Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1141 |
| Acc36I | ACCTGC | 1 cut(s) | 1141 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 846 |
| AccI | GTMKAC | 1 cut(s) | 1249 |
| AccII | CGCG | 3 cut(s) | 802, 1030, 1234 |
| AciI | CCGC | 8 cut(s) | 92, 611, 936, 945, 1028, 1062, 1085, 1234 |
| AclWI | GGATC | 2 cut(s) | 41, 437 |
| AcoI | YGGCCR | 1 cut(s) | 817 |
| AcsI | RAATTY | 2 cut(s) | 396, 1172 |
| AcuI | CTGAAG | 1 cut(s) | 977 |
| AfaI | GTAC | 1 cut(s) | 411 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 98, 846, 914, 1096, 1150, 1262 |
| AgsI | TTSAA | 4 cut(s) | 76, 162, 436, 1177 |
| AjiI | CACGTC | 1 cut(s) | 715 |
| AjnI | CCWGG | 4 cut(s) | 495, 773, 1045, 1050 |
| AluBI | AGCT | 8 cut(s) | 339, 369, 492, 641, 814, 837, 1075, 1162 |
| AluI | AGCT | 8 cut(s) | 339, 369, 492, 641, 814, 837, 1075, 1162 |
| Alw21I | GWGCWC | 2 cut(s) | 463, 1241 |
| Alw26I | GTCTC | 1 cut(s) | 953 |
| Alw44I | GTGCAC | 1 cut(s) | 459 |
| AlwI | GGATC | 2 cut(s) | 41, 437 |
| AoxI | GGCC | 5 cut(s) | 817, 1020, 1048, 1134, 1140 |
| ApaLI | GTGCAC | 1 cut(s) | 459 |
| ApeKI | GCWGC | 2 cut(s) | 1013, 1032 |
| ApoI | RAATTY | 2 cut(s) | 396, 1172 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1151, 1183 |
| AspLEI | GCGC | 6 cut(s) | 233, 741, 804, 1032, 1195, 1219 |
| AspS9I | GGNCC | 4 cut(s) | 25, 1021, 1087, 1141 |
| AsuHPI | GGTGA | 5 cut(s) | 487, 521, 538, 783, 897 |
| AvaII | GGWCC | 2 cut(s) | 25, 1087 |
| AxyI | CCTNAGG | 1 cut(s) | 405 |
| BaeGI | GKGCMC | 2 cut(s) | 350, 463 |
| BalI | TGGCCA | 1 cut(s) | 819 |
| BanII | GRGCYC | 1 cut(s) | 774 |
| BauI | CACGAG | 1 cut(s) | 809 |
| BbsI | GAAGAC | 2 cut(s) | 409, 938 |
| Bbv12I | GWGCWC | 2 cut(s) | 463, 1241 |
| BbvI | GCAGC | 2 cut(s) | 1000, 1044 |
| BccI | CCATC | 3 cut(s) | 308, 479, 506 |
| BciT130I | CCWGG | 4 cut(s) | 497, 775, 1047, 1052 |
| BclI | TGATCA | 1 cut(s) | 142 |
| BcoDI | GTCTC | 1 cut(s) | 953 |
| BfaI | CTAG | 5 cut(s) | 134, 303, 336, 566, 1209 |
| BfmI | CTRYAG | 2 cut(s) | 704, 1014 |
| BfoI | RGCGCY | 1 cut(s) | 1196 |
| BfuAI | ACCTGC | 1 cut(s) | 1141 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1047 |
| BisI | GCNGC | 3 cut(s) | 1014, 1033, 1062 |
| BlsI | GCNGC | 3 cut(s) | 1015, 1034, 1063 |
| Bme1390I | CCNGG | 4 cut(s) | 497, 775, 1047, 1052 |
| Bme18I | GGWCC | 2 cut(s) | 25, 1087 |
| BmgBI | CACGTC | 1 cut(s) | 715 |
| BmgT120I | GGNCC | 4 cut(s) | 25, 1021, 1087, 1141 |
| BmiI | GGNNCC | 2 cut(s) | 26, 391 |
| BmrFI | CCNGG | 4 cut(s) | 497, 775, 1047, 1052 |
| BmrI | ACTGGG | 1 cut(s) | 856 |
| BmsI | GCATC | 4 cut(s) | 45, 837, 846, 927 |
| BmuI | ACTGGG | 1 cut(s) | 856 |
