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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 463 |
| Acc36I | ACCTGC | 1 cut(s) | 463 |
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccII | CGCG | 2 cut(s) | 74, 90 |
| AclWI | GGATC | 3 cut(s) | 168, 201, 527 |
| AcyI | GRCGYC | 1 cut(s) | 380 |
| AfaI | GTAC | 2 cut(s) | 176, 408 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 685 |
| AhlI | ACTAGT | 1 cut(s) | 460 |
| AjnI | CCWGG | 6 cut(s) | 133, 156, 249, 347, 382, 512 |
| AluBI | AGCT | 1 cut(s) | 607 |
| AluI | AGCT | 1 cut(s) | 607 |
| Alw21I | GWGCWC | 1 cut(s) | 624 |
| Alw44I | GTGCAC | 1 cut(s) | 620 |
| AlwI | GGATC | 3 cut(s) | 168, 201, 527 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 640 |
| AoxI | GGCC | 6 cut(s) | 154, 246, 359, 386, 567, 576 |
| ApaLI | GTGCAC | 1 cut(s) | 620 |
| ApeKI | GCWGC | 5 cut(s) | 138, 141, 263, 305, 342 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 5 cut(s) | 92, 231, 268, 382, 552 |
| AspS9I | GGNCC | 4 cut(s) | 247, 360, 386, 440 |
| AsuC2I | CCSGG | 2 cut(s) | 320, 562 |
| AsuHPI | GGTGA | 5 cut(s) | 134, 160, 542, 602, 668 |
| AsuII | TTCGAA | 1 cut(s) | 282 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BaeGI | GKGCMC | 1 cut(s) | 624 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| Bbv12I | GWGCWC | 1 cut(s) | 624 |
| BbvCI | CCTCAGC | 2 cut(s) | 27, 243 |
| BbvI | GCAGC | 5 cut(s) | 150, 153, 250, 292, 354 |
| BccI | CCATC | 3 cut(s) | 175, 253, 370 |
| BciT130I | CCWGG | 6 cut(s) | 135, 158, 251, 349, 384, 514 |
| BcnI | CCSGG | 2 cut(s) | 320, 562 |
| BcuI | ACTAGT | 1 cut(s) | 460 |
| BfaI | CTAG | 3 cut(s) | 422, 461, 546 |
| BfoI | RGCGCY | 1 cut(s) | 383 |
| BfuAI | ACCTGC | 1 cut(s) | 463 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 153, 562, 575 |
| BisI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 446 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 344, 447 |
| Bme1390I | CCNGG | 8 cut(s) | 135, 158, 251, 320, 349, 384, 514, 562 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 360, 386, 440 |
| BmiI | GGNNCC | 3 cut(s) | 44, 381, 441 |
| BmrFI | CCNGG | 8 cut(s) | 135, 158, 251, 320, 349, 384, 514, 562 |
| BmsI | GCATC | 1 cut(s) | 583 |
| BpmI | CTGGAG | 1 cut(s) | 650 |
| Bpu10I | CCTNAGC | 2 cut(s) | 27, 243 |
| Bpu14I | TTCGAA | 1 cut(s) | 282 |
| BpuMI | CCSGG | 2 cut(s) | 320, 562 |
| BsaHI | GRCGYC | 1 cut(s) | 380 |
| BsaJI | CCNNGG | 4 cut(s) | 249, 383, 513, 545 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 685 |
| Bse118I | RCCGGY | 3 cut(s) | 368, 388, 587 |
| Bse1I | ACTGG | 3 cut(s) | 192, 570, 633 |
| BseBI | CCWGG | 6 cut(s) | 135, 158, 251, 349, 384, 514 |
| BseDI | CCNNGG | 4 cut(s) | 249, 383, 513, 545 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 685 |
| BseMII | CTCAG | 2 cut(s) | 18, 234 |
| BseNI | ACTGG | 3 cut(s) | 192, 570, 633 |
| BseRI | GAGGAG | 3 cut(s) | 44, 236, 239 |
| BseSI | GKGCMC | 1 cut(s) | 624 |
| BseXI | GCAGC | 5 cut(s) | 150, 153, 250, 292, 354 |
