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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 214 |
| AccB1I | GGYRCC | 1 cut(s) | 214 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AccII | CGCG | 1 cut(s) | 555 |
| AciI | CCGC | 5 cut(s) | 20, 298, 333, 447, 510 |
| AclWI | GGATC | 1 cut(s) | 461 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AcuI | CTGAAG | 1 cut(s) | 140 |
| AfaI | GTAC | 3 cut(s) | 175, 216, 612 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 283, 310, 343, 435 |
| AgsI | TTSAA | 3 cut(s) | 542, 596, 625 |
| AjnI | CCWGG | 5 cut(s) | 176, 278, 428, 433, 449 |
| AluBI | AGCT | 3 cut(s) | 236, 289, 630 |
| AluI | AGCT | 3 cut(s) | 236, 289, 630 |
| Alw21I | GWGCWC | 1 cut(s) | 48 |
| AlwI | GGATC | 1 cut(s) | 461 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 289 |
| AoxI | GGCC | 5 cut(s) | 75, 90, 371, 431, 437 |
| ApeKI | GCWGC | 3 cut(s) | 236, 295, 355 |
| Asp718I | GGTACC | 1 cut(s) | 214 |
| AspLEI | GCGC | 3 cut(s) | 130, 154, 557 |
| AspS9I | GGNCC | 4 cut(s) | 22, 76, 315, 437 |
| AsuHPI | GGTGA | 1 cut(s) | 436 |
| AvaII | GGWCC | 2 cut(s) | 22, 315 |
| BaeGI | GKGCMC | 1 cut(s) | 568 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BanI | GGYRCC | 1 cut(s) | 214 |
| Bbv12I | GWGCWC | 1 cut(s) | 48 |
| BbvI | GCAGC | 3 cut(s) | 223, 307, 367 |
| BccI | CCATC | 3 cut(s) | 236, 254, 314 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 308, 342 |
| BciT130I | CCWGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| BfaI | CTAG | 2 cut(s) | 149, 252 |
| BfmI | CTRYAG | 1 cut(s) | 209 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| BisI | GCNGC | 4 cut(s) | 237, 296, 356, 511 |
| BlsI | GCNGC | 4 cut(s) | 238, 297, 357, 512 |
| Bme1390I | CCNGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| Bme18I | GGWCC | 2 cut(s) | 22, 315 |
| BmgT120I | GGNCC | 4 cut(s) | 22, 76, 315, 437 |
| BmiI | GGNNCC | 3 cut(s) | 216, 302, 316 |
| BmrFI | CCNGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| BmrI | ACTGGG | 1 cut(s) | 320 |
| BmsI | GCATC | 2 cut(s) | 316, 342 |
| BmuI | ACTGGG | 1 cut(s) | 320 |
| BpmI | CTGGAG | 1 cut(s) | 152 |
| BsaBI | GATNNNNATC | 1 cut(s) | 264 |
| BsaJI | CCNNGG | 3 cut(s) | 278, 434, 614 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 283, 310, 343, 435 |
| Bse1I | ACTGG | 1 cut(s) | 315 |
| Bse8I | GATNNNNATC | 1 cut(s) | 264 |
| BseBI | CCWGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| BseDI | CCNNGG | 3 cut(s) | 278, 434, 614 |
| BseGI | GGATG | 2 cut(s) | 357, 425 |
| BseJI | GATNNNNATC | 1 cut(s) | 264 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 283, 310, 343, 435 |
| BseNI | ACTGG | 1 cut(s) | 315 |
| BseSI | GKGCMC | 1 cut(s) | 568 |
| BseXI | GCAGC | 3 cut(s) | 223, 307, 367 |
| BsgI | GTGCAG | 1 cut(s) | 314 |
| Bsh1236I | CGCG | 1 cut(s) | 555 |
| BshFI | GGCC | 5 cut(s) | 77, 92, 373, 433, 439 |
| BshNI | GGYRCC | 1 cut(s) | 214 |
| BsiHKAI | GWGCWC | 1 cut(s) | 48 |
| BslFI | GGGAC | 1 cut(s) | 260 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 283, 310, 343, 435 |
| BsmFI | GGGAC | 1 cut(s) | 260 |
| BsmI | GAATGC | 1 cut(s) | 548 |
