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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 295 |
| Acc65I | GGTACC | 1 cut(s) | 307 |
| AccB1I | GGYRCC | 2 cut(s) | 199, 307 |
| AccBSI | CCGCTC | 1 cut(s) | 405 |
| AccII | CGCG | 1 cut(s) | 241 |
| AciI | CCGC | 6 cut(s) | 23, 69, 241, 392, 403, 410 |
| AclI | AACGTT | 1 cut(s) | 213 |
| AclWI | GGATC | 1 cut(s) | 69 |
| AcsI | RAATTY | 1 cut(s) | 179 |
| AcyI | GRCGYC | 1 cut(s) | 200 |
| AfaI | GTAC | 3 cut(s) | 282, 288, 309 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 157, 269, 367, 409 |
| AjnI | CCWGG | 3 cut(s) | 52, 57, 419 |
| AluBI | AGCT | 2 cut(s) | 82, 328 |
| AluI | AGCT | 2 cut(s) | 82, 328 |
| Alw21I | GWGCWC | 1 cut(s) | 248 |
| AlwI | GGATC | 1 cut(s) | 69 |
| AoxI | GGCC | 4 cut(s) | 55, 147, 162, 417 |
| ApeKI | GCWGC | 2 cut(s) | 20, 39 |
| ApoI | RAATTY | 1 cut(s) | 179 |
| Asp718I | GGTACC | 1 cut(s) | 307 |
| AspLEI | GCGC | 3 cut(s) | 132, 202, 226 |
| AspS9I | GGNCC | 3 cut(s) | 148, 340, 417 |
| AsuNHI | GCTAGC | 1 cut(s) | 82 |
| AvaII | GGWCC | 1 cut(s) | 340 |
| BanI | GGYRCC | 2 cut(s) | 199, 307 |
| Bbv12I | GWGCWC | 1 cut(s) | 248 |
| BbvI | GCAGC | 2 cut(s) | 32, 51 |
| BccI | CCATC | 2 cut(s) | 361, 365 |
| BciT130I | CCWGG | 3 cut(s) | 54, 59, 421 |
| BfaI | CTAG | 4 cut(s) | 35, 83, 323, 414 |
| BfmI | CTRYAG | 1 cut(s) | 18 |
| BfoI | RGCGCY | 1 cut(s) | 203 |
| BfuAI | ACCTGC | 1 cut(s) | 295 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 54 |
| BisI | GCNGC | 4 cut(s) | 21, 40, 69, 411 |
| BlsI | GCNGC | 4 cut(s) | 22, 41, 70, 412 |
| BmcAI | AGTACT | 1 cut(s) | 288 |
| Bme1390I | CCNGG | 3 cut(s) | 54, 59, 421 |
| Bme18I | GGWCC | 1 cut(s) | 340 |
| BmgT120I | GGNCC | 3 cut(s) | 148, 340, 417 |
| BmiI | GGNNCC | 2 cut(s) | 201, 309 |
| BmrFI | CCNGG | 3 cut(s) | 54, 59, 421 |
| BmsI | GCATC | 1 cut(s) | 291 |
| BmtI | GCTAGC | 1 cut(s) | 86 |
| BsaHI | GRCGYC | 1 cut(s) | 200 |
| BsaJI | CCNNGG | 1 cut(s) | 144 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 157, 269, 367, 409 |
| Bse1I | ACTGG | 2 cut(s) | 274, 284 |
| BseBI | CCWGG | 3 cut(s) | 54, 59, 421 |
| BseDI | CCNNGG | 1 cut(s) | 144 |
| BseGI | GGATG | 1 cut(s) | 357 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 157, 269, 367, 409 |
| BseNI | ACTGG | 2 cut(s) | 274, 284 |
| BseXI | GCAGC | 2 cut(s) | 32, 51 |
| BseYI | CCCAGC | 1 cut(s) | 220 |
