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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 397 |
| Acc36I | ACCTGC | 2 cut(s) | 397, 544 |
| Acc65I | GGTACC | 3 cut(s) | 250, 529, 556 |
| AccB1I | GGYRCC | 3 cut(s) | 250, 529, 556 |
| AccBSI | CCGCTC | 1 cut(s) | 288 |
| AciI | CCGC | 9 cut(s) | 38, 102, 193, 225, 254, 272, 288, 318, 490 |
| AclWI | GGATC | 2 cut(s) | 29, 318 |
| AcoI | YGGCCR | 2 cut(s) | 281, 411 |
| AcsI | RAATTY | 1 cut(s) | 428 |
| AcuI | CTGAAG | 1 cut(s) | 461 |
| AfaI | GTAC | 4 cut(s) | 252, 306, 531, 558 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 44, 226, 301, 307, 352, 406 |
| AgsI | TTSAA | 1 cut(s) | 565 |
| AjnI | CCWGG | 2 cut(s) | 234, 246 |
| AluBI | AGCT | 5 cut(s) | 11, 331, 367, 439, 577 |
| AluI | AGCT | 5 cut(s) | 11, 331, 367, 439, 577 |
| Alw21I | GWGCWC | 3 cut(s) | 13, 369, 497 |
| Alw26I | GTCTC | 1 cut(s) | 389 |
| AlwI | GGATC | 2 cut(s) | 29, 318 |
| Ama87I | CYCGRG | 1 cut(s) | 368 |
| AoxI | GGCC | 3 cut(s) | 281, 390, 411 |
| ApeKI | GCWGC | 1 cut(s) | 291 |
| ApoI | RAATTY | 1 cut(s) | 428 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 131 |
| Asp718I | GGTACC | 3 cut(s) | 250, 529, 556 |
| AspLEI | GCGC | 3 cut(s) | 451, 475, 504 |
| AspS9I | GGNCC | 4 cut(s) | 47, 343, 536, 590 |
| AsuC2I | CCSGG | 2 cut(s) | 302, 534 |
| AvaI | CYCGRG | 1 cut(s) | 368 |
| AvaII | GGWCC | 4 cut(s) | 47, 343, 536, 590 |
| BalI | TGGCCA | 1 cut(s) | 413 |
| BanI | GGYRCC | 3 cut(s) | 250, 529, 556 |
| BanII | GRGCYC | 2 cut(s) | 13, 369 |
| Bbv12I | GWGCWC | 3 cut(s) | 13, 369, 497 |
| BbvCI | CCTCAGC | 1 cut(s) | 114 |
| BbvI | GCAGC | 1 cut(s) | 303 |
| BccI | CCATC | 2 cut(s) | 50, 64 |
| BciT130I | CCWGG | 2 cut(s) | 236, 248 |
| BclI | TGATCA | 1 cut(s) | 162 |
| BcnI | CCSGG | 2 cut(s) | 302, 534 |
| BcoDI | GTCTC | 1 cut(s) | 389 |
| BfaI | CTAG | 3 cut(s) | 240, 498, 572 |
| BfmI | CTRYAG | 1 cut(s) | 267 |
| BfoI | RGCGCY | 1 cut(s) | 452 |
| BfuAI | ACCTGC | 2 cut(s) | 397, 544 |
| BisI | GCNGC | 3 cut(s) | 289, 292, 318 |
| BlsI | GCNGC | 3 cut(s) | 290, 293, 319 |
| Bme1390I | CCNGG | 4 cut(s) | 236, 248, 302, 534 |
| Bme18I | GGWCC | 4 cut(s) | 47, 343, 536, 590 |
| BmeT110I | CYCGRG | 1 cut(s) | 368 |
| BmgT120I | GGNCC | 4 cut(s) | 47, 343, 536, 590 |
| BmiI | GGNNCC | 5 cut(s) | 231, 252, 531, 558, 591 |
| BmrFI | CCNGG | 4 cut(s) | 236, 248, 302, 534 |
| BmsI | GCATC | 1 cut(s) | 540 |
| Bpu10I | CCTNAGC | 2 cut(s) | 114, 600 |
