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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 310 |
| Acc36I | ACCTGC | 1 cut(s) | 310 |
| AccI | GTMKAC | 1 cut(s) | 421 |
| AccII | CGCG | 1 cut(s) | 403 |
| AciI | CCGC | 6 cut(s) | 15, 74, 185, 231, 254, 403 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 1 cut(s) | 231 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 139, 265, 319, 431 |
| AjnI | CCWGG | 3 cut(s) | 147, 159, 219 |
| AluBI | AGCT | 2 cut(s) | 244, 408 |
| AluI | AGCT | 2 cut(s) | 244, 408 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 410 |
| Alw26I | GTCTC | 1 cut(s) | 302 |
| AlwI | GGATC | 1 cut(s) | 231 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 166 |
| AoxI | GGCC | 2 cut(s) | 303, 324 |
| ApeKI | GCWGC | 2 cut(s) | 182, 201 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AspLEI | GCGC | 2 cut(s) | 364, 388 |
| AspS9I | GGNCC | 3 cut(s) | 163, 217, 256 |
| AsuC2I | CCSGG | 1 cut(s) | 198 |
| AsuHPI | GGTGA | 1 cut(s) | 55 |
| AvaII | GGWCC | 3 cut(s) | 163, 217, 256 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanII | GRGCYC | 2 cut(s) | 155, 410 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 410 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 2 cut(s) | 194, 213 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 3 cut(s) | 149, 161, 221 |
| BcnI | CCSGG | 1 cut(s) | 198 |
| BcoDI | GTCTC | 1 cut(s) | 302 |
| BfaI | CTAG | 1 cut(s) | 135 |
| BfmI | CTRYAG | 1 cut(s) | 180 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BfuAI | ACCTGC | 1 cut(s) | 310 |
| BisI | GCNGC | 4 cut(s) | 75, 183, 202, 231 |
| BlsI | GCNGC | 4 cut(s) | 76, 184, 203, 232 |
| Bme1390I | CCNGG | 4 cut(s) | 149, 161, 198, 221 |
| Bme18I | GGWCC | 3 cut(s) | 163, 217, 256 |
| BmgT120I | GGNCC | 3 cut(s) | 163, 217, 256 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 4 cut(s) | 149, 161, 198, 221 |
| BpmI | CTGGAG | 2 cut(s) | 386, 394 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 198 |
| BsaI | GGTCTC | 1 cut(s) | 302 |
| BsaJI | CCNNGG | 3 cut(s) | 147, 148, 159 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 139, 265, 319, 431 |
| Bse118I | RCCGGY | 1 cut(s) | 431 |
| Bse1I | ACTGG | 1 cut(s) | 219 |
| BseBI | CCWGG | 3 cut(s) | 149, 161, 221 |
| BseDI | CCNNGG | 3 cut(s) | 147, 148, 159 |
| BseGI | GGATG | 1 cut(s) | 155 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 139, 265, 319, 431 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 1 cut(s) | 219 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 2 cut(s) | 194, 213 |
| BseYI | CCCAGC | 1 cut(s) | 382 |
| BsgI | GTGCAG | 1 cut(s) | 313 |
| Bsh1236I | CGCG | 1 cut(s) | 403 |
| Bsh1285I | CGRYCG | 1 cut(s) | 197 |
| BshFI | GGCC | 2 cut(s) | 305, 326 |
| BsiEI | CGRYCG | 1 cut(s) | 197 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 410 |
| BsiSI | CCGG | 3 cut(s) | 125, 197, 432 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 139, 265, 319, 431 |
| BsmAI | GTCTC | 1 cut(s) | 302 |
| BsnI | GGCC | 2 cut(s) | 305, 326 |
| Bso31I | GGTCTC | 1 cut(s) | 302 |
| Bsp1286I | GDGCHC | 3 cut(s) | 112, 155, 410 |
| Bsp143I | GATC | 2 cut(s) | 40, 223 |
| BspACI | CCGC | 6 cut(s) | 15, 74, 185, 231, 254, 403 |
| BspANI | GGCC | 2 cut(s) | 305, 326 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 1 cut(s) | 403 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspMAI | CTGCAG | 1 cut(s) | 184 |
