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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 377 |
| Acc16I | TGCGCA | 1 cut(s) | 373 |
| Acc36I | ACCTGC | 3 cut(s) | 377, 394, 522 |
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 3 cut(s) | 365, 379, 406 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccII | CGCG | 1 cut(s) | 90 |
| AclWI | GGATC | 2 cut(s) | 168, 201 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 2 cut(s) | 48, 311 |
| AcyI | GRCGYC | 1 cut(s) | 120 |
| AfaI | GTAC | 4 cut(s) | 176, 381, 408, 548 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 77, 151, 508 |
| AjnI | CCWGG | 3 cut(s) | 133, 156, 347 |
| AluBI | AGCT | 2 cut(s) | 74, 460 |
| AluI | AGCT | 2 cut(s) | 74, 460 |
| AlwI | GGATC | 2 cut(s) | 168, 201 |
| Ama87I | CYCGRG | 1 cut(s) | 505 |
| AoxI | GGCC | 2 cut(s) | 108, 154 |
| ApeKI | GCWGC | 4 cut(s) | 138, 141, 342, 487 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 3 cut(s) | 92, 231, 374 |
| AspS9I | GGNCC | 3 cut(s) | 154, 440, 534 |
| AsuC2I | CCSGG | 4 cut(s) | 107, 117, 153, 363 |
| AvaI | CYCGRG | 1 cut(s) | 505 |
| AvaII | GGWCC | 2 cut(s) | 440, 534 |
| BanI | GGYRCC | 3 cut(s) | 365, 379, 406 |
| BbvCI | CCTCAGC | 1 cut(s) | 243 |
| BbvI | GCAGC | 4 cut(s) | 150, 153, 354, 474 |
| BccI | CCATC | 3 cut(s) | 59, 430, 458 |
| BciT130I | CCWGG | 3 cut(s) | 135, 158, 349 |
| BcnI | CCSGG | 4 cut(s) | 107, 117, 153, 363 |
| BfaI | CTAG | 5 cut(s) | 182, 269, 302, 513, 579 |
| BfmI | CTRYAG | 2 cut(s) | 75, 525 |
| BfuAI | ACCTGC | 3 cut(s) | 377, 394, 522 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 6 cut(s) | 139, 142, 145, 343, 488, 491 |
| BlsI | GCNGC | 6 cut(s) | 140, 143, 146, 344, 489, 492 |
| Bme1390I | CCNGG | 7 cut(s) | 107, 117, 135, 153, 158, 349, 363 |
| Bme18I | GGWCC | 2 cut(s) | 440, 534 |
| BmeT110I | CYCGRG | 1 cut(s) | 505 |
| BmgT120I | GGNCC | 3 cut(s) | 154, 440, 534 |
| BmiI | GGNNCC | 5 cut(s) | 367, 381, 387, 408, 441 |
| BmrFI | CCNGG | 7 cut(s) | 107, 117, 135, 153, 158, 349, 363 |
| BmsI | GCATC | 3 cut(s) | 390, 558, 584 |
| Bpu10I | CCTNAGC | 1 cut(s) | 243 |
| BpuMI | CCSGG | 4 cut(s) | 107, 117, 153, 363 |
| BsaHI | GRCGYC | 1 cut(s) | 120 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 115 |
| BsaWI | WCCGGW | 1 cut(s) | 382 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 32, 62, 598 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 77, 151, 508 |
| Bse1I | ACTGG | 3 cut(s) | 192, 389, 608 |
| BseBI | CCWGG | 3 cut(s) | 135, 158, 349 |
| BseDI | CCNNGG | 2 cut(s) | 105, 115 |
| BseGI | GGATG | 4 cut(s) | 51, 441, 450, 599 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 77, 151, 508 |
| BseMII | CTCAG | 2 cut(s) | 234, 414 |
| BseNI | ACTGG | 3 cut(s) | 192, 389, 608 |
| BseRI | GAGGAG | 3 cut(s) | 155, 236, 239 |
| BseXI | GCAGC | 4 cut(s) | 150, 153, 354, 474 |
| BseYI | CCCAGC | 2 cut(s) | 70, 456 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 2 cut(s) | 110, 156 |
| BshNI | GGYRCC | 3 cut(s) | 365, 379, 406 |
| BsiHKCI | CYCGRG | 1 cut(s) | 505 |
| BsiSI | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 77, 151, 508 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsnI | GGCC | 2 cut(s) | 110, 156 |
| BsoBI | CYCGRG | 1 cut(s) | 505 |
| Bsp1407I | TGTACA | 1 cut(s) | 546 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspANI | GGCC | 2 cut(s) | 110, 156 |
