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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 322 |
| Acc65I | GGTACC | 2 cut(s) | 307, 334 |
| AccB1I | GGYRCC | 2 cut(s) | 307, 334 |
| AccI | GTMKAC | 2 cut(s) | 47, 286 |
| AciI | CCGC | 4 cut(s) | 119, 312, 338, 347 |
| AclWI | GGATC | 2 cut(s) | 53, 96 |
| AcoI | YGGCCR | 1 cut(s) | 189 |
| AcsI | RAATTY | 1 cut(s) | 206 |
| AcuI | CTGAAG | 1 cut(s) | 239 |
| AfaI | GTAC | 2 cut(s) | 309, 336 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 130, 296 |
| AgeI | ACCGGT | 1 cut(s) | 296 |
| AluBI | AGCT | 1 cut(s) | 145 |
| AluI | AGCT | 1 cut(s) | 145 |
| Alw21I | GWGCWC | 2 cut(s) | 147, 275 |
| AlwI | GGATC | 2 cut(s) | 53, 96 |
| Ama87I | CYCGRG | 2 cut(s) | 146, 350 |
| AoxI | GGCC | 4 cut(s) | 31, 168, 174, 189 |
| ApoI | RAATTY | 1 cut(s) | 206 |
| AsiGI | ACCGGT | 1 cut(s) | 296 |
| Asp718I | GGTACC | 2 cut(s) | 307, 334 |
| AspLEI | GCGC | 3 cut(s) | 159, 229, 253 |
| AspS9I | GGNCC | 4 cut(s) | 31, 121, 175, 368 |
| AsuC2I | CCSGG | 1 cut(s) | 83 |
| AvaI | CYCGRG | 2 cut(s) | 146, 350 |
| AvaII | GGWCC | 2 cut(s) | 121, 368 |
| BalI | TGGCCA | 1 cut(s) | 191 |
| BanI | GGYRCC | 2 cut(s) | 307, 334 |
| BanII | GRGCYC | 1 cut(s) | 147 |
| Bbv12I | GWGCWC | 2 cut(s) | 147, 275 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BcnI | CCSGG | 1 cut(s) | 83 |
| BfaI | CTAG | 1 cut(s) | 395 |
| BfoI | RGCGCY | 1 cut(s) | 230 |
| BfuAI | ACCTGC | 1 cut(s) | 322 |
| BlpI | GCTNAGC | 1 cut(s) | 269 |
| Bme1390I | CCNGG | 1 cut(s) | 83 |
| Bme18I | GGWCC | 2 cut(s) | 121, 368 |
| BmeT110I | CYCGRG | 2 cut(s) | 146, 350 |
| BmgT120I | GGNCC | 4 cut(s) | 31, 121, 175, 368 |
| BmiI | GGNNCC | 3 cut(s) | 309, 336, 369 |
| BmrFI | CCNGG | 1 cut(s) | 83 |
| BpmI | CTGGAG | 1 cut(s) | 251 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 269 |
| BpuMI | CCSGG | 1 cut(s) | 83 |
| BsaJI | CCNNGG | 1 cut(s) | 171 |
| BsaWI | WCCGGW | 1 cut(s) | 296 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 130, 296 |
| Bse118I | RCCGGY | 1 cut(s) | 296 |
| BseDI | CCNNGG | 1 cut(s) | 171 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 130, 296 |
| BseMII | CTCAG | 2 cut(s) | 18, 260 |
| BseYI | CCCAGC | 1 cut(s) | 247 |
| BshFI | GGCC | 4 cut(s) | 33, 170, 176, 191 |
| BshNI | GGYRCC | 2 cut(s) | 307, 334 |
| BshTI | ACCGGT | 1 cut(s) | 296 |