| BseYI | CCCAGC | 4 cut(s) | 106, 116, 362, 603 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 90 |
| Bsh1285I | CGRYCG | 1 cut(s) | 78 |
| BshFI | GGCC | 6 cut(s) | 156, 248, 361, 388, 569, 578 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 1 cut(s) | 78 |
| BsiHKAI | GWGCWC | 1 cut(s) | 624 |
| BsiSI | CCGG | 5 cut(s) | 320, 369, 389, 562, 588 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 685 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| BsnI | GGCC | 6 cut(s) | 156, 248, 361, 388, 569, 578 |
| Bsp119I | TTCGAA | 1 cut(s) | 282 |
| Bsp1286I | GDGCHC | 2 cut(s) | 45, 624 |
| Bsp143I | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BspANI | GGCC | 6 cut(s) | 156, 248, 361, 388, 569, 578 |
| BspCNI | CTCAG | 2 cut(s) | 19, 235 |
| BspFNI | CGCG | 2 cut(s) | 74, 90 |
| BspLI | GGNNCC | 3 cut(s) | 44, 381, 441 |
| BspMI | ACCTGC | 1 cut(s) | 463 |
| BspPI | GGATC | 3 cut(s) | 168, 201, 527 |
| BspT104I | TTCGAA | 1 cut(s) | 282 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrFI | RCCGGY | 3 cut(s) | 368, 388, 587 |
| BsrI | ACTGG | 3 cut(s) | 192, 570, 633 |
| BssAI | RCCGGY | 3 cut(s) | 368, 388, 587 |
| BssECI | CCNNGG | 4 cut(s) | 249, 383, 513, 545 |
| BssMI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BssNI | GRCGYC | 1 cut(s) | 380 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 6 cut(s) | 135, 158, 251, 349, 384, 514 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstACI | GRCGYC | 1 cut(s) | 380 |
| BstBI | TTCGAA | 1 cut(s) | 282 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 332, 554, 567 |
| BstDEI | CTNAG | 3 cut(s) | 27, 243, 525 |
| BstFNI | CGCG | 2 cut(s) | 74, 90 |
| BstH2I | RGCGCY | 1 cut(s) | 383 |
| BstHHI | GCGC | 5 cut(s) | 92, 231, 268, 382, 552 |
| BstKTI | GATC | 4 cut(s) | 78, 163, 196, 535 |
| BstMBI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BstMCI | CGRYCG | 1 cut(s) | 78 |
| BstNI | CCWGG | 6 cut(s) | 135, 158, 251, 349, 384, 514 |
| BstNSI | RCATGY | 1 cut(s) | 407 |
| BstSCI | CCNGG | 8 cut(s) | 133, 156, 249, 318, 347, 382, 512, 560 |
| BstSLI | GKGCMC | 1 cut(s) | 624 |
| BstUI | CGCG | 2 cut(s) | 74, 90 |
| BstV1I | GCAGC | 5 cut(s) | 150, 153, 250, 292, 354 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 6 cut(s) | 156, 248, 361, 388, 569, 578 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 463 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 332, 554, 567 |
| CaiI | CAGNNNCTG | 1 cut(s) | 640 |
| CfoI | GCGC | 5 cut(s) | 92, 231, 268, 382, 552 |
| Cfr10I | RCCGGY | 3 cut(s) | 368, 388, 587 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 360, 386, 440 |
| Csp6I | GTAC | 2 cut(s) | 175, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 3 cut(s) | 172, 271, 404 |
| CviQI | GTAC | 2 cut(s) | 175, 407 |
| DdeI | CTNAG | 3 cut(s) | 27, 243, 525 |
| DinI | GGCGCC | 1 cut(s) | 381 |
| DpnI | GATC | 4 cut(s) | 77, 162, 195, 534 |
| DpnII | GATC | 4 cut(s) | 75, 160, 193, 532 |
