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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 394 |
| AccB1I | GGYRCC | 1 cut(s) | 385 |
| AccBSI | CCGCTC | 2 cut(s) | 504, 542 |
| AccII | CGCG | 2 cut(s) | 90, 325 |
| AclWI | GGATC | 2 cut(s) | 201, 562 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AfaI | GTAC | 1 cut(s) | 453 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 283, 368, 551 |
| AjnI | CCWGG | 3 cut(s) | 151, 318, 347 |
| AluBI | AGCT | 4 cut(s) | 181, 289, 427, 460 |
| AluI | AGCT | 4 cut(s) | 181, 289, 427, 460 |
| Alw26I | GTCTC | 2 cut(s) | 207, 502 |
| AlwI | GGATC | 2 cut(s) | 201, 562 |
| Ama87I | CYCGRG | 2 cut(s) | 505, 513 |
| AoxI | GGCC | 4 cut(s) | 154, 240, 246, 261 |
| ApeKI | GCWGC | 1 cut(s) | 141 |
| AspLEI | GCGC | 2 cut(s) | 92, 325 |
| AspS9I | GGNCC | 3 cut(s) | 247, 406, 440 |
| AsuHPI | GGTGA | 3 cut(s) | 91, 134, 491 |
| AsuNHI | GCTAGC | 1 cut(s) | 427 |
| AvaI | CYCGRG | 2 cut(s) | 505, 513 |
| AvaII | GGWCC | 2 cut(s) | 406, 440 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanI | GGYRCC | 1 cut(s) | 385 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BccI | CCATC | 2 cut(s) | 59, 295 |
| BciT130I | CCWGG | 3 cut(s) | 153, 320, 349 |
| BcoDI | GTCTC | 2 cut(s) | 207, 502 |
| BfaI | CTAG | 2 cut(s) | 6, 428 |
| BfuAI | ACCTGC | 1 cut(s) | 394 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 320 |
| BisI | GCNGC | 2 cut(s) | 142, 145 |
| BlsI | GCNGC | 2 cut(s) | 143, 146 |
| Bme1390I | CCNGG | 3 cut(s) | 153, 320, 349 |
| Bme18I | GGWCC | 2 cut(s) | 406, 440 |
| BmeT110I | CYCGRG | 2 cut(s) | 505, 513 |
| BmgT120I | GGNCC | 3 cut(s) | 247, 406, 440 |
| BmiI | GGNNCC | 3 cut(s) | 387, 408, 441 |
| BmrFI | CCNGG | 3 cut(s) | 153, 320, 349 |
| BmrI | ACTGGG | 1 cut(s) | 111 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BmtI | GCTAGC | 1 cut(s) | 431 |
| BmuI | ACTGGG | 1 cut(s) | 111 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| BsaAI | YACGTR | 1 cut(s) | 137 |
| BsaI | GGTCTC | 2 cut(s) | 207, 502 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 243 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 283, 368, 551 |
| Bse118I | RCCGGY | 1 cut(s) | 382 |
| Bse1I | ACTGG | 3 cut(s) | 117, 192, 389 |
| BseBI | CCWGG | 3 cut(s) | 153, 320, 349 |
| BseDI | CCNNGG | 2 cut(s) | 105, 243 |
| BseGI | GGATG | 1 cut(s) | 51 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 283, 368, 551 |
| BseNI | ACTGG | 3 cut(s) | 117, 192, 389 |
| BseRI | GAGGAG | 1 cut(s) | 299 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| BseYI | CCCAGC | 2 cut(s) | 70, 456 |
| BsgI | GTGCAG | 1 cut(s) | 476 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 325 |
| BshFI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| BshNI | GGYRCC | 1 cut(s) | 385 |
| BsiHKCI | CYCGRG | 2 cut(s) | 505, 513 |
| BsiSI | CCGG | 1 cut(s) | 383 |