| BpiI | GAAGAC | 2 cut(s) | 409, 938 |
| BpmI | CTGGAG | 1 cut(s) | 1217 |
| BpuEI | CTTGAG | 3 cut(s) | 125, 329, 788 |
| BsaAI | YACGTR | 1 cut(s) | 351 |
| BsaBI | GATNNNNATC | 1 cut(s) | 860 |
| BsaI | GGTCTC | 1 cut(s) | 953 |
| BsaJI | CCNNGG | 5 cut(s) | 50, 773, 820, 1006, 1137 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 510, 540 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 98, 846, 914, 1096, 1150, 1262 |
| Bse1I | ACTGG | 2 cut(s) | 851, 1267 |
| Bse21I | CCTNAGG | 1 cut(s) | 405 |
| Bse8I | GATNNNNATC | 1 cut(s) | 860 |
| BseBI | CCWGG | 4 cut(s) | 497, 775, 1047, 1052 |
| BseDI | CCNNGG | 5 cut(s) | 50, 773, 820, 1006, 1137 |
| BseGI | GGATG | 4 cut(s) | 60, 319, 754, 1002 |
| BseJI | GATNNNNATC | 1 cut(s) | 860 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 98, 846, 914, 1096, 1150, 1262 |
| BseNI | ACTGG | 2 cut(s) | 851, 1267 |
| BseRI | GAGGAG | 1 cut(s) | 531 |
| BseSI | GKGCMC | 2 cut(s) | 350, 463 |
| BseXI | GCAGC | 2 cut(s) | 1000, 1044 |
| BseYI | CCCAGC | 1 cut(s) | 1213 |
| BsgI | GTGCAG | 1 cut(s) | 1144 |
| Bsh1236I | CGCG | 3 cut(s) | 802, 1030, 1234 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1028 |
| BshFI | GGCC | 5 cut(s) | 819, 1022, 1050, 1136, 1142 |
| BsiEI | CGRYCG | 1 cut(s) | 1028 |
| BsiHKAI | GWGCWC | 2 cut(s) | 463, 1241 |
| BslFI | GGGAC | 2 cut(s) | 38, 852 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 98, 846, 914, 1096, 1150, 1262 |
| BsmAI | GTCTC | 1 cut(s) | 953 |
| BsmFI | GGGAC | 2 cut(s) | 38, 852 |
| BsnI | GGCC | 5 cut(s) | 819, 1022, 1050, 1136, 1142 |
| Bso31I | GGTCTC | 1 cut(s) | 953 |
| Bsp1286I | GDGCHC | 4 cut(s) | 350, 463, 774, 1241 |
| Bsp143I | GATC | 4 cut(s) | 46, 142, 429, 788 |
| Bsp19I | CCATGG | 1 cut(s) | 50 |
| BspACI | CCGC | 8 cut(s) | 92, 611, 936, 945, 1028, 1062, 1085, 1234 |
| BspANI | GGCC | 5 cut(s) | 819, 1022, 1050, 1136, 1142 |
| BspFNI | CGCG | 3 cut(s) | 802, 1030, 1234 |
| BspHI | TCATGA | 1 cut(s) | 145 |
| BspLI | GGNNCC | 2 cut(s) | 26, 391 |
| BspMAI | CTGCAG | 1 cut(s) | 1018 |
| BspMI | ACCTGC | 1 cut(s) | 1141 |
| BspPI | GGATC | 2 cut(s) | 41, 437 |
| BspTNI | GGTCTC | 1 cut(s) | 953 |
| BsrI | ACTGG | 2 cut(s) | 851, 1267 |
| BssECI | CCNNGG | 5 cut(s) | 50, 773, 820, 1006, 1137 |
| BssMI | GATC | 4 cut(s) | 46, 142, 429, 788 |
| BssSI | CACGAG | 1 cut(s) | 809 |
| BssT1I | CCWWGG | 3 cut(s) | 50, 820, 1006 |
| Bst2BI | CACGAG | 1 cut(s) | 809 |
| Bst2UI | CCWGG | 4 cut(s) | 497, 775, 1047, 1052 |
| Bst4CI | ACNGT | 2 cut(s) | 29, 795 |
| Bst6I | CTCTTC | 2 cut(s) | 904, 921 |
| BstBAI | YACGTR | 1 cut(s) | 351 |
| BstC8I | GCNNGC | 4 cut(s) | 367, 585, 780, 1077 |