| EciI | GGCGGA | 4 cut(s) | 206, 494, 525, 574 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| EcoRII | CCWGG | 6 cut(s) | 133, 156, 249, 347, 382, 512 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| EgeI | GGCGCC | 1 cut(s) | 381 |
| EheI | GGCGCC | 1 cut(s) | 381 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| FaeI | CATG | 3 cut(s) | 175, 274, 407 |
| FaiI | YATR | 9 cut(s) | 173, 201, 272, 395, 405, 452, 542, 654, 656 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FatI | CATG | 3 cut(s) | 171, 270, 403 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| FauNDI | CATATG | 1 cut(s) | 654 |
| Fnu4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 446 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 446 |
| FspBI | CTAG | 3 cut(s) | 422, 461, 546 |
| GlaI | GCGC | 5 cut(s) | 91, 230, 267, 381, 551 |
| GluI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 446 |
| GsaI | CCCAGC | 4 cut(s) | 110, 120, 366, 607 |
| GsuI | CTGGAG | 1 cut(s) | 650 |
| HaeII | RGCGCY | 1 cut(s) | 383 |
| HaeIII | GGCC | 6 cut(s) | 156, 248, 361, 388, 569, 578 |
| HapII | CCGG | 5 cut(s) | 320, 369, 389, 562, 588 |
| HhaI | GCGC | 5 cut(s) | 92, 231, 268, 382, 552 |
| Hin1I | GRCGYC | 1 cut(s) | 380 |
| Hin1II | CATG | 3 cut(s) | 175, 274, 407 |
| Hin6I | GCGC | 5 cut(s) | 90, 229, 266, 380, 550 |
| HinP1I | GCGC | 5 cut(s) | 90, 229, 266, 380, 550 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 2 cut(s) | 482, 581 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 5 cut(s) | 320, 369, 389, 562, 588 |
| HphI | GGTGA | 5 cut(s) | 134, 160, 542, 602, 668 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 622 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 646 |
| Hpy188III | TCNNGA | 1 cut(s) | 432 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 622 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 277 |
| HpyCH4V | TGCA | 4 cut(s) | 403, 596, 622, 658 |
| HpyF3I | CTNAG | 3 cut(s) | 27, 243, 525 |
| HpySE526I | ACGT | 1 cut(s) | 277 |
| Hsp92I | GRCGYC | 1 cut(s) | 380 |
| Hsp92II | CATG | 3 cut(s) | 175, 274, 407 |
| HspAI | GCGC | 5 cut(s) | 90, 229, 266, 380, 550 |
| KasI | GGCGCC | 1 cut(s) | 379 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 4 cut(s) | 75, 160, 193, 532 |
| LmnI | GCTCC | 6 cut(s) | 31, 48, 214, 226, 469, 631 |
| Lsp1109I | GCAGC | 5 cut(s) | 150, 153, 250, 292, 354 |
| LweI | GCATC | 1 cut(s) | 583 |
| MaeI | CTAG | 3 cut(s) | 422, 461, 546 |
| MaeII | ACGT | 1 cut(s) | 277 |
| MaeIII | GTNAC | 3 cut(s) | 166, 336, 674 |
| MalI | GATC | 4 cut(s) | 77, 162, 195, 534 |
| MboI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| MboII | GAAGA | 2 cut(s) | 188, 296 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 2 cut(s) | 45, 624 |
| Mly113I | GGCGCC | 1 cut(s) | 380 |
| MmeI | TCCRAC | 3 cut(s) | 90, 447, 522 |
| MnlI | CCTC | 7 cut(s) | 22, 73, 118, 214, 217, 238, 630 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MseI | TTAA | 2 cut(s) | 84, 414 |
| MslI | CAYNNNNRTG | 2 cut(s) | 398, 507 |
| MspA1I | CMGCKG | 1 cut(s) | 120 |