| BsiHKAI | GWGCWC | 2 cut(s) | 147, 275 |
| BsiHKCI | CYCGRG | 2 cut(s) | 146, 350 |
| BsiSI | CCGG | 2 cut(s) | 82, 297 |
| BslFI | GGGAC | 1 cut(s) | 381 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 130, 296 |
| BsmFI | GGGAC | 1 cut(s) | 381 |
| BsnI | GGCC | 4 cut(s) | 33, 170, 176, 191 |
| BsoBI | CYCGRG | 2 cut(s) | 146, 350 |
| Bsp1286I | GDGCHC | 2 cut(s) | 147, 275 |
| Bsp143I | GATC | 3 cut(s) | 40, 58, 88 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 269 |
| BspACI | CCGC | 4 cut(s) | 119, 312, 338, 347 |
| BspANI | GGCC | 4 cut(s) | 33, 170, 176, 191 |
| BspCNI | CTCAG | 2 cut(s) | 19, 261 |
| BspLI | GGNNCC | 3 cut(s) | 309, 336, 369 |
| BspMI | ACCTGC | 1 cut(s) | 322 |
| BspPI | GGATC | 2 cut(s) | 53, 96 |
| BspT107I | GGYRCC | 2 cut(s) | 307, 334 |
| BsrFI | RCCGGY | 1 cut(s) | 296 |
| BssAI | RCCGGY | 1 cut(s) | 296 |
| BssECI | CCNNGG | 1 cut(s) | 171 |
| BssMI | GATC | 3 cut(s) | 40, 58, 88 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 1 cut(s) | 260 |
| BstDEI | CTNAG | 2 cut(s) | 27, 269 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 128 |
| BstH2I | RGCGCY | 1 cut(s) | 230 |
| BstHHI | GCGC | 3 cut(s) | 159, 229, 253 |
| BstKTI | GATC | 3 cut(s) | 43, 61, 91 |
| BstMBI | GATC | 3 cut(s) | 40, 58, 88 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 233, 259, 337, 346 |
| BstNSI | RCATGY | 1 cut(s) | 188 |
| BstSCI | CCNGG | 1 cut(s) | 81 |
| BstX2I | RGATCY | 1 cut(s) | 88 |
| BstYI | RGATCY | 1 cut(s) | 88 |
| BsuRI | GGCC | 4 cut(s) | 33, 170, 176, 191 |
| BtsI | GCAGTG | 1 cut(s) | 93 |
| BtsIMutI | CAGTG | 1 cut(s) | 93 |
| BveI | ACCTGC | 1 cut(s) | 322 |
| Cac8I | GCNNGC | 1 cut(s) | 260 |
| CfoI | GCGC | 3 cut(s) | 159, 229, 253 |
| Cfr10I | RCCGGY | 1 cut(s) | 296 |
| Cfr13I | GGNCC | 4 cut(s) | 31, 121, 175, 368 |
| Csp6I | GTAC | 2 cut(s) | 308, 335 |
| CspAI | ACCGGT | 1 cut(s) | 296 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 52, 87, 280, 315 |
| CviAII | CATG | 2 cut(s) | 185, 193 |
| CviJI | RGCY | 5 cut(s) | 33, 145, 170, 176, 191 |
| CviKI_1 | RGCY | 5 cut(s) | 33, 145, 170, 176, 191 |
| CviQI | GTAC | 2 cut(s) | 308, 335 |
| DdeI | CTNAG | 2 cut(s) | 27, 269 |
| DpnI | GATC | 3 cut(s) | 42, 60, 90 |
| DpnII | GATC | 3 cut(s) | 40, 58, 88 |
| EaeI | YGGCCR | 1 cut(s) | 189 |
| EciI | GGCGGA | 1 cut(s) | 134 |
| Ecl136II | GAGCTC | 1 cut(s) | 145 |