| BstDEI | CTNAG | 1 cut(s) | 405 |
| BstDSI | CCRYGG | 1 cut(s) | 50 |
| BstEII | GGTNACC | 1 cut(s) | 290 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1094 |
| BstF5I | GGATG | 4 cut(s) | 60, 319, 754, 1002 |
| BstFNI | CGCG | 3 cut(s) | 802, 1030, 1234 |
| BstH2I | RGCGCY | 1 cut(s) | 1196 |
| BstHHI | GCGC | 6 cut(s) | 233, 741, 804, 1032, 1195, 1219 |
| BstKTI | GATC | 4 cut(s) | 49, 145, 432, 791 |
| BstMAI | GTCTC | 1 cut(s) | 953 |
| BstMBI | GATC | 4 cut(s) | 46, 142, 429, 788 |
| BstMCI | CGRYCG | 1 cut(s) | 1028 |
| BstNI | CCWGG | 4 cut(s) | 497, 775, 1047, 1052 |
| BstNSI | RCATGY | 3 cut(s) | 98, 587, 621 |
| BstPI | GGTNACC | 1 cut(s) | 290 |
| BstSCI | CCNGG | 4 cut(s) | 495, 773, 1045, 1050 |
| BstSFI | CTRYAG | 2 cut(s) | 704, 1014 |
| BstSLI | GKGCMC | 2 cut(s) | 350, 463 |
| BstUI | CGCG | 3 cut(s) | 802, 1030, 1234 |
| BstV1I | GCAGC | 2 cut(s) | 1000, 1044 |
| BstV2I | GAAGAC | 2 cut(s) | 409, 938 |
| BstX2I | RGATCY | 1 cut(s) | 429 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 815 |
| BstYI | RGATCY | 1 cut(s) | 429 |
| Bsu36I | CCTNAGG | 1 cut(s) | 405 |
| BsuRI | GGCC | 5 cut(s) | 819, 1022, 1050, 1136, 1142 |
| BtgI | CCRYGG | 1 cut(s) | 50 |
| BtrI | CACGTC | 1 cut(s) | 715 |
| BtsCI | GGATG | 4 cut(s) | 60, 319, 754, 1002 |
| BveI | ACCTGC | 1 cut(s) | 1141 |
| Cac8I | GCNNGC | 4 cut(s) | 367, 585, 780, 1077 |
| CciI | TCATGA | 1 cut(s) | 145 |
| CfoI | GCGC | 6 cut(s) | 233, 741, 804, 1032, 1195, 1219 |
| Cfr13I | GGNCC | 4 cut(s) | 25, 1021, 1087, 1141 |
| CseI | GACGC | 1 cut(s) | 808 |
| Csp6I | GTAC | 1 cut(s) | 410 |
| CviQI | GTAC | 1 cut(s) | 410 |
| DdeI | CTNAG | 1 cut(s) | 405 |
| DpnI | GATC | 4 cut(s) | 48, 144, 431, 790 |
| DpnII | GATC | 4 cut(s) | 46, 142, 429, 788 |
| EaeI | YGGCCR | 1 cut(s) | 817 |
| Eam1104I | CTCTTC | 2 cut(s) | 904, 921 |
| EarI | CTCTTC | 2 cut(s) | 904, 921 |
| Eco130I | CCWWGG | 3 cut(s) | 50, 820, 1006 |
| Eco24I | GRGCYC | 1 cut(s) | 774 |
| Eco31I | GGTCTC | 1 cut(s) | 953 |
| Eco47I | GGWCC | 2 cut(s) | 25, 1087 |
| Eco57I | CTGAAG | 1 cut(s) | 977 |
| Eco81I | CCTNAGG | 1 cut(s) | 405 |
| Eco91I | GGTNACC | 1 cut(s) | 290 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1094 |
| EcoO65I | GGTNACC | 1 cut(s) | 290 |
| EcoRII | CCWGG | 4 cut(s) | 495, 773, 1045, 1050 |
| EcoT14I | CCWWGG | 3 cut(s) | 50, 820, 1006 |
| EcoT22I | ATGCAT | 1 cut(s) | 861 |
| EcoT38I | GRGCYC | 1 cut(s) | 774 |
| ErhI | CCWWGG | 3 cut(s) | 50, 820, 1006 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 199, 231 |
| FaqI | GGGAC | 2 cut(s) | 38, 852 |
| FauNDI | CATATG | 1 cut(s) | 422 |