| BpuMI | CCSGG | 2 cut(s) | 302, 534 |
| BsaBI | GATNNNNATC | 1 cut(s) | 33 |
| BsaI | GGTCTC | 1 cut(s) | 389 |
| BsaJI | CCNNGG | 3 cut(s) | 234, 246, 300 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 44, 226, 301, 307, 352, 406 |
| Bse118I | RCCGGY | 1 cut(s) | 211 |
| Bse1I | ACTGG | 1 cut(s) | 342 |
| Bse8I | GATNNNNATC | 1 cut(s) | 33 |
| BseBI | CCWGG | 2 cut(s) | 236, 248 |
| BseDI | CCNNGG | 3 cut(s) | 234, 246, 300 |
| BseGI | GGATG | 3 cut(s) | 56, 66, 80 |
| BseJI | GATNNNNATC | 1 cut(s) | 33 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 44, 226, 301, 307, 352, 406 |
| BseMII | CTCAG | 2 cut(s) | 105, 591 |
| BseNI | ACTGG | 1 cut(s) | 342 |
| BseRI | GAGGAG | 2 cut(s) | 131, 597 |
| BseX3I | CGGCCG | 1 cut(s) | 281 |
| BseXI | GCAGC | 1 cut(s) | 303 |
| BseYI | CCCAGC | 2 cut(s) | 221, 469 |
| BsgI | GTGCAG | 1 cut(s) | 400 |
| Bsh1285I | CGRYCG | 1 cut(s) | 284 |
| BshFI | GGCC | 3 cut(s) | 283, 392, 413 |
| BshNI | GGYRCC | 3 cut(s) | 250, 529, 556 |
| BsiEI | CGRYCG | 1 cut(s) | 284 |
| BsiHKAI | GWGCWC | 3 cut(s) | 13, 369, 497 |
| BsiHKCI | CYCGRG | 1 cut(s) | 368 |
| BsiSI | CCGG | 3 cut(s) | 212, 302, 534 |
| BslFI | GGGAC | 1 cut(s) | 603 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 44, 226, 301, 307, 352, 406 |
| BsmAI | GTCTC | 1 cut(s) | 389 |
| BsmFI | GGGAC | 1 cut(s) | 603 |
| BsnI | GGCC | 3 cut(s) | 283, 392, 413 |
| Bso31I | GGTCTC | 1 cut(s) | 389 |
| BsoBI | CYCGRG | 1 cut(s) | 368 |
| Bsp1286I | GDGCHC | 3 cut(s) | 13, 369, 497 |
| Bsp143I | GATC | 3 cut(s) | 34, 162, 310 |
| BspACI | CCGC | 9 cut(s) | 38, 102, 193, 225, 254, 272, 288, 318, 490 |
| BspANI | GGCC | 3 cut(s) | 283, 392, 413 |
| BspCNI | CTCAG | 2 cut(s) | 106, 592 |
| BspLI | GGNNCC | 5 cut(s) | 231, 252, 531, 558, 591 |
| BspMI | ACCTGC | 2 cut(s) | 397, 544 |
| BspPI | GGATC | 2 cut(s) | 29, 318 |
| BspT107I | GGYRCC | 3 cut(s) | 250, 529, 556 |
| BspTNI | GGTCTC | 1 cut(s) | 389 |
| BsrBI | CCGCTC | 1 cut(s) | 288 |
| BsrFI | RCCGGY | 1 cut(s) | 211 |
| BsrI | ACTGG | 1 cut(s) | 342 |
| BssAI | RCCGGY | 1 cut(s) | 211 |
| BssECI | CCNNGG | 3 cut(s) | 234, 246, 300 |
| BssMI | GATC | 3 cut(s) | 34, 162, 310 |
| Bst2UI | CCWGG | 2 cut(s) | 236, 248 |
| Bst4CI | ACNGT | 2 cut(s) | 98, 561 |
| BstC8I | GCNNGC | 4 cut(s) | 123, 209, 333, 482 |
| BstDEI | CTNAG | 2 cut(s) | 114, 600 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 350 |
| BstF5I | GGATG | 3 cut(s) | 56, 66, 80 |
