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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 508 |
| AccB1I | GGYRCC | 1 cut(s) | 620 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 94, 571 |
| AccBSI | CCGCTC | 1 cut(s) | 465 |
| AccI | GTMKAC | 1 cut(s) | 415 |
| AclWI | GGATC | 1 cut(s) | 258 |
| AcvI | CACGTG | 1 cut(s) | 59 |
| AcyI | GRCGYC | 3 cut(s) | 65, 503, 621 |
| AfaI | GTAC | 1 cut(s) | 233 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 13, 94, 208, 425, 480, 571, 663 |
| AjnI | CCWGG | 4 cut(s) | 6, 404, 473, 661 |
| AluBI | AGCT | 2 cut(s) | 365, 692 |
| AluI | AGCT | 2 cut(s) | 365, 692 |
| AlwI | GGATC | 1 cut(s) | 258 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 94 |
| AoxI | GGCC | 5 cut(s) | 39, 165, 211, 303, 665 |
| ApaI | GGGCCC | 1 cut(s) | 669 |
| ApeKI | GCWGC | 3 cut(s) | 195, 198, 677 |
| AspLEI | GCGC | 5 cut(s) | 382, 431, 513, 623, 631 |
| AspS9I | GGNCC | 6 cut(s) | 40, 211, 304, 520, 665, 666 |
| AsuC2I | CCSGG | 4 cut(s) | 174, 210, 420, 608 |
| AsuHPI | GGTGA | 1 cut(s) | 664 |
| AvaII | GGWCC | 1 cut(s) | 520 |
| BaeGI | GKGCMC | 1 cut(s) | 669 |
| BanI | GGYRCC | 1 cut(s) | 620 |
| BanII | GRGCYC | 1 cut(s) | 669 |
| BbrPI | CACGTG | 1 cut(s) | 59 |
| BbvCI | CCTCAGC | 1 cut(s) | 300 |
| BbvI | GCAGC | 3 cut(s) | 207, 210, 664 |
| BciT130I | CCWGG | 4 cut(s) | 8, 406, 475, 663 |
| BcnI | CCSGG | 4 cut(s) | 174, 210, 420, 608 |
| BfaI | CTAG | 2 cut(s) | 239, 245 |
| BfoI | RGCGCY | 2 cut(s) | 624, 632 |
| BfuAI | ACCTGC | 1 cut(s) | 508 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 210 |
| BglII | AGATCT | 1 cut(s) | 217 |
| BisI | GCNGC | 9 cut(s) | 25, 39, 53, 63, 196, 199, 202, 452, 678 |
| BlsI | GCNGC | 9 cut(s) | 26, 40, 54, 64, 197, 200, 203, 453, 679 |
| Bme1390I | CCNGG | 8 cut(s) | 8, 174, 210, 406, 420, 475, 608, 663 |
| Bme18I | GGWCC | 1 cut(s) | 520 |
| BmgT120I | GGNCC | 6 cut(s) | 40, 211, 304, 520, 665, 666 |
| BmiI | GGNNCC | 2 cut(s) | 622, 667 |
| BmrFI | CCNGG | 8 cut(s) | 8, 174, 210, 406, 420, 475, 608, 663 |
| BmsI | GCATC | 1 cut(s) | 570 |
| BpmI | CTGGAG | 2 cut(s) | 71, 611 |
| Bpu10I | CCTNAGC | 1 cut(s) | 300 |
| BpuMI | CCSGG | 4 cut(s) | 174, 210, 420, 608 |
| BsaAI | YACGTR | 2 cut(s) | 59, 398 |
| BsaHI | GRCGYC | 3 cut(s) | 65, 503, 621 |
| BsaJI | CCNNGG | 5 cut(s) | 6, 172, 474, 506, 662 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 584, 614 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 13, 94, 208, 425, 480, 571, 663 |
