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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 240 |
| Acc65I | GGTACC | 1 cut(s) | 133 |
| AccB1I | GGYRCC | 1 cut(s) | 133 |
| AccI | GTMKAC | 2 cut(s) | 112, 419 |
| AciI | CCGC | 3 cut(s) | 204, 485, 515 |
| AclWI | GGATC | 1 cut(s) | 347 |
| AcsI | RAATTY | 1 cut(s) | 603 |
| AfaI | GTAC | 1 cut(s) | 135 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 562 |
| AgsI | TTSAA | 2 cut(s) | 451, 461 |
| AluBI | AGCT | 3 cut(s) | 177, 221, 284 |
| AluI | AGCT | 3 cut(s) | 177, 221, 284 |
| Alw26I | GTCTC | 1 cut(s) | 189 |
| AlwI | GGATC | 1 cut(s) | 347 |
| Ama87I | CYCGRG | 1 cut(s) | 128 |
| AoxI | GGCC | 2 cut(s) | 357, 553 |
| ApeKI | GCWGC | 1 cut(s) | 413 |
| ApoI | RAATTY | 1 cut(s) | 603 |
| Asp718I | GGTACC | 1 cut(s) | 133 |
| AspLEI | GCGC | 1 cut(s) | 586 |
| AspS9I | GGNCC | 2 cut(s) | 229, 358 |
| AsuC2I | CCSGG | 1 cut(s) | 168 |
| AsuHPI | GGTGA | 1 cut(s) | 81 |
| AvaI | CYCGRG | 1 cut(s) | 128 |
| AvaII | GGWCC | 1 cut(s) | 229 |
| BanI | GGYRCC | 1 cut(s) | 133 |
| BanII | GRGCYC | 1 cut(s) | 597 |
| BbvI | GCAGC | 1 cut(s) | 425 |
| BccI | CCATC | 1 cut(s) | 101 |
| BcnI | CCSGG | 1 cut(s) | 168 |
| BcoDI | GTCTC | 1 cut(s) | 189 |
| BfaI | CTAG | 4 cut(s) | 15, 174, 218, 338 |
| BfoI | RGCGCY | 1 cut(s) | 587 |
| BfuAI | ACCTGC | 1 cut(s) | 240 |
| BisI | GCNGC | 2 cut(s) | 204, 414 |
| BlpI | GCTNAGC | 1 cut(s) | 389 |
| BlsI | GCNGC | 2 cut(s) | 205, 415 |
| Bme1390I | CCNGG | 1 cut(s) | 168 |
| Bme18I | GGWCC | 1 cut(s) | 229 |
| BmeT110I | CYCGRG | 1 cut(s) | 128 |
| BmgT120I | GGNCC | 2 cut(s) | 229, 358 |
| BmiI | GGNNCC | 1 cut(s) | 135 |
| BmrFI | CCNGG | 1 cut(s) | 168 |
| BpmI | CTGGAG | 1 cut(s) | 485 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 389 |
| BpuMI | CCSGG | 1 cut(s) | 168 |
| BsaI | GGTCTC | 1 cut(s) | 189 |
| BsaJI | CCNNGG | 1 cut(s) | 129 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 562 |
| Bse1I | ACTGG | 1 cut(s) | 468 |
| BseDI | CCNNGG | 1 cut(s) | 129 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 562 |
| BseNI | ACTGG | 1 cut(s) | 468 |
| BseXI | GCAGC | 1 cut(s) | 425 |
| BsgI | GTGCAG | 2 cut(s) | 218, 521 |
| BshFI | GGCC | 2 cut(s) | 359, 555 |
| BshNI | GGYRCC | 1 cut(s) | 133 |
| BsiHKCI | CYCGRG | 1 cut(s) | 128 |
| BsiSI | CCGG | 2 cut(s) | 168, 356 |
| BslFI | GGGAC | 1 cut(s) | 355 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 562 |
| BsmAI | GTCTC | 1 cut(s) | 189 |
| BsmFI | GGGAC | 1 cut(s) | 355 |
| BsnI | GGCC | 2 cut(s) | 359, 555 |
| Bso31I | GGTCTC | 1 cut(s) | 189 |
| BsoBI | CYCGRG | 1 cut(s) | 128 |
| Bsp1286I | GDGCHC | 1 cut(s) | 597 |
| Bsp143I | GATC | 2 cut(s) | 96, 352 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 389 |
| BspACI | CCGC | 3 cut(s) | 204, 485, 515 |
| BspANI | GGCC | 2 cut(s) | 359, 555 |