| BstH2I | RGCGCY | 1 cut(s) | 452 |
| BstHHI | GCGC | 3 cut(s) | 451, 475, 504 |
| BstKTI | GATC | 3 cut(s) | 37, 165, 313 |
| BstMAI | GTCTC | 1 cut(s) | 389 |
| BstMBI | GATC | 3 cut(s) | 34, 162, 310 |
| BstMCI | CGRYCG | 1 cut(s) | 284 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 455, 470, 481, 501, 508 |
| BstNI | CCWGG | 2 cut(s) | 236, 248 |
| BstSCI | CCNGG | 4 cut(s) | 234, 246, 300, 532 |
| BstSFI | CTRYAG | 1 cut(s) | 267 |
| BstV1I | GCAGC | 1 cut(s) | 303 |
| BstX2I | RGATCY | 1 cut(s) | 310 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 526 |
| BstYI | RGATCY | 1 cut(s) | 310 |
| BstZI | CGGCCG | 1 cut(s) | 281 |
| BsuRI | GGCC | 3 cut(s) | 283, 392, 413 |
| BtsCI | GGATG | 3 cut(s) | 56, 66, 80 |
| BtsI | GCAGTG | 1 cut(s) | 491 |
| BtsIMutI | CAGTG | 2 cut(s) | 335, 491 |
| BveI | ACCTGC | 2 cut(s) | 397, 544 |
| Cac8I | GCNNGC | 4 cut(s) | 123, 209, 333, 482 |
| CfoI | GCGC | 3 cut(s) | 451, 475, 504 |
| Cfr10I | RCCGGY | 1 cut(s) | 211 |
| Cfr13I | GGNCC | 4 cut(s) | 47, 343, 536, 590 |
| Csp6I | GTAC | 4 cut(s) | 251, 305, 530, 557 |
| CviAII | CATG | 2 cut(s) | 146, 415 |
| CviQI | GTAC | 4 cut(s) | 251, 305, 530, 557 |
| DdeI | CTNAG | 2 cut(s) | 114, 600 |
| DpnI | GATC | 3 cut(s) | 36, 164, 312 |
| DpnII | GATC | 3 cut(s) | 34, 162, 310 |
| EaeI | YGGCCR | 2 cut(s) | 281, 411 |
| EagI | CGGCCG | 1 cut(s) | 281 |
| EciI | GGCGGA | 2 cut(s) | 27, 505 |
| Ecl136II | GAGCTC | 2 cut(s) | 11, 367 |
| EclXI | CGGCCG | 1 cut(s) | 281 |
| Eco24I | GRGCYC | 2 cut(s) | 13, 369 |
| Eco31I | GGTCTC | 1 cut(s) | 389 |
| Eco47I | GGWCC | 4 cut(s) | 47, 343, 536, 590 |
| Eco52I | CGGCCG | 1 cut(s) | 281 |
| Eco53kI | GAGCTC | 2 cut(s) | 11, 367 |
| Eco57I | CTGAAG | 1 cut(s) | 461 |
| Eco88I | CYCGRG | 1 cut(s) | 368 |
| EcoICRI | GAGCTC | 2 cut(s) | 11, 367 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 350 |
| EcoRII | CCWGG | 2 cut(s) | 234, 246 |
| EcoT38I | GRGCYC | 2 cut(s) | 13, 369 |
| FaeI | CATG | 2 cut(s) | 149, 418 |
| FaiI | YATR | 5 cut(s) | 147, 323, 327, 416, 545 |
| FaqI | GGGAC | 1 cut(s) | 603 |
| FatI | CATG | 2 cut(s) | 145, 414 |
| FauI | CCCGC | 2 cut(s) | 95, 218 |
| FbaI | TGATCA | 1 cut(s) | 162 |
| Fnu4HI | GCNGC | 3 cut(s) | 289, 292, 318 |
| FokI | GGATG | 3 cut(s) | 43, 53, 67 |
| FriOI | GRGCYC | 2 cut(s) | 13, 369 |
| Fsp4HI | GCNGC | 3 cut(s) | 289, 292, 318 |
| FspBI | CTAG | 3 cut(s) | 240, 498, 572 |
| GlaI | GCGC | 3 cut(s) | 450, 474, 503 |
