21 kDa protein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 796 |
| Acc36I | ACCTGC | 1 cut(s) | 796 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 396 |
| AccBSI | CCGCTC | 1 cut(s) | 687 |
| AccII | CGCG | 2 cut(s) | 352, 889 |
| AciI | CCGC | 9 cut(s) | 314, 504, 578, 624, 671, 687, 717, 740, 889 |
| AclI | AACGTT | 2 cut(s) | 269, 585 |
| AclWI | GGATC | 1 cut(s) | 684 |
| AcoI | YGGCCR | 1 cut(s) | 810 |
| AcsI | RAATTY | 2 cut(s) | 236, 249 |
| AfaI | GTAC | 2 cut(s) | 70, 204 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 396, 625, 805, 917 |
| AflII | CTTAAG | 1 cut(s) | 839 |
| AflIII | ACRYGT | 1 cut(s) | 350 |
| AgeI | ACCGGT | 1 cut(s) | 917 |
| AgsI | TTSAA | 4 cut(s) | 236, 276, 284, 427 |
| AjnI | CCWGG | 2 cut(s) | 867, 896 |
| AluBI | AGCT | 9 cut(s) | 8, 148, 209, 310, 387, 396, 446, 730, 838 |
| AluI | AGCT | 9 cut(s) | 8, 148, 209, 310, 387, 396, 446, 730, 838 |
| Alw21I | GWGCWC | 1 cut(s) | 896 |
| AlwI | GGATC | 1 cut(s) | 684 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 396 |
| AoxI | GGCC | 5 cut(s) | 469, 742, 789, 795, 810 |
| ApeKI | GCWGC | 1 cut(s) | 23 |
| ApoI | RAATTY | 2 cut(s) | 236, 249 |
| AsiGI | ACCGGT | 1 cut(s) | 917 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 85 |
| AspLEI | GCGC | 3 cut(s) | 850, 874, 903 |
| AspS9I | GGNCC | 6 cut(s) | 293, 649, 709, 742, 796, 935 |
| AsuC2I | CCSGG | 2 cut(s) | 325, 933 |
| AsuHPI | GGTGA | 3 cut(s) | 333, 447, 706 |
| AvaII | GGWCC | 4 cut(s) | 293, 649, 709, 935 |
| BalI | TGGCCA | 1 cut(s) | 812 |
| BanII | GRGCYC | 1 cut(s) | 324 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 52, 84 |
| Bbv12I | GWGCWC | 1 cut(s) | 896 |
| BbvI | GCAGC | 1 cut(s) | 10 |
| BccI | CCATC | 3 cut(s) | 480, 494, 552 |
| BciT130I | CCWGG | 2 cut(s) | 869, 898 |
| BclI | TGATCA | 2 cut(s) | 526, 561 |
| BcnI | CCSGG | 2 cut(s) | 325, 933 |
| BfaI | CTAG | 2 cut(s) | 206, 737 |
| BfmI | CTRYAG | 2 cut(s) | 24, 666 |
| BfoI | RGCGCY | 1 cut(s) | 851 |
| BfrI | CTTAAG | 1 cut(s) | 839 |
| BfuAI | ACCTGC | 1 cut(s) | 796 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 869 |
| BisI | GCNGC | 2 cut(s) | 24, 688 |
| BlsI | GCNGC | 2 cut(s) | 25, 689 |
| BmcAI | AGTACT | 1 cut(s) | 204 |
| Bme1390I | CCNGG | 4 cut(s) | 325, 869, 898, 933 |
| Bme18I | GGWCC | 4 cut(s) | 293, 649, 709, 935 |
| BmgT120I | GGNCC | 6 cut(s) | 293, 649, 709, 742, 796, 935 |
| BmiI | GGNNCC | 3 cut(s) | 294, 630, 651 |
| BmrFI | CCNGG | 4 cut(s) | 325, 869, 898, 933 |
| BmrI | ACTGGG | 1 cut(s) | 551 |
| BmuI | ACTGGG | 1 cut(s) | 551 |
| BpmI | CTGGAG | 1 cut(s) | 373 |
