No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 476 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 415 |
| AccBSI | CCGCTC | 1 cut(s) | 309 |
| AccI | GTMKAC | 1 cut(s) | 163 |
| AccII | CGCG | 3 cut(s) | 71, 145, 524 |
| AclWI | GGATC | 4 cut(s) | 332, 442, 520, 579 |
| AcoI | YGGCCR | 2 cut(s) | 66, 111 |
| AcyI | GRCGYC | 3 cut(s) | 465, 473, 643 |
| AdeI | CACNNNGTG | 1 cut(s) | 652 |
| AfaI | GTAC | 2 cut(s) | 201, 213 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 61, 173, 324, 351, 415 |
| AgsI | TTSAA | 1 cut(s) | 611 |
| AjnI | CCWGG | 5 cut(s) | 187, 226, 317, 378, 450 |
| AluBI | AGCT | 2 cut(s) | 412, 490 |
| AluI | AGCT | 2 cut(s) | 412, 490 |
| Alw26I | GTCTC | 1 cut(s) | 452 |
| AlwI | GGATC | 4 cut(s) | 332, 442, 520, 579 |
| AoxI | GGCC | 8 cut(s) | 45, 66, 111, 191, 372, 381, 508, 534 |
| ApeKI | GCWGC | 1 cut(s) | 147 |
| AspA2I | CCTAGG | 1 cut(s) | 350 |
| AspLEI | GCGC | 4 cut(s) | 36, 73, 147, 357 |
| AspS9I | GGNCC | 3 cut(s) | 191, 245, 509 |
| AsuC2I | CCSGG | 2 cut(s) | 125, 507 |
| AsuHPI | GGTGA | 2 cut(s) | 347, 611 |
| AvaII | GGWCC | 1 cut(s) | 245 |
| AvrII | CCTAGG | 1 cut(s) | 350 |
| BbvI | GCAGC | 1 cut(s) | 159 |
| BciT130I | CCWGG | 5 cut(s) | 189, 228, 319, 380, 452 |
| BcnI | CCSGG | 2 cut(s) | 125, 507 |
| BcoDI | GTCTC | 1 cut(s) | 452 |
| BfaI | CTAG | 1 cut(s) | 351 |
| BfmI | CTRYAG | 1 cut(s) | 530 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 367, 380 |
| BisI | GCNGC | 4 cut(s) | 69, 111, 148, 522 |
| BlnI | CCTAGG | 1 cut(s) | 350 |
| BlsI | GCNGC | 4 cut(s) | 70, 112, 149, 523 |
| Bme1390I | CCNGG | 7 cut(s) | 125, 189, 228, 319, 380, 452, 507 |
| Bme18I | GGWCC | 1 cut(s) | 245 |
| BmgT120I | GGNCC | 3 cut(s) | 191, 245, 509 |
| BmiI | GGNNCC | 3 cut(s) | 246, 485, 511 |
| BmrFI | CCNGG | 7 cut(s) | 125, 189, 228, 319, 380, 452, 507 |
| BmsI | GCATC | 3 cut(s) | 174, 388, 414 |
| BoxI | GACNNNNGTC | 1 cut(s) | 464 |
| BpmI | CTGGAG | 1 cut(s) | 455 |
| BpuMI | CCSGG | 2 cut(s) | 125, 507 |
| BsaHI | GRCGYC | 3 cut(s) | 465, 473, 643 |
| BsaJI | CCNNGG | 4 cut(s) | 188, 227, 318, 350 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 428, 458 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 61, 173, 324, 351, 415 |
| Bse118I | RCCGGY | 3 cut(s) | 173, 193, 392 |
| Bse1I | ACTGG | 1 cut(s) | 438 |
| BseBI | CCWGG | 5 cut(s) | 189, 228, 319, 380, 452 |
