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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 379 |
| AccB1I | GGYRCC | 2 cut(s) | 365, 379 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 439 |
| AccBSI | CCGCTC | 1 cut(s) | 514 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AclWI | GGATC | 2 cut(s) | 201, 466 |
| AcoI | YGGCCR | 2 cut(s) | 48, 108 |
| AcuI | CTGAAG | 2 cut(s) | 48, 264 |
| AcyI | GRCGYC | 1 cut(s) | 489 |
| AfaI | GTAC | 2 cut(s) | 176, 381 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 151, 187, 256, 368, 439 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 3 cut(s) | 347, 382, 529 |
| Alw21I | GWGCWC | 1 cut(s) | 94 |
| Alw44I | GTGCAC | 1 cut(s) | 90 |
| AlwI | GGATC | 2 cut(s) | 201, 466 |
| AoxI | GGCC | 6 cut(s) | 48, 108, 154, 306, 386, 533 |
| ApaI | GGGCCC | 1 cut(s) | 537 |
| ApaLI | GTGCAC | 1 cut(s) | 90 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 545 |
| Asp718I | GGTACC | 1 cut(s) | 379 |
| AspLEI | GCGC | 3 cut(s) | 231, 403, 499 |
| AspS9I | GGNCC | 4 cut(s) | 154, 386, 533, 534 |
| AsuC2I | CCSGG | 5 cut(s) | 107, 117, 153, 363, 476 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 532 |
| BaeGI | GKGCMC | 2 cut(s) | 94, 537 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 363, 396 |
| BanI | GGYRCC | 2 cut(s) | 365, 379 |
| BanII | GRGCYC | 1 cut(s) | 537 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| Bbv12I | GWGCWC | 1 cut(s) | 94 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 532 |
| BccI | CCATC | 1 cut(s) | 59 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 410, 444 |
| BciT130I | CCWGG | 3 cut(s) | 349, 384, 531 |
| BcnI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 476 |
| BfaI | CTAG | 2 cut(s) | 182, 458 |
| BfmI | CTRYAG | 1 cut(s) | 554 |
| BfoI | RGCGCY | 1 cut(s) | 500 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 4 cut(s) | 139, 142, 145, 546 |
| BlsI | GCNGC | 4 cut(s) | 140, 143, 146, 547 |
| Bme1390I | CCNGG | 8 cut(s) | 107, 117, 153, 349, 363, 384, 476, 531 |
| BmgT120I | GGNCC | 4 cut(s) | 154, 386, 533, 534 |
| BmiI | GGNNCC | 3 cut(s) | 367, 381, 535 |
| BmrFI | CCNGG | 8 cut(s) | 107, 117, 153, 349, 363, 384, 476, 531 |
| BmsI | GCATC | 1 cut(s) | 438 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 476 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaHI | GRCGYC | 1 cut(s) | 489 |
| BsaJI | CCNNGG | 4 cut(s) | 105, 115, 383, 530 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 151, 187, 256, 368, 439 |
| Bse118I | RCCGGY | 4 cut(s) | 368, 388, 416, 568 |
| Bse1I | ACTGG | 1 cut(s) | 192 |
| BseBI | CCWGG | 3 cut(s) | 349, 384, 531 |
| BseDI | CCNNGG | 4 cut(s) | 105, 115, 383, 530 |
| BseGI | GGATG | 3 cut(s) | 43, 51, 453 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 151, 187, 256, 368, 439 |
| BseMII | CTCAG | 1 cut(s) | 518 |
| BseNI | ACTGG | 1 cut(s) | 192 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseSI | GKGCMC | 2 cut(s) | 94, 537 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 532 |
| BseYI | CCCAGC | 3 cut(s) | 70, 319, 493 |
| BshFI | GGCC | 6 cut(s) | 50, 110, 156, 308, 388, 535 |
| BshNI | GGYRCC | 2 cut(s) | 365, 379 |
| BsiHKAI | GWGCWC | 1 cut(s) | 94 |
| BslFI | GGGAC | 1 cut(s) | 476 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 151, 187, 256, 368, 439 |
| BsmFI | GGGAC | 1 cut(s) | 476 |
| BsnI | GGCC | 6 cut(s) | 50, 110, 156, 308, 388, 535 |
| Bsp120I | GGGCCC | 1 cut(s) | 533 |
| Bsp1286I | GDGCHC | 2 cut(s) | 94, 537 |
| Bsp143I | GATC | 3 cut(s) | 160, 193, 471 |
| BspANI | GGCC | 6 cut(s) | 50, 110, 156, 308, 388, 535 |
| BspCNI | CTCAG | 1 cut(s) | 517 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 3 cut(s) | 367, 381, 535 |
| BspMAI | CTGCAG | 1 cut(s) | 558 |
