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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 52, 172 |
| AcoI | YGGCCR | 1 cut(s) | 68 |
| AflII | CTTAAG | 1 cut(s) | 97 |
| AjiI | CACGTC | 1 cut(s) | 167 |
| AjnI | CCWGG | 1 cut(s) | 153 |
| AoxI | GGCC | 2 cut(s) | 53, 68 |
| ApeKI | GCWGC | 1 cut(s) | 49 |
| AspLEI | GCGC | 4 cut(s) | 108, 132, 153, 177 |
| AspS9I | GGNCC | 1 cut(s) | 54 |
| BalI | TGGCCA | 1 cut(s) | 70 |
| BbvI | GCAGC | 1 cut(s) | 61 |
| BccI | CCATC | 1 cut(s) | 72 |
| BciT130I | CCWGG | 1 cut(s) | 155 |
| BfaI | CTAG | 1 cut(s) | 178 |
| BfoI | RGCGCY | 3 cut(s) | 109, 154, 178 |
| BfrI | CTTAAG | 1 cut(s) | 97 |
| BisI | GCNGC | 3 cut(s) | 50, 53, 173 |
| BlsI | GCNGC | 3 cut(s) | 51, 54, 174 |
| Bme1390I | CCNGG | 1 cut(s) | 155 |
| BmgBI | CACGTC | 1 cut(s) | 167 |
| BmgT120I | GGNCC | 1 cut(s) | 54 |
| BmrFI | CCNGG | 1 cut(s) | 155 |
| BpmI | CTGGAG | 1 cut(s) | 176 |
| BseBI | CCWGG | 1 cut(s) | 155 |
| BseXI | GCAGC | 1 cut(s) | 61 |
| BseYI | CCCAGC | 1 cut(s) | 126 |
| BshFI | GGCC | 2 cut(s) | 55, 70 |
| BslFI | GGGAC | 1 cut(s) | 151 |
| BsmFI | GGGAC | 1 cut(s) | 151 |
| BsnI | GGCC | 2 cut(s) | 55, 70 |
| Bsp143I | GATC | 1 cut(s) | 60 |
| BspACI | CCGC | 2 cut(s) | 52, 172 |
| BspANI | GGCC | 2 cut(s) | 55, 70 |
| BspTI | CTTAAG | 1 cut(s) | 97 |
| BssMI | GATC | 1 cut(s) | 60 |
| Bst2UI | CCWGG | 1 cut(s) | 155 |
| Bst6I | CTCTTC | 1 cut(s) | 33 |
| BstAFI | CTTAAG | 1 cut(s) | 97 |
| BstH2I | RGCGCY | 3 cut(s) | 109, 154, 178 |
| BstHHI | GCGC | 4 cut(s) | 108, 132, 153, 177 |
| BstKTI | GATC | 1 cut(s) | 63 |
| BstMBI | GATC | 1 cut(s) | 60 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 127, 157 |
| BstNI | CCWGG | 1 cut(s) | 155 |
| BstSCI | CCNGG | 1 cut(s) | 153 |
| BstV1I | GCAGC | 1 cut(s) | 61 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 78 |
| BsuRI | GGCC | 2 cut(s) | 55, 70 |
| BtrI | CACGTC | 1 cut(s) | 167 |
| BtsIMutI | CAGTG | 1 cut(s) | 73 |
| CfoI | GCGC | 4 cut(s) | 108, 132, 153, 177 |
| Cfr13I | GGNCC | 1 cut(s) | 54 |
| CviAII | CATG | 1 cut(s) | 18 |
| CviJI | RGCY | 2 cut(s) | 55, 70 |
| CviKI_1 | RGCY | 2 cut(s) | 55, 70 |
| DpnI | GATC | 1 cut(s) | 62 |
| DpnII | GATC | 1 cut(s) | 60 |
| EaeI | YGGCCR | 1 cut(s) | 68 |
| Eam1104I | CTCTTC | 1 cut(s) | 33 |
| EarI | CTCTTC | 1 cut(s) | 33 |
| EcoRII | CCWGG | 1 cut(s) | 153 |
| FaeI | CATG | 1 cut(s) | 21 |
| FaiI | YATR | 1 cut(s) | 19 |
| FaqI | GGGAC | 1 cut(s) | 151 |
| FatI | CATG | 1 cut(s) | 17 |
| Fnu4HI | GCNGC | 3 cut(s) | 50, 53, 173 |
| Fsp4HI | GCNGC | 3 cut(s) | 50, 53, 173 |
| FspBI | CTAG | 1 cut(s) | 178 |
| GlaI | GCGC | 4 cut(s) | 107, 131, 152, 176 |
| GluI | GCNGC | 3 cut(s) | 50, 53, 173 |