| BseDI | CCNNGG | 4 cut(s) | 188, 227, 318, 350 |
| BseGI | GGATG | 1 cut(s) | 429 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 61, 173, 324, 351, 415 |
| BseMII | CTCAG | 1 cut(s) | 321 |
| BseNI | ACTGG | 1 cut(s) | 438 |
| BseXI | GCAGC | 1 cut(s) | 159 |
| BseYI | CCCAGC | 1 cut(s) | 408 |
| Bsh1236I | CGCG | 3 cut(s) | 71, 145, 524 |
| Bsh1285I | CGRYCG | 2 cut(s) | 121, 198 |
| BshFI | GGCC | 8 cut(s) | 47, 68, 113, 193, 374, 383, 510, 536 |
| BsiEI | CGRYCG | 2 cut(s) | 121, 198 |
| BsiSI | CCGG | 5 cut(s) | 125, 174, 194, 393, 507 |
| BslFI | GGGAC | 2 cut(s) | 258, 452 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 61, 173, 324, 351, 415 |
| BsmAI | GTCTC | 1 cut(s) | 452 |
| BsmBI | CGTCTC | 1 cut(s) | 452 |
| BsmFI | GGGAC | 2 cut(s) | 258, 452 |
| BsmI | GAATGC | 1 cut(s) | 319 |
| BsnI | GGCC | 8 cut(s) | 47, 68, 113, 193, 374, 383, 510, 536 |
| Bsp143I | GATC | 4 cut(s) | 337, 447, 525, 584 |
| BspANI | GGCC | 8 cut(s) | 47, 68, 113, 193, 374, 383, 510, 536 |
| BspCNI | CTCAG | 1 cut(s) | 322 |
| BspFNI | CGCG | 3 cut(s) | 71, 145, 524 |
| BspHI | TCATGA | 1 cut(s) | 75 |
| BspLI | GGNNCC | 3 cut(s) | 246, 485, 511 |
| BspMAI | CTGCAG | 1 cut(s) | 534 |
| BspPI | GGATC | 4 cut(s) | 332, 442, 520, 579 |
| BsrBI | CCGCTC | 1 cut(s) | 309 |
| BsrFI | RCCGGY | 3 cut(s) | 173, 193, 392 |
| BsrI | ACTGG | 1 cut(s) | 438 |
| BssAI | RCCGGY | 3 cut(s) | 173, 193, 392 |
| BssECI | CCNNGG | 4 cut(s) | 188, 227, 318, 350 |
| BssMI | GATC | 4 cut(s) | 337, 447, 525, 584 |
| BssNI | GRCGYC | 3 cut(s) | 465, 473, 643 |
| BssT1I | CCWWGG | 1 cut(s) | 350 |
| Bst2UI | CCWGG | 5 cut(s) | 189, 228, 319, 380, 452 |
| BstACI | GRCGYC | 3 cut(s) | 465, 473, 643 |
| BstC8I | GCNNGC | 7 cut(s) | 137, 175, 359, 372, 376, 394, 534 |
| BstDEI | CTNAG | 1 cut(s) | 330 |
| BstEII | GGTNACC | 1 cut(s) | 617 |
| BstF5I | GGATG | 1 cut(s) | 429 |
| BstFNI | CGCG | 3 cut(s) | 71, 145, 524 |
| BstHHI | GCGC | 4 cut(s) | 36, 73, 147, 357 |
| BstKTI | GATC | 4 cut(s) | 340, 450, 528, 587 |
| BstMAI | GTCTC | 1 cut(s) | 452 |
| BstMBI | GATC | 4 cut(s) | 337, 447, 525, 584 |
| BstMCI | CGRYCG | 2 cut(s) | 121, 198 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 214, 367, 380, 389, 398 |
| BstNI | CCWGG | 5 cut(s) | 189, 228, 319, 380, 452 |
| BstNSI | RCATGY | 1 cut(s) | 212 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 464 |
