHI | GRCGYC | 1 cut(s) | 660 |
| BsaJI | CCNNGG | 6 cut(s) | 115, 249, 383, 422, 545, 913 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 610 |
| Bse118I | RCCGGY | 3 cut(s) | 368, 388, 587 |
| Bse1I | ACTGG | 3 cut(s) | 192, 604, 633 |
| BseBI | CCWGG | 8 cut(s) | 117, 135, 153, 158, 251, 349, 384, 423 |
| BseDI | CCNNGG | 6 cut(s) | 115, 249, 383, 422, 545, 913 |
| BseGI | GGATG | 3 cut(s) | 51, 624, 980 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 610 |
| BseMII | CTCAG | 1 cut(s) | 516 |
| BseNI | ACTGG | 3 cut(s) | 192, 604, 633 |
| BseRI | GAGGAG | 1 cut(s) | 933 |
| BseXI | GCAGC | 6 cut(s) | 150, 153, 250, 354, 389, 894 |
| BseYI | CCCAGC | 1 cut(s) | 362 |
| Bsh1236I | CGCG | 3 cut(s) | 74, 90, 719 |
| BsiHKAI | GWGCWC | 1 cut(s) | 45 |
| BsiSI | CCGG | 5 cut(s) | 369, 389, 588, 646, 702 |
| BsiWI | CGTACG | 1 cut(s) | 994 |
| BslFI | GGGAC | 3 cut(s) | 453, 647, 964 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 546, 610 |
| BsmAI | GTCTC | 1 cut(s) | 647 |
| BsmBI | CGTCTC | 1 cut(s) | 647 |
| BsmFI | GGGAC | 3 cut(s) | 453, 647, 964 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp143I | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| Bsp19I | CCATGG | 1 cut(s) | 913 |
| BspCNI | CTCAG | 1 cut(s) | 517 |
| BspFNI | CGCG | 3 cut(s) | 74, 90, 719 |
| BspHI | TCATGA | 1 cut(s) | 1006 |
| BspLI | GGNNCC | 3 cut(s) | 680, 706, 952 |
| BspMI | ACCTGC | 1 cut(s) | 556 |
| BspPI | GGATC | 5 cut(s) | 168, 201, 527, 715, 782 |
| BsrFI | RCCGGY | 3 cut(s) | 368, 388, 587 |
| BsrI | ACTGG | 3 cut(s) | 192, 604, 633 |
| BssAI | RCCGGY | 3 cut(s) | 368, 388, 587 |
| BssECI | CCNNGG | 6 cut(s) | 115, 249, 383, 422, 545, 913 |
| BssMI | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| BssNI | GRCGYC | 1 cut(s) | 660 |
| BssT1I | CCWWGG | 2 cut(s) | 545, 913 |
| Bst2UI | CCWGG | 8 cut(s) | 117, 135, 153, 158, 251, 349, 384, 423 |
| Bst4CI | ACNGT | 1 cut(s) | 955 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 1 cut(s) | 660 |
| BstBAI | YACGTR | 1 cut(s) | 406 |
| BstC8I | GCNNGC | 6 cut(s) | 149, 332, 370, 554, 589, 877 |
| BstDEI | CTNAG | 1 cut(s) | 525 |
| BstDSI | CCRYGG | 1 cut(s) | 913 |
| BstEII | GGTNACC | 1 cut(s) | 820 |
| BstF5I | GGATG | 3 cut(s) | 51, 624, 980 |
| BstFNI | CGCG | 3 cut(s) | 74, 90, 719 |
| BstH2I | RGCGCY | 2 cut(s) | 369, 383 |
| BstHHI | GCGC | 5 cut(s) | 92, 268, 368, 382, 552 |
| BstKTI | GATC | 5 cut(s) | 163, 196, 535, 723, 790 |
| BstMAI | GTCTC | 1 cut(s) | 647 |
| BstMBI | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| BstNI | CCWGG | 8 cut(s) | 117, 135, 153, 158, 251, 349, 384, 423 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 659 |
| BstPI | GGTNACC | 1 cut(s) | 820 |