| Bse118I | RCCGGY | 1 cut(s) | 548 |
| Bse1I | ACTGG | 2 cut(s) | 430, 594 |
| Bse3DI | GCAATG | 1 cut(s) | 559 |
| BseBI | CCWGG | 4 cut(s) | 8, 406, 475, 663 |
| BseDI | CCNNGG | 5 cut(s) | 6, 172, 474, 506, 662 |
| BseGI | GGATG | 1 cut(s) | 585 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 13, 94, 208, 425, 480, 571, 663 |
| BseMI | GCAATG | 1 cut(s) | 559 |
| BseMII | CTCAG | 3 cut(s) | 291, 477, 650 |
| BseNI | ACTGG | 2 cut(s) | 430, 594 |
| BseRI | GAGGAG | 1 cut(s) | 293 |
| BseSI | GKGCMC | 1 cut(s) | 669 |
| BseXI | GCAGC | 3 cut(s) | 207, 210, 664 |
| BseYI | CCCAGC | 2 cut(s) | 94, 625 |
| BshFI | GGCC | 5 cut(s) | 41, 167, 213, 305, 667 |
| BshNI | GGYRCC | 1 cut(s) | 620 |
| BsiSI | CCGG | 5 cut(s) | 174, 209, 420, 549, 607 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 13, 94, 208, 425, 480, 571, 663 |
| BsnI | GGCC | 5 cut(s) | 41, 167, 213, 305, 667 |
| Bsp120I | GGGCCC | 1 cut(s) | 665 |
| Bsp1286I | GDGCHC | 1 cut(s) | 669 |
| Bsp143I | GATC | 3 cut(s) | 138, 217, 250 |
| BspANI | GGCC | 5 cut(s) | 41, 167, 213, 305, 667 |
| BspCNI | CTCAG | 3 cut(s) | 292, 478, 649 |
| BspLI | GGNNCC | 2 cut(s) | 622, 667 |
| BspMI | ACCTGC | 1 cut(s) | 508 |
| BspPI | GGATC | 1 cut(s) | 258 |
| BspT107I | GGYRCC | 1 cut(s) | 620 |
| BsrBI | CCGCTC | 1 cut(s) | 465 |
| BsrDI | GCAATG | 1 cut(s) | 559 |
| BsrFI | RCCGGY | 1 cut(s) | 548 |
| BsrI | ACTGG | 2 cut(s) | 430, 594 |
| BssAI | RCCGGY | 1 cut(s) | 548 |
| BssECI | CCNNGG | 5 cut(s) | 6, 172, 474, 506, 662 |
| BssMI | GATC | 3 cut(s) | 138, 217, 250 |
| BssNI | GRCGYC | 3 cut(s) | 65, 503, 621 |
| Bst2UI | CCWGG | 4 cut(s) | 8, 406, 475, 663 |
| Bst4CI | ACNGT | 2 cut(s) | 315, 393 |
| Bst6I | CTCTTC | 1 cut(s) | 152 |
| BstACI | GRCGYC | 3 cut(s) | 65, 503, 621 |
| BstBAI | YACGTR | 2 cut(s) | 59, 398 |
| BstC8I | GCNNGC | 4 cut(s) | 22, 515, 550, 690 |
| BstDEI | CTNAG | 3 cut(s) | 300, 486, 636 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 11 |
| BstF5I | GGATG | 1 cut(s) | 585 |
| BstH2I | RGCGCY | 2 cut(s) | 624, 632 |
| BstHHI | GCGC | 5 cut(s) | 382, 431, 513, 623, 631 |
| BstKTI | GATC | 3 cut(s) | 141, 220, 253 |
| BstMBI | GATC | 3 cut(s) | 138, 217, 250 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 201, 210, 377, 437, 523, 554 |
| BstNI | CCWGG | 4 cut(s) | 8, 406, 475, 663 |