| BslFI | GGGAC | 3 cut(s) | 259, 392, 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 283, 368, 551 |
| BsmAI | GTCTC | 2 cut(s) | 207, 502 |
| BsmFI | GGGAC | 3 cut(s) | 259, 392, 453 |
| BsnI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| Bso31I | GGTCTC | 2 cut(s) | 207, 502 |
| BsoBI | CYCGRG | 2 cut(s) | 505, 513 |
| Bsp143I | GATC | 3 cut(s) | 40, 193, 554 |
| BspANI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| BspFNI | CGCG | 2 cut(s) | 90, 325 |
| BspLI | GGNNCC | 3 cut(s) | 387, 408, 441 |
| BspMI | ACCTGC | 1 cut(s) | 394 |
| BspOI | GCTAGC | 1 cut(s) | 431 |
| BspPI | GGATC | 2 cut(s) | 201, 562 |
| BspT107I | GGYRCC | 1 cut(s) | 385 |
| BspTNI | GGTCTC | 2 cut(s) | 207, 502 |
| BsrBI | CCGCTC | 2 cut(s) | 504, 542 |
| BsrFI | RCCGGY | 1 cut(s) | 382 |
| BsrI | ACTGG | 3 cut(s) | 117, 192, 389 |
| BssAI | RCCGGY | 1 cut(s) | 382 |
| BssECI | CCNNGG | 2 cut(s) | 105, 243 |
| BssMI | GATC | 3 cut(s) | 40, 193, 554 |
| Bst2UI | CCWGG | 3 cut(s) | 153, 320, 349 |
| Bst4CI | ACNGT | 1 cut(s) | 218 |
| BstBAI | YACGTR | 1 cut(s) | 137 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 149, 183, 429 |
| BstF5I | GGATG | 1 cut(s) | 51 |
| BstFNI | CGCG | 2 cut(s) | 90, 325 |
| BstHHI | GCGC | 2 cut(s) | 92, 325 |
| BstKTI | GATC | 3 cut(s) | 43, 196, 557 |
| BstMAI | GTCTC | 2 cut(s) | 207, 502 |
| BstMBI | GATC | 3 cut(s) | 40, 193, 554 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 71, 87, 153, 320, 331, 409 |
| BstNI | CCWGG | 3 cut(s) | 153, 320, 349 |
| BstSCI | CCNGG | 3 cut(s) | 151, 318, 347 |
| BstUI | CGCG | 2 cut(s) | 90, 325 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| BtsCI | GGATG | 1 cut(s) | 51 |
| BtsI | GCAGTG | 1 cut(s) | 488 |
| BtsIMutI | CAGTG | 3 cut(s) | 124, 185, 488 |
| BveI | ACCTGC | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 149, 183, 429 |
| CfoI | GCGC | 2 cut(s) | 92, 325 |
| Cfr10I | RCCGGY | 1 cut(s) | 382 |
| Cfr13I | GGNCC | 3 cut(s) | 247, 406, 440 |
| CseI | GACGC | 1 cut(s) | 32 |
| Csp6I | GTAC | 1 cut(s) | 452 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 100, 135 |
| CviAII | CATG | 1 cut(s) | 265 |
| CviQI | GTAC | 1 cut(s) | 452 |
| DpnI | GATC | 3 cut(s) | 42, 195, 556 |
| DpnII | GATC | 3 cut(s) | 40, 193, 554 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| EciI | GGCGGA | 2 cut(s) | 37, 206 |
| Eco31I | GGTCTC | 2 cut(s) | 207, 502 |
| Eco47I | GGWCC | 2 cut(s) | 406, 440 |
| Eco88I | CYCGRG | 2 cut(s) | 505, 513 |
| EcoO109I | RGGNCCY | 1 cut(s) | 406 |
| EcoRII | CCWGG | 3 cut(s) | 151, 318, 347 |
| FaeI | CATG | 1 cut(s) | 268 |
| FaiI | YATR | 6 cut(s) | 175, 177, 266, 368, 399, 401 |
| FaqI | GGGAC | 3 cut(s) | 259, 392, 453 |
| FatI | CATG | 1 cut(s) | 264 |
| FauI | CCCGC | 5 cut(s) | 70, 156, 417, 424, 547 |
| FauNDI | CATATG | 1 cut(s) | 399 |
| Fnu4HI | GCNGC | 2 cut(s) | 142, 145 |
| FokI | GGATG | 1 cut(s) | 38 |
| Fsp4HI | GCNGC | 2 cut(s) | 142, 145 |
