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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 366 |
| AccII | CGCG | 3 cut(s) | 91, 191, 312 |
| AcoI | YGGCCR | 2 cut(s) | 107, 233 |
| AcuI | CTGAAG | 1 cut(s) | 512 |
| AfaI | GTAC | 1 cut(s) | 359 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 48, 49, 90, 210, 340 |
| AjnI | CCWGG | 3 cut(s) | 128, 478, 539 |
| Alw21I | GWGCWC | 2 cut(s) | 21, 319 |
| Alw26I | GTCTC | 1 cut(s) | 91 |
| Ama87I | CYCGRG | 1 cut(s) | 56 |
| AoxI | GGCC | 6 cut(s) | 52, 107, 218, 233, 405, 547 |
| ApaI | GGGCCC | 3 cut(s) | 56, 409, 551 |
| ApeKI | GCWGC | 1 cut(s) | 475 |
| AspLEI | GCGC | 1 cut(s) | 191 |
| AspS9I | GGNCC | 9 cut(s) | 52, 53, 126, 132, 219, 405, 406, 547, 548 |
| AsuC2I | CCSGG | 2 cut(s) | 57, 58 |
| AvaI | CYCGRG | 1 cut(s) | 56 |
| AvaII | GGWCC | 2 cut(s) | 126, 132 |
| BaeGI | GKGCMC | 3 cut(s) | 56, 409, 551 |
| BalI | TGGCCA | 1 cut(s) | 235 |
| BanII | GRGCYC | 4 cut(s) | 56, 64, 409, 551 |
| Bbv12I | GWGCWC | 2 cut(s) | 21, 319 |
| BbvI | GCAGC | 1 cut(s) | 487 |
| BciT130I | CCWGG | 3 cut(s) | 130, 480, 541 |
| BcnI | CCSGG | 2 cut(s) | 57, 58 |
| BcoDI | GTCTC | 1 cut(s) | 91 |
| BfaI | CTAG | 2 cut(s) | 44, 274 |
| BfuAI | ACCTGC | 1 cut(s) | 366 |
| BisI | GCNGC | 3 cut(s) | 92, 140, 476 |
| BlsI | GCNGC | 3 cut(s) | 93, 141, 477 |
| Bme1390I | CCNGG | 5 cut(s) | 57, 58, 130, 480, 541 |
| Bme18I | GGWCC | 2 cut(s) | 126, 132 |
| BmeT110I | CYCGRG | 1 cut(s) | 56 |
| BmgT120I | GGNCC | 9 cut(s) | 52, 53, 126, 132, 219, 405, 406, 547, 548 |
| BmiI | GGNNCC | 5 cut(s) | 53, 54, 55, 407, 549 |
| BmrFI | CCNGG | 5 cut(s) | 57, 58, 130, 480, 541 |
| BmsI | GCATC | 3 cut(s) | 166, 313, 362 |
| BpmI | CTGGAG | 1 cut(s) | 17 |
| BpuMI | CCSGG | 2 cut(s) | 57, 58 |
| BsaJI | CCNNGG | 5 cut(s) | 55, 56, 57, 479, 551 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 48, 49, 90, 210, 340 |
| Bse118I | RCCGGY | 1 cut(s) | 105 |
| Bse1I | ACTGG | 1 cut(s) | 34 |
| BseBI | CCWGG | 3 cut(s) | 130, 480, 541 |
| BseDI | CCNNGG | 5 cut(s) | 55, 56, 57, 479, 551 |
| BseGI | GGATG | 1 cut(s) | 64 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 48, 49, 90, 210, 340 |
| BseMII | CTCAG | 1 cut(s) | 541 |
| BseNI | ACTGG | 1 cut(s) | 34 |
| BseSI | GKGCMC | 3 cut(s) | 56, 409, 551 |
| BseXI | GCAGC | 1 cut(s) | 487 |
| Bsh1236I | CGCG | 3 cut(s) | 91, 191, 312 |