| BstSFI | CTRYAG | 1 cut(s) | 725 |
| BstUI | CGCG | 3 cut(s) | 74, 90, 719 |
| BstV1I | GCAGC | 6 cut(s) | 150, 153, 250, 354, 389, 894 |
| BstV2I | GAAGAC | 1 cut(s) | 41 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BtgI | CCRYGG | 1 cut(s) | 913 |
| BtrI | CACGTC | 1 cut(s) | 1004 |
| BtsCI | GGATG | 3 cut(s) | 51, 624, 980 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 849 |
| BveI | ACCTGC | 1 cut(s) | 556 |
| Cac8I | GCNNGC | 6 cut(s) | 149, 332, 370, 554, 589, 877 |
| CciI | TCATGA | 1 cut(s) | 1006 |
| CfoI | GCGC | 5 cut(s) | 92, 268, 368, 382, 552 |
| Cfr10I | RCCGGY | 3 cut(s) | 368, 388, 587 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 386, 704, 951 |
| CseI | GACGC | 1 cut(s) | 649 |
| Csp6I | GTAC | 3 cut(s) | 175, 407, 995 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 5 cut(s) | 172, 271, 802, 914, 1007 |
| CviQI | GTAC | 3 cut(s) | 175, 407, 995 |
| DdeI | CTNAG | 1 cut(s) | 525 |
| DpnI | GATC | 5 cut(s) | 162, 195, 534, 722, 789 |
| DpnII | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| DraIII | CACNNNGTG | 1 cut(s) | 871 |
| EaeI | YGGCCR | 1 cut(s) | 303 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Ecl136II | GAGCTC | 1 cut(s) | 43 |
| Eco130I | CCWWGG | 2 cut(s) | 545, 913 |
| Eco147I | AGGCCT | 1 cut(s) | 858 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco47I | GGWCC | 1 cut(s) | 951 |
| Eco53kI | GAGCTC | 1 cut(s) | 43 |
| Eco57I | CTGAAG | 3 cut(s) | 48, 873, 914 |
| Eco91I | GGTNACC | 1 cut(s) | 820 |
| EcoICRI | GAGCTC | 1 cut(s) | 43 |
| EcoO65I | GGTNACC | 1 cut(s) | 820 |
| EcoRII | CCWGG | 8 cut(s) | 115, 133, 151, 156, 249, 347, 382, 421 |
| EcoT14I | CCWWGG | 2 cut(s) | 545, 913 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| ErhI | CCWWGG | 2 cut(s) | 545, 913 |
| Esp3I | CGTCTC | 1 cut(s) | 647 |
| FaeI | CATG | 5 cut(s) | 175, 274, 805, 917, 1010 |
| FaqI | GGGAC | 3 cut(s) | 453, 647, 964 |
| FatI | CATG | 5 cut(s) | 171, 270, 801, 913, 1006 |
| FauI | CCCGC | 5 cut(s) | 55, 79, 156, 690, 868 |
| FauNDI | CATATG | 1 cut(s) | 745 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| FokI | GGATG | 3 cut(s) | 38, 631, 967 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| FspBI | CTAG | 2 cut(s) | 546, 903 |
| GlaI | GCGC | 5 cut(s) | 91, 267, 367, 381, 551 |
| GsaI | CCCAGC | 1 cut(s) | 366 |
| GsuI | CTGGAG | 1 cut(s) | 650 |
| HaeII | RGCGCY | 2 cut(s) | 369, 383 |
| HapII | CCGG | 5 cut(s) | 369, 389, 588, 646, 702 |
| HgaI | GACGC | 1 cut(s) | 649 |
| HhaI | GCGC | 5 cut(s) | 92, 268, 368, 382, 552 |
| Hin1I | GRCGYC | 1 cut(s) | 660 |
| Hin1II | CATG | 5 cut(s) | 175, 274, 805, 917, 1010 |
| Hin6I | GCGC | 5 cut(s) | 90, 266, 366, 380, 550 |