| BspPI | GGATC | 2 cut(s) | 201, 466 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 365, 379 |
| BsrBI | CCGCTC | 1 cut(s) | 514 |
| BsrFI | RCCGGY | 4 cut(s) | 368, 388, 416, 568 |
| BsrI | ACTGG | 1 cut(s) | 192 |
| BssAI | RCCGGY | 4 cut(s) | 368, 388, 416, 568 |
| BssECI | CCNNGG | 4 cut(s) | 105, 115, 383, 530 |
| BssMI | GATC | 3 cut(s) | 160, 193, 471 |
| BssNI | GRCGYC | 1 cut(s) | 489 |
| Bst2UI | CCWGG | 3 cut(s) | 349, 384, 531 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 1 cut(s) | 489 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 7 cut(s) | 36, 149, 316, 332, 405, 418, 558 |
| BstDEI | CTNAG | 1 cut(s) | 504 |
| BstF5I | GGATG | 3 cut(s) | 43, 51, 453 |
| BstH2I | RGCGCY | 1 cut(s) | 500 |
| BstHHI | GCGC | 3 cut(s) | 231, 403, 499 |
| BstKTI | GATC | 3 cut(s) | 163, 196, 474 |
| BstMBI | GATC | 3 cut(s) | 160, 193, 471 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 144, 153, 320, 413 |
| BstNI | CCWGG | 3 cut(s) | 349, 384, 531 |
| BstNSI | RCATGY | 1 cut(s) | 279 |
| BstSCI | CCNGG | 8 cut(s) | 105, 115, 151, 347, 361, 382, 474, 529 |
| BstSFI | CTRYAG | 1 cut(s) | 554 |
| BstSLI | GKGCMC | 2 cut(s) | 94, 537 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 532 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 6 cut(s) | 50, 110, 156, 308, 388, 535 |
| BtsCI | GGATG | 3 cut(s) | 43, 51, 453 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 7 cut(s) | 36, 149, 316, 332, 405, 418, 558 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 3 cut(s) | 231, 403, 499 |
| Cfr10I | RCCGGY | 4 cut(s) | 368, 388, 416, 568 |
| Cfr13I | GGNCC | 4 cut(s) | 154, 386, 533, 534 |
| CseI | GACGC | 2 cut(s) | 307, 478 |
| Csp6I | GTAC | 2 cut(s) | 175, 380 |
| CviAII | CATG | 2 cut(s) | 271, 276 |
| CviQI | GTAC | 2 cut(s) | 175, 380 |
| DdeI | CTNAG | 1 cut(s) | 504 |
| DpnI | GATC | 3 cut(s) | 162, 195, 473 |
| DpnII | GATC | 3 cut(s) | 160, 193, 471 |
| EaeI | YGGCCR | 2 cut(s) | 48, 108 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 3 cut(s) | 206, 403, 494 |
| Eco24I | GRGCYC | 1 cut(s) | 537 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 264 |
| EcoO109I | RGGNCCY | 1 cut(s) | 534 |
| EcoRII | CCWGG | 3 cut(s) | 347, 382, 529 |
| EcoT38I | GRGCYC | 1 cut(s) | 537 |
| FaeI | CATG | 2 cut(s) | 274, 279 |
| FaiI | YATR | 7 cut(s) | 173, 201, 272, 277, 395, 574, 576 |
| FaqI | GGGAC | 1 cut(s) | 476 |
| FatI | CATG | 2 cut(s) | 270, 275 |
| FauI | CCCGC | 3 cut(s) | 8, 156, 323 |
| FauNDI | CATATG | 1 cut(s) | 574 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 4 cut(s) | 139, 142, 145, 546 |
| FokI | GGATG | 3 cut(s) | 30, 38, 460 |
| FriOI | GRGCYC | 1 cut(s) | 537 |
| Fsp4HI | GCNGC | 4 cut(s) | 139, 142, 145, 546 |
| FspBI | CTAG | 2 cut(s) | 182, 458 |
| GlaI | GCGC | 3 cut(s) | 230, 402, 498 |
| GluI | GCNGC | 4 cut(s) | 139, 142, 145, 546 |
| GsaI | CCCAGC | 3 cut(s) | 74, 323, 497 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 500 |
| HaeIII | GGCC | 6 cut(s) | 50, 110, 156, 308, 388, 535 |
| HgaI | GACGC | 2 cut(s) | 307, 478 |
| HhaI | GCGC | 3 cut(s) | 231, 403, 499 |
| Hin1I | GRCGYC | 1 cut(s) | 489 |
| Hin1II | CATG | 2 cut(s) | 274, 279 |
| Hin6I | GCGC | 3 cut(s) | 229, 401, 497 |
| HinP1I | GCGC | 3 cut(s) | 229, 401, 497 |
| HincII | GTYRAC | 1 cut(s) | 359 |
| HindII | GTYRAC | 1 cut(s) | 359 |
| HinfI | GANTC | 3 cut(s) | 410, 482, 508 |
| HphI | GGTGA | 2 cut(s) | 134, 532 |
| Hpy166II | GTNNAC | 2 cut(s) | 92, 359 |
| Hpy188I | TCNGA | 4 cut(s) | 67, 507, 527, 566 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 2 cut(s) | 92, 359 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 1 cut(s) | 547 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 3 cut(s) | 88, 217, 340 |
