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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 223 |
| Acc65I | GGTACC | 2 cut(s) | 208, 235 |
| AccB1I | GGYRCC | 2 cut(s) | 208, 235 |
| AccBSI | CCGCTC | 1 cut(s) | 333 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AciI | CCGC | 5 cut(s) | 20, 141, 275, 320, 331 |
| AfaI | GTAC | 3 cut(s) | 5, 210, 237 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 31, 85, 197 |
| AjnI | CCWGG | 1 cut(s) | 176 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 130, 162 |
| AluBI | AGCT | 3 cut(s) | 10, 289, 341 |
| AluI | AGCT | 3 cut(s) | 10, 289, 341 |
| AoxI | GGCC | 1 cut(s) | 75 |
| ApeKI | GCWGC | 1 cut(s) | 171 |
| Asp718I | GGTACC | 2 cut(s) | 208, 235 |
| AspLEI | GCGC | 2 cut(s) | 154, 373 |
| AspS9I | GGNCC | 5 cut(s) | 22, 76, 244, 269, 351 |
| AvaII | GGWCC | 4 cut(s) | 22, 244, 269, 351 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 192, 225 |
| BanI | GGYRCC | 2 cut(s) | 208, 235 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BbvI | GCAGC | 1 cut(s) | 183 |
| BccI | CCATC | 2 cut(s) | 259, 287 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 298, 332 |
| BciT130I | CCWGG | 1 cut(s) | 178 |
| BfaI | CTAG | 2 cut(s) | 213, 342 |
| BfuAI | ACCTGC | 1 cut(s) | 223 |
| BisI | GCNGC | 2 cut(s) | 172, 320 |
| BlsI | GCNGC | 2 cut(s) | 173, 321 |
| Bme1390I | CCNGG | 1 cut(s) | 178 |
| Bme18I | GGWCC | 4 cut(s) | 22, 244, 269, 351 |
| BmgT120I | GGNCC | 5 cut(s) | 22, 76, 244, 269, 351 |
| BmiI | GGNNCC | 4 cut(s) | 210, 237, 246, 270 |
| BmrFI | CCNGG | 1 cut(s) | 178 |
| BmsI | GCATC | 1 cut(s) | 219 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| BsaJI | CCNNGG | 2 cut(s) | 354, 374 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 31, 85, 197 |
| Bse1I | ACTGG | 3 cut(s) | 21, 202, 218 |
| BseBI | CCWGG | 1 cut(s) | 178 |
| BseDI | CCNNGG | 2 cut(s) | 354, 374 |
| BseGI | GGATG | 2 cut(s) | 270, 279 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 31, 85, 197 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseNI | ACTGG | 3 cut(s) | 21, 202, 218 |
| BseRI | GAGGAG | 1 cut(s) | 68 |
| BseXI | GCAGC | 1 cut(s) | 183 |
| BseYI | CCCAGC | 1 cut(s) | 285 |
| BshFI | GGCC | 1 cut(s) | 77 |
| BshNI | GGYRCC | 2 cut(s) | 208, 235 |
| BslFI | GGGAC | 1 cut(s) | 282 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 31, 85, 197 |
| BsmFI | GGGAC | 1 cut(s) | 282 |
| BsnI | GGCC | 1 cut(s) | 77 |
| BspACI | CCGC | 5 cut(s) | 20, 141, 275, 320, 331 |
| BspANI | GGCC | 1 cut(s) | 77 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspLI | GGNNCC | 4 cut(s) | 210, 237, 246, 270 |
| BspMI | ACCTGC | 1 cut(s) | 223 |
| BspT107I | GGYRCC | 2 cut(s) | 208, 235 |
| BsrBI | CCGCTC | 1 cut(s) | 333 |
| BsrI | ACTGG | 3 cut(s) | 21, 202, 218 |
| BssECI | CCNNGG | 2 cut(s) | 354, 374 |
| BssT1I | CCWWGG | 1 cut(s) | 374 |
| Bst2UI | CCWGG | 1 cut(s) | 178 |
| Bst4CI | ACNGT | 1 cut(s) | 240 |
| Bst6I | CTCTTC | 1 cut(s) | 45 |
| BstC8I | GCNNGC | 3 cut(s) | 12, 161, 369 |
| BstDEI | CTNAG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstF5I | GGATG | 2 cut(s) | 270, 279 |
| BstHHI | GCGC | 2 cut(s) | 154, 373 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 149, 160 |
| BstNI | CCWGG | 1 cut(s) | 178 |
| BstSCI | CCNGG | 1 cut(s) | 176 |
| BstV1I | GCAGC | 1 cut(s) | 183 |
| BsuRI | GGCC | 1 cut(s) | 77 |
| BtsCI | GGATG | 2 cut(s) | 270, 279 |
| BtsIMutI | CAGTG | 3 cut(s) | 14, 195, 321 |
| BveI | ACCTGC | 1 cut(s) | 223 |
| Cac8I | GCNNGC | 3 cut(s) | 12, 161, 369 |
| CfoI | GCGC | 2 cut(s) | 154, 373 |
| Cfr13I | GGNCC | 5 cut(s) | 22, 76, 244, 269, 351 |