| HinP1I | GCGC | 5 cut(s) | 90, 266, 366, 380, 550 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 4 cut(s) | 482, 581, 846, 940 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 5 cut(s) | 369, 389, 588, 646, 702 |
| HphI | GGTGA | 4 cut(s) | 160, 542, 814, 957 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 359, 690, 799 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 678 |
| Hpy188III | TCNNGA | 6 cut(s) | 432, 664, 696, 814, 932, 1007 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 359, 690, 799 |
| Hpy99I | CGWCG | 1 cut(s) | 995 |
| HpyAV | CCTTC | 6 cut(s) | 232, 497, 811, 820, 848, 938 |
| HpyCH4III | ACNGT | 1 cut(s) | 955 |
| HpyCH4IV | ACGT | 5 cut(s) | 217, 277, 405, 993, 1003 |
| HpyCH4V | TGCA | 3 cut(s) | 403, 622, 749 |
| HpyF3I | CTNAG | 1 cut(s) | 525 |
| HpySE526I | ACGT | 5 cut(s) | 217, 277, 405, 993, 1003 |
| Hsp92I | GRCGYC | 1 cut(s) | 660 |
| Hsp92II | CATG | 5 cut(s) | 175, 274, 805, 917, 1010 |
| HspAI | GCGC | 5 cut(s) | 90, 266, 366, 380, 550 |
| KroI | GCCGGC | 2 cut(s) | 368, 587 |
| KroNI | GCCGGC | 2 cut(s) | 370, 589 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| LmnI | GCTCC | 2 cut(s) | 48, 631 |
| Lsp1109I | GCAGC | 6 cut(s) | 150, 153, 250, 354, 389, 894 |
| LweI | GCATC | 3 cut(s) | 609, 894, 927 |
| MaeI | CTAG | 2 cut(s) | 546, 903 |
| MaeII | ACGT | 5 cut(s) | 217, 277, 405, 993, 1003 |
| MaeIII | GTNAC | 4 cut(s) | 166, 336, 820, 863 |
| MalI | GATC | 5 cut(s) | 162, 195, 534, 722, 789 |
| MboI | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| MboII | GAAGA | 2 cut(s) | 41, 46 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| MluCI | AATT | 1 cut(s) | 971 |
| MlyI | GAGTC | 1 cut(s) | 934 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MreI | CGCCGGCG | 1 cut(s) | 587 |
| MroNI | GCCGGC | 2 cut(s) | 368, 587 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MseI | TTAA | 2 cut(s) | 84, 641 |
| MslI | CAYNNNNRTG | 3 cut(s) | 398, 890, 985 |
| MspA1I | CMGCKG | 3 cut(s) | 15, 412, 685 |
| MspI | CCGG | 5 cut(s) | 369, 389, 588, 646, 702 |
| MvaI | CCWGG | 8 cut(s) | 117, 135, 153, 158, 251, 349, 384, 423 |
| MvnI | CGCG | 3 cut(s) | 74, 90, 719 |
| NaeI | GCCGGC | 2 cut(s) | 370, 589 |
| NciI | CCSGG | 2 cut(s) | 647, 702 |
| NcoI | CCATGG | 1 cut(s) | 913 |
| NdeI | CATATG | 1 cut(s) | 745 |
| NdeII | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| NgoMIV | GCCGGC | 2 cut(s) | 368, 587 |
| NlaIII | CATG | 5 cut(s) | 175, 274, 805, 917, 1010 |
| NlaIV | GGNNCC | 3 cut(s) | 680, 706, 952 |
| NmeAIII | GCCGAG | 1 cut(s) | 541 |
| NmuCI | GTSAC | 4 cut(s) | 166, 336, 820, 863 |
| PagI | TCATGA | 1 cut(s) | 1006 |
| PceI | AGGCCT | 1 cut(s) | 858 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 662 |