| BspLI | GGNNCC | 1 cut(s) | 135 |
| BspMI | ACCTGC | 1 cut(s) | 240 |
| BspPI | GGATC | 1 cut(s) | 347 |
| BspT107I | GGYRCC | 1 cut(s) | 133 |
| BspTNI | GGTCTC | 1 cut(s) | 189 |
| BsrI | ACTGG | 1 cut(s) | 468 |
| BssECI | CCNNGG | 1 cut(s) | 129 |
| BssMI | GATC | 2 cut(s) | 96, 352 |
| Bst4CI | ACNGT | 1 cut(s) | 525 |
| BstC8I | GCNNGC | 3 cut(s) | 480, 557, 582 |
| BstDEI | CTNAG | 1 cut(s) | 389 |
| BstH2I | RGCGCY | 1 cut(s) | 587 |
| BstHHI | GCGC | 1 cut(s) | 586 |
| BstKTI | GATC | 2 cut(s) | 99, 355 |
| BstMAI | GTCTC | 1 cut(s) | 189 |
| BstMBI | GATC | 2 cut(s) | 96, 352 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 356, 413, 484, 592 |
| BstNSI | RCATGY | 2 cut(s) | 512, 580 |
| BstSCI | CCNGG | 1 cut(s) | 166 |
| BstV1I | GCAGC | 1 cut(s) | 425 |
| BstX2I | RGATCY | 1 cut(s) | 352 |
| BstYI | RGATCY | 1 cut(s) | 352 |
| BsuRI | GGCC | 2 cut(s) | 359, 555 |
| BtsIMutI | CAGTG | 1 cut(s) | 102 |
| BveI | ACCTGC | 1 cut(s) | 240 |
| Cac8I | GCNNGC | 3 cut(s) | 480, 557, 582 |
| CfoI | GCGC | 1 cut(s) | 586 |
| Cfr13I | GGNCC | 2 cut(s) | 229, 358 |
| CseI | GACGC | 1 cut(s) | 405 |
| Csp6I | GTAC | 1 cut(s) | 134 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 477, 512 |
| CviAII | CATG | 3 cut(s) | 208, 509, 577 |
| CviJI | RGCY | 9 cut(s) | 177, 221, 284, 359, 388, 393, 478, 555, 595 |
| CviKI_1 | RGCY | 9 cut(s) | 177, 221, 284, 359, 388, 393, 478, 555, 595 |
| CviQI | GTAC | 1 cut(s) | 134 |
| DdeI | CTNAG | 1 cut(s) | 389 |
| DpnI | GATC | 2 cut(s) | 98, 354 |
| DpnII | GATC | 2 cut(s) | 96, 352 |
| Eco24I | GRGCYC | 1 cut(s) | 597 |
| Eco31I | GGTCTC | 1 cut(s) | 189 |
| Eco47I | GGWCC | 1 cut(s) | 229 |
| Eco88I | CYCGRG | 1 cut(s) | 128 |
| EcoT38I | GRGCYC | 1 cut(s) | 597 |
| FaeI | CATG | 3 cut(s) | 211, 512, 580 |
| FaiI | YATR | 5 cut(s) | 209, 439, 510, 534, 578 |
| FaqI | GGGAC | 1 cut(s) | 355 |
| FatI | CATG | 3 cut(s) | 207, 508, 576 |
| FblI | GTMKAC | 2 cut(s) | 112, 419 |
| Fnu4HI | GCNGC | 2 cut(s) | 204, 414 |
| FriOI | GRGCYC | 1 cut(s) | 597 |
| Fsp4HI | GCNGC | 2 cut(s) | 204, 414 |
| FspBI | CTAG | 4 cut(s) | 15, 174, 218, 338 |
| GlaI | GCGC | 1 cut(s) | 585 |
| GluI | GCNGC | 2 cut(s) | 204, 414 |
| GsuI | CTGGAG | 1 cut(s) | 485 |
| HaeII | RGCGCY | 1 cut(s) | 587 |
| HaeIII | GGCC | 2 cut(s) | 359, 555 |
| HapII | CCGG | 2 cut(s) | 168, 356 |
| HgaI | GACGC | 1 cut(s) | 405 |
| HhaI | GCGC | 1 cut(s) | 586 |
| Hin1II | CATG | 3 cut(s) | 211, 512, 580 |
| Hin6I | GCGC | 1 cut(s) | 584 |
| HinP1I | GCGC | 1 cut(s) | 584 |
| HincII | GTYRAC | 4 cut(s) | 9, 33, 113, 420 |
| HindII | GTYRAC | 4 cut(s) | 9, 33, 113, 420 |
| HinfI | GANTC | 1 cut(s) | 518 |
| HpaII | CCGG | 2 cut(s) | 168, 356 |
| HphI | GGTGA | 1 cut(s) | 81 |
| Hpy166II | GTNNAC | 6 cut(s) | 9, 33, 89, 113, 229, 420 |
| Hpy188III | TCNNGA | 2 cut(s) | 79, 194 |