| CseI | GACGC | 1 cut(s) | 136 |
| Csp6I | GTAC | 3 cut(s) | 4, 209, 236 |
| CviJI | RGCY | 6 cut(s) | 10, 71, 77, 92, 289, 341 |
| CviKI_1 | RGCY | 6 cut(s) | 10, 71, 77, 92, 289, 341 |
| CviQI | GTAC | 3 cut(s) | 4, 209, 236 |
| DdeI | CTNAG | 1 cut(s) | 72 |
| Eam1104I | CTCTTC | 1 cut(s) | 45 |
| EarI | CTCTTC | 1 cut(s) | 45 |
| EciI | GGCGGA | 1 cut(s) | 35 |
| Eco130I | CCWWGG | 1 cut(s) | 374 |
| Eco47I | GGWCC | 4 cut(s) | 22, 244, 269, 351 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoRII | CCWGG | 1 cut(s) | 176 |
| EcoT14I | CCWWGG | 1 cut(s) | 374 |
| ErhI | CCWWGG | 1 cut(s) | 374 |
| FaiI | YATR | 1 cut(s) | 224 |
| FaqI | GGGAC | 1 cut(s) | 282 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 2 cut(s) | 172, 320 |
| FokI | GGATG | 2 cut(s) | 266, 277 |
| Fsp4HI | GCNGC | 2 cut(s) | 172, 320 |
| FspBI | CTAG | 2 cut(s) | 213, 342 |
| GlaI | GCGC | 2 cut(s) | 153, 372 |
| GluI | GCNGC | 2 cut(s) | 172, 320 |
| GsaI | CCCAGC | 1 cut(s) | 289 |
| HaeIII | GGCC | 1 cut(s) | 77 |
| HgaI | GACGC | 1 cut(s) | 136 |
| HhaI | GCGC | 2 cut(s) | 154, 373 |
| Hin6I | GCGC | 2 cut(s) | 152, 371 |
| HinP1I | GCGC | 2 cut(s) | 152, 371 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| Hpy166II | GTNNAC | 1 cut(s) | 188 |
| Hpy188I | TCNGA | 1 cut(s) | 337 |
| Hpy8I | GTNNAC | 1 cut(s) | 188 |
| HpyAV | CCTTC | 2 cut(s) | 35, 61 |
| HpyCH4III | ACNGT | 1 cut(s) | 240 |
| HpyCH4V | TGCA | 1 cut(s) | 232 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 149, 160 |
| HpyF3I | CTNAG | 1 cut(s) | 72 |
| HspAI | GCGC | 2 cut(s) | 152, 371 |
| KpnI | GGTACC | 2 cut(s) | 212, 239 |
| LmnI | GCTCC | 2 cut(s) | 55, 298 |
| Lsp1109I | GCAGC | 1 cut(s) | 183 |
| LweI | GCATC | 1 cut(s) | 219 |
| MaeI | CTAG | 2 cut(s) | 213, 342 |
| MaeIII | GTNAC | 1 cut(s) | 102 |
| MbiI | CCGCTC | 1 cut(s) | 333 |
| MboII | GAAGA | 1 cut(s) | 62 |
| MmeI | TCCRAC | 2 cut(s) | 222, 276 |
| MnlI | CCTC | 5 cut(s) | 46, 67, 298, 342, 349 |
| MslI | CAYNNNNRTG | 1 cut(s) | 227 |
| MspR9I | CCNGG | 1 cut(s) | 178 |
| MvaI | CCWGG | 1 cut(s) | 178 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 149, 160 |
| NlaIV | GGNNCC | 4 cut(s) | 210, 237, 246, 270 |
| NmuCI | GTSAC | 1 cut(s) | 102 |
| PkrI | GCNGC | 2 cut(s) | 173, 321 |
| Psp6I | CCWGG | 1 cut(s) | 176 |
| PspFI | CCCAGC | 1 cut(s) | 285 |
| PspGI | CCWGG | 1 cut(s) | 176 |
| PspN4I | GGNNCC | 4 cut(s) | 210, 237, 246, 270 |
| PspPI | GGNCC | 5 cut(s) | 22, 76, 244, 269, 351 |
| RsaI | GTAC | 3 cut(s) | 5, 210, 237 |
| RsaNI | GTAC | 3 cut(s) | 4, 209, 236 |
| RseI | CAYNNNNRTG | 1 cut(s) | 227 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SatI | GCNGC | 2 cut(s) | 172, 320 |
| Sau96I | GGNCC | 5 cut(s) | 22, 76, 244, 269, 351 |
| ScrFI | CCNGG | 1 cut(s) | 178 |
| SetI | ASST | 8 cut(s) | 12, 27, 90, 214, 237, 253, 291, 343 |
| SfaNI | GCATC | 1 cut(s) | 219 |
| SinI | GGWCC | 4 cut(s) | 22, 244, 269, 351 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 227 |
| SsiI | CCGC | 5 cut(s) | 20, 141, 275, 320, 331 |
| SspMI | CTAG | 2 cut(s) | 213, 342 |
| StyD4I | CCNGG | 1 cut(s) | 176 |
| StyI | CCWWGG | 1 cut(s) | 374 |
| TaaI | ACNGT | 1 cut(s) | 240 |
| TaqI | TCGA | 1 cut(s) | 187 |
| TaqII | GACCGA | 1 cut(s) | 368 |
| TauI | GCSGC | 1 cut(s) | 322 |
| TscAI | CASTG | 3 cut(s) | 21, 202, 321 |
| TseFI | GTSAC | 1 cut(s) | 102 |
| TseI | GCWGC | 1 cut(s) | 171 |
| Tsp45I | GTSAC | 1 cut(s) | 102 |
| TspRI | CASTG | 3 cut(s) | 21, 202, 321 |
| VpaK11BI | GGWCC | 4 cut(s) | 22, 244, 269, 351 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 2 cut(s) | 213, 342 |