| GluI | GCNGC | 3 cut(s) | 289, 292, 318 |
| GsaI | CCCAGC | 2 cut(s) | 225, 473 |
| HaeII | RGCGCY | 1 cut(s) | 452 |
| HaeIII | GGCC | 3 cut(s) | 283, 392, 413 |
| HapII | CCGG | 3 cut(s) | 212, 302, 534 |
| HhaI | GCGC | 3 cut(s) | 451, 475, 504 |
| Hin1II | CATG | 2 cut(s) | 149, 418 |
| Hin6I | GCGC | 3 cut(s) | 449, 473, 502 |
| HinP1I | GCGC | 3 cut(s) | 449, 473, 502 |
| HincII | GTYRAC | 2 cut(s) | 190, 217 |
| HindII | GTYRAC | 2 cut(s) | 190, 217 |
| HinfI | GANTC | 1 cut(s) | 127 |
| HpaI | GTTAAC | 1 cut(s) | 190 |
| HpaII | CCGG | 3 cut(s) | 212, 302, 534 |
| Hpy166II | GTNNAC | 3 cut(s) | 190, 217, 343 |
| Hpy188I | TCNGA | 1 cut(s) | 47 |
| Hpy188III | TCNNGA | 1 cut(s) | 599 |
| Hpy8I | GTNNAC | 3 cut(s) | 190, 217, 343 |
| HpyAV | CCTTC | 2 cut(s) | 356, 439 |
| HpyCH4III | ACNGT | 2 cut(s) | 98, 561 |
| HpyCH4IV | ACGT | 1 cut(s) | 168 |
| HpyCH4V | TGCA | 2 cut(s) | 381, 553 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 455, 470, 481, 501, 508 |
| HpyF3I | CTNAG | 2 cut(s) | 114, 600 |
| HpySE526I | ACGT | 1 cut(s) | 168 |
| Hsp92II | CATG | 2 cut(s) | 149, 418 |
| HspAI | GCGC | 3 cut(s) | 449, 473, 502 |
| KpnI | GGTACC | 3 cut(s) | 254, 533, 560 |
| Ksp22I | TGATCA | 1 cut(s) | 162 |
| KspAI | GTTAAC | 1 cut(s) | 190 |
| Kzo9I | GATC | 3 cut(s) | 34, 162, 310 |
| LmnI | GCTCC | 3 cut(s) | 8, 118, 492 |
| Lsp1109I | GCAGC | 1 cut(s) | 303 |
| LweI | GCATC | 1 cut(s) | 540 |
| MaeI | CTAG | 3 cut(s) | 240, 498, 572 |
| MaeII | ACGT | 1 cut(s) | 168 |
| MalI | GATC | 3 cut(s) | 36, 164, 312 |
| MbiI | CCGCTC | 1 cut(s) | 288 |
| MboI | GATC | 3 cut(s) | 34, 162, 310 |
| MboII | GAAGA | 1 cut(s) | 454 |
| MflI | RGATCY | 1 cut(s) | 310 |
| MhlI | GDGCHC | 3 cut(s) | 13, 369, 497 |
| MlsI | TGGCCA | 1 cut(s) | 413 |
| MluCI | AATT | 2 cut(s) | 131, 428 |
| MluNI | TGGCCA | 1 cut(s) | 413 |
| MnlI | CCTC | 5 cut(s) | 109, 268, 575, 606, 615 |
| Mox20I | TGGCCA | 1 cut(s) | 413 |
| MroXI | GAANNNNTTC | 1 cut(s) | 131 |
| MscI | TGGCCA | 1 cut(s) | 413 |
| MseI | TTAA | 3 cut(s) | 183, 189, 425 |
| MslI | CAYNNNNRTG | 2 cut(s) | 419, 548 |
| Msp20I | TGGCCA | 1 cut(s) | 413 |
| MspA1I | CMGCKG | 2 cut(s) | 225, 272 |
| MspI | CCGG | 3 cut(s) | 212, 302, 534 |
| MspR9I | CCNGG | 4 cut(s) | 236, 248, 302, 534 |
| MvaI | CCWGG | 2 cut(s) | 236, 248 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 455, 470, 481, 501, 508 |
| NciI | CCSGG | 2 cut(s) | 302, 534 |