| BstPI | GGTNACC | 1 cut(s) | 617 |
| BstSCI | CCNGG | 7 cut(s) | 123, 187, 226, 317, 378, 450, 505 |
| BstSFI | CTRYAG | 1 cut(s) | 530 |
| BstUI | CGCG | 3 cut(s) | 71, 145, 524 |
| BstV1I | GCAGC | 1 cut(s) | 159 |
| BsuRI | GGCC | 8 cut(s) | 47, 68, 113, 193, 374, 383, 510, 536 |
| BtgZI | GCGATG | 1 cut(s) | 411 |
| BtsCI | GGATG | 1 cut(s) | 429 |
| Cac8I | GCNNGC | 7 cut(s) | 137, 175, 359, 372, 376, 394, 534 |
| CciI | TCATGA | 1 cut(s) | 75 |
| CfoI | GCGC | 4 cut(s) | 36, 73, 147, 357 |
| Cfr10I | RCCGGY | 3 cut(s) | 173, 193, 392 |
| Cfr13I | GGNCC | 3 cut(s) | 191, 245, 509 |
| CseI | GACGC | 2 cut(s) | 454, 651 |
| Csp6I | GTAC | 2 cut(s) | 200, 212 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 213, 248 |
| CviAII | CATG | 3 cut(s) | 76, 209, 599 |
| CviQI | GTAC | 2 cut(s) | 200, 212 |
| DdeI | CTNAG | 1 cut(s) | 330 |
| DpnI | GATC | 4 cut(s) | 339, 449, 527, 586 |
| DpnII | GATC | 4 cut(s) | 337, 447, 525, 584 |
| DraIII | CACNNNGTG | 1 cut(s) | 652 |
| EaeI | YGGCCR | 2 cut(s) | 66, 111 |
| EciI | GGCGGA | 5 cut(s) | 44, 330, 470, 508, 589 |
| Eco130I | CCWWGG | 1 cut(s) | 350 |
| Eco47I | GGWCC | 1 cut(s) | 245 |
| Eco91I | GGTNACC | 1 cut(s) | 617 |
| EcoO65I | GGTNACC | 1 cut(s) | 617 |
| EcoRII | CCWGG | 5 cut(s) | 187, 226, 317, 378, 450 |
| EcoT14I | CCWWGG | 1 cut(s) | 350 |
| EcoT22I | ATGCAT | 1 cut(s) | 210 |
| ErhI | CCWWGG | 1 cut(s) | 350 |
| Esp3I | CGTCTC | 1 cut(s) | 452 |
| FaeI | CATG | 3 cut(s) | 79, 212, 602 |
| FaiI | YATR | 9 cut(s) | 6, 77, 210, 257, 347, 542, 544, 571, 600 |
| FaqI | GGGAC | 2 cut(s) | 258, 452 |
| FatI | CATG | 3 cut(s) | 75, 208, 598 |
| FauNDI | CATATG | 1 cut(s) | 542 |
| FblI | GTMKAC | 1 cut(s) | 163 |
| Fnu4HI | GCNGC | 4 cut(s) | 69, 111, 148, 522 |
| FokI | GGATG | 1 cut(s) | 436 |
| Fsp4HI | GCNGC | 4 cut(s) | 69, 111, 148, 522 |
| FspBI | CTAG | 1 cut(s) | 351 |
| GlaI | GCGC | 4 cut(s) | 35, 72, 146, 356 |
| GluI | GCNGC | 4 cut(s) | 69, 111, 148, 522 |
| GsaI | CCCAGC | 1 cut(s) | 412 |
| GsuI | CTGGAG | 1 cut(s) | 455 |
| HaeIII | GGCC | 8 cut(s) | 47, 68, 113, 193, 374, 383, 510, 536 |
| HapII | CCGG | 5 cut(s) | 125, 174, 194, 393, 507 |
| HgaI | GACGC | 2 cut(s) | 454, 651 |
| HhaI | GCGC | 4 cut(s) | 36, 73, 147, 357 |
| Hin1I | GRCGYC | 3 cut(s) | 465, 473, 643 |
| Hin1II | CATG | 3 cut(s) | 79, 212, 602 |