| FbaI | TGATCA | 1 cut(s) | 142 |
| FblI | GTMKAC | 1 cut(s) | 1249 |
| Fnu4HI | GCNGC | 3 cut(s) | 1014, 1033, 1062 |
| FokI | GGATG | 4 cut(s) | 67, 326, 761, 989 |
| FriOI | GRGCYC | 1 cut(s) | 774 |
| Fsp4HI | GCNGC | 3 cut(s) | 1014, 1033, 1062 |
| FspBI | CTAG | 5 cut(s) | 134, 303, 336, 566, 1209 |
| GlaI | GCGC | 6 cut(s) | 232, 740, 803, 1031, 1194, 1218 |
| GluI | GCNGC | 3 cut(s) | 1014, 1033, 1062 |
| GsaI | CCCAGC | 1 cut(s) | 1217 |
| GsuI | CTGGAG | 1 cut(s) | 1217 |
| HaeII | RGCGCY | 1 cut(s) | 1196 |
| HaeIII | GGCC | 5 cut(s) | 819, 1022, 1050, 1136, 1142 |
| HgaI | GACGC | 1 cut(s) | 808 |
| HhaI | GCGC | 6 cut(s) | 233, 741, 804, 1032, 1195, 1219 |
| Hin6I | GCGC | 6 cut(s) | 231, 739, 802, 1030, 1193, 1217 |
| HinP1I | GCGC | 6 cut(s) | 231, 739, 802, 1030, 1193, 1217 |
| HincII | GTYRAC | 1 cut(s) | 798 |
| HindII | GTYRAC | 1 cut(s) | 798 |
| HphI | GGTGA | 5 cut(s) | 487, 521, 538, 783, 897 |
| Hpy166II | GTNNAC | 5 cut(s) | 461, 478, 798, 1226, 1250 |
| Hpy188I | TCNGA | 3 cut(s) | 833, 923, 957 |
| Hpy188III | TCNNGA | 4 cut(s) | 104, 146, 433, 665 |
| Hpy8I | GTNNAC | 5 cut(s) | 461, 478, 798, 1226, 1250 |
| HpyAV | CCTTC | 2 cut(s) | 430, 1126 |
| HpyCH4III | ACNGT | 2 cut(s) | 29, 795 |
| HpyCH4IV | ACGT | 3 cut(s) | 328, 350, 714 |
| HpyF3I | CTNAG | 1 cut(s) | 405 |
| HpySE526I | ACGT | 3 cut(s) | 328, 350, 714 |
| HspAI | GCGC | 6 cut(s) | 231, 739, 802, 1030, 1193, 1217 |
| Ksp22I | TGATCA | 1 cut(s) | 142 |
| Kzo9I | GATC | 4 cut(s) | 46, 142, 429, 788 |
| LmnI | GCTCC | 3 cut(s) | 389, 842, 1236 |
| Lsp1109I | GCAGC | 2 cut(s) | 1000, 1044 |
| LweI | GCATC | 4 cut(s) | 45, 837, 846, 927 |
| MaeI | CTAG | 5 cut(s) | 134, 303, 336, 566, 1209 |
| MaeII | ACGT | 3 cut(s) | 328, 350, 714 |
| MaeIII | GTNAC | 2 cut(s) | 122, 290 |
| MalI | GATC | 4 cut(s) | 48, 144, 431, 790 |
| MboI | GATC | 4 cut(s) | 46, 142, 429, 788 |
| MboII | GAAGA | 8 cut(s) | 149, 409, 582, 731, 891, 898, 938, 943 |
| MflI | RGATCY | 1 cut(s) | 429 |
| MhlI | GDGCHC | 4 cut(s) | 350, 463, 774, 1241 |
| MlsI | TGGCCA | 1 cut(s) | 819 |
| MluCI | AATT | 5 cut(s) | 70, 271, 396, 732, 1172 |
| MluNI | TGGCCA | 1 cut(s) | 819 |
| MmeI | TCCRAC | 1 cut(s) | 147 |
| Mox20I | TGGCCA | 1 cut(s) | 819 |
| Mph1103I | ATGCAT | 1 cut(s) | 861 |
| MscI | TGGCCA | 1 cut(s) | 819 |
| MseI | TTAA | 5 cut(s) | 69, 113, 218, 972, 1184 |
| Msp20I | TGGCCA | 1 cut(s) | 819 |
| MspR9I | CCNGG | 4 cut(s) | 497, 775, 1047, 1052 |
| MvaI | CCWGG | 4 cut(s) | 497, 775, 1047, 1052 |
| MvnI | CGCG | 3 cut(s) | 802, 1030, 1234 |
| NcoI | CCATGG | 1 cut(s) | 50 |
| NdeI | CATATG | 1 cut(s) | 422 |
| NdeII | GATC | 4 cut(s) | 46, 142, 429, 788 |