| Bpu10I | CCTNAGC | 2 cut(s) | 144, 318 |
| BpuMI | CCSGG | 2 cut(s) | 325, 933 |
| BsaJI | CCNNGG | 2 cut(s) | 323, 792 |
| BsaWI | WCCGGW | 2 cut(s) | 77, 917 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 396, 625, 805, 917 |
| Bse118I | RCCGGY | 1 cut(s) | 917 |
| Bse1I | ACTGG | 2 cut(s) | 557, 639 |
| Bse3DI | GCAATG | 1 cut(s) | 133 |
| BseBI | CCWGG | 2 cut(s) | 869, 898 |
| BseDI | CCNNGG | 2 cut(s) | 323, 792 |
| BseGI | GGATG | 3 cut(s) | 472, 482, 544 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 396, 625, 805, 917 |
| BseMI | GCAATG | 1 cut(s) | 133 |
| BseMII | CTCAG | 4 cut(s) | 235, 309, 507, 659 |
| BseNI | ACTGG | 2 cut(s) | 557, 639 |
| BseRI | GAGGAG | 1 cut(s) | 442 |
| BseXI | GCAGC | 1 cut(s) | 10 |
| BseYI | CCCAGC | 2 cut(s) | 396, 620 |
| BsgI | GTGCAG | 1 cut(s) | 725 |
| Bsh1236I | CGCG | 2 cut(s) | 352, 889 |
| BshFI | GGCC | 5 cut(s) | 471, 744, 791, 797, 812 |
| BshTI | ACCGGT | 1 cut(s) | 917 |
| BsiHKAI | GWGCWC | 1 cut(s) | 896 |
| BsiSI | CCGG | 4 cut(s) | 78, 325, 918, 933 |
| BslFI | GGGAC | 3 cut(s) | 402, 414, 635 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 396, 625, 805, 917 |
| BsmFI | GGGAC | 3 cut(s) | 402, 414, 635 |
| BsmI | GAATGC | 1 cut(s) | 770 |
| BsnI | GGCC | 5 cut(s) | 471, 744, 791, 797, 812 |
| Bsp1286I | GDGCHC | 2 cut(s) | 324, 896 |
| Bsp143I | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| BspACI | CCGC | 9 cut(s) | 314, 504, 578, 624, 671, 687, 717, 740, 889 |
| BspANI | GGCC | 5 cut(s) | 471, 744, 791, 797, 812 |
| BspCNI | CTCAG | 4 cut(s) | 234, 310, 508, 658 |
| BspFNI | CGCG | 2 cut(s) | 352, 889 |
| BspHI | TCATGA | 1 cut(s) | 489 |
| BspLI | GGNNCC | 3 cut(s) | 294, 630, 651 |
| BspMAI | CTGCAG | 1 cut(s) | 28 |
| BspMI | ACCTGC | 1 cut(s) | 796 |
| BspPI | GGATC | 1 cut(s) | 684 |
| BspTI | CTTAAG | 1 cut(s) | 839 |
| BsrBI | CCGCTC | 1 cut(s) | 687 |
| BsrDI | GCAATG | 1 cut(s) | 133 |
| BsrFI | RCCGGY | 1 cut(s) | 917 |
| BsrI | ACTGG | 2 cut(s) | 557, 639 |
| BssAI | RCCGGY | 1 cut(s) | 917 |
| BssECI | CCNNGG | 2 cut(s) | 323, 792 |
| BssMI | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| Bst2UI | CCWGG | 2 cut(s) | 869, 898 |
| Bst4CI | ACNGT | 1 cut(s) | 500 |
| BstAFI | CTTAAG | 1 cut(s) | 839 |
| BstC8I | GCNNGC | 7 cut(s) | 21, 308, 522, 732, 742, 770, 881 |
| BstDEI | CTNAG | 5 cut(s) | 144, 221, 318, 516, 645 |
| BstF5I | GGATG | 3 cut(s) | 472, 482, 544 |
| BstFNI | CGCG | 2 cut(s) | 352, 889 |
| BstH2I | RGCGCY | 1 cut(s) | 851 |
| BstHHI | GCGC | 3 cut(s) | 850, 874, 903 |
| BstKTI | GATC | 5 cut(s) | 341, 443, 529, 564, 679 |