| Hin6I | GCGC | 4 cut(s) | 34, 71, 145, 355 |
| HinP1I | GCGC | 4 cut(s) | 34, 71, 145, 355 |
| HincII | GTYRAC | 2 cut(s) | 164, 477 |
| HindII | GTYRAC | 2 cut(s) | 164, 477 |
| HinfI | GANTC | 3 cut(s) | 287, 386, 634 |
| HpaII | CCGG | 5 cut(s) | 125, 174, 194, 393, 507 |
| HphI | GGTGA | 2 cut(s) | 347, 611 |
| Hpy166II | GTNNAC | 4 cut(s) | 164, 477, 495, 596 |
| Hpy188I | TCNGA | 1 cut(s) | 483 |
| Hpy188III | TCNNGA | 5 cut(s) | 76, 237, 469, 501, 611 |
| Hpy8I | GTNNAC | 4 cut(s) | 164, 477, 495, 596 |
| Hpy99I | CGWCG | 3 cut(s) | 475, 642, 645 |
| HpyAV | CCTTC | 3 cut(s) | 37, 608, 617 |
| HpyCH4IV | ACGT | 2 cut(s) | 157, 473 |
| HpyCH4V | TGCA | 5 cut(s) | 208, 401, 427, 532, 546 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 214, 367, 380, 389, 398 |
| HpyF3I | CTNAG | 1 cut(s) | 330 |
| HpySE526I | ACGT | 2 cut(s) | 157, 473 |
| Hsp92I | GRCGYC | 3 cut(s) | 465, 473, 643 |
| Hsp92II | CATG | 3 cut(s) | 79, 212, 602 |
| HspAI | GCGC | 4 cut(s) | 34, 71, 145, 355 |
| KroI | GCCGGC | 2 cut(s) | 173, 392 |
| KroNI | GCCGGC | 2 cut(s) | 175, 394 |
| Kzo9I | GATC | 4 cut(s) | 337, 447, 525, 584 |
| LmnI | GCTCC | 2 cut(s) | 31, 436 |
| Lsp1109I | GCAGC | 1 cut(s) | 159 |
| LweI | GCATC | 3 cut(s) | 174, 388, 414 |
| MaeI | CTAG | 1 cut(s) | 351 |
| MaeII | ACGT | 2 cut(s) | 157, 473 |
| MaeIII | GTNAC | 1 cut(s) | 617 |
| MalI | GATC | 4 cut(s) | 339, 449, 527, 586 |
| MbiI | CCGCTC | 1 cut(s) | 309 |
| MboI | GATC | 4 cut(s) | 337, 447, 525, 584 |
| MboII | GAAGA | 1 cut(s) | 296 |
| MnlI | CCTC | 5 cut(s) | 19, 82, 303, 325, 435 |
| Mph1103I | ATGCAT | 1 cut(s) | 210 |
| MroNI | GCCGGC | 2 cut(s) | 173, 392 |
| MslI | CAYNNNNRTG | 1 cut(s) | 182 |
| MspA1I | CMGCKG | 2 cut(s) | 217, 490 |
| MspI | CCGG | 5 cut(s) | 125, 174, 194, 393, 507 |
| MspR9I | CCNGG | 7 cut(s) | 125, 189, 228, 319, 380, 452, 507 |
| Mva1269I | GAATGC | 1 cut(s) | 319 |
| MvaI | CCWGG | 5 cut(s) | 189, 228, 319, 380, 452 |
| MvnI | CGCG | 3 cut(s) | 71, 145, 524 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 214, 367, 380, 389, 398 |
| NaeI | GCCGGC | 2 cut(s) | 175, 394 |
| NciI | CCSGG | 2 cut(s) | 125, 507 |
| NdeI | CATATG | 1 cut(s) | 542 |
| NdeII | GATC | 4 cut(s) | 337, 447, 525, 584 |
| NgoMIV | GCCGGC | 2 cut(s) | 173, 392 |
| NlaIII | CATG | 3 cut(s) | 79, 212, 602 |
| NlaIV | GGNNCC | 3 cut(s) | 246, 485, 511 |
| NmeAIII | GCCGAG | 1 cut(s) | 346 |