| BspMI | ACCTGC | 1 cut(s) | 310 |
| BspPI | GGATC | 1 cut(s) | 231 |
| BspTNI | GGTCTC | 1 cut(s) | 302 |
| BsrFI | RCCGGY | 1 cut(s) | 431 |
| BsrI | ACTGG | 1 cut(s) | 219 |
| BssAI | RCCGGY | 1 cut(s) | 431 |
| BssECI | CCNNGG | 3 cut(s) | 147, 148, 159 |
| BssMI | GATC | 2 cut(s) | 40, 223 |
| Bst2UI | CCWGG | 3 cut(s) | 149, 161, 221 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 94 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 246, 395 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 1 cut(s) | 155 |
| BstFNI | CGCG | 1 cut(s) | 403 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 2 cut(s) | 364, 388 |
| BstKTI | GATC | 2 cut(s) | 43, 226 |
| BstMAI | GTCTC | 1 cut(s) | 302 |
| BstMBI | GATC | 2 cut(s) | 40, 223 |
| BstMCI | CGRYCG | 1 cut(s) | 197 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 188, 207, 368, 383, 394 |
| BstNI | CCWGG | 3 cut(s) | 149, 161, 221 |
| BstSCI | CCNGG | 4 cut(s) | 147, 159, 196, 219 |
| BstSFI | CTRYAG | 1 cut(s) | 180 |
| BstUI | CGCG | 1 cut(s) | 403 |
| BstV1I | GCAGC | 2 cut(s) | 194, 213 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| BsuRI | GGCC | 2 cut(s) | 305, 326 |
| BtsCI | GGATG | 1 cut(s) | 155 |
| BtsI | GCAGTG | 1 cut(s) | 255 |
| BtsIMutI | CAGTG | 3 cut(s) | 212, 255, 329 |
| BveI | ACCTGC | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 246, 395 |
| CaiI | CAGNNNCTG | 1 cut(s) | 166 |
| CfoI | GCGC | 2 cut(s) | 364, 388 |
| Cfr10I | RCCGGY | 1 cut(s) | 431 |
| Cfr13I | GGNCC | 3 cut(s) | 163, 217, 256 |
| CviJI | RGCY | 7 cut(s) | 77, 153, 244, 305, 326, 408, 435 |
| CviKI_1 | RGCY | 7 cut(s) | 77, 153, 244, 305, 326, 408, 435 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 225 |
| DpnII | GATC | 2 cut(s) | 40, 223 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| EciI | GGCGGA | 1 cut(s) | 269 |
| Ecl136II | GAGCTC | 1 cut(s) | 408 |
| Eco24I | GRGCYC | 2 cut(s) | 155, 410 |
| Eco31I | GGTCTC | 1 cut(s) | 302 |
| Eco47I | GGWCC | 3 cut(s) | 163, 217, 256 |
| Eco53kI | GAGCTC | 1 cut(s) | 408 |
| EcoICRI | GAGCTC | 1 cut(s) | 408 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 163 |
| EcoRII | CCWGG | 3 cut(s) | 147, 159, 219 |
| EcoT38I | GRGCYC | 2 cut(s) | 155, 410 |
| FaiI | YATR | 2 cut(s) | 236, 240 |
| FauI | CCCGC | 1 cut(s) | 8 |
| FblI | GTMKAC | 1 cut(s) | 421 |
| Fnu4HI | GCNGC | 4 cut(s) | 75, 183, 202, 231 |
| FokI | GGATG | 1 cut(s) | 142 |
| FriOI | GRGCYC | 2 cut(s) | 155, 410 |
| Fsp4HI | GCNGC | 4 cut(s) | 75, 183, 202, 231 |
| FspBI | CTAG | 1 cut(s) | 135 |
| GlaI | GCGC | 2 cut(s) | 363, 387 |
| GluI | GCNGC | 4 cut(s) | 75, 183, 202, 231 |
| GsaI | CCCAGC | 1 cut(s) | 386 |
| GsuI | CTGGAG | 2 cut(s) | 386, 394 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 2 cut(s) | 305, 326 |
| HapII | CCGG | 3 cut(s) | 125, 197, 432 |
| HhaI | GCGC | 2 cut(s) | 364, 388 |
| Hin6I | GCGC | 2 cut(s) | 362, 386 |
| HinP1I | GCGC | 2 cut(s) | 362, 386 |
| HincII | GTYRAC | 4 cut(s) | 97, 103, 130, 422 |
| HindII | GTYRAC | 4 cut(s) | 97, 103, 130, 422 |
| HpaI | GTTAAC | 2 cut(s) | 97, 103 |
| HpaII | CCGG | 3 cut(s) | 125, 197, 432 |
| HphI | GGTGA | 1 cut(s) | 55 |
| Hpy166II | GTNNAC | 4 cut(s) | 97, 103, 130, 422 |
| Hpy188III | TCNNGA | 1 cut(s) | 411 |
| Hpy8I | GTNNAC | 4 cut(s) | 97, 103, 130, 422 |
| Hpy99I | CGWCG | 1 cut(s) | 350 |
| HpyAV | CCTTC | 1 cut(s) | 295 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 94 |