| Eco24I | GRGCYC | 1 cut(s) | 147 |
| Eco47I | GGWCC | 2 cut(s) | 121, 368 |
| Eco53kI | GAGCTC | 1 cut(s) | 145 |
| Eco57I | CTGAAG | 1 cut(s) | 239 |
| Eco88I | CYCGRG | 2 cut(s) | 146, 350 |
| EcoICRI | GAGCTC | 1 cut(s) | 145 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 128 |
| EcoT38I | GRGCYC | 1 cut(s) | 147 |
| FaeI | CATG | 2 cut(s) | 188, 196 |
| FaiI | YATR | 6 cut(s) | 105, 186, 194, 323, 327, 329 |
| FaqI | GGGAC | 1 cut(s) | 381 |
| FatI | CATG | 2 cut(s) | 184, 192 |
| FauI | CCCGC | 2 cut(s) | 319, 354 |
| FauNDI | CATATG | 1 cut(s) | 327 |
| FblI | GTMKAC | 2 cut(s) | 47, 286 |
| FriOI | GRGCYC | 1 cut(s) | 147 |
| FspBI | CTAG | 1 cut(s) | 395 |
| GlaI | GCGC | 3 cut(s) | 158, 228, 252 |
| GsaI | CCCAGC | 1 cut(s) | 251 |
| GsuI | CTGGAG | 1 cut(s) | 251 |
| HaeII | RGCGCY | 1 cut(s) | 230 |
| HaeIII | GGCC | 4 cut(s) | 33, 170, 176, 191 |
| HapII | CCGG | 2 cut(s) | 82, 297 |
| HhaI | GCGC | 3 cut(s) | 159, 229, 253 |
| Hin1II | CATG | 2 cut(s) | 188, 196 |
| Hin6I | GCGC | 3 cut(s) | 157, 227, 251 |
| HinP1I | GCGC | 3 cut(s) | 157, 227, 251 |
| HincII | GTYRAC | 1 cut(s) | 287 |
| HindII | GTYRAC | 1 cut(s) | 287 |
| HpaII | CCGG | 2 cut(s) | 82, 297 |
| Hpy166II | GTNNAC | 2 cut(s) | 48, 287 |
| Hpy188III | TCNNGA | 1 cut(s) | 56 |
| Hpy8I | GTNNAC | 2 cut(s) | 48, 287 |
| HpyAV | CCTTC | 1 cut(s) | 217 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 280 |
| HpyCH4V | TGCA | 1 cut(s) | 331 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 233, 259, 337, 346 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 269 |
| HpySE526I | ACGT | 1 cut(s) | 280 |
| Hsp92II | CATG | 2 cut(s) | 188, 196 |
| HspAI | GCGC | 3 cut(s) | 157, 227, 251 |
| KpnI | GGTACC | 2 cut(s) | 311, 338 |
| Kzo9I | GATC | 3 cut(s) | 40, 58, 88 |
| LmnI | GCTCC | 1 cut(s) | 142 |
| LpnPI | CCDG | 7 cut(s) | 95, 124, 215, 261, 289, 310, 317 |
| MaeI | CTAG | 1 cut(s) | 395 |
| MaeII | ACGT | 1 cut(s) | 280 |
| MalI | GATC | 3 cut(s) | 42, 60, 90 |
| MboI | GATC | 3 cut(s) | 40, 58, 88 |
| MboII | GAAGA | 1 cut(s) | 232 |
| MflI | RGATCY | 1 cut(s) | 88 |
| MhlI | GDGCHC | 2 cut(s) | 147, 275 |
| MlsI | TGGCCA | 1 cut(s) | 191 |
| MluCI | AATT | 1 cut(s) | 206 |
| MluNI | TGGCCA | 1 cut(s) | 191 |
| MnlI | CCTC | 6 cut(s) | 22, 79, 134, 166, 226, 384 |
| Mox20I | TGGCCA | 1 cut(s) | 191 |