| NmuCI | GTSAC | 1 cut(s) | 617 |
| NsiI | ATGCAT | 1 cut(s) | 210 |
| NspI | RCATGY | 1 cut(s) | 212 |
| PagI | TCATGA | 1 cut(s) | 75 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 467 |
| PctI | GAATGC | 1 cut(s) | 319 |
| PdiI | GCCGGC | 2 cut(s) | 175, 394 |
| PfeI | GAWTC | 3 cut(s) | 287, 386, 634 |
| PflFI | GACNNNGTC | 1 cut(s) | 159 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 415 |
| PkrI | GCNGC | 4 cut(s) | 70, 112, 149, 523 |
| PshAI | GACNNNNGTC | 1 cut(s) | 464 |
| Psp6I | CCWGG | 5 cut(s) | 187, 226, 317, 378, 450 |
| PspEI | GGTNACC | 1 cut(s) | 617 |
| PspFI | CCCAGC | 1 cut(s) | 408 |
| PspGI | CCWGG | 5 cut(s) | 187, 226, 317, 378, 450 |
| PspN4I | GGNNCC | 3 cut(s) | 246, 485, 511 |
| PspPI | GGNCC | 3 cut(s) | 191, 245, 509 |
| PstI | CTGCAG | 1 cut(s) | 534 |
| PsyI | GACNNNGTC | 1 cut(s) | 159 |
| PvuII | CAGCTG | 1 cut(s) | 490 |
| RsaI | GTAC | 2 cut(s) | 201, 213 |
| RsaNI | GTAC | 2 cut(s) | 200, 212 |
| RseI | CAYNNNNRTG | 1 cut(s) | 182 |
| SalI | GTCGAC | 1 cut(s) | 162 |
| SatI | GCNGC | 4 cut(s) | 69, 111, 148, 522 |
| Sau3AI | GATC | 4 cut(s) | 337, 447, 525, 584 |
| Sau96I | GGNCC | 3 cut(s) | 191, 245, 509 |
| SbfI | CCTGCAGG | 1 cut(s) | 534 |
| ScrFI | CCNGG | 7 cut(s) | 125, 189, 228, 319, 380, 452, 507 |
| SdaI | CCTGCAGG | 1 cut(s) | 534 |
| SfaNI | GCATC | 3 cut(s) | 174, 388, 414 |
| SfcI | CTRYAG | 1 cut(s) | 530 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 380 |
| SinI | GGWCC | 1 cut(s) | 245 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 182 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 534 |
| SspMI | CTAG | 1 cut(s) | 351 |
| StyD4I | CCNGG | 7 cut(s) | 123, 187, 226, 317, 378, 450, 505 |
| StyI | CCWWGG | 1 cut(s) | 350 |
| TaiI | ACGT | 2 cut(s) | 160, 476 |
| TaqI | TCGA | 3 cut(s) | 163, 311, 637 |
| TauI | GCSGC | 3 cut(s) | 71, 113, 524 |
| TfiI | GAWTC | 3 cut(s) | 287, 386, 634 |
| TseFI | GTSAC | 1 cut(s) | 617 |
| TseI | GCWGC | 1 cut(s) | 147 |
| Tsp45I | GTSAC | 1 cut(s) | 617 |
| Tth111I | GACNNNGTC | 1 cut(s) | 159 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 415 |
| VpaK11BI | GGWCC | 1 cut(s) | 245 |
| XceI | RCATGY | 1 cut(s) | 212 |
| XmaJI | CCTAGG | 1 cut(s) | 350 |
| XmiI | GTMKAC | 1 cut(s) | 163 |
| XspI | CTAG | 1 cut(s) | 351 |
| ZraI | GACGTC | 1 cut(s) | 474 |
| Zsp2I | ATGCAT | 1 cut(s) | 210 |