| Bsh1236I | CGCG | 1 cut(s) | 241 |
| BshFI | GGCC | 4 cut(s) | 57, 149, 164, 419 |
| BshNI | GGYRCC | 2 cut(s) | 199, 307 |
| BsiHKAI | GWGCWC | 1 cut(s) | 248 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 157, 269, 367, 409 |
| BsnI | GGCC | 4 cut(s) | 57, 149, 164, 419 |
| Bsp1286I | GDGCHC | 1 cut(s) | 248 |
| Bsp143I | GATC | 1 cut(s) | 61 |
| BspACI | CCGC | 6 cut(s) | 23, 69, 241, 392, 403, 410 |
| BspANI | GGCC | 4 cut(s) | 57, 149, 164, 419 |
| BspFNI | CGCG | 1 cut(s) | 241 |
| BspLI | GGNNCC | 2 cut(s) | 201, 309 |
| BspMAI | CTGCAG | 1 cut(s) | 22 |
| BspMI | ACCTGC | 1 cut(s) | 295 |
| BspOI | GCTAGC | 1 cut(s) | 86 |
| BspPI | GGATC | 1 cut(s) | 69 |
| BspT107I | GGYRCC | 2 cut(s) | 199, 307 |
| BsrBI | CCGCTC | 1 cut(s) | 405 |
| BsrI | ACTGG | 2 cut(s) | 274, 284 |
| BssECI | CCNNGG | 1 cut(s) | 144 |
| BssMI | GATC | 1 cut(s) | 61 |
| BssNI | GRCGYC | 1 cut(s) | 200 |
| Bst2UI | CCWGG | 3 cut(s) | 54, 59, 421 |
| Bst4CI | ACNGT | 1 cut(s) | 312 |
| BstACI | GRCGYC | 1 cut(s) | 200 |
| BstC8I | GCNNGC | 1 cut(s) | 84 |
| BstF5I | GGATG | 1 cut(s) | 357 |
| BstFNI | CGCG | 1 cut(s) | 241 |
| BstH2I | RGCGCY | 1 cut(s) | 203 |
| BstHHI | GCGC | 3 cut(s) | 132, 202, 226 |
| BstKTI | GATC | 1 cut(s) | 64 |
| BstMBI | GATC | 1 cut(s) | 61 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 26, 45, 54, 206, 232, 416 |
| BstNI | CCWGG | 3 cut(s) | 54, 59, 421 |
| BstSCI | CCNGG | 3 cut(s) | 52, 57, 419 |
| BstSFI | CTRYAG | 1 cut(s) | 18 |
| BstUI | CGCG | 1 cut(s) | 241 |
| BstV1I | GCAGC | 2 cut(s) | 32, 51 |
| BstX2I | RGATCY | 1 cut(s) | 61 |
| BstYI | RGATCY | 1 cut(s) | 61 |
| BsuRI | GGCC | 4 cut(s) | 57, 149, 164, 419 |
| BtsCI | GGATG | 1 cut(s) | 357 |
| BveI | ACCTGC | 1 cut(s) | 295 |
| Cac8I | GCNNGC | 1 cut(s) | 84 |
| CfoI | GCGC | 3 cut(s) | 132, 202, 226 |
| Cfr13I | GGNCC | 3 cut(s) | 148, 340, 417 |
| Csp6I | GTAC | 3 cut(s) | 281, 287, 308 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 253, 288 |
| CviJI | RGCY | 8 cut(s) | 57, 82, 143, 149, 164, 328, 413, 419 |
| CviKI_1 | RGCY | 8 cut(s) | 57, 82, 143, 149, 164, 328, 413, 419 |
| CviQI | GTAC | 3 cut(s) | 281, 287, 308 |
| DinI | GGCGCC | 1 cut(s) | 201 |
| DpnI | GATC | 1 cut(s) | 63 |
| DpnII | GATC | 1 cut(s) | 61 |
| Eco47I | GGWCC | 1 cut(s) | 340 |
| EcoRII | CCWGG | 3 cut(s) | 52, 57, 419 |
| EgeI | GGCGCC | 1 cut(s) | 201 |
