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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 145 |
| AciI | CCGC | 3 cut(s) | 30, 48, 117 |
| AjnI | CCWGG | 3 cut(s) | 10, 22, 82 |
| AluBI | AGCT | 1 cut(s) | 107 |
| AluI | AGCT | 1 cut(s) | 107 |
| AoxI | GGCC | 3 cut(s) | 80, 166, 172 |
| ApeKI | GCWGC | 2 cut(s) | 45, 93 |
| AspLEI | GCGC | 2 cut(s) | 145, 157 |
| AspS9I | GGNCC | 4 cut(s) | 26, 86, 119, 173 |
| AvaII | GGWCC | 3 cut(s) | 26, 86, 119 |
| BanII | GRGCYC | 1 cut(s) | 18 |
| BbvI | GCAGC | 2 cut(s) | 57, 80 |
| BccI | CCATC | 1 cut(s) | 83 |
| BciT130I | CCWGG | 3 cut(s) | 12, 24, 84 |
| BfmI | CTRYAG | 1 cut(s) | 43 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 79 |
| BisI | GCNGC | 2 cut(s) | 46, 94 |
| BlsI | GCNGC | 2 cut(s) | 47, 95 |
| Bme1390I | CCNGG | 3 cut(s) | 12, 24, 84 |
| Bme18I | GGWCC | 3 cut(s) | 26, 86, 119 |
| BmgT120I | GGNCC | 4 cut(s) | 26, 86, 119, 173 |
| BmiI | GGNNCC | 2 cut(s) | 7, 28 |
| BmrFI | CCNGG | 3 cut(s) | 12, 24, 84 |
| BmsI | GCATC | 1 cut(s) | 73 |
| BsaJI | CCNNGG | 4 cut(s) | 10, 11, 22, 169 |
| BseBI | CCWGG | 3 cut(s) | 12, 24, 84 |
| BseDI | CCNNGG | 4 cut(s) | 10, 11, 22, 169 |
| BseGI | GGATG | 1 cut(s) | 18 |
| BseXI | GCAGC | 2 cut(s) | 57, 80 |
| Bsh1236I | CGCG | 1 cut(s) | 145 |
| BshFI | GGCC | 3 cut(s) | 82, 168, 174 |
| BslFI | GGGAC | 1 cut(s) | 12 |
| BsmFI | GGGAC | 1 cut(s) | 12 |
| BsnI | GGCC | 3 cut(s) | 82, 168, 174 |
| Bsp1286I | GDGCHC | 1 cut(s) | 18 |
| BspACI | CCGC | 3 cut(s) | 30, 48, 117 |
| BspANI | GGCC | 3 cut(s) | 82, 168, 174 |
| BspFNI | CGCG | 1 cut(s) | 145 |
| BspLI | GGNNCC | 2 cut(s) | 7, 28 |
| BspMAI | CTGCAG | 1 cut(s) | 47 |
| BssECI | CCNNGG | 4 cut(s) | 10, 11, 22, 169 |
| Bst2UI | CCWGG | 3 cut(s) | 12, 24, 84 |
| BstC8I | GCNNGC | 1 cut(s) | 109 |
| BstF5I | GGATG | 1 cut(s) | 18 |
| BstFNI | CGCG | 1 cut(s) | 145 |
| BstHHI | GCGC | 2 cut(s) | 145, 157 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 51, 70, 79 |
| BstNI | CCWGG | 3 cut(s) | 12, 24, 84 |
| BstSCI | CCNGG | 3 cut(s) | 10, 22, 82 |
| BstSFI | CTRYAG | 1 cut(s) | 43 |
| BstUI | CGCG | 1 cut(s) | 145 |
| BstV1I | GCAGC | 2 cut(s) | 57, 80 |
| BsuRI | GGCC | 3 cut(s) | 82, 168, 174 |
| BtsCI | GGATG | 1 cut(s) | 18 |
| Cac8I | GCNNGC | 1 cut(s) | 109 |
| CfoI | GCGC | 2 cut(s) | 145, 157 |
| Cfr13I | GGNCC | 4 cut(s) | 26, 86, 119, 173 |
| CviJI | RGCY | 5 cut(s) | 16, 82, 107, 168, 174 |
