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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 328 |
| Acc36I | ACCTGC | 1 cut(s) | 520 |
| Acc65I | GGTACC | 1 cut(s) | 625 |
| AccB1I | GGYRCC | 3 cut(s) | 191, 625, 729 |
| AccII | CGCG | 1 cut(s) | 99 |
| AclWI | GGATC | 1 cut(s) | 225 |
| AcsI | RAATTY | 2 cut(s) | 335, 790 |
| AfaI | GTAC | 2 cut(s) | 522, 627 |
| AgsI | TTSAA | 2 cut(s) | 29, 472 |
| AjnI | CCWGG | 3 cut(s) | 208, 644, 770 |
| AluBI | AGCT | 4 cut(s) | 242, 305, 346, 716 |
| AluI | AGCT | 4 cut(s) | 242, 305, 346, 716 |
| Alw21I | GWGCWC | 1 cut(s) | 159 |
| AlwI | GGATC | 1 cut(s) | 225 |
| Ama87I | CYCGRG | 1 cut(s) | 149 |
| AoxI | GGCC | 3 cut(s) | 647, 724, 773 |
| ApeKI | GCWGC | 1 cut(s) | 434 |
| ApoI | RAATTY | 2 cut(s) | 335, 790 |
| Asp718I | GGTACC | 1 cut(s) | 625 |
| AspLEI | GCGC | 2 cut(s) | 404, 505 |
| AsuNHI | GCTAGC | 1 cut(s) | 5 |
| AvaI | CYCGRG | 1 cut(s) | 149 |
| BanI | GGYRCC | 3 cut(s) | 191, 625, 729 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 690, 722 |
| Bbv12I | GWGCWC | 1 cut(s) | 159 |
| BbvCI | CCTCAGC | 1 cut(s) | 57 |
| BbvI | GCAGC | 1 cut(s) | 446 |
| BccI | CCATC | 1 cut(s) | 207 |
| BciT130I | CCWGG | 3 cut(s) | 210, 646, 772 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BfoI | RGCGCY | 1 cut(s) | 506 |
| BfuAI | ACCTGC | 1 cut(s) | 520 |
| BisI | GCNGC | 4 cut(s) | 16, 225, 435, 548 |
| BlpI | GCTNAGC | 1 cut(s) | 410 |
| BlsI | GCNGC | 4 cut(s) | 17, 226, 436, 549 |
| Bme1390I | CCNGG | 3 cut(s) | 210, 646, 772 |
| BmeT110I | CYCGRG | 1 cut(s) | 149 |
| BmiI | GGNNCC | 3 cut(s) | 193, 627, 731 |
| BmrFI | CCNGG | 3 cut(s) | 210, 646, 772 |
| BmtI | GCTAGC | 1 cut(s) | 9 |
| Bpu10I | CCTNAGC | 1 cut(s) | 57 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 410 |
| BsaJI | CCNNGG | 3 cut(s) | 150, 174, 644 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 591, 621 |
| Bse118I | RCCGGY | 2 cut(s) | 188, 726 |
| Bse1I | ACTGG | 1 cut(s) | 446 |
| Bse3DI | GCAATG | 1 cut(s) | 25 |
| BseBI | CCWGG | 3 cut(s) | 210, 646, 772 |
| BseDI | CCNNGG | 3 cut(s) | 150, 174, 644 |
| BseGI | GGATG | 2 cut(s) | 218, 373 |
| BseMI | GCAATG | 1 cut(s) | 25 |
| BseMII | CTCAG | 2 cut(s) | 71, 424 |
| BseNI | ACTGG | 1 cut(s) | 446 |
| BseXI | GCAGC | 1 cut(s) | 446 |
| Bsh1236I | CGCG | 1 cut(s) | 99 |
| BshFI | GGCC | 3 cut(s) | 649, 726, 775 |