| EheI | GGCGCC | 1 cut(s) | 201 |
| FaiI | YATR | 5 cut(s) | 74, 78, 173, 255, 296 |
| Fnu4HI | GCNGC | 4 cut(s) | 21, 40, 69, 411 |
| FokI | GGATG | 1 cut(s) | 344 |
| Fsp4HI | GCNGC | 4 cut(s) | 21, 40, 69, 411 |
| FspBI | CTAG | 4 cut(s) | 35, 83, 323, 414 |
| GlaI | GCGC | 3 cut(s) | 131, 201, 225 |
| GluI | GCNGC | 4 cut(s) | 21, 40, 69, 411 |
| GsaI | CCCAGC | 1 cut(s) | 224 |
| HaeII | RGCGCY | 1 cut(s) | 203 |
| HaeIII | GGCC | 4 cut(s) | 57, 149, 164, 419 |
| HhaI | GCGC | 3 cut(s) | 132, 202, 226 |
| Hin1I | GRCGYC | 1 cut(s) | 200 |
| Hin6I | GCGC | 3 cut(s) | 130, 200, 224 |
| HinP1I | GCGC | 3 cut(s) | 130, 200, 224 |
| HincII | GTYRAC | 3 cut(s) | 9, 217, 260 |
| HindII | GTYRAC | 3 cut(s) | 9, 217, 260 |
| HinfI | GANTC | 2 cut(s) | 316, 383 |
| HpaI | GTTAAC | 2 cut(s) | 9, 217 |
| Hpy166II | GTNNAC | 4 cut(s) | 9, 122, 217, 260 |
| Hpy8I | GTNNAC | 4 cut(s) | 9, 122, 217, 260 |
| Hpy99I | CGWCG | 2 cut(s) | 188, 316 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4III | ACNGT | 1 cut(s) | 312 |
| HpyCH4IV | ACGT | 1 cut(s) | 213 |
| HpyCH4V | TGCA | 2 cut(s) | 20, 304 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 26, 45, 54, 206, 232, 416 |
| HpySE526I | ACGT | 1 cut(s) | 213 |
| Hsp92I | GRCGYC | 1 cut(s) | 200 |
| HspAI | GCGC | 3 cut(s) | 130, 200, 224 |
| KasI | GGCGCC | 1 cut(s) | 199 |
| KpnI | GGTACC | 1 cut(s) | 311 |
| KspAI | GTTAAC | 2 cut(s) | 9, 217 |
| Kzo9I | GATC | 1 cut(s) | 61 |
| LmnI | GCTCC | 2 cut(s) | 115, 243 |
| Lsp1109I | GCAGC | 2 cut(s) | 32, 51 |
| LweI | GCATC | 1 cut(s) | 291 |
| MaeI | CTAG | 4 cut(s) | 35, 83, 323, 414 |
| MaeII | ACGT | 1 cut(s) | 213 |
| MaeIII | GTNAC | 1 cut(s) | 384 |
| MalI | GATC | 1 cut(s) | 63 |
| MbiI | CCGCTC | 1 cut(s) | 405 |
| MboI | GATC | 1 cut(s) | 61 |
| MflI | RGATCY | 1 cut(s) | 61 |
| MhlI | GDGCHC | 1 cut(s) | 248 |
| MluCI | AATT | 1 cut(s) | 179 |
| Mly113I | GGCGCC | 1 cut(s) | 200 |
| MlyI | GAGTC | 1 cut(s) | 392 |
| MnlI | CCTC | 5 cut(s) | 19, 107, 139, 154, 357 |
| MseI | TTAA | 2 cut(s) | 8, 216 |
| MslI | CAYNNNNRTG | 3 cut(s) | 170, 299, 362 |
| MspA1I | CMGCKG | 1 cut(s) | 23 |
| MspR9I | CCNGG | 3 cut(s) | 54, 59, 421 |
| MvaI | CCWGG | 3 cut(s) | 54, 59, 421 |
| MvnI | CGCG | 1 cut(s) | 241 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 26, 45, 54, 206, 232, 416 |
| NarI | GGCGCC | 1 cut(s) | 200 |