| BshFI | GGCC | 6 cut(s) | 54, 109, 220, 235, 407, 549 |
| BsiHKAI | GWGCWC | 2 cut(s) | 21, 319 |
| BsiHKCI | CYCGRG | 1 cut(s) | 56 |
| BsiSI | CCGG | 2 cut(s) | 57, 106 |
| BslFI | GGGAC | 1 cut(s) | 58 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 48, 49, 90, 210, 340 |
| BsmAI | GTCTC | 1 cut(s) | 91 |
| BsmFI | GGGAC | 1 cut(s) | 58 |
| BsnI | GGCC | 6 cut(s) | 54, 109, 220, 235, 407, 549 |
| BsoBI | CYCGRG | 1 cut(s) | 56 |
| Bsp120I | GGGCCC | 3 cut(s) | 52, 405, 547 |
| Bsp1286I | GDGCHC | 6 cut(s) | 21, 56, 64, 319, 409, 551 |
| BspANI | GGCC | 6 cut(s) | 54, 109, 220, 235, 407, 549 |
| BspCNI | CTCAG | 1 cut(s) | 540 |
| BspFNI | CGCG | 3 cut(s) | 91, 191, 312 |
| BspLI | GGNNCC | 5 cut(s) | 53, 54, 55, 407, 549 |
| BspMI | ACCTGC | 1 cut(s) | 366 |
| BsrFI | RCCGGY | 1 cut(s) | 105 |
| BsrI | ACTGG | 1 cut(s) | 34 |
| BssAI | RCCGGY | 1 cut(s) | 105 |
| BssECI | CCNNGG | 5 cut(s) | 55, 56, 57, 479, 551 |
| Bst2UI | CCWGG | 3 cut(s) | 130, 480, 541 |
| Bst4CI | ACNGT | 2 cut(s) | 16, 357 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 323 |
| BstC8I | GCNNGC | 2 cut(s) | 111, 304 |
| BstDEI | CTNAG | 1 cut(s) | 527 |
| BstDSI | CCRYGG | 1 cut(s) | 551 |
| BstEII | GGTNACC | 1 cut(s) | 351 |
| BstF5I | GGATG | 1 cut(s) | 64 |
| BstFNI | CGCG | 3 cut(s) | 91, 191, 312 |
| BstHHI | GCGC | 1 cut(s) | 191 |
| BstMAI | GTCTC | 1 cut(s) | 91 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 277, 323, 381 |
| BstNI | CCWGG | 3 cut(s) | 130, 480, 541 |
| BstPI | GGTNACC | 1 cut(s) | 351 |
| BstSCI | CCNGG | 5 cut(s) | 55, 56, 128, 478, 539 |
| BstSLI | GKGCMC | 3 cut(s) | 56, 409, 551 |
| BstUI | CGCG | 3 cut(s) | 91, 191, 312 |
| BstV1I | GCAGC | 1 cut(s) | 487 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 471 |
| BsuRI | GGCC | 6 cut(s) | 54, 109, 220, 235, 407, 549 |
| BtgI | CCRYGG | 1 cut(s) | 551 |
| BtsCI | GGATG | 1 cut(s) | 64 |
| BtsIMutI | CAGTG | 2 cut(s) | 238, 535 |
| BveI | ACCTGC | 1 cut(s) | 366 |
| Cac8I | GCNNGC | 2 cut(s) | 111, 304 |
| CfoI | GCGC | 1 cut(s) | 191 |
| Cfr10I | RCCGGY | 1 cut(s) | 105 |
| Cfr13I | GGNCC | 9 cut(s) | 52, 53, 126, 132, 219, 405, 406, 547, 548 |
| Cfr9I | CCCGGG | 1 cut(s) | 56 |
| Csp6I | GTAC | 1 cut(s) | 358 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 398, 433 |
| CviQI | GTAC | 1 cut(s) | 358 |