| BstMBI | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| BstNI | CCWGG | 2 cut(s) | 869, 898 |
| BstNSI | RCATGY | 1 cut(s) | 524 |
| BstSCI | CCNGG | 4 cut(s) | 323, 867, 896, 931 |
| BstSFI | CTRYAG | 2 cut(s) | 24, 666 |
| BstUI | CGCG | 2 cut(s) | 352, 889 |
| BstV1I | GCAGC | 1 cut(s) | 10 |
| BsuRI | GGCC | 5 cut(s) | 471, 744, 791, 797, 812 |
| BtsCI | GGATG | 3 cut(s) | 472, 482, 544 |
| BtsIMutI | CAGTG | 2 cut(s) | 513, 564 |
| BveI | ACCTGC | 1 cut(s) | 796 |
| Cac8I | GCNNGC | 7 cut(s) | 21, 308, 522, 732, 742, 770, 881 |
| CaiI | CAGNNNCTG | 1 cut(s) | 396 |
| CciI | TCATGA | 1 cut(s) | 489 |
| CfoI | GCGC | 3 cut(s) | 850, 874, 903 |
| Cfr10I | RCCGGY | 1 cut(s) | 917 |
| Cfr13I | GGNCC | 6 cut(s) | 293, 649, 709, 742, 796, 935 |
| CseI | GACGC | 3 cut(s) | 214, 341, 358 |
| Csp6I | GTAC | 2 cut(s) | 69, 203 |
| CspAI | ACCGGT | 1 cut(s) | 917 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 901, 936 |
| CviAII | CATG | 5 cut(s) | 123, 254, 432, 490, 521 |
| CviQI | GTAC | 2 cut(s) | 69, 203 |
| DdeI | CTNAG | 5 cut(s) | 144, 221, 318, 516, 645 |
| DpnI | GATC | 5 cut(s) | 340, 442, 528, 563, 678 |
| DpnII | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| EaeI | YGGCCR | 1 cut(s) | 810 |
| Eco24I | GRGCYC | 1 cut(s) | 324 |
| Eco47I | GGWCC | 4 cut(s) | 293, 649, 709, 935 |
| EcoO109I | RGGNCCY | 3 cut(s) | 293, 649, 709 |
| EcoRII | CCWGG | 2 cut(s) | 867, 896 |
| EcoT38I | GRGCYC | 1 cut(s) | 324 |
| FaeI | CATG | 5 cut(s) | 126, 257, 435, 493, 524 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 29 |
| FaqI | GGGAC | 3 cut(s) | 402, 414, 635 |
| FatI | CATG | 5 cut(s) | 122, 253, 431, 489, 520 |
| FauI | CCCGC | 3 cut(s) | 497, 617, 733 |
| FbaI | TGATCA | 2 cut(s) | 526, 561 |
| Fnu4HI | GCNGC | 2 cut(s) | 24, 688 |
| FokI | GGATG | 3 cut(s) | 459, 469, 531 |
| FriOI | GRGCYC | 1 cut(s) | 324 |
| Fsp4HI | GCNGC | 2 cut(s) | 24, 688 |
| FspBI | CTAG | 2 cut(s) | 206, 737 |
| GlaI | GCGC | 3 cut(s) | 849, 873, 902 |
| GluI | GCNGC | 2 cut(s) | 24, 688 |
| GsaI | CCCAGC | 2 cut(s) | 400, 624 |
| GsuI | CTGGAG | 1 cut(s) | 373 |
| HaeII | RGCGCY | 1 cut(s) | 851 |
| HaeIII | GGCC | 5 cut(s) | 471, 744, 791, 797, 812 |
| HapII | CCGG | 4 cut(s) | 78, 325, 918, 933 |
| HgaI | GACGC | 3 cut(s) | 214, 341, 358 |
| HhaI | GCGC | 3 cut(s) | 850, 874, 903 |
| Hin1II | CATG | 5 cut(s) | 126, 257, 435, 493, 524 |
| Hin6I | GCGC | 3 cut(s) | 848, 872, 901 |
| HinP1I | GCGC | 3 cut(s) | 848, 872, 901 |
| HincII | GTYRAC | 3 cut(s) | 583, 589, 616 |
| HindII | GTYRAC | 3 cut(s) | 583, 589, 616 |
| HindIII | AAGCTT | 1 cut(s) | 6 |
| HpaI | GTTAAC | 2 cut(s) | 583, 589 |
| HpaII | CCGG | 4 cut(s) | 78, 325, 918, 933 |
| HphI | GGTGA | 3 cut(s) | 333, 447, 706 |
| Hpy166II | GTNNAC | 3 cut(s) | 583, 589, 616 |
| Hpy188I | TCNGA | 1 cut(s) | 348 |
| Hpy188III | TCNNGA | 4 cut(s) | 233, 390, 490, 825 |
| Hpy8I | GTNNAC | 3 cut(s) | 583, 589, 616 |
| Hpy99I | CGWCG | 2 cut(s) | 230, 357 |
| HpyAV | CCTTC | 2 cut(s) | 461, 755 |
| HpyCH4III | ACNGT | 1 cut(s) | 500 |
| HpyCH4IV | ACGT | 4 cut(s) | 269, 567, 585, 608 |
| HpyCH4V | TGCA | 5 cut(s) | 26, 43, 126, 596, 706 |
| HpyF3I | CTNAG | 5 cut(s) | 144, 221, 318, 516, 645 |
| HpySE526I | ACGT | 4 cut(s) | 269, 567, 585, 608 |
| Hsp92II | CATG | 5 cut(s) | 126, 257, 435, 493, 524 |
| HspAI | GCGC | 3 cut(s) | 848, 872, 901 |
| Ksp22I | TGATCA | 2 cut(s) | 526, 561 |
| KspAI | GTTAAC | 2 cut(s) | 583, 589 |
| Kzo9I | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| LmnI | GCTCC | 3 cut(s) | 392, 775, 891 |
| Lsp1109I | GCAGC | 1 cut(s) | 10 |
| MaeI | CTAG | 2 cut(s) | 206, 737 |
| MaeII | ACGT | 4 cut(s) | 269, 567, 585, 608 |
| MaeIII | GTNAC | 2 cut(s) | 256, 905 |
| MalI | GATC | 5 cut(s) | 340, 442, 528, 563, 678 |
| MbiI | CCGCTC | 1 cut(s) | 687 |
| MboI | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| MboII | GAAGA | 1 cut(s) | 450 |
| MfeI | CAATTG | 1 cut(s) | 44 |
| MhlI | GDGCHC | 2 cut(s) | 324, 896 |
| MlsI | TGGCCA | 1 cut(s) | 812 |
| MluCI | AATT | 3 cut(s) | 44, 236, 249 |
| MluI | ACGCGT | 1 cut(s) | 350 |
| MluNI | TGGCCA | 1 cut(s) | 812 |
| MnlI | CCTC | 9 cut(s) | 306, 354, 375, 463, 667, 708, 722, 787, 837 |
| Mox20I | TGGCCA | 1 cut(s) | 812 |
| MroXI | GAANNNNTTC | 1 cut(s) | 85 |
| MscI | TGGCCA | 1 cut(s) | 812 |
| MseI | TTAA | 4 cut(s) | 51, 582, 588, 840 |
| MslI | CAYNNNNRTG | 2 cut(s) | 719, 818 |
| Msp20I | TGGCCA | 1 cut(s) | 812 |
| MspA1I | CMGCKG | 3 cut(s) | 578, 624, 671 |
| MspCI | CTTAAG | 1 cut(s) | 839 |
| MspI | CCGG | 4 cut(s) | 78, 325, 918, 933 |
| MspR9I | CCNGG | 4 cut(s) | 325, 869, 898, 933 |
| MunI | CAATTG | 1 cut(s) | 44 |
| Mva1269I | GAATGC | 1 cut(s) | 770 |
| MvaI | CCWGG | 2 cut(s) | 869, 898 |
| MvnI | CGCG | 2 cut(s) | 352, 889 |
| NciI | CCSGG | 2 cut(s) | 325, 933 |
| NdeII | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| NlaIII | CATG | 5 cut(s) | 126, 257, 435, 493, 524 |
| NlaIV | GGNNCC | 3 cut(s) | 294, 630, 651 |
| NmeAIII | GCCGAG | 2 cut(s) | 708, 817 |
| NmuCI | GTSAC | 1 cut(s) | 256 |
| NspI | RCATGY | 1 cut(s) | 524 |
| PaeI | GCATGC | 1 cut(s) | 524 |
| PagI | TCATGA | 1 cut(s) | 489 |
| PaqCI | CACCTGC | 1 cut(s) | 796 |
| PctI | GAATGC | 1 cut(s) | 770 |
| PdmI | GAANNNNTTC | 1 cut(s) | 85 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 396 |
| PinAI | ACCGGT | 1 cut(s) | 917 |
| PkrI | GCNGC | 2 cut(s) | 25, 689 |
| PpuMI | RGGWCCY | 3 cut(s) | 293, 649, 709 |
| Psp1406I | AACGTT | 2 cut(s) | 269, 585 |
| Psp5II | RGGWCCY | 3 cut(s) | 293, 649, 709 |
| Psp6I | CCWGG | 2 cut(s) | 867, 896 |
| PspFI | CCCAGC | 2 cut(s) | 396, 620 |
| PspGI | CCWGG | 2 cut(s) | 867, 896 |
| PspN4I | GGNNCC | 3 cut(s) | 294, 630, 651 |
| PspPI | GGNCC | 6 cut(s) | 293, 649, 709, 742, 796, 935 |
| PspPPI | RGGWCCY | 3 cut(s) | 293, 649, 709 |
| PstI | CTGCAG | 1 cut(s) | 28 |
| PstNI | CAGNNNCTG | 1 cut(s) | 396 |
| RsaI | GTAC | 2 cut(s) | 70, 204 |
| RsaNI | GTAC | 2 cut(s) | 69, 203 |
| RseI | CAYNNNNRTG | 2 cut(s) | 719, 818 |
| SaqAI | TTAA | 4 cut(s) | 51, 582, 588, 840 |
| SatI | GCNGC | 2 cut(s) | 24, 688 |
| Sau3AI | GATC | 5 cut(s) | 338, 440, 526, 561, 676 |
| Sau96I | GGNCC | 6 cut(s) | 293, 649, 709, 742, 796, 935 |
| ScaI | AGTACT | 1 cut(s) | 204 |
| ScrFI | CCNGG | 4 cut(s) | 325, 869, 898, 933 |
| SduI | GDGCHC | 2 cut(s) | 324, 896 |
| SfcI | CTRYAG | 2 cut(s) | 24, 666 |
| SgrAI | CRCCGGYG | 1 cut(s) | 917 |
| SinI | GGWCC | 4 cut(s) | 293, 649, 709, 935 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 719, 818 |
| SmlI | CTYRAG | 1 cut(s) | 839 |
| SmoI | CTYRAG | 1 cut(s) | 839 |
| SphI | GCATGC | 1 cut(s) | 524 |
| Sse9I | AATT | 3 cut(s) | 44, 236, 249 |
| SsiI | CCGC | 9 cut(s) | 314, 504, 578, 624, 671, 687, 717, 740, 889 |
| SspMI | CTAG | 2 cut(s) | 206, 737 |
| StyD4I | CCNGG | 4 cut(s) | 323, 867, 896, 931 |
| TaaI | ACNGT | 1 cut(s) | 500 |
| TaiI | ACGT | 4 cut(s) | 272, 570, 588, 611 |
| TaqI | TCGA | 1 cut(s) | 824 |
| TasI | AATT | 3 cut(s) | 44, 236, 249 |
| TatI | WGTACW | 2 cut(s) | 68, 202 |
| TauI | GCSGC | 1 cut(s) | 690 |
| Tru1I | TTAA | 4 cut(s) | 51, 582, 588, 840 |
| Tru9I | TTAA | 4 cut(s) | 51, 582, 588, 840 |
| TscAI | CASTG | 2 cut(s) | 520, 564 |
| TseFI | GTSAC | 1 cut(s) | 256 |
| TseI | GCWGC | 1 cut(s) | 23 |
| Tsp45I | GTSAC | 1 cut(s) | 256 |
| TspDTI | ATGAA | 5 cut(s) | 17, 100, 242, 278, 592 |
| TspRI | CASTG | 2 cut(s) | 520, 564 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 396 |
| Vha464I | CTTAAG | 1 cut(s) | 839 |
| VpaK11BI | GGWCC | 4 cut(s) | 293, 649, 709, 935 |
| XapI | RAATTY | 2 cut(s) | 236, 249 |
| XceI | RCATGY | 1 cut(s) | 524 |
| XmnI | GAANNNNTTC | 1 cut(s) | 85 |
| XspI | CTAG | 2 cut(s) | 206, 737 |
| ZrmI | AGTACT | 1 cut(s) | 204 |