| BstSCI | CCNGG | 8 cut(s) | 6, 172, 208, 404, 418, 473, 606, 661 |
| BstSLI | GKGCMC | 1 cut(s) | 669 |
| BstV1I | GCAGC | 3 cut(s) | 207, 210, 664 |
| BstX2I | RGATCY | 2 cut(s) | 217, 250 |
| BstYI | RGATCY | 2 cut(s) | 217, 250 |
| BsuRI | GGCC | 5 cut(s) | 41, 167, 213, 305, 667 |
| BtsCI | GGATG | 1 cut(s) | 585 |
| BtsI | GCAGTG | 1 cut(s) | 446 |
| BtsIMutI | CAGTG | 2 cut(s) | 423, 446 |
| BveI | ACCTGC | 1 cut(s) | 508 |
| Cac8I | GCNNGC | 4 cut(s) | 22, 515, 550, 690 |
| CaiI | CAGNNNCTG | 1 cut(s) | 94 |
| CfoI | GCGC | 5 cut(s) | 382, 431, 513, 623, 631 |
| Cfr10I | RCCGGY | 1 cut(s) | 548 |
| Cfr13I | GGNCC | 6 cut(s) | 40, 211, 304, 520, 665, 666 |
| CseI | GACGC | 5 cut(s) | 54, 65, 100, 364, 511 |
| Csp6I | GTAC | 1 cut(s) | 232 |
| CviQI | GTAC | 1 cut(s) | 232 |
| DdeI | CTNAG | 3 cut(s) | 300, 486, 636 |
| DinI | GGCGCC | 1 cut(s) | 622 |
| DpnI | GATC | 3 cut(s) | 140, 219, 252 |
| DpnII | GATC | 3 cut(s) | 138, 217, 250 |
| Eam1104I | CTCTTC | 1 cut(s) | 152 |
| EarI | CTCTTC | 1 cut(s) | 152 |
| EciI | GGCGGA | 2 cut(s) | 486, 626 |
| Eco24I | GRGCYC | 1 cut(s) | 669 |
| Eco47I | GGWCC | 1 cut(s) | 520 |
| Eco72I | CACGTG | 1 cut(s) | 59 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 11 |
| EcoO109I | RGGNCCY | 1 cut(s) | 666 |
| EcoRII | CCWGG | 4 cut(s) | 6, 404, 473, 661 |
| EcoT38I | GRGCYC | 1 cut(s) | 669 |
| EgeI | GGCGCC | 1 cut(s) | 622 |
| EheI | GGCGCC | 1 cut(s) | 622 |
| FaiI | YATR | 6 cut(s) | 157, 159, 230, 258, 706, 708 |
| FauNDI | CATATG | 1 cut(s) | 706 |
| FblI | GTMKAC | 1 cut(s) | 415 |
| Fnu4HI | GCNGC | 9 cut(s) | 25, 39, 53, 63, 196, 199, 202, 452, 678 |
| FokI | GGATG | 1 cut(s) | 592 |
| FriOI | GRGCYC | 1 cut(s) | 669 |
| Fsp4HI | GCNGC | 9 cut(s) | 25, 39, 53, 63, 196, 199, 202, 452, 678 |
| FspBI | CTAG | 2 cut(s) | 239, 245 |
| GlaI | GCGC | 5 cut(s) | 381, 430, 512, 622, 630 |
| GluI | GCNGC | 9 cut(s) | 25, 39, 53, 63, 196, 199, 202, 452, 678 |
| GsaI | CCCAGC | 2 cut(s) | 98, 629 |
| GsuI | CTGGAG | 2 cut(s) | 71, 611 |
| HaeII | RGCGCY | 2 cut(s) | 624, 632 |
| HaeIII | GGCC | 5 cut(s) | 41, 167, 213, 305, 667 |
| HapII | CCGG | 5 cut(s) | 174, 209, 420, 549, 607 |
| HgaI | GACGC | 5 cut(s) | 54, 65, 100, 364, 511 |
| HhaI | GCGC | 5 cut(s) | 382, 431, 513, 623, 631 |
| Hin1I | GRCGYC | 3 cut(s) | 65, 503, 621 |
| Hin6I | GCGC | 5 cut(s) | 380, 429, 511, 621, 629 |