| MspI | CCGG | 5 cut(s) | 320, 369, 389, 562, 588 |
| MspR9I | CCNGG | 8 cut(s) | 135, 158, 251, 320, 349, 384, 514, 562 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 6 cut(s) | 135, 158, 251, 349, 384, 514 |
| MvnI | CGCG | 2 cut(s) | 74, 90 |
| NarI | GGCGCC | 1 cut(s) | 380 |
| NciI | CCSGG | 2 cut(s) | 320, 562 |
| NdeI | CATATG | 1 cut(s) | 654 |
| NdeII | GATC | 4 cut(s) | 75, 160, 193, 532 |
| NlaIII | CATG | 3 cut(s) | 175, 274, 407 |
| NlaIV | GGNNCC | 3 cut(s) | 44, 381, 441 |
| NmuCI | GTSAC | 3 cut(s) | 166, 336, 674 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 1 cut(s) | 407 |
| NspV | TTCGAA | 1 cut(s) | 282 |
| PaqCI | CACCTGC | 1 cut(s) | 463 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 2 cut(s) | 482, 581 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 344, 447 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| PluTI | GGCGCC | 1 cut(s) | 383 |
| Psp6I | CCWGG | 6 cut(s) | 133, 156, 249, 347, 382, 512 |
| PspFI | CCCAGC | 4 cut(s) | 106, 116, 362, 603 |
| PspGI | CCWGG | 6 cut(s) | 133, 156, 249, 347, 382, 512 |
| PspN4I | GGNNCC | 3 cut(s) | 44, 381, 441 |
| PspPI | GGNCC | 4 cut(s) | 247, 360, 386, 440 |
| PstNI | CAGNNNCTG | 1 cut(s) | 640 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 2 cut(s) | 176, 408 |
| RsaNI | GTAC | 2 cut(s) | 175, 407 |
| RseI | CAYNNNNRTG | 2 cut(s) | 398, 507 |
| SaqAI | TTAA | 2 cut(s) | 84, 414 |
| SatI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 446 |
| Sau3AI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| Sau96I | GGNCC | 4 cut(s) | 247, 360, 386, 440 |
| ScrFI | CCNGG | 8 cut(s) | 135, 158, 251, 320, 349, 384, 514, 562 |
| SduI | GDGCHC | 2 cut(s) | 45, 624 |
| SetI | ASST | 5 cut(s) | 280, 477, 547, 609, 681 |
| SfaNI | GCATC | 1 cut(s) | 583 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 575 |
| SfoI | GGCGCC | 1 cut(s) | 381 |
| SfuI | TTCGAA | 1 cut(s) | 282 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 398, 507 |
| SpeI | ACTAGT | 1 cut(s) | 460 |
| SspDI | GGCGCC | 1 cut(s) | 379 |
| SspMI | CTAG | 3 cut(s) | 422, 461, 546 |
| StyD4I | CCNGG | 8 cut(s) | 133, 156, 249, 318, 347, 382, 512, 560 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 280 |
| TaqI | TCGA | 2 cut(s) | 282, 506 |
| TaqII | GACCGA | 1 cut(s) | 663 |
| TauI | GCSGC | 2 cut(s) | 147, 448 |
| TfiI | GAWTC | 2 cut(s) | 482, 581 |
| Tru1I | TTAA | 2 cut(s) | 84, 414 |
| Tru9I | TTAA | 2 cut(s) | 84, 414 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 3 cut(s) | 166, 336, 674 |
| TseI | GCWGC | 5 cut(s) | 138, 141, 263, 305, 342 |
| Tsp45I | GTSAC | 3 cut(s) | 166, 336, 674 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VneI | GTGCAC | 1 cut(s) | 620 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XceI | RCATGY | 1 cut(s) | 407 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 178 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 3 cut(s) | 422, 461, 546 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |