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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 25, 310 |
| Acc36I | ACCTGC | 2 cut(s) | 25, 310 |
| Acc65I | GGTACC | 2 cut(s) | 442, 469 |
| AccB1I | GGYRCC | 2 cut(s) | 442, 469 |
| AccBSI | CCGCTC | 1 cut(s) | 567 |
| AccI | GTMKAC | 1 cut(s) | 421 |
| AccII | CGCG | 2 cut(s) | 108, 282 |
| AciI | CCGC | 9 cut(s) | 106, 167, 185, 231, 473, 542, 565, 618, 653 |
| AcoI | YGGCCR | 2 cut(s) | 194, 324 |
| AfaI | GTAC | 2 cut(s) | 444, 471 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 265, 319, 431 |
| AgeI | ACCGGT | 2 cut(s) | 214, 431 |
| AgsI | TTSAA | 2 cut(s) | 118, 355 |
| AjnI | CCWGG | 3 cut(s) | 196, 598, 626 |
| AluBI | AGCT | 3 cut(s) | 163, 244, 609 |
| AluI | AGCT | 3 cut(s) | 163, 244, 609 |
| Alw21I | GWGCWC | 1 cut(s) | 410 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 609 |
| AoxI | GGCC | 4 cut(s) | 194, 309, 324, 502 |
| ApaI | GGGCCC | 1 cut(s) | 506 |
| ApeKI | GCWGC | 2 cut(s) | 182, 640 |
| AsiGI | ACCGGT | 2 cut(s) | 214, 431 |
| Asp718I | GGTACC | 2 cut(s) | 442, 469 |
| AspLEI | GCGC | 4 cut(s) | 110, 282, 364, 388 |
| AspS9I | GGNCC | 5 cut(s) | 40, 223, 310, 502, 503 |
| AsuNHI | GCTAGC | 1 cut(s) | 244 |
| AvaII | GGWCC | 2 cut(s) | 40, 223 |
| AxyI | CCTNAGG | 1 cut(s) | 147 |
| BaeGI | GKGCMC | 1 cut(s) | 506 |
| BalI | TGGCCA | 2 cut(s) | 196, 326 |
| BanI | GGYRCC | 2 cut(s) | 442, 469 |
| BanII | GRGCYC | 2 cut(s) | 506, 638 |
| Bbv12I | GWGCWC | 1 cut(s) | 410 |
| BbvI | GCAGC | 2 cut(s) | 194, 627 |
| BccI | CCATC | 2 cut(s) | 66, 71 |
| BceAI | ACGGC | 1 cut(s) | 569 |
| BciT130I | CCWGG | 3 cut(s) | 198, 600, 628 |
| BfaI | CTAG | 3 cut(s) | 135, 245, 582 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BfuAI | ACCTGC | 2 cut(s) | 25, 310 |
| BisI | GCNGC | 3 cut(s) | 183, 231, 641 |
| BlsI | GCNGC | 3 cut(s) | 184, 232, 642 |
| Bme1390I | CCNGG | 3 cut(s) | 198, 600, 628 |
| Bme18I | GGWCC | 2 cut(s) | 40, 223 |
| BmgT120I | GGNCC | 5 cut(s) | 40, 223, 310, 502, 503 |
| BmiI | GGNNCC | 4 cut(s) | 144, 444, 471, 504 |
| BmrFI | CCNGG | 3 cut(s) | 198, 600, 628 |
| BmtI | GCTAGC | 1 cut(s) | 248 |
| BpmI | CTGGAG | 2 cut(s) | 386, 610 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 598 |
| BsaWI | WCCGGW | 2 cut(s) | 214, 431 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 265, 319, 431 |
| Bse118I | RCCGGY | 3 cut(s) | 124, 214, 431 |
| Bse21I | CCTNAGG | 1 cut(s) | 147 |
| BseBI | CCWGG | 3 cut(s) | 198, 600, 628 |
| BseDI | CCNNGG | 2 cut(s) | 72, 598 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 265, 319, 431 |