| BsnI | GGCC | 5 cut(s) | 77, 92, 373, 433, 439 |
| Bsp1286I | GDGCHC | 2 cut(s) | 48, 568 |
| Bsp143I | GATC | 1 cut(s) | 453 |
| BspACI | CCGC | 5 cut(s) | 20, 298, 333, 447, 510 |
| BspANI | GGCC | 5 cut(s) | 77, 92, 373, 433, 439 |
| BspFNI | CGCG | 1 cut(s) | 555 |
| BspLI | GGNNCC | 3 cut(s) | 216, 302, 316 |
| BspPI | GGATC | 1 cut(s) | 461 |
| BspT107I | GGYRCC | 1 cut(s) | 214 |
| BsrI | ACTGG | 1 cut(s) | 315 |
| BssECI | CCNNGG | 3 cut(s) | 278, 434, 614 |
| BssMI | GATC | 1 cut(s) | 453 |
| Bst2UI | CCWGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| Bst4CI | ACNGT | 1 cut(s) | 570 |
| BstC8I | GCNNGC | 3 cut(s) | 161, 199, 391 |
| BstDSI | CCRYGG | 1 cut(s) | 614 |
| BstF5I | GGATG | 2 cut(s) | 357, 425 |
| BstFNI | CGCG | 1 cut(s) | 555 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 3 cut(s) | 130, 154, 557 |
| BstKTI | GATC | 1 cut(s) | 456 |
| BstMBI | GATC | 1 cut(s) | 453 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 134, 149, 160, 295, 364, 496, 552, 563 |
| BstNI | CCWGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| BstSCI | CCNGG | 5 cut(s) | 176, 278, 428, 433, 449 |
| BstSFI | CTRYAG | 1 cut(s) | 209 |
| BstSLI | GKGCMC | 1 cut(s) | 568 |
| BstUI | CGCG | 1 cut(s) | 555 |
| BstV1I | GCAGC | 3 cut(s) | 223, 307, 367 |
| BstX2I | RGATCY | 1 cut(s) | 453 |
| BstYI | RGATCY | 1 cut(s) | 453 |
| BsuRI | GGCC | 5 cut(s) | 77, 92, 373, 433, 439 |
| BtgI | CCRYGG | 1 cut(s) | 614 |
| BtgZI | GCGATG | 1 cut(s) | 339 |
| BtsCI | GGATG | 2 cut(s) | 357, 425 |
| Cac8I | GCNNGC | 3 cut(s) | 161, 199, 391 |
| CaiI | CAGNNNCTG | 1 cut(s) | 289 |
| CfoI | GCGC | 3 cut(s) | 130, 154, 557 |
| Cfr13I | GGNCC | 4 cut(s) | 22, 76, 315, 437 |
| CseI | GACGC | 1 cut(s) | 496 |
| Csp6I | GTAC | 3 cut(s) | 174, 215, 611 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 603 |
| CviAII | CATG | 1 cut(s) | 514 |
| CviQI | GTAC | 3 cut(s) | 174, 215, 611 |
| DpnI | GATC | 1 cut(s) | 455 |
| DpnII | GATC | 1 cut(s) | 453 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| EciI | GGCGGA | 1 cut(s) | 35 |
| Eco47I | GGWCC | 2 cut(s) | 22, 315 |
| Eco57I | CTGAAG | 1 cut(s) | 140 |
| EcoRII | CCWGG | 5 cut(s) | 176, 278, 428, 433, 449 |
| FaeI | CATG | 1 cut(s) | 517 |
| FaiI | YATR | 6 cut(s) | 6, 30, 224, 478, 480, 515 |
| FaqI | GGGAC | 1 cut(s) | 260 |
| FatI | CATG | 1 cut(s) | 513 |
| FauNDI | CATATG | 1 cut(s) | 478 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 4 cut(s) | 237, 296, 356, 511 |
| FokI | GGATG | 2 cut(s) | 364, 412 |
| Fsp4HI | GCNGC | 4 cut(s) | 237, 296, 356, 511 |
| FspBI | CTAG | 2 cut(s) | 149, 252 |
| GlaI | GCGC | 3 cut(s) | 129, 153, 556 |
| GluI | GCNGC | 4 cut(s) | 237, 296, 356, 511 |
| GsuI | CTGGAG | 1 cut(s) | 152 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 5 cut(s) | 77, 92, 373, 433, 439 |
| HgaI | GACGC | 1 cut(s) | 496 |
| HhaI | GCGC | 3 cut(s) | 130, 154, 557 |
| Hin1II | CATG | 1 cut(s) | 517 |
| Hin6I | GCGC | 3 cut(s) | 128, 152, 555 |
| HinP1I | GCGC | 3 cut(s) | 128, 152, 555 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 1 cut(s) | 108 |
| HphI | GGTGA | 1 cut(s) | 436 |
| Hpy166II | GTNNAC | 1 cut(s) | 188 |