| BshNI | GGYRCC | 3 cut(s) | 191, 625, 729 |
| BsiHKAI | GWGCWC | 1 cut(s) | 159 |
| BsiHKCI | CYCGRG | 1 cut(s) | 149 |
| BsiSI | CCGG | 2 cut(s) | 189, 727 |
| BsnI | GGCC | 3 cut(s) | 649, 726, 775 |
| BsoBI | CYCGRG | 1 cut(s) | 149 |
| Bsp1286I | GDGCHC | 1 cut(s) | 159 |
| Bsp143I | GATC | 2 cut(s) | 31, 217 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 410 |
| Bsp19I | CCATGG | 1 cut(s) | 174 |
| BspANI | GGCC | 3 cut(s) | 649, 726, 775 |
| BspCNI | CTCAG | 2 cut(s) | 70, 423 |
| BspFNI | CGCG | 1 cut(s) | 99 |
| BspLI | GGNNCC | 3 cut(s) | 193, 627, 731 |
| BspMI | ACCTGC | 1 cut(s) | 520 |
| BspOI | GCTAGC | 1 cut(s) | 9 |
| BspPI | GGATC | 1 cut(s) | 225 |
| BspT107I | GGYRCC | 3 cut(s) | 191, 625, 729 |
| BsrDI | GCAATG | 1 cut(s) | 25 |
| BsrFI | RCCGGY | 2 cut(s) | 188, 726 |
| BsrI | ACTGG | 1 cut(s) | 446 |
| BssAI | RCCGGY | 2 cut(s) | 188, 726 |
| BssECI | CCNNGG | 3 cut(s) | 150, 174, 644 |
| BssMI | GATC | 2 cut(s) | 31, 217 |
| BssT1I | CCWWGG | 1 cut(s) | 174 |
| Bst2UI | CCWGG | 3 cut(s) | 210, 646, 772 |
| Bst4CI | ACNGT | 4 cut(s) | 91, 131, 655, 803 |
| BstC8I | GCNNGC | 4 cut(s) | 7, 574, 578, 728 |
| BstDEI | CTNAG | 3 cut(s) | 57, 410, 710 |
| BstDSI | CCRYGG | 1 cut(s) | 174 |
| BstF5I | GGATG | 2 cut(s) | 218, 373 |
| BstFNI | CGCG | 1 cut(s) | 99 |
| BstH2I | RGCGCY | 1 cut(s) | 506 |
| BstHHI | GCGC | 2 cut(s) | 404, 505 |
| BstKTI | GATC | 2 cut(s) | 34, 220 |
| BstMBI | GATC | 2 cut(s) | 31, 217 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 15, 80, 377 |
| BstNI | CCWGG | 3 cut(s) | 210, 646, 772 |
| BstSCI | CCNGG | 3 cut(s) | 208, 644, 770 |
| BstUI | CGCG | 1 cut(s) | 99 |
| BstV1I | GCAGC | 1 cut(s) | 446 |
| BstX2I | RGATCY | 1 cut(s) | 217 |
| BstYI | RGATCY | 1 cut(s) | 217 |
| BsuRI | GGCC | 3 cut(s) | 649, 726, 775 |
| BtgI | CCRYGG | 1 cut(s) | 174 |
| BtsCI | GGATG | 2 cut(s) | 218, 373 |
| BtsIMutI | CAGTG | 1 cut(s) | 574 |
| BveI | ACCTGC | 1 cut(s) | 520 |
| Cac8I | GCNNGC | 4 cut(s) | 7, 574, 578, 728 |
| CfoI | GCGC | 2 cut(s) | 404, 505 |
| Cfr10I | RCCGGY | 2 cut(s) | 188, 726 |
| CseI | GACGC | 1 cut(s) | 668 |
| Csp6I | GTAC | 2 cut(s) | 521, 626 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 465, 500 |
| CviAII | CATG | 2 cut(s) | 175, 229 |
| CviQI | GTAC | 2 cut(s) | 521, 626 |
| DdeI | CTNAG | 3 cut(s) | 57, 410, 710 |