| BspCNI | CTCAG | 2 cut(s) | 235, 415 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspLI | GGNNCC | 5 cut(s) | 367, 381, 387, 408, 441 |
| BspMAI | CTGCAG | 1 cut(s) | 529 |
| BspMI | ACCTGC | 3 cut(s) | 377, 394, 522 |
| BspPI | GGATC | 2 cut(s) | 168, 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 3 cut(s) | 365, 379, 406 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrGI | TGTACA | 1 cut(s) | 546 |
| BsrI | ACTGG | 3 cut(s) | 192, 389, 608 |
| BssECI | CCNNGG | 2 cut(s) | 105, 115 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| BssNI | GRCGYC | 1 cut(s) | 120 |
| Bst2UI | CCWGG | 3 cut(s) | 135, 158, 349 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 258, 336 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 287 |
| BstACI | GRCGYC | 1 cut(s) | 120 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 524 |
| BstAUI | TGTACA | 1 cut(s) | 546 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 149, 520, 529 |
| BstDEI | CTNAG | 2 cut(s) | 243, 423 |
| BstF5I | GGATG | 4 cut(s) | 51, 441, 450, 599 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstHHI | GCGC | 3 cut(s) | 92, 231, 374 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 409, 524, 528, 537 |
| BstNI | CCWGG | 3 cut(s) | 135, 158, 349 |
| BstSCI | CCNGG | 7 cut(s) | 105, 115, 133, 151, 156, 347, 361 |
| BstSFI | CTRYAG | 2 cut(s) | 75, 525 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 4 cut(s) | 150, 153, 354, 474 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 2 cut(s) | 110, 156 |
| BtgZI | GCGATG | 1 cut(s) | 581 |
| BtsCI | GGATG | 4 cut(s) | 51, 441, 450, 599 |
| BtsI | GCAGTG | 1 cut(s) | 522 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 522 |
| BveI | ACCTGC | 3 cut(s) | 377, 394, 522 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 149, 520, 529 |
| CfoI | GCGC | 3 cut(s) | 92, 231, 374 |
| Cfr13I | GGNCC | 3 cut(s) | 154, 440, 534 |
| CseI | GACGC | 3 cut(s) | 109, 307, 425 |
| Csp6I | GTAC | 4 cut(s) | 175, 380, 407, 547 |
| CviQI | GTAC | 4 cut(s) | 175, 380, 407, 547 |
| DdeI | CTNAG | 2 cut(s) | 243, 423 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 287 |
| EarI | CTCTTC | 2 cut(s) | 24, 287 |
| EciI | GGCGGA | 2 cut(s) | 206, 494 |
| Eco47I | GGWCC | 2 cut(s) | 440, 534 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 311 |
| Eco88I | CYCGRG | 1 cut(s) | 505 |
| EcoRII | CCWGG | 3 cut(s) | 133, 156, 347 |
| FaiI | YATR | 4 cut(s) | 173, 201, 395, 495 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FauI | CCCGC | 2 cut(s) | 8, 156 |
| Fnu4HI | GCNGC | 6 cut(s) | 139, 142, 145, 343, 488, 491 |
| FokI | GGATG | 4 cut(s) | 38, 437, 448, 606 |
| Fsp4HI | GCNGC | 6 cut(s) | 139, 142, 145, 343, 488, 491 |
| FspBI | CTAG | 5 cut(s) | 182, 269, 302, 513, 579 |
| FspI | TGCGCA | 1 cut(s) | 373 |
| GlaI | GCGC | 3 cut(s) | 91, 230, 373 |
| GluI | GCNGC | 6 cut(s) | 139, 142, 145, 343, 488, 491 |
| GsaI | CCCAGC | 2 cut(s) | 74, 460 |
| HaeIII | GGCC | 2 cut(s) | 110, 156 |
| HapII | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| HgaI | GACGC | 3 cut(s) | 109, 307, 425 |
| HhaI | GCGC | 3 cut(s) | 92, 231, 374 |
| Hin1I | GRCGYC | 1 cut(s) | 120 |
| Hin6I | GCGC | 3 cut(s) | 90, 229, 372 |
| HinP1I | GCGC | 3 cut(s) | 90, 229, 372 |
| HincII | GTYRAC | 3 cut(s) | 85, 261, 359 |
| HindII | GTYRAC | 3 cut(s) | 85, 261, 359 |