| BseMII | CTCAG | 1 cut(s) | 161 |
| BseSI | GKGCMC | 1 cut(s) | 506 |
| BseXI | GCAGC | 2 cut(s) | 194, 627 |
| BseYI | CCCAGC | 1 cut(s) | 159 |
| BsgI | GTGCAG | 1 cut(s) | 239 |
| Bsh1236I | CGCG | 2 cut(s) | 108, 282 |
| BshFI | GGCC | 4 cut(s) | 196, 311, 326, 504 |
| BshNI | GGYRCC | 2 cut(s) | 442, 469 |
| BshTI | ACCGGT | 2 cut(s) | 214, 431 |
| BsiHKAI | GWGCWC | 1 cut(s) | 410 |
| BsiSI | CCGG | 3 cut(s) | 125, 215, 432 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 265, 319, 431 |
| BsnI | GGCC | 4 cut(s) | 196, 311, 326, 504 |
| Bsp120I | GGGCCC | 1 cut(s) | 502 |
| Bsp1286I | GDGCHC | 3 cut(s) | 410, 506, 638 |
| BspACI | CCGC | 9 cut(s) | 106, 167, 185, 231, 473, 542, 565, 618, 653 |
| BspANI | GGCC | 4 cut(s) | 196, 311, 326, 504 |
| BspCNI | CTCAG | 1 cut(s) | 160 |
| BspFNI | CGCG | 2 cut(s) | 108, 282 |
| BspLI | GGNNCC | 4 cut(s) | 144, 444, 471, 504 |
| BspMI | ACCTGC | 2 cut(s) | 25, 310 |
| BspOI | GCTAGC | 1 cut(s) | 248 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 442, 469 |
| BsrBI | CCGCTC | 1 cut(s) | 567 |
| BsrFI | RCCGGY | 3 cut(s) | 124, 214, 431 |
| BssAI | RCCGGY | 3 cut(s) | 124, 214, 431 |
| BssECI | CCNNGG | 2 cut(s) | 72, 598 |
| Bst2UI | CCWGG | 3 cut(s) | 198, 600, 628 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 94 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 119 |
| BstC8I | GCNNGC | 5 cut(s) | 246, 284, 395, 611, 638 |
| BstDEI | CTNAG | 1 cut(s) | 147 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 2 cut(s) | 108, 282 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 4 cut(s) | 110, 282, 364, 388 |
| BstNI | CCWGG | 3 cut(s) | 198, 600, 628 |
| BstSCI | CCNGG | 3 cut(s) | 196, 598, 626 |
| BstSLI | GKGCMC | 1 cut(s) | 506 |
| BstUI | CGCG | 2 cut(s) | 108, 282 |
| BstV1I | GCAGC | 2 cut(s) | 194, 627 |
| Bsu36I | CCTNAGG | 1 cut(s) | 147 |
| BsuRI | GGCC | 4 cut(s) | 196, 311, 326, 504 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BveI | ACCTGC | 2 cut(s) | 25, 310 |
| Cac8I | GCNNGC | 5 cut(s) | 246, 284, 395, 611, 638 |
| CaiI | CAGNNNCTG | 1 cut(s) | 609 |
| CfoI | GCGC | 4 cut(s) | 110, 282, 364, 388 |
| Cfr10I | RCCGGY | 3 cut(s) | 124, 214, 431 |
| Cfr13I | GGNCC | 5 cut(s) | 40, 223, 310, 502, 503 |
| Csp6I | GTAC | 2 cut(s) | 443, 470 |
| CspAI | ACCGGT | 2 cut(s) | 214, 431 |
| CviAII | CATG | 2 cut(s) | 139, 287 |
| CviQI | GTAC | 2 cut(s) | 443, 470 |
| DdeI | CTNAG | 1 cut(s) | 147 |
| EaeI | YGGCCR | 2 cut(s) | 194, 324 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 119 |
| EarI | CTCTTC | 2 cut(s) | 24, 119 |
| Eco24I | GRGCYC | 2 cut(s) | 506, 638 |