| HpyCH4IV | ACGT | 2 cut(s) | 99, 415 |
| HpyCH4V | TGCA | 2 cut(s) | 182, 294 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 188, 207, 368, 383, 394 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 2 cut(s) | 99, 415 |
| HspAI | GCGC | 2 cut(s) | 362, 386 |
| KspAI | GTTAAC | 2 cut(s) | 97, 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 223 |
| LmnI | GCTCC | 3 cut(s) | 31, 405, 413 |
| Lsp1109I | GCAGC | 2 cut(s) | 194, 213 |
| MaeI | CTAG | 1 cut(s) | 135 |
| MaeII | ACGT | 2 cut(s) | 99, 415 |
| MalI | GATC | 2 cut(s) | 42, 225 |
| MboI | GATC | 2 cut(s) | 40, 223 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 3 cut(s) | 112, 155, 410 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 1 cut(s) | 341 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MnlI | CCTC | 4 cut(s) | 22, 181, 269, 361 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 185 |
| MspI | CCGG | 3 cut(s) | 125, 197, 432 |
| MspR9I | CCNGG | 4 cut(s) | 149, 161, 198, 221 |
| MvaI | CCWGG | 3 cut(s) | 149, 161, 221 |
| MvnI | CGCG | 1 cut(s) | 403 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 188, 207, 368, 383, 394 |
| NciI | CCSGG | 1 cut(s) | 198 |
| NdeII | GATC | 2 cut(s) | 40, 223 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NmeAIII | GCCGAG | 1 cut(s) | 331 |
| PaqCI | CACCTGC | 1 cut(s) | 310 |
| PasI | CCCWGGG | 1 cut(s) | 148 |
| PflFI | GACNNNGTC | 1 cut(s) | 417 |
| PkrI | GCNGC | 4 cut(s) | 76, 184, 203, 232 |
| PpuMI | RGGWCCY | 1 cut(s) | 163 |
| Psp124BI | GAGCTC | 1 cut(s) | 410 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 163 |
| Psp6I | CCWGG | 3 cut(s) | 147, 159, 219 |
| PspFI | CCCAGC | 1 cut(s) | 382 |
| PspGI | CCWGG | 3 cut(s) | 147, 159, 219 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 3 cut(s) | 163, 217, 256 |
| PspPPI | RGGWCCY | 1 cut(s) | 163 |
| PstI | CTGCAG | 1 cut(s) | 184 |
| PstNI | CAGNNNCTG | 1 cut(s) | 166 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| PsyI | GACNNNGTC | 1 cut(s) | 417 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SacI | GAGCTC | 1 cut(s) | 410 |
| SalI | GTCGAC | 1 cut(s) | 420 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 4 cut(s) | 75, 183, 202, 231 |
| Sau3AI | GATC | 2 cut(s) | 40, 223 |
| Sau96I | GGNCC | 3 cut(s) | 163, 217, 256 |
| ScrFI | CCNGG | 4 cut(s) | 149, 161, 198, 221 |
| SduI | GDGCHC | 3 cut(s) | 112, 155, 410 |
| SetI | ASST | 8 cut(s) | 72, 102, 165, 246, 261, 324, 410, 418 |
| SfcI | CTRYAG | 1 cut(s) | 180 |
| SinI | GGWCC | 3 cut(s) | 163, 217, 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| Sse9I | AATT | 1 cut(s) | 341 |
| SsiI | CCGC | 6 cut(s) | 15, 74, 185, 231, 254, 403 |
| SspMI | CTAG | 1 cut(s) | 135 |
| SstI | GAGCTC | 1 cut(s) | 410 |
| StyD4I | CCNGG | 4 cut(s) | 147, 159, 196, 219 |
| TaaI | ACNGT | 2 cut(s) | 11, 94 |
| TaiI | ACGT | 2 cut(s) | 102, 418 |
| TaqI | TCGA | 2 cut(s) | 345, 421 |
| TasI | AATT | 1 cut(s) | 341 |
| TauI | GCSGC | 2 cut(s) | 77, 233 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TscAI | CASTG | 3 cut(s) | 219, 255, 336 |
| TseI | GCWGC | 2 cut(s) | 182, 201 |
| TspRI | CASTG | 3 cut(s) | 219, 255, 336 |
| Tth111I | GACNNNGTC | 1 cut(s) | 417 |
| VpaK11BI | GGWCC | 3 cut(s) | 163, 217, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 1 cut(s) | 341 |
| XmiI | GTMKAC | 1 cut(s) | 421 |
| XspI | CTAG | 1 cut(s) | 135 |