| Hpy8I | GTNNAC | 6 cut(s) | 9, 33, 89, 113, 229, 420 |
| Hpy99I | CGWCG | 1 cut(s) | 421 |
| HpyAV | CCTTC | 2 cut(s) | 268, 325 |
| HpyCH4III | ACNGT | 1 cut(s) | 525 |
| HpyCH4V | TGCA | 6 cut(s) | 235, 350, 376, 383, 502, 529 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 356, 413, 484, 592 |
| HpyF3I | CTNAG | 1 cut(s) | 389 |
| Hsp92II | CATG | 3 cut(s) | 211, 512, 580 |
| HspAI | GCGC | 1 cut(s) | 584 |
| KpnI | GGTACC | 1 cut(s) | 137 |
| Kzo9I | GATC | 2 cut(s) | 96, 352 |
| LmnI | GCTCC | 2 cut(s) | 262, 505 |
| Lsp1109I | GCAGC | 1 cut(s) | 425 |
| MaeI | CTAG | 4 cut(s) | 15, 174, 218, 338 |
| MalI | GATC | 2 cut(s) | 98, 354 |
| MboI | GATC | 2 cut(s) | 96, 352 |
| MfeI | CAATTG | 1 cut(s) | 494 |
| MflI | RGATCY | 1 cut(s) | 352 |
| MhlI | GDGCHC | 1 cut(s) | 597 |
| MluCI | AATT | 3 cut(s) | 82, 494, 603 |
| MlyI | GAGTC | 1 cut(s) | 512 |
| MmeI | TCCRAC | 1 cut(s) | 85 |
| MnlI | CCTC | 5 cut(s) | 47, 124, 209, 460, 555 |
| MseI | TTAA | 3 cut(s) | 120, 318, 613 |
| MspI | CCGG | 2 cut(s) | 168, 356 |
| MspR9I | CCNGG | 1 cut(s) | 168 |
| MunI | CAATTG | 1 cut(s) | 494 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 356, 413, 484, 592 |
| NciI | CCSGG | 1 cut(s) | 168 |
| NdeII | GATC | 2 cut(s) | 96, 352 |
| NlaIII | CATG | 3 cut(s) | 211, 512, 580 |
| NlaIV | GGNNCC | 1 cut(s) | 135 |
| NspI | RCATGY | 2 cut(s) | 512, 580 |
| PkrI | GCNGC | 2 cut(s) | 205, 415 |
| PleI | GAGTC | 1 cut(s) | 512 |
| PpsI | GAGTC | 1 cut(s) | 512 |
| PspN4I | GGNNCC | 1 cut(s) | 135 |
| PspPI | GGNCC | 2 cut(s) | 229, 358 |
| PsuI | RGATCY | 1 cut(s) | 352 |
| RsaI | GTAC | 1 cut(s) | 135 |
| RsaNI | GTAC | 1 cut(s) | 134 |
| SalI | GTCGAC | 2 cut(s) | 111, 418 |
| SaqAI | TTAA | 3 cut(s) | 120, 318, 613 |
| SatI | GCNGC | 2 cut(s) | 204, 414 |
| Sau3AI | GATC | 2 cut(s) | 96, 352 |
| Sau96I | GGNCC | 2 cut(s) | 229, 358 |
| SchI | GAGTC | 1 cut(s) | 512 |
| ScrFI | CCNGG | 1 cut(s) | 168 |
| SduI | GDGCHC | 1 cut(s) | 597 |
| SetI | ASST | 6 cut(s) | 135, 179, 201, 223, 234, 286 |
| SinI | GGWCC | 1 cut(s) | 229 |
| Sse9I | AATT | 3 cut(s) | 82, 494, 603 |
| SsiI | CCGC | 3 cut(s) | 204, 485, 515 |
| SspMI | CTAG | 4 cut(s) | 15, 174, 218, 338 |
| StyD4I | CCNGG | 1 cut(s) | 166 |
| TaaI | ACNGT | 1 cut(s) | 525 |
| TaqI | TCGA | 3 cut(s) | 112, 193, 419 |
| TasI | AATT | 3 cut(s) | 82, 494, 603 |
| TauI | GCSGC | 1 cut(s) | 206 |
| Tru1I | TTAA | 3 cut(s) | 120, 318, 613 |
| Tru9I | TTAA | 3 cut(s) | 120, 318, 613 |
| TscAI | CASTG | 1 cut(s) | 109 |
| TseI | GCWGC | 1 cut(s) | 413 |
| TspGWI | ACGGA | 1 cut(s) | 139 |
| TspRI | CASTG | 1 cut(s) | 109 |
| VpaK11BI | GGWCC | 1 cut(s) | 229 |
| XapI | RAATTY | 1 cut(s) | 603 |
| XceI | RCATGY | 2 cut(s) | 512, 580 |
| XmiI | GTMKAC | 2 cut(s) | 112, 419 |
| XspI | CTAG | 4 cut(s) | 15, 174, 218, 338 |