| Eco47I | GGWCC | 2 cut(s) | 40, 223 |
| Eco81I | CCTNAGG | 1 cut(s) | 147 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| EcoRII | CCWGG | 3 cut(s) | 196, 598, 626 |
| EcoT38I | GRGCYC | 2 cut(s) | 506, 638 |
| FaeI | CATG | 2 cut(s) | 142, 290 |
| FaiI | YATR | 6 cut(s) | 140, 236, 240, 288, 449, 458 |
| FatI | CATG | 2 cut(s) | 138, 286 |
| FauI | CCCGC | 1 cut(s) | 174 |
| FblI | GTMKAC | 1 cut(s) | 421 |
| Fnu4HI | GCNGC | 3 cut(s) | 183, 231, 641 |
| FokI | GGATG | 1 cut(s) | 45 |
| FriOI | GRGCYC | 2 cut(s) | 506, 638 |
| Fsp4HI | GCNGC | 3 cut(s) | 183, 231, 641 |
| FspBI | CTAG | 3 cut(s) | 135, 245, 582 |
| GlaI | GCGC | 4 cut(s) | 109, 281, 363, 387 |
| GluI | GCNGC | 3 cut(s) | 183, 231, 641 |
| GsaI | CCCAGC | 1 cut(s) | 163 |
| GsuI | CTGGAG | 2 cut(s) | 386, 610 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 4 cut(s) | 196, 311, 326, 504 |
| HapII | CCGG | 3 cut(s) | 125, 215, 432 |
| HhaI | GCGC | 4 cut(s) | 110, 282, 364, 388 |
| Hin1II | CATG | 2 cut(s) | 142, 290 |
| Hin6I | GCGC | 4 cut(s) | 108, 280, 362, 386 |
| HinP1I | GCGC | 4 cut(s) | 108, 280, 362, 386 |
| HincII | GTYRAC | 4 cut(s) | 97, 103, 130, 422 |
| HindII | GTYRAC | 4 cut(s) | 97, 103, 130, 422 |
| HinfI | GANTC | 3 cut(s) | 172, 545, 621 |
| HpaI | GTTAAC | 1 cut(s) | 97 |
| HpaII | CCGG | 3 cut(s) | 125, 215, 432 |
| Hpy166II | GTNNAC | 5 cut(s) | 40, 97, 103, 130, 422 |
| Hpy188III | TCNNGA | 1 cut(s) | 339 |
| Hpy8I | GTNNAC | 5 cut(s) | 40, 97, 103, 130, 422 |
| HpyAV | CCTTC | 1 cut(s) | 352 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 94 |
| HpyCH4IV | ACGT | 1 cut(s) | 99 |
| HpyCH4V | TGCA | 1 cut(s) | 220 |
| HpyF3I | CTNAG | 1 cut(s) | 147 |
| HpySE526I | ACGT | 1 cut(s) | 99 |
| Hsp92II | CATG | 2 cut(s) | 142, 290 |
| HspAI | GCGC | 4 cut(s) | 108, 280, 362, 386 |
| KpnI | GGTACC | 2 cut(s) | 446, 473 |
| KspAI | GTTAAC | 1 cut(s) | 97 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 3 cut(s) | 168, 405, 532 |
| Lsp1109I | GCAGC | 2 cut(s) | 194, 627 |
| MaeI | CTAG | 3 cut(s) | 135, 245, 582 |
| MaeII | ACGT | 1 cut(s) | 99 |
| MaeIII | GTNAC | 3 cut(s) | 151, 399, 546 |
| MbiI | CCGCTC | 1 cut(s) | 567 |
| MboII | GAAGA | 3 cut(s) | 41, 106, 367 |
| MhlI | GDGCHC | 3 cut(s) | 410, 506, 638 |
| MlsI | TGGCCA | 2 cut(s) | 196, 326 |
| MluCI | AATT | 2 cut(s) | 350, 585 |
| MluNI | TGGCCA | 2 cut(s) | 196, 326 |
| MlyI | GAGTC | 2 cut(s) | 166, 554 |
| MmeI | TCCRAC | 1 cut(s) | 510 |
| MnlI | CCTC | 8 cut(s) | 156, 181, 236, 269, 301, 361, 519, 583 |
| Mox20I | TGGCCA | 2 cut(s) | 196, 326 |
| MscI | TGGCCA | 2 cut(s) | 196, 326 |
| MseI | TTAA | 1 cut(s) | 96 |