| NdeII | GATC | 1 cut(s) | 61 |
| NheI | GCTAGC | 1 cut(s) | 82 |
| NlaIV | GGNNCC | 2 cut(s) | 201, 309 |
| NmeAIII | GCCGAG | 2 cut(s) | 169, 386 |
| NmuCI | GTSAC | 1 cut(s) | 384 |
| PfeI | GAWTC | 1 cut(s) | 316 |
| PflFI | GACNNNGTC | 1 cut(s) | 255 |
| PkrI | GCNGC | 4 cut(s) | 22, 41, 70, 412 |
| PleI | GAGTC | 1 cut(s) | 391 |
| PluTI | GGCGCC | 1 cut(s) | 203 |
| PpsI | GAGTC | 1 cut(s) | 391 |
| Psp1406I | AACGTT | 1 cut(s) | 213 |
| Psp6I | CCWGG | 3 cut(s) | 52, 57, 419 |
| PspFI | CCCAGC | 1 cut(s) | 220 |
| PspGI | CCWGG | 3 cut(s) | 52, 57, 419 |
| PspN4I | GGNNCC | 2 cut(s) | 201, 309 |
| PspPI | GGNCC | 3 cut(s) | 148, 340, 417 |
| PstI | CTGCAG | 1 cut(s) | 22 |
| PsuI | RGATCY | 1 cut(s) | 61 |
| PsyI | GACNNNGTC | 1 cut(s) | 255 |
| RsaI | GTAC | 3 cut(s) | 282, 288, 309 |
| RsaNI | GTAC | 3 cut(s) | 281, 287, 308 |
| RseI | CAYNNNNRTG | 3 cut(s) | 170, 299, 362 |
| SaqAI | TTAA | 2 cut(s) | 8, 216 |
| SatI | GCNGC | 4 cut(s) | 21, 40, 69, 411 |
| Sau3AI | GATC | 1 cut(s) | 61 |
| Sau96I | GGNCC | 3 cut(s) | 148, 340, 417 |
| ScaI | AGTACT | 1 cut(s) | 288 |
| SchI | GAGTC | 1 cut(s) | 392 |
| ScrFI | CCNGG | 3 cut(s) | 54, 59, 421 |
| SduI | GDGCHC | 1 cut(s) | 248 |
| SetI | ASST | 7 cut(s) | 84, 99, 216, 309, 330, 345, 349 |
| SfaNI | GCATC | 1 cut(s) | 291 |
| SfcI | CTRYAG | 1 cut(s) | 18 |
| SfoI | GGCGCC | 1 cut(s) | 201 |
| SinI | GGWCC | 1 cut(s) | 340 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 170, 299, 362 |
| Sse9I | AATT | 1 cut(s) | 179 |
| SsiI | CCGC | 6 cut(s) | 23, 69, 241, 392, 403, 410 |
| SspDI | GGCGCC | 1 cut(s) | 199 |
| SspMI | CTAG | 4 cut(s) | 35, 83, 323, 414 |
| StyD4I | CCNGG | 3 cut(s) | 52, 57, 419 |
| TaaI | ACNGT | 1 cut(s) | 312 |
| TaiI | ACGT | 1 cut(s) | 216 |
| TaqI | TCGA | 2 cut(s) | 183, 314 |
| TasI | AATT | 1 cut(s) | 179 |
| TatI | WGTACW | 1 cut(s) | 286 |
| TauI | GCSGC | 2 cut(s) | 71, 413 |
| TfiI | GAWTC | 1 cut(s) | 316 |
| Tru1I | TTAA | 2 cut(s) | 8, 216 |
| Tru9I | TTAA | 2 cut(s) | 8, 216 |
| TseFI | GTSAC | 1 cut(s) | 384 |
| TseI | GCWGC | 2 cut(s) | 20, 39 |
| Tsp45I | GTSAC | 1 cut(s) | 384 |
| Tth111I | GACNNNGTC | 1 cut(s) | 255 |
| VpaK11BI | GGWCC | 1 cut(s) | 340 |
| XapI | RAATTY | 1 cut(s) | 179 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 364 |
| XspI | CTAG | 4 cut(s) | 35, 83, 323, 414 |
| ZrmI | AGTACT | 1 cut(s) | 288 |