| HinfI | GANTC | 2 cut(s) | 482, 556 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 261, 359 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 261, 359 |
| Hpy99I | CGWCG | 2 cut(s) | 125, 565 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 258, 336 |
| HpyCH4IV | ACGT | 1 cut(s) | 217 |
| HpyCH4V | TGCA | 4 cut(s) | 403, 527, 571, 597 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 409, 524, 528, 537 |
| HpyF3I | CTNAG | 2 cut(s) | 243, 423 |
| HpySE526I | ACGT | 1 cut(s) | 217 |
| Hsp92I | GRCGYC | 1 cut(s) | 120 |
| HspAI | GCGC | 3 cut(s) | 90, 229, 372 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 3 cut(s) | 226, 385, 606 |
| Lsp1109I | GCAGC | 4 cut(s) | 150, 153, 354, 474 |
| LweI | GCATC | 3 cut(s) | 390, 558, 584 |
| MaeI | CTAG | 5 cut(s) | 182, 269, 302, 513, 579 |
| MaeII | ACGT | 1 cut(s) | 217 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 304 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 398 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 412, 540 |
| MspI | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| MspR9I | CCNGG | 7 cut(s) | 107, 117, 135, 153, 158, 349, 363 |
| MvaI | CCWGG | 3 cut(s) | 135, 158, 349 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 409, 524, 528, 537 |
| NciI | CCSGG | 4 cut(s) | 107, 117, 153, 363 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| NlaIV | GGNNCC | 5 cut(s) | 367, 381, 387, 408, 441 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| NsbI | TGCGCA | 1 cut(s) | 373 |
| PaqCI | CACCTGC | 1 cut(s) | 377 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 2 cut(s) | 482, 556 |
| PkrI | GCNGC | 6 cut(s) | 140, 143, 146, 344, 489, 492 |
| Psp6I | CCWGG | 3 cut(s) | 133, 156, 347 |
| PspFI | CCCAGC | 2 cut(s) | 70, 456 |
| PspGI | CCWGG | 3 cut(s) | 133, 156, 347 |
| PspN4I | GGNNCC | 5 cut(s) | 367, 381, 387, 408, 441 |
| PspPI | GGNCC | 3 cut(s) | 154, 440, 534 |
| PstI | CTGCAG | 1 cut(s) | 529 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuII | CAGCTG | 1 cut(s) | 74 |
| RsaI | GTAC | 4 cut(s) | 176, 381, 408, 548 |
| RsaNI | GTAC | 4 cut(s) | 175, 380, 407, 547 |
| RseI | CAYNNNNRTG | 1 cut(s) | 398 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 6 cut(s) | 139, 142, 145, 343, 488, 491 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 3 cut(s) | 154, 440, 534 |
| ScrFI | CCNGG | 7 cut(s) | 107, 117, 135, 153, 158, 349, 363 |
| SetI | ASST | 8 cut(s) | 76, 220, 371, 408, 424, 462, 536, 616 |
| SfaNI | GCATC | 3 cut(s) | 390, 558, 584 |
| SfcI | CTRYAG | 2 cut(s) | 75, 525 |
| SinI | GGWCC | 2 cut(s) | 440, 534 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 398 |
| SspMI | CTAG | 5 cut(s) | 182, 269, 302, 513, 579 |
| StyD4I | CCNGG | 7 cut(s) | 105, 115, 133, 151, 156, 347, 361 |
| TaaI | ACNGT | 3 cut(s) | 11, 258, 336 |
| TaiI | ACGT | 1 cut(s) | 220 |
| TaqI | TCGA | 1 cut(s) | 123 |
| TatI | WGTACW | 1 cut(s) | 546 |
| TauI | GCSGC | 2 cut(s) | 147, 493 |
| TfiI | GAWTC | 2 cut(s) | 482, 556 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 192, 529 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 4 cut(s) | 138, 141, 342, 487 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspDTI | ATGAA | 2 cut(s) | 188, 300 |
| TspGWI | ACGGA | 1 cut(s) | 549 |
| TspRI | CASTG | 2 cut(s) | 192, 529 |
| VpaK11BI | GGWCC | 2 cut(s) | 440, 534 |
| XspI | CTAG | 5 cut(s) | 182, 269, 302, 513, 579 |