| DdeI | CTNAG | 1 cut(s) | 527 |
| EaeI | YGGCCR | 2 cut(s) | 107, 233 |
| EciI | GGCGGA | 4 cut(s) | 109, 229, 359, 460 |
| Eco24I | GRGCYC | 4 cut(s) | 56, 64, 409, 551 |
| Eco47I | GGWCC | 2 cut(s) | 126, 132 |
| Eco57I | CTGAAG | 1 cut(s) | 512 |
| Eco88I | CYCGRG | 1 cut(s) | 56 |
| Eco91I | GGTNACC | 1 cut(s) | 351 |
| EcoO109I | RGGNCCY | 4 cut(s) | 52, 53, 132, 547 |
| EcoO65I | GGTNACC | 1 cut(s) | 351 |
| EcoRII | CCWGG | 3 cut(s) | 128, 478, 539 |
| EcoT38I | GRGCYC | 4 cut(s) | 56, 64, 409, 551 |
| FaiI | YATR | 3 cut(s) | 145, 149, 367 |
| FaqI | GGGAC | 1 cut(s) | 58 |
| FauI | CCCGC | 2 cut(s) | 83, 430 |
| Fnu4HI | GCNGC | 3 cut(s) | 92, 140, 476 |
| FokI | GGATG | 1 cut(s) | 51 |
| FriOI | GRGCYC | 4 cut(s) | 56, 64, 409, 551 |
| Fsp4HI | GCNGC | 3 cut(s) | 92, 140, 476 |
| FspBI | CTAG | 2 cut(s) | 44, 274 |
| GlaI | GCGC | 1 cut(s) | 190 |
| GluI | GCNGC | 3 cut(s) | 92, 140, 476 |
| GsuI | CTGGAG | 1 cut(s) | 17 |
| HaeIII | GGCC | 6 cut(s) | 54, 109, 220, 235, 407, 549 |
| HapII | CCGG | 2 cut(s) | 57, 106 |
| HhaI | GCGC | 1 cut(s) | 191 |
| Hin6I | GCGC | 1 cut(s) | 189 |
| HinP1I | GCGC | 1 cut(s) | 189 |
| HincII | GTYRAC | 2 cut(s) | 12, 331 |
| HindII | GTYRAC | 2 cut(s) | 12, 331 |
| HinfI | GANTC | 2 cut(s) | 99, 448 |
| HpaI | GTTAAC | 1 cut(s) | 12 |
| HpaII | CCGG | 2 cut(s) | 57, 106 |
| Hpy166II | GTNNAC | 3 cut(s) | 12, 39, 331 |
| Hpy8I | GTNNAC | 3 cut(s) | 12, 39, 331 |
| Hpy99I | CGWCG | 1 cut(s) | 259 |
| HpyAV | CCTTC | 2 cut(s) | 204, 487 |
| HpyCH4III | ACNGT | 2 cut(s) | 16, 357 |
| HpyCH4V | TGCA | 2 cut(s) | 326, 375 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 277, 323, 381 |
| HpyF3I | CTNAG | 1 cut(s) | 527 |
| HspAI | GCGC | 1 cut(s) | 189 |
| KspAI | GTTAAC | 1 cut(s) | 12 |
| LmnI | GCTCC | 2 cut(s) | 186, 314 |
| Lsp1109I | GCAGC | 1 cut(s) | 487 |
| LweI | GCATC | 3 cut(s) | 166, 313, 362 |
| MaeI | CTAG | 2 cut(s) | 44, 274 |
| MaeIII | GTNAC | 1 cut(s) | 351 |
| MhlI | GDGCHC | 6 cut(s) | 21, 56, 64, 319, 409, 551 |
| MlsI | TGGCCA | 1 cut(s) | 235 |
| MluCI | AATT | 1 cut(s) | 259 |
| MluNI | TGGCCA | 1 cut(s) | 235 |
| MlyI | GAGTC | 1 cut(s) | 93 |
| MnlI | CCTC | 5 cut(s) | 145, 210, 270, 422, 435 |
| Mox20I | TGGCCA | 1 cut(s) | 235 |
| MscI | TGGCCA | 1 cut(s) | 235 |
| MseI | TTAA | 3 cut(s) | 5, 11, 262 |
| MslI | CAYNNNNRTG | 2 cut(s) | 241, 370 |