| MscI | TGGCCA | 1 cut(s) | 191 |
| MslI | CAYNNNNRTG | 2 cut(s) | 197, 326 |
| Msp20I | TGGCCA | 1 cut(s) | 191 |
| MspA1I | CMGCKG | 1 cut(s) | 340 |
| MspI | CCGG | 2 cut(s) | 82, 297 |
| MspR9I | CCNGG | 1 cut(s) | 83 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 233, 259, 337, 346 |
| NciI | CCSGG | 1 cut(s) | 83 |
| NdeI | CATATG | 1 cut(s) | 327 |
| NdeII | GATC | 3 cut(s) | 40, 58, 88 |
| NlaIII | CATG | 2 cut(s) | 188, 196 |
| NlaIV | GGNNCC | 3 cut(s) | 309, 336, 369 |
| NmeAIII | GCCGAG | 1 cut(s) | 196 |
| NspI | RCATGY | 1 cut(s) | 188 |
| PaeR7I | CTCGAG | 1 cut(s) | 146 |
| PflFI | GACNNNGTC | 1 cut(s) | 282 |
| PinAI | ACCGGT | 1 cut(s) | 296 |
| Psp124BI | GAGCTC | 1 cut(s) | 147 |
| PspFI | CCCAGC | 1 cut(s) | 247 |
| PspN4I | GGNNCC | 3 cut(s) | 309, 336, 369 |
| PspPI | GGNCC | 4 cut(s) | 31, 121, 175, 368 |
| PspXI | VCTCGAGB | 1 cut(s) | 146 |
| PsuI | RGATCY | 1 cut(s) | 88 |
| PsyI | GACNNNGTC | 1 cut(s) | 282 |
| RsaI | GTAC | 2 cut(s) | 309, 336 |
| RsaNI | GTAC | 2 cut(s) | 308, 335 |
| RseI | CAYNNNNRTG | 2 cut(s) | 197, 326 |
| SacI | GAGCTC | 1 cut(s) | 147 |
| SalI | GTCGAC | 1 cut(s) | 285 |
| Sau3AI | GATC | 3 cut(s) | 40, 58, 88 |
| Sau96I | GGNCC | 4 cut(s) | 31, 121, 175, 368 |
| ScrFI | CCNGG | 1 cut(s) | 83 |
| SduI | GDGCHC | 2 cut(s) | 147, 275 |
| SetI | ASST | 6 cut(s) | 72, 126, 147, 283, 336, 376 |
| Sfr274I | CTCGAG | 1 cut(s) | 146 |
| SinI | GGWCC | 2 cut(s) | 121, 368 |
| SlaI | CTCGAG | 1 cut(s) | 146 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 197, 326 |
| SmlI | CTYRAG | 1 cut(s) | 146 |
| SmoI | CTYRAG | 1 cut(s) | 146 |
| Sse9I | AATT | 1 cut(s) | 206 |
| SsiI | CCGC | 4 cut(s) | 119, 312, 338, 347 |
| SspMI | CTAG | 1 cut(s) | 395 |
| SstI | GAGCTC | 1 cut(s) | 147 |
| StyD4I | CCNGG | 1 cut(s) | 81 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 283 |
| TaqI | TCGA | 4 cut(s) | 57, 147, 210, 286 |
| TasI | AATT | 1 cut(s) | 206 |
| TscAI | CASTG | 1 cut(s) | 100 |
| TspDTI | ATGAA | 1 cut(s) | 228 |
| TspRI | CASTG | 1 cut(s) | 100 |
| Tth111I | GACNNNGTC | 1 cut(s) | 282 |
| VpaK11BI | GGWCC | 2 cut(s) | 121, 368 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 128 |
| XapI | RAATTY | 1 cut(s) | 206 |
| XceI | RCATGY | 1 cut(s) | 188 |
| XhoI | CTCGAG | 1 cut(s) | 146 |
| XmiI | GTMKAC | 2 cut(s) | 47, 286 |
| XspI | CTAG | 1 cut(s) | 395 |