| HpyCH4V | TGCA | 5 cut(s) | 92, 451, 548, 556, 578 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 144, 153, 320, 413 |
| HpyF3I | CTNAG | 1 cut(s) | 504 |
| HpySE526I | ACGT | 3 cut(s) | 88, 217, 340 |
| Hsp92I | GRCGYC | 1 cut(s) | 489 |
| Hsp92II | CATG | 2 cut(s) | 274, 279 |
| HspAI | GCGC | 3 cut(s) | 229, 401, 497 |
| KpnI | GGTACC | 1 cut(s) | 383 |
| KroI | GCCGGC | 1 cut(s) | 416 |
| KroNI | GCCGGC | 1 cut(s) | 418 |
| Kzo9I | GATC | 3 cut(s) | 160, 193, 471 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 532 |
| LweI | GCATC | 1 cut(s) | 438 |
| MaeI | CTAG | 2 cut(s) | 182, 458 |
| MaeII | ACGT | 3 cut(s) | 88, 217, 340 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 3 cut(s) | 162, 195, 473 |
| MbiI | CCGCTC | 1 cut(s) | 514 |
| MboI | GATC | 3 cut(s) | 160, 193, 471 |
| MboII | GAAGA | 2 cut(s) | 41, 307 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 2 cut(s) | 94, 537 |
| MlyI | GAGTC | 1 cut(s) | 491 |
| MmeI | TCCRAC | 2 cut(s) | 90, 420 |
| MnlI | CCTC | 8 cut(s) | 73, 118, 214, 217, 277, 278, 298, 459 |
| MreI | CGCCGGCG | 1 cut(s) | 416 |
| MroNI | GCCGGC | 1 cut(s) | 416 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 545 |
| MspR9I | CCNGG | 8 cut(s) | 107, 117, 153, 349, 363, 384, 476, 531 |
| MvaI | CCWGG | 3 cut(s) | 349, 384, 531 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 144, 153, 320, 413 |
| NaeI | GCCGGC | 1 cut(s) | 418 |
| NciI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 476 |
| NdeI | CATATG | 1 cut(s) | 574 |
| NdeII | GATC | 3 cut(s) | 160, 193, 471 |
| NgoMIV | GCCGGC | 1 cut(s) | 416 |
| NlaIII | CATG | 2 cut(s) | 274, 279 |
| NlaIV | GGNNCC | 3 cut(s) | 367, 381, 535 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| NspI | RCATGY | 1 cut(s) | 279 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciI | ACATGT | 1 cut(s) | 275 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 174 |
| PdiI | GCCGGC | 1 cut(s) | 418 |
| PfeI | GAWTC | 2 cut(s) | 410, 508 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 439 |
| PkrI | GCNGC | 4 cut(s) | 140, 143, 146, 547 |
| PleI | GAGTC | 1 cut(s) | 490 |
| PpsI | GAGTC | 1 cut(s) | 490 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| PscI | ACATGT | 1 cut(s) | 275 |
| Psp6I | CCWGG | 3 cut(s) | 347, 382, 529 |
| PspFI | CCCAGC | 3 cut(s) | 70, 319, 493 |
| PspGI | CCWGG | 3 cut(s) | 347, 382, 529 |
| PspN4I | GGNNCC | 3 cut(s) | 367, 381, 535 |
| PspOMI | GGGCCC | 1 cut(s) | 533 |
| PspPI | GGNCC | 4 cut(s) | 154, 386, 533, 534 |
| PstI | CTGCAG | 1 cut(s) | 558 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 2 cut(s) | 176, 381 |
| RsaNI | GTAC | 2 cut(s) | 175, 380 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 4 cut(s) | 139, 142, 145, 546 |
| Sau3AI | GATC | 3 cut(s) | 160, 193, 471 |
| Sau96I | GGNCC | 4 cut(s) | 154, 386, 533, 534 |
| SbfI | CCTGCAGG | 1 cut(s) | 558 |
| SchI | GAGTC | 1 cut(s) | 491 |
| ScrFI | CCNGG | 8 cut(s) | 107, 117, 153, 349, 363, 384, 476, 531 |
| SdaI | CCTGCAGG | 1 cut(s) | 558 |
| SduI | GDGCHC | 2 cut(s) | 94, 537 |
| SetI | ASST | 7 cut(s) | 91, 183, 220, 261, 343, 470, 532 |
| SfaNI | GCATC | 1 cut(s) | 438 |
| SfcI | CTRYAG | 1 cut(s) | 554 |
| SgrAI | CRCCGGYG | 1 cut(s) | 416 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 558 |
| SspMI | CTAG | 2 cut(s) | 182, 458 |
| StyD4I | CCNGG | 8 cut(s) | 105, 115, 151, 347, 361, 382, 474, 529 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 3 cut(s) | 91, 220, 343 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TfiI | GAWTC | 2 cut(s) | 410, 508 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 545 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 439 |
| VneI | GTGCAC | 1 cut(s) | 90 |
| XceI | RCATGY | 1 cut(s) | 279 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XspI | CTAG | 2 cut(s) | 182, 458 |