| GsaI | CCCAGC | 1 cut(s) | 130 |
| GsuI | CTGGAG | 1 cut(s) | 176 |
| HaeII | RGCGCY | 3 cut(s) | 109, 154, 178 |
| HaeIII | GGCC | 2 cut(s) | 55, 70 |
| HhaI | GCGC | 4 cut(s) | 108, 132, 153, 177 |
| Hin1II | CATG | 1 cut(s) | 21 |
| Hin6I | GCGC | 4 cut(s) | 106, 130, 151, 175 |
| HinP1I | GCGC | 4 cut(s) | 106, 130, 151, 175 |
| Hpy166II | GTNNAC | 1 cut(s) | 15 |
| Hpy8I | GTNNAC | 1 cut(s) | 15 |
| HpyCH4IV | ACGT | 1 cut(s) | 166 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 127, 157 |
| HpySE526I | ACGT | 1 cut(s) | 166 |
| Hsp92II | CATG | 1 cut(s) | 21 |
| HspAI | GCGC | 4 cut(s) | 106, 130, 151, 175 |
| Kzo9I | GATC | 1 cut(s) | 60 |
| LmnI | GCTCC | 1 cut(s) | 157 |
| LpnPI | CCDG | 3 cut(s) | 140, 140, 167 |
| Lsp1109I | GCAGC | 1 cut(s) | 61 |
| MaeI | CTAG | 1 cut(s) | 178 |
| MaeII | ACGT | 1 cut(s) | 166 |
| MaeIII | GTNAC | 1 cut(s) | 162 |
| MalI | GATC | 1 cut(s) | 62 |
| MboI | GATC | 1 cut(s) | 60 |
| MboII | GAAGA | 1 cut(s) | 50 |
| MlsI | TGGCCA | 1 cut(s) | 70 |
| MluNI | TGGCCA | 1 cut(s) | 70 |
| MnlI | CCTC | 1 cut(s) | 95 |
| Mox20I | TGGCCA | 1 cut(s) | 70 |
| MscI | TGGCCA | 1 cut(s) | 70 |
| MseI | TTAA | 1 cut(s) | 98 |
| MslI | CAYNNNNRTG | 1 cut(s) | 76 |
| Msp20I | TGGCCA | 1 cut(s) | 70 |
| MspA1I | CMGCKG | 2 cut(s) | 52, 172 |
| MspCI | CTTAAG | 1 cut(s) | 97 |
| MspR9I | CCNGG | 1 cut(s) | 155 |
| MvaI | CCWGG | 1 cut(s) | 155 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 127, 157 |
| NdeII | GATC | 1 cut(s) | 60 |
| NlaIII | CATG | 1 cut(s) | 21 |
| NmuCI | GTSAC | 1 cut(s) | 162 |
| PkrI | GCNGC | 3 cut(s) | 51, 54, 174 |
| Psp6I | CCWGG | 1 cut(s) | 153 |
| PspFI | CCCAGC | 1 cut(s) | 126 |
| PspGI | CCWGG | 1 cut(s) | 153 |
| PspPI | GGNCC | 1 cut(s) | 54 |
| RseI | CAYNNNNRTG | 1 cut(s) | 76 |
| SaqAI | TTAA | 1 cut(s) | 98 |
| SatI | GCNGC | 3 cut(s) | 50, 53, 173 |
| Sau3AI | GATC | 1 cut(s) | 60 |
| Sau96I | GGNCC | 1 cut(s) | 54 |
| ScrFI | CCNGG | 1 cut(s) | 155 |
| SetI | ASST | 2 cut(s) | 14, 169 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 76 |
| SmlI | CTYRAG | 1 cut(s) | 97 |
| SmoI | CTYRAG | 1 cut(s) | 97 |
| SsiI | CCGC | 2 cut(s) | 52, 172 |
| SspMI | CTAG | 1 cut(s) | 178 |
| StyD4I | CCNGG | 1 cut(s) | 153 |
| TaiI | ACGT | 1 cut(s) | 169 |
| TaqI | TCGA | 1 cut(s) | 89 |
| TauI | GCSGC | 2 cut(s) | 55, 175 |
| Tru1I | TTAA | 1 cut(s) | 98 |
| Tru9I | TTAA | 1 cut(s) | 98 |
| TscAI | CASTG | 1 cut(s) | 80 |
| TseFI | GTSAC | 1 cut(s) | 162 |
| TseI | GCWGC | 1 cut(s) | 49 |
| Tsp45I | GTSAC | 1 cut(s) | 162 |
| TspDTI | ATGAA | 1 cut(s) | 107 |
| TspRI | CASTG | 1 cut(s) | 80 |
| Vha464I | CTTAAG | 1 cut(s) | 97 |
| XspI | CTAG | 1 cut(s) | 178 |