| CviKI_1 | RGCY | 5 cut(s) | 16, 82, 107, 168, 174 |
| EciI | GGCGGA | 1 cut(s) | 132 |
| Eco24I | GRGCYC | 1 cut(s) | 18 |
| Eco47I | GGWCC | 3 cut(s) | 26, 86, 119 |
| EcoO109I | RGGNCCY | 2 cut(s) | 26, 86 |
| EcoRII | CCWGG | 3 cut(s) | 10, 22, 82 |
| EcoT38I | GRGCYC | 1 cut(s) | 18 |
| FaiI | YATR | 3 cut(s) | 99, 103, 159 |
| FaqI | GGGAC | 1 cut(s) | 12 |
| FauI | CCCGC | 1 cut(s) | 37 |
| Fnu4HI | GCNGC | 2 cut(s) | 46, 94 |
| FokI | GGATG | 1 cut(s) | 5 |
| FriOI | GRGCYC | 1 cut(s) | 18 |
| Fsp4HI | GCNGC | 2 cut(s) | 46, 94 |
| GlaI | GCGC | 2 cut(s) | 144, 156 |
| GluI | GCNGC | 2 cut(s) | 46, 94 |
| HaeIII | GGCC | 3 cut(s) | 82, 168, 174 |
| HhaI | GCGC | 2 cut(s) | 145, 157 |
| Hin6I | GCGC | 2 cut(s) | 143, 155 |
| HinP1I | GCGC | 2 cut(s) | 143, 155 |
| HpyAV | CCTTC | 2 cut(s) | 132, 158 |
| HpyCH4V | TGCA | 1 cut(s) | 45 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 51, 70, 79 |
| HspAI | GCGC | 2 cut(s) | 143, 155 |
| LmnI | GCTCC | 1 cut(s) | 140 |
| LpnPI | CCDG | 7 cut(s) | 9, 24, 36, 64, 69, 93, 96 |
| Lsp1109I | GCAGC | 2 cut(s) | 57, 80 |
| LweI | GCATC | 1 cut(s) | 73 |
| MhlI | GDGCHC | 1 cut(s) | 18 |
| MluCI | AATT | 1 cut(s) | 179 |
| MnlI | CCTC | 3 cut(s) | 44, 99, 164 |
| MspA1I | CMGCKG | 2 cut(s) | 32, 48 |
| MspR9I | CCNGG | 3 cut(s) | 12, 24, 84 |
| MvaI | CCWGG | 3 cut(s) | 12, 24, 84 |
| MvnI | CGCG | 1 cut(s) | 145 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 51, 70, 79 |
| NlaIV | GGNNCC | 2 cut(s) | 7, 28 |
| PasI | CCCWGGG | 1 cut(s) | 11 |
| PkrI | GCNGC | 2 cut(s) | 47, 95 |
| PpuMI | RGGWCCY | 2 cut(s) | 26, 86 |
| Psp5II | RGGWCCY | 2 cut(s) | 26, 86 |
| Psp6I | CCWGG | 3 cut(s) | 10, 22, 82 |
| PspGI | CCWGG | 3 cut(s) | 10, 22, 82 |
| PspN4I | GGNNCC | 2 cut(s) | 7, 28 |
| PspPI | GGNCC | 4 cut(s) | 26, 86, 119, 173 |
| PspPPI | RGGWCCY | 2 cut(s) | 26, 86 |
| PstI | CTGCAG | 1 cut(s) | 47 |
| SatI | GCNGC | 2 cut(s) | 46, 94 |
| Sau96I | GGNCC | 4 cut(s) | 26, 86, 119, 173 |
| ScrFI | CCNGG | 3 cut(s) | 12, 24, 84 |
| SduI | GDGCHC | 1 cut(s) | 18 |
| SetI | ASST | 4 cut(s) | 28, 91, 109, 124 |
| SfaNI | GCATC | 1 cut(s) | 73 |
| SfcI | CTRYAG | 1 cut(s) | 43 |
| SinI | GGWCC | 3 cut(s) | 26, 86, 119 |
| Sse9I | AATT | 1 cut(s) | 179 |
| SsiI | CCGC | 3 cut(s) | 30, 48, 117 |
| StyD4I | CCNGG | 3 cut(s) | 10, 22, 82 |
| TasI | AATT | 1 cut(s) | 179 |
| TseI | GCWGC | 2 cut(s) | 45, 93 |
| VpaK11BI | GGWCC | 3 cut(s) | 26, 86, 119 |