| MslI | CAYNNNNRTG | 2 cut(s) | 137, 332 |
| Msp20I | TGGCCA | 2 cut(s) | 196, 326 |
| MspA1I | CMGCKG | 4 cut(s) | 169, 185, 475, 618 |
| MspI | CCGG | 3 cut(s) | 125, 215, 432 |
| MspR9I | CCNGG | 3 cut(s) | 198, 600, 628 |
| MvaI | CCWGG | 3 cut(s) | 198, 600, 628 |
| MvnI | CGCG | 2 cut(s) | 108, 282 |
| NheI | GCTAGC | 1 cut(s) | 244 |
| NlaIII | CATG | 2 cut(s) | 142, 290 |
| NlaIV | GGNNCC | 4 cut(s) | 144, 444, 471, 504 |
| NmeAIII | GCCGAG | 1 cut(s) | 548 |
| NmuCI | GTSAC | 3 cut(s) | 151, 399, 546 |
| PaqCI | CACCTGC | 2 cut(s) | 25, 310 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 621 |
| PinAI | ACCGGT | 2 cut(s) | 214, 431 |
| PkrI | GCNGC | 3 cut(s) | 184, 232, 642 |
| PleI | GAGTC | 2 cut(s) | 166, 553 |
| PpsI | GAGTC | 2 cut(s) | 166, 553 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| Psp6I | CCWGG | 3 cut(s) | 196, 598, 626 |
| PspFI | CCCAGC | 1 cut(s) | 159 |
| PspGI | CCWGG | 3 cut(s) | 196, 598, 626 |
| PspN4I | GGNNCC | 4 cut(s) | 144, 444, 471, 504 |
| PspOMI | GGGCCC | 1 cut(s) | 502 |
| PspPI | GGNCC | 5 cut(s) | 40, 223, 310, 502, 503 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| PstNI | CAGNNNCTG | 1 cut(s) | 609 |
| RsaI | GTAC | 2 cut(s) | 444, 471 |
| RsaNI | GTAC | 2 cut(s) | 443, 470 |
| RseI | CAYNNNNRTG | 2 cut(s) | 137, 332 |
| SalI | GTCGAC | 1 cut(s) | 420 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 96 |
| SatI | GCNGC | 3 cut(s) | 183, 231, 641 |
| Sau96I | GGNCC | 5 cut(s) | 40, 223, 310, 502, 503 |
| SchI | GAGTC | 2 cut(s) | 166, 554 |
| ScrFI | CCNGG | 3 cut(s) | 198, 600, 628 |
| SduI | GDGCHC | 3 cut(s) | 410, 506, 638 |
| SgrAI | CRCCGGYG | 1 cut(s) | 214 |
| SinI | GGWCC | 2 cut(s) | 40, 223 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 137, 332 |
| Sse9I | AATT | 2 cut(s) | 350, 585 |
| SsiI | CCGC | 9 cut(s) | 106, 167, 185, 231, 473, 542, 565, 618, 653 |
| SspMI | CTAG | 3 cut(s) | 135, 245, 582 |
| StyD4I | CCNGG | 3 cut(s) | 196, 598, 626 |
| TaaI | ACNGT | 2 cut(s) | 11, 94 |
| TaiI | ACGT | 1 cut(s) | 102 |
| TaqI | TCGA | 3 cut(s) | 261, 306, 421 |
| TasI | AATT | 2 cut(s) | 350, 585 |
| TauI | GCSGC | 1 cut(s) | 233 |
| TfiI | GAWTC | 1 cut(s) | 621 |
| Tru1I | TTAA | 1 cut(s) | 96 |
| Tru9I | TTAA | 1 cut(s) | 96 |
| TseFI | GTSAC | 3 cut(s) | 151, 399, 546 |
| TseI | GCWGC | 2 cut(s) | 182, 640 |
| Tsp45I | GTSAC | 3 cut(s) | 151, 399, 546 |
| TspGWI | ACGGA | 2 cut(s) | 102, 418 |
| VpaK11BI | GGWCC | 2 cut(s) | 40, 223 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 70, 606 |
| XmiI | GTMKAC | 1 cut(s) | 421 |
| XspI | CTAG | 3 cut(s) | 135, 245, 582 |