| NdeII | GATC | 3 cut(s) | 34, 162, 310 |
| NlaIII | CATG | 2 cut(s) | 149, 418 |
| NlaIV | GGNNCC | 5 cut(s) | 231, 252, 531, 558, 591 |
| NmeAIII | GCCGAG | 3 cut(s) | 259, 309, 418 |
| PaeR7I | CTCGAG | 1 cut(s) | 368 |
| PaqCI | CACCTGC | 1 cut(s) | 397 |
| PdmI | GAANNNNTTC | 1 cut(s) | 131 |
| PfeI | GAWTC | 1 cut(s) | 127 |
| PkrI | GCNGC | 3 cut(s) | 290, 293, 319 |
| Psp124BI | GAGCTC | 2 cut(s) | 13, 369 |
| Psp6I | CCWGG | 2 cut(s) | 234, 246 |
| PspFI | CCCAGC | 2 cut(s) | 221, 469 |
| PspGI | CCWGG | 2 cut(s) | 234, 246 |
| PspN4I | GGNNCC | 5 cut(s) | 231, 252, 531, 558, 591 |
| PspPI | GGNCC | 4 cut(s) | 47, 343, 536, 590 |
| PspXI | VCTCGAGB | 1 cut(s) | 368 |
| PsuI | RGATCY | 1 cut(s) | 310 |
| RsaI | GTAC | 4 cut(s) | 252, 306, 531, 558 |
| RsaNI | GTAC | 4 cut(s) | 251, 305, 530, 557 |
| RseI | CAYNNNNRTG | 2 cut(s) | 419, 548 |
| SacI | GAGCTC | 2 cut(s) | 13, 369 |
| SaqAI | TTAA | 3 cut(s) | 183, 189, 425 |
| SatI | GCNGC | 3 cut(s) | 289, 292, 318 |
| Sau3AI | GATC | 3 cut(s) | 34, 162, 310 |
| Sau96I | GGNCC | 4 cut(s) | 47, 343, 536, 590 |
| ScrFI | CCNGG | 4 cut(s) | 236, 248, 302, 534 |
| SduI | GDGCHC | 3 cut(s) | 13, 369, 497 |
| SfaNI | GCATC | 1 cut(s) | 540 |
| SfcI | CTRYAG | 1 cut(s) | 267 |
| Sfr274I | CTCGAG | 1 cut(s) | 368 |
| SinI | GGWCC | 4 cut(s) | 47, 343, 536, 590 |
| SlaI | CTCGAG | 1 cut(s) | 368 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 419, 548 |
| SmlI | CTYRAG | 1 cut(s) | 368 |
| SmoI | CTYRAG | 1 cut(s) | 368 |
| Sse9I | AATT | 2 cut(s) | 131, 428 |
| SsiI | CCGC | 9 cut(s) | 38, 102, 193, 225, 254, 272, 288, 318, 490 |
| SspMI | CTAG | 3 cut(s) | 240, 498, 572 |
| SstI | GAGCTC | 2 cut(s) | 13, 369 |
| StyD4I | CCNGG | 4 cut(s) | 234, 246, 300, 532 |
| TaaI | ACNGT | 2 cut(s) | 98, 561 |
| TaiI | ACGT | 1 cut(s) | 171 |
| TaqI | TCGA | 2 cut(s) | 369, 607 |
| TasI | AATT | 2 cut(s) | 131, 428 |
| TauI | GCSGC | 2 cut(s) | 291, 320 |
| TfiI | GAWTC | 1 cut(s) | 127 |
| Tru1I | TTAA | 3 cut(s) | 183, 189, 425 |
| Tru9I | TTAA | 3 cut(s) | 183, 189, 425 |
| TscAI | CASTG | 2 cut(s) | 342, 491 |
| TseI | GCWGC | 1 cut(s) | 291 |
| TspGWI | ACGGA | 1 cut(s) | 537 |
| TspRI | CASTG | 2 cut(s) | 342, 491 |
| VpaK11BI | GGWCC | 4 cut(s) | 47, 343, 536, 590 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 350 |
| XapI | RAATTY | 1 cut(s) | 428 |
| XhoI | CTCGAG | 1 cut(s) | 368 |
| XmnI | GAANNNNTTC | 1 cut(s) | 131 |
| XspI | CTAG | 3 cut(s) | 240, 498, 572 |