| DpnI | GATC | 2 cut(s) | 33, 219 |
| DpnII | GATC | 2 cut(s) | 31, 217 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 328 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 328 |
| EciI | GGCGGA | 1 cut(s) | 263 |
| Eco130I | CCWWGG | 1 cut(s) | 174 |
| Eco147I | AGGCCT | 2 cut(s) | 649, 775 |
| Eco88I | CYCGRG | 1 cut(s) | 149 |
| EcoRII | CCWGG | 3 cut(s) | 208, 644, 770 |
| EcoT14I | CCWWGG | 1 cut(s) | 174 |
| ErhI | CCWWGG | 1 cut(s) | 174 |
| FaeI | CATG | 2 cut(s) | 178, 232 |
| FaiI | YATR | 4 cut(s) | 176, 230, 464, 599 |
| FatI | CATG | 2 cut(s) | 174, 228 |
| FauI | CCCGC | 1 cut(s) | 681 |
| Fnu4HI | GCNGC | 4 cut(s) | 16, 225, 435, 548 |
| FokI | GGATG | 2 cut(s) | 225, 360 |
| Fsp4HI | GCNGC | 4 cut(s) | 16, 225, 435, 548 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GlaI | GCGC | 2 cut(s) | 403, 504 |
| GluI | GCNGC | 4 cut(s) | 16, 225, 435, 548 |
| HaeII | RGCGCY | 1 cut(s) | 506 |
| HaeIII | GGCC | 3 cut(s) | 649, 726, 775 |
| HapII | CCGG | 2 cut(s) | 189, 727 |
| HgaI | GACGC | 1 cut(s) | 668 |
| HhaI | GCGC | 2 cut(s) | 404, 505 |
| Hin1II | CATG | 2 cut(s) | 178, 232 |
| Hin6I | GCGC | 2 cut(s) | 402, 503 |
| HinP1I | GCGC | 2 cut(s) | 402, 503 |
| HincII | GTYRAC | 3 cut(s) | 52, 94, 658 |
| HindII | GTYRAC | 3 cut(s) | 52, 94, 658 |
| HinfI | GANTC | 2 cut(s) | 472, 539 |
| HpaII | CCGG | 2 cut(s) | 189, 727 |
| Hpy166II | GTNNAC | 4 cut(s) | 52, 94, 108, 658 |
| Hpy188I | TCNGA | 2 cut(s) | 36, 611 |
| Hpy8I | GTNNAC | 4 cut(s) | 52, 94, 108, 658 |
| HpyAV | CCTTC | 1 cut(s) | 289 |
| HpyCH4III | ACNGT | 4 cut(s) | 91, 131, 655, 803 |
| HpyCH4V | TGCA | 2 cut(s) | 256, 797 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 15, 80, 377 |
| HpyF3I | CTNAG | 3 cut(s) | 57, 410, 710 |
| Hsp92II | CATG | 2 cut(s) | 178, 232 |
| HspAI | GCGC | 2 cut(s) | 402, 503 |
| KpnI | GGTACC | 1 cut(s) | 629 |
| KroI | GCCGGC | 1 cut(s) | 726 |
| KroNI | GCCGGC | 1 cut(s) | 728 |
| Kzo9I | GATC | 2 cut(s) | 31, 217 |
| LmnI | GCTCC | 1 cut(s) | 162 |
| Lsp1109I | GCAGC | 1 cut(s) | 446 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeIII | GTNAC | 1 cut(s) | 705 |
| MalI | GATC | 2 cut(s) | 33, 219 |
| MboI | GATC | 2 cut(s) | 31, 217 |
| MboII | GAAGA | 2 cut(s) | 41, 710 |
| MfeI | CAATTG | 1 cut(s) | 515 |
| MflI | RGATCY | 1 cut(s) | 217 |
| MhlI | GDGCHC | 1 cut(s) | 159 |