| HinP1I | GCGC | 5 cut(s) | 380, 429, 511, 621, 629 |
| HincII | GTYRAC | 1 cut(s) | 416 |
| HindII | GTYRAC | 1 cut(s) | 416 |
| HinfI | GANTC | 5 cut(s) | 99, 443, 614, 640, 682 |
| HpaII | CCGG | 5 cut(s) | 174, 209, 420, 549, 607 |
| HphI | GGTGA | 1 cut(s) | 664 |
| Hpy166II | GTNNAC | 1 cut(s) | 416 |
| Hpy188I | TCNGA | 5 cut(s) | 81, 125, 469, 639, 698 |
| Hpy188III | TCNNGA | 3 cut(s) | 69, 88, 657 |
| Hpy8I | GTNNAC | 1 cut(s) | 416 |
| Hpy99I | CGWCG | 1 cut(s) | 505 |
| HpyAV | CCTTC | 1 cut(s) | 679 |
| HpyCH4III | ACNGT | 2 cut(s) | 315, 393 |
| HpyCH4IV | ACGT | 3 cut(s) | 58, 274, 397 |
| HpyCH4V | TGCA | 4 cut(s) | 557, 583, 688, 710 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 201, 210, 377, 437, 523, 554 |
| HpyF3I | CTNAG | 3 cut(s) | 300, 486, 636 |
| HpySE526I | ACGT | 3 cut(s) | 58, 274, 397 |
| Hsp92I | GRCGYC | 3 cut(s) | 65, 503, 621 |
| HspAI | GCGC | 5 cut(s) | 380, 429, 511, 621, 629 |
| KasI | GGCGCC | 1 cut(s) | 620 |
| KroI | GCCGGC | 1 cut(s) | 548 |
| KroNI | GCCGGC | 1 cut(s) | 550 |
| Kzo9I | GATC | 3 cut(s) | 138, 217, 250 |
| LmnI | GCTCC | 1 cut(s) | 592 |
| Lsp1109I | GCAGC | 3 cut(s) | 207, 210, 664 |
| LweI | GCATC | 1 cut(s) | 570 |
| MaeI | CTAG | 2 cut(s) | 239, 245 |
| MaeII | ACGT | 3 cut(s) | 58, 274, 397 |
| MaeIII | GTNAC | 2 cut(s) | 330, 393 |
| MalI | GATC | 3 cut(s) | 140, 219, 252 |
| MbiI | CCGCTC | 1 cut(s) | 465 |
| MboI | GATC | 3 cut(s) | 138, 217, 250 |
| MboII | GAAGA | 5 cut(s) | 139, 145, 148, 245, 733 |
| MflI | RGATCY | 2 cut(s) | 217, 250 |
| MhlI | GDGCHC | 1 cut(s) | 669 |
| Mly113I | GGCGCC | 1 cut(s) | 621 |
| MlyI | GAGTC | 1 cut(s) | 623 |
| MroNI | GCCGGC | 1 cut(s) | 548 |
| MseI | TTAA | 1 cut(s) | 45 |
| MslI | CAYNNNNRTG | 1 cut(s) | 227 |
| MspA1I | CMGCKG | 1 cut(s) | 677 |
| MspI | CCGG | 5 cut(s) | 174, 209, 420, 549, 607 |
| MspR9I | CCNGG | 8 cut(s) | 8, 174, 210, 406, 420, 475, 608, 663 |
| MvaI | CCWGG | 4 cut(s) | 8, 406, 475, 663 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 201, 210, 377, 437, 523, 554 |
| NaeI | GCCGGC | 1 cut(s) | 550 |
| NarI | GGCGCC | 1 cut(s) | 621 |
| NciI | CCSGG | 4 cut(s) | 174, 210, 420, 608 |
| NdeI | CATATG | 1 cut(s) | 706 |
| NdeII | GATC | 3 cut(s) | 138, 217, 250 |
| NgoMIV | GCCGGC | 1 cut(s) | 548 |
| NlaIV | GGNNCC | 2 cut(s) | 622, 667 |
| NmeAIII | GCCGAG | 1 cut(s) | 531 |
| NmuCI | GTSAC | 2 cut(s) | 330, 393 |