| Hpy188I | TCNGA | 3 cut(s) | 233, 319, 470 |
| Hpy188III | TCNNGA | 2 cut(s) | 105, 379 |
| Hpy8I | GTNNAC | 1 cut(s) | 188 |
| Hpy99I | CGWCG | 1 cut(s) | 116 |
| HpyAV | CCTTC | 1 cut(s) | 118 |
| HpyCH4III | ACNGT | 1 cut(s) | 570 |
| HpyCH4V | TGCA | 5 cut(s) | 295, 329, 355, 482, 546 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 134, 149, 160, 295, 364, 496, 552, 563 |
| Hsp92II | CATG | 1 cut(s) | 517 |
| HspAI | GCGC | 3 cut(s) | 128, 152, 555 |
| KpnI | GGTACC | 1 cut(s) | 218 |
| Kzo9I | GATC | 1 cut(s) | 453 |
| LmnI | GCTCC | 2 cut(s) | 43, 286 |
| Lsp1109I | GCAGC | 3 cut(s) | 223, 307, 367 |
| LweI | GCATC | 2 cut(s) | 316, 342 |
| MaeI | CTAG | 2 cut(s) | 149, 252 |
| MalI | GATC | 1 cut(s) | 455 |
| MboI | GATC | 1 cut(s) | 453 |
| MboII | GAAGA | 1 cut(s) | 133 |
| MflI | RGATCY | 1 cut(s) | 453 |
| MhlI | GDGCHC | 2 cut(s) | 48, 568 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluCI | AATT | 2 cut(s) | 359, 603 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MmeI | TCCRAC | 2 cut(s) | 331, 493 |
| MnlI | CCTC | 5 cut(s) | 35, 67, 127, 314, 379 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspA1I | CMGCKG | 2 cut(s) | 298, 510 |
| MspR9I | CCNGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| Mva1269I | GAATGC | 1 cut(s) | 548 |
| MvaI | CCWGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| MvnI | CGCG | 1 cut(s) | 555 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 134, 149, 160, 295, 364, 496, 552, 563 |
| NdeI | CATATG | 1 cut(s) | 478 |
| NdeII | GATC | 1 cut(s) | 453 |
| NlaIII | CATG | 1 cut(s) | 517 |
| NlaIV | GGNNCC | 3 cut(s) | 216, 302, 316 |
| PctI | GAATGC | 1 cut(s) | 548 |
| PfeI | GAWTC | 1 cut(s) | 108 |
| PkrI | GCNGC | 4 cut(s) | 238, 297, 357, 512 |
| Psp6I | CCWGG | 5 cut(s) | 176, 278, 428, 433, 449 |
| PspGI | CCWGG | 5 cut(s) | 176, 278, 428, 433, 449 |
| PspN4I | GGNNCC | 3 cut(s) | 216, 302, 316 |
| PspPI | GGNCC | 4 cut(s) | 22, 76, 315, 437 |
| PstNI | CAGNNNCTG | 1 cut(s) | 289 |
| PsuI | RGATCY | 1 cut(s) | 453 |
| RsaI | GTAC | 3 cut(s) | 175, 216, 612 |
| RsaNI | GTAC | 3 cut(s) | 174, 215, 611 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SatI | GCNGC | 4 cut(s) | 237, 296, 356, 511 |
| Sau3AI | GATC | 1 cut(s) | 453 |
| Sau96I | GGNCC | 4 cut(s) | 22, 76, 315, 437 |
| ScrFI | CCNGG | 5 cut(s) | 178, 280, 430, 435, 451 |
| SduI | GDGCHC | 2 cut(s) | 48, 568 |
| SfaNI | GCATC | 2 cut(s) | 316, 342 |
| SfcI | CTRYAG | 1 cut(s) | 209 |
| SinI | GGWCC | 2 cut(s) | 22, 315 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| Sse9I | AATT | 2 cut(s) | 359, 603 |
| SsiI | CCGC | 5 cut(s) | 20, 298, 333, 447, 510 |
| SspI | AATATT | 1 cut(s) | 591 |
| SspMI | CTAG | 2 cut(s) | 149, 252 |
| StyD4I | CCNGG | 5 cut(s) | 176, 278, 428, 433, 449 |
| TaaI | ACNGT | 1 cut(s) | 570 |
| TaqI | TCGA | 2 cut(s) | 111, 187 |
| TasI | AATT | 2 cut(s) | 359, 603 |
| TauI | GCSGC | 1 cut(s) | 513 |
| TfiI | GAWTC | 1 cut(s) | 108 |
| TseI | GCWGC | 3 cut(s) | 236, 295, 355 |
| VpaK11BI | GGWCC | 2 cut(s) | 22, 315 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 286 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 2 cut(s) | 149, 252 |