| MluCI | AATT | 8 cut(s) | 44, 101, 117, 335, 482, 515, 692, 790 |
| MlyI | GAGTC | 1 cut(s) | 533 |
| MmeI | TCCRAC | 2 cut(s) | 213, 634 |
| MnlI | CCTC | 7 cut(s) | 66, 145, 149, 578, 617, 648, 686 |
| MroNI | GCCGGC | 1 cut(s) | 726 |
| MseI | TTAA | 1 cut(s) | 120 |
| MslI | CAYNNNNRTG | 1 cut(s) | 211 |
| MspI | CCGG | 2 cut(s) | 189, 727 |
| MspR9I | CCNGG | 3 cut(s) | 210, 646, 772 |
| MunI | CAATTG | 1 cut(s) | 515 |
| MvaI | CCWGG | 3 cut(s) | 210, 646, 772 |
| MvnI | CGCG | 1 cut(s) | 99 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 15, 80, 377 |
| NaeI | GCCGGC | 1 cut(s) | 728 |
| NcoI | CCATGG | 1 cut(s) | 174 |
| NdeII | GATC | 2 cut(s) | 31, 217 |
| NgoMIV | GCCGGC | 1 cut(s) | 726 |
| NheI | GCTAGC | 1 cut(s) | 5 |
| NlaIII | CATG | 2 cut(s) | 178, 232 |
| NlaIV | GGNNCC | 3 cut(s) | 193, 627, 731 |
| PceI | AGGCCT | 2 cut(s) | 649, 775 |
| PdiI | GCCGGC | 1 cut(s) | 728 |
| PfeI | GAWTC | 1 cut(s) | 472 |
| PkrI | GCNGC | 4 cut(s) | 17, 226, 436, 549 |
| PleI | GAGTC | 1 cut(s) | 533 |
| PpsI | GAGTC | 1 cut(s) | 533 |
| Psp6I | CCWGG | 3 cut(s) | 208, 644, 770 |
| PspGI | CCWGG | 3 cut(s) | 208, 644, 770 |
| PspN4I | GGNNCC | 3 cut(s) | 193, 627, 731 |
| PsuI | RGATCY | 1 cut(s) | 217 |
| RsaI | GTAC | 2 cut(s) | 522, 627 |
| RsaNI | GTAC | 2 cut(s) | 521, 626 |
| RseI | CAYNNNNRTG | 1 cut(s) | 211 |
| SaqAI | TTAA | 1 cut(s) | 120 |
| SatI | GCNGC | 4 cut(s) | 16, 225, 435, 548 |
| Sau3AI | GATC | 2 cut(s) | 31, 217 |
| SchI | GAGTC | 1 cut(s) | 533 |
| ScrFI | CCNGG | 3 cut(s) | 210, 646, 772 |
| SduI | GDGCHC | 1 cut(s) | 159 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 211 |
| Sse9I | AATT | 8 cut(s) | 44, 101, 117, 335, 482, 515, 692, 790 |
| SseBI | AGGCCT | 2 cut(s) | 649, 775 |
| SspMI | CTAG | 1 cut(s) | 6 |
| StuI | AGGCCT | 2 cut(s) | 649, 775 |
| StyD4I | CCNGG | 3 cut(s) | 208, 644, 770 |
| StyI | CCWWGG | 1 cut(s) | 174 |
| TaaI | ACNGT | 4 cut(s) | 91, 131, 655, 803 |
| TaqI | TCGA | 1 cut(s) | 339 |
| TasI | AATT | 8 cut(s) | 44, 101, 117, 335, 482, 515, 692, 790 |
| TauI | GCSGC | 3 cut(s) | 18, 227, 550 |
| TfiI | GAWTC | 1 cut(s) | 472 |
| Tru1I | TTAA | 1 cut(s) | 120 |
| Tru9I | TTAA | 1 cut(s) | 120 |
| TscAI | CASTG | 1 cut(s) | 574 |
| TseI | GCWGC | 1 cut(s) | 434 |
| TspRI | CASTG | 1 cut(s) | 574 |
| XapI | RAATTY | 2 cut(s) | 335, 790 |
| XspI | CTAG | 1 cut(s) | 6 |