| Msp20I | TGGCCA | 1 cut(s) | 235 |
| MspA1I | CMGCKG | 1 cut(s) | 78 |
| MspI | CCGG | 2 cut(s) | 57, 106 |
| MspR9I | CCNGG | 5 cut(s) | 57, 58, 130, 480, 541 |
| MvaI | CCWGG | 3 cut(s) | 130, 480, 541 |
| MvnI | CGCG | 3 cut(s) | 91, 191, 312 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 277, 323, 381 |
| NciI | CCSGG | 2 cut(s) | 57, 58 |
| NlaIV | GGNNCC | 5 cut(s) | 53, 54, 55, 407, 549 |
| PfeI | GAWTC | 1 cut(s) | 448 |
| PfoI | TCCNGGA | 1 cut(s) | 128 |
| PkrI | GCNGC | 3 cut(s) | 93, 141, 477 |
| PleI | GAGTC | 1 cut(s) | 93 |
| PpsI | GAGTC | 1 cut(s) | 93 |
| PpuMI | RGGWCCY | 1 cut(s) | 132 |
| Psp5II | RGGWCCY | 1 cut(s) | 132 |
| Psp6I | CCWGG | 3 cut(s) | 128, 478, 539 |
| PspEI | GGTNACC | 1 cut(s) | 351 |
| PspGI | CCWGG | 3 cut(s) | 128, 478, 539 |
| PspN4I | GGNNCC | 5 cut(s) | 53, 54, 55, 407, 549 |
| PspOMI | GGGCCC | 3 cut(s) | 52, 405, 547 |
| PspPI | GGNCC | 9 cut(s) | 52, 53, 126, 132, 219, 405, 406, 547, 548 |
| PspPPI | RGGWCCY | 1 cut(s) | 132 |
| RsaI | GTAC | 1 cut(s) | 359 |
| RsaNI | GTAC | 1 cut(s) | 358 |
| RseI | CAYNNNNRTG | 2 cut(s) | 241, 370 |
| SaqAI | TTAA | 3 cut(s) | 5, 11, 262 |
| SatI | GCNGC | 3 cut(s) | 92, 140, 476 |
| Sau96I | GGNCC | 9 cut(s) | 52, 53, 126, 132, 219, 405, 406, 547, 548 |
| SchI | GAGTC | 1 cut(s) | 93 |
| ScrFI | CCNGG | 5 cut(s) | 57, 58, 130, 480, 541 |
| SduI | GDGCHC | 6 cut(s) | 21, 56, 64, 319, 409, 551 |
| SetI | ASST | 6 cut(s) | 128, 137, 380, 414, 498, 507 |
| SfaNI | GCATC | 3 cut(s) | 166, 313, 362 |
| SinI | GGWCC | 2 cut(s) | 126, 132 |
| SmaI | CCCGGG | 1 cut(s) | 58 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 241, 370 |
| Sse9I | AATT | 1 cut(s) | 259 |
| SspMI | CTAG | 2 cut(s) | 44, 274 |
| StyD4I | CCNGG | 5 cut(s) | 55, 56, 128, 478, 539 |
| TaaI | ACNGT | 2 cut(s) | 16, 357 |
| TaqI | TCGA | 1 cut(s) | 254 |
| TasI | AATT | 1 cut(s) | 259 |
| TauI | GCSGC | 2 cut(s) | 94, 142 |
| TfiI | GAWTC | 1 cut(s) | 448 |
| Tru1I | TTAA | 3 cut(s) | 5, 11, 262 |
| Tru9I | TTAA | 3 cut(s) | 5, 11, 262 |
| TscAI | CASTG | 2 cut(s) | 245, 535 |
| TseI | GCWGC | 1 cut(s) | 475 |
| TspMI | CCCGGG | 1 cut(s) | 56 |
| TspRI | CASTG | 2 cut(s) | 245, 535 |
| VpaK11BI | GGWCC | 2 cut(s) | 126, 132 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 519 |
| XmaI | CCCGGG | 1 cut(s) | 56 |
| XspI | CTAG | 2 cut(s) | 44, 274 |