| PdiI | GCCGGC | 1 cut(s) | 550 |
| PfeI | GAWTC | 4 cut(s) | 99, 443, 640, 682 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 94, 571 |
| PkrI | GCNGC | 9 cut(s) | 26, 40, 54, 64, 197, 200, 203, 453, 679 |
| PleI | GAGTC | 1 cut(s) | 622 |
| PluTI | GGCGCC | 1 cut(s) | 624 |
| PmaCI | CACGTG | 1 cut(s) | 59 |
| PmlI | CACGTG | 1 cut(s) | 59 |
| PpsI | GAGTC | 1 cut(s) | 622 |
| Ppu21I | YACGTR | 2 cut(s) | 59, 398 |
| Psp6I | CCWGG | 4 cut(s) | 6, 404, 473, 661 |
| PspCI | CACGTG | 1 cut(s) | 59 |
| PspFI | CCCAGC | 2 cut(s) | 94, 625 |
| PspGI | CCWGG | 4 cut(s) | 6, 404, 473, 661 |
| PspN4I | GGNNCC | 2 cut(s) | 622, 667 |
| PspOMI | GGGCCC | 1 cut(s) | 665 |
| PspPI | GGNCC | 6 cut(s) | 40, 211, 304, 520, 665, 666 |
| PstNI | CAGNNNCTG | 1 cut(s) | 94 |
| PsuI | RGATCY | 2 cut(s) | 217, 250 |
| RsaI | GTAC | 1 cut(s) | 233 |
| RsaNI | GTAC | 1 cut(s) | 232 |
| RseI | CAYNNNNRTG | 1 cut(s) | 227 |
| SalI | GTCGAC | 1 cut(s) | 414 |
| SaqAI | TTAA | 1 cut(s) | 45 |
| SatI | GCNGC | 9 cut(s) | 25, 39, 53, 63, 196, 199, 202, 452, 678 |
| Sau3AI | GATC | 3 cut(s) | 138, 217, 250 |
| Sau96I | GGNCC | 6 cut(s) | 40, 211, 304, 520, 665, 666 |
| SchI | GAGTC | 1 cut(s) | 623 |
| ScrFI | CCNGG | 8 cut(s) | 8, 174, 210, 406, 420, 475, 608, 663 |
| SduI | GDGCHC | 1 cut(s) | 669 |
| SfaNI | GCATC | 1 cut(s) | 570 |
| SfoI | GGCGCC | 1 cut(s) | 622 |
| SinI | GGWCC | 1 cut(s) | 520 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 227 |
| SspDI | GGCGCC | 1 cut(s) | 620 |
| SspI | AATATT | 1 cut(s) | 532 |
| SspMI | CTAG | 2 cut(s) | 239, 245 |
| StyD4I | CCNGG | 8 cut(s) | 6, 172, 208, 404, 418, 473, 606, 661 |
| TaaI | ACNGT | 2 cut(s) | 315, 393 |
| TaiI | ACGT | 3 cut(s) | 61, 277, 400 |
| TaqI | TCGA | 3 cut(s) | 102, 415, 540 |
| TauI | GCSGC | 6 cut(s) | 27, 41, 55, 65, 204, 454 |
| TfiI | GAWTC | 4 cut(s) | 99, 443, 640, 682 |
| Tru1I | TTAA | 1 cut(s) | 45 |
| Tru9I | TTAA | 1 cut(s) | 45 |
| TscAI | CASTG | 2 cut(s) | 430, 453 |
| TseFI | GTSAC | 2 cut(s) | 330, 393 |
| TseI | GCWGC | 3 cut(s) | 195, 198, 677 |
| Tsp45I | GTSAC | 2 cut(s) | 330, 393 |
| TspDTI | ATGAA | 2 cut(s) | 245, 357 |
| TspRI | CASTG | 2 cut(s) | 430, 453 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 94, 571 |
| VpaK11BI | GGWCC | 1 cut(s) | 520 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 11 |
| XmiI | GTMKAC | 1 cut(s) | 415 |
| XspI | CTAG | 2 cut(s) | 239, 245 |