| PdiI | GCCGGC | 2 cut(s) | 370, 589 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 3 cut(s) | 482, 581, 846 |
| Pfl23II | CGTACG | 1 cut(s) | 994 |
| PflFI | GACNNNGTC | 1 cut(s) | 1002 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PleI | GAGTC | 1 cut(s) | 934 |
| PpsI | GAGTC | 1 cut(s) | 934 |
| Ppu21I | YACGTR | 1 cut(s) | 406 |
| PshAI | GACNNNNGTC | 1 cut(s) | 659 |
| Psp124BI | GAGCTC | 1 cut(s) | 45 |
| Psp6I | CCWGG | 8 cut(s) | 115, 133, 151, 156, 249, 347, 382, 421 |
| PspEI | GGTNACC | 1 cut(s) | 820 |
| PspFI | CCCAGC | 1 cut(s) | 362 |
| PspGI | CCWGG | 8 cut(s) | 115, 133, 151, 156, 249, 347, 382, 421 |
| PspLI | CGTACG | 1 cut(s) | 994 |
| PspN4I | GGNNCC | 3 cut(s) | 680, 706, 952 |
| PspPI | GGNCC | 4 cut(s) | 247, 386, 704, 951 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PsyI | GACNNNGTC | 1 cut(s) | 1002 |
| RsaI | GTAC | 3 cut(s) | 176, 408, 996 |
| RsaNI | GTAC | 3 cut(s) | 175, 407, 995 |
| RseI | CAYNNNNRTG | 3 cut(s) | 398, 890, 985 |
| SacI | GAGCTC | 1 cut(s) | 45 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SaqAI | TTAA | 2 cut(s) | 84, 641 |
| Sau3AI | GATC | 5 cut(s) | 160, 193, 532, 720, 787 |
| Sau96I | GGNCC | 4 cut(s) | 247, 386, 704, 951 |
| SchI | GAGTC | 1 cut(s) | 934 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SfaNI | GCATC | 3 cut(s) | 609, 894, 927 |
| SfcI | CTRYAG | 1 cut(s) | 725 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SinI | GGWCC | 1 cut(s) | 951 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 398, 890, 985 |
| SmlI | CTYRAG | 1 cut(s) | 945 |
| SmoI | CTYRAG | 1 cut(s) | 945 |
| Sse9I | AATT | 1 cut(s) | 971 |
| SseBI | AGGCCT | 1 cut(s) | 858 |
| SspMI | CTAG | 2 cut(s) | 546, 903 |
| SstI | GAGCTC | 1 cut(s) | 45 |
| StuI | AGGCCT | 1 cut(s) | 858 |
| StyI | CCWWGG | 2 cut(s) | 545, 913 |
| TaaI | ACNGT | 1 cut(s) | 955 |
| TaiI | ACGT | 5 cut(s) | 220, 280, 408, 996, 1006 |
| TaqI | TCGA | 2 cut(s) | 282, 358 |
| TaqII | GACCGA | 1 cut(s) | 800 |
| TasI | AATT | 1 cut(s) | 971 |
| TauI | GCSGC | 6 cut(s) | 147, 308, 719, 882, 898, 927 |
| TfiI | GAWTC | 3 cut(s) | 482, 581, 846 |
| Tru1I | TTAA | 2 cut(s) | 84, 641 |
| Tru9I | TTAA | 2 cut(s) | 84, 641 |
| TscAI | CASTG | 2 cut(s) | 192, 856 |
| TseFI | GTSAC | 4 cut(s) | 166, 336, 820, 863 |
| TseI | GCWGC | 6 cut(s) | 138, 141, 263, 342, 377, 882 |
| Tsp45I | GTSAC | 4 cut(s) | 166, 336, 820, 863 |
| TspRI | CASTG | 2 cut(s) | 192, 856 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1002 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 951 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 178 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 2 cut(s) | 546, 903 |