| NlaIV | GGNNCC | 2 cut(s) | 26, 391 |
| NmeAIII | GCCGAG | 1 cut(s) | 1162 |
| NmuCI | GTSAC | 1 cut(s) | 122 |
| NsiI | ATGCAT | 1 cut(s) | 861 |
| NspI | RCATGY | 3 cut(s) | 98, 587, 621 |
| PaeI | GCATGC | 1 cut(s) | 587 |
| PagI | TCATGA | 1 cut(s) | 145 |
| PaqCI | CACCTGC | 1 cut(s) | 1141 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 846 |
| PkrI | GCNGC | 3 cut(s) | 1015, 1034, 1063 |
| Ppu21I | YACGTR | 1 cut(s) | 351 |
| Psp6I | CCWGG | 4 cut(s) | 495, 773, 1045, 1050 |
| PspEI | GGTNACC | 1 cut(s) | 290 |
| PspFI | CCCAGC | 1 cut(s) | 1213 |
| PspGI | CCWGG | 4 cut(s) | 495, 773, 1045, 1050 |
| PspN4I | GGNNCC | 2 cut(s) | 26, 391 |
| PspPI | GGNCC | 4 cut(s) | 25, 1021, 1087, 1141 |
| PstI | CTGCAG | 1 cut(s) | 1018 |
| PsuI | RGATCY | 1 cut(s) | 429 |
| RsaI | GTAC | 1 cut(s) | 411 |
| RsaNI | GTAC | 1 cut(s) | 410 |
| SaqAI | TTAA | 5 cut(s) | 69, 113, 218, 972, 1184 |
| SatI | GCNGC | 3 cut(s) | 1014, 1033, 1062 |
| Sau3AI | GATC | 4 cut(s) | 46, 142, 429, 788 |
| Sau96I | GGNCC | 4 cut(s) | 25, 1021, 1087, 1141 |
| ScrFI | CCNGG | 4 cut(s) | 497, 775, 1047, 1052 |
| SduI | GDGCHC | 4 cut(s) | 350, 463, 774, 1241 |
| SfaNI | GCATC | 4 cut(s) | 45, 837, 846, 927 |
| SfcI | CTRYAG | 2 cut(s) | 704, 1014 |
| SinI | GGWCC | 2 cut(s) | 25, 1087 |
| SmlI | CTYRAG | 3 cut(s) | 104, 308, 767 |
| SmoI | CTYRAG | 3 cut(s) | 104, 308, 767 |
| SphI | GCATGC | 1 cut(s) | 587 |
| Sse9I | AATT | 5 cut(s) | 70, 271, 396, 732, 1172 |
| SsiI | CCGC | 8 cut(s) | 92, 611, 936, 945, 1028, 1062, 1085, 1234 |
| SspMI | CTAG | 5 cut(s) | 134, 303, 336, 566, 1209 |
| StyD4I | CCNGG | 4 cut(s) | 495, 773, 1045, 1050 |
| StyI | CCWWGG | 3 cut(s) | 50, 820, 1006 |
| TaaI | ACNGT | 2 cut(s) | 29, 795 |
| TaiI | ACGT | 3 cut(s) | 331, 353, 717 |
| TaqI | TCGA | 2 cut(s) | 189, 875 |
| TasI | AATT | 5 cut(s) | 70, 271, 396, 732, 1172 |
| TauI | GCSGC | 1 cut(s) | 1064 |
| Tru1I | TTAA | 5 cut(s) | 69, 113, 218, 972, 1184 |
| Tru9I | TTAA | 5 cut(s) | 69, 113, 218, 972, 1184 |
| TseFI | GTSAC | 1 cut(s) | 122 |
| TseI | GCWGC | 2 cut(s) | 1013, 1032 |
| Tsp45I | GTSAC | 1 cut(s) | 122 |
| TspDTI | ATGAA | 4 cut(s) | 350, 409, 555, 744 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 846 |
| VneI | GTGCAC | 1 cut(s) | 459 |
| VpaK11BI | GGWCC | 2 cut(s) | 25, 1087 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1094 |
| XapI | RAATTY | 2 cut(s) | 396, 1172 |
| XceI | RCATGY | 3 cut(s) | 98, 587, 621 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 503 |
| XmiI | GTMKAC | 1 cut(s) | 1249 |
| XspI | CTAG | 5 cut(s) | 134, 303, 336, 566, 1209 |
| Zsp2I | ATGCAT | 1 cut(s) | 861 |