| FspBI | CTAG | 2 cut(s) | 6, 428 |
| GlaI | GCGC | 2 cut(s) | 91, 324 |
| GluI | GCNGC | 2 cut(s) | 142, 145 |
| GsaI | CCCAGC | 2 cut(s) | 74, 460 |
| HaeIII | GGCC | 4 cut(s) | 156, 242, 248, 263 |
| HapII | CCGG | 1 cut(s) | 383 |
| HgaI | GACGC | 1 cut(s) | 32 |
| HhaI | GCGC | 2 cut(s) | 92, 325 |
| Hin1II | CATG | 1 cut(s) | 268 |
| Hin6I | GCGC | 2 cut(s) | 90, 323 |
| HinP1I | GCGC | 2 cut(s) | 90, 323 |
| HincII | GTYRAC | 1 cut(s) | 112 |
| HindII | GTYRAC | 1 cut(s) | 112 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaII | CCGG | 1 cut(s) | 383 |
| HphI | GGTGA | 3 cut(s) | 91, 134, 491 |
| Hpy166II | GTNNAC | 2 cut(s) | 112, 221 |
| Hpy188III | TCNNGA | 2 cut(s) | 339, 507 |
| Hpy8I | GTNNAC | 2 cut(s) | 112, 221 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 1 cut(s) | 218 |
| HpyCH4IV | ACGT | 1 cut(s) | 136 |
| HpyCH4V | TGCA | 3 cut(s) | 397, 403, 493 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 71, 87, 153, 320, 331, 409 |
| HpySE526I | ACGT | 1 cut(s) | 136 |
| Hsp92II | CATG | 1 cut(s) | 268 |
| HspAI | GCGC | 2 cut(s) | 90, 323 |
| Kzo9I | GATC | 3 cut(s) | 40, 193, 554 |
| LmnI | GCTCC | 3 cut(s) | 286, 371, 547 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| LweI | GCATC | 1 cut(s) | 90 |
| MaeI | CTAG | 2 cut(s) | 6, 428 |
| MaeII | ACGT | 1 cut(s) | 136 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 3 cut(s) | 42, 195, 556 |
| MbiI | CCGCTC | 2 cut(s) | 504, 542 |
| MboI | GATC | 3 cut(s) | 40, 193, 554 |
| MboII | GAAGA | 2 cut(s) | 32, 188 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 1 cut(s) | 558 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MlyI | GAGTC | 1 cut(s) | 491 |
| MmeI | TCCRAC | 1 cut(s) | 447 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 1 cut(s) | 383 |
| MspR9I | CCNGG | 3 cut(s) | 153, 320, 349 |
| MvaI | CCWGG | 3 cut(s) | 153, 320, 349 |
| MvnI | CGCG | 2 cut(s) | 90, 325 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 71, 87, 153, 320, 331, 409 |
| NdeI | CATATG | 1 cut(s) | 399 |
| NdeII | GATC | 3 cut(s) | 40, 193, 554 |
| NheI | GCTAGC | 1 cut(s) | 427 |
| NlaIII | CATG | 1 cut(s) | 268 |
| NlaIV | GGNNCC | 3 cut(s) | 387, 408, 441 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| PaeR7I | CTCGAG | 2 cut(s) | 505, 513 |
| PkrI | GCNGC | 2 cut(s) | 143, 146 |
| PleI | GAGTC | 1 cut(s) | 490 |
| PpsI | GAGTC | 1 cut(s) | 490 |
| Ppu21I | YACGTR | 1 cut(s) | 137 |
| PpuMI | RGGWCCY | 1 cut(s) | 406 |
| Psp5II | RGGWCCY | 1 cut(s) | 406 |
| Psp6I | CCWGG | 3 cut(s) | 151, 318, 347 |
| PspFI | CCCAGC | 2 cut(s) | 70, 456 |
| PspGI | CCWGG | 3 cut(s) | 151, 318, 347 |
| PspN4I | GGNNCC | 3 cut(s) | 387, 408, 441 |
| PspPI | GGNCC | 3 cut(s) | 247, 406, 440 |
| PspPPI | RGGWCCY | 1 cut(s) | 406 |
| PspXI | VCTCGAGB | 1 cut(s) | 513 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 1 cut(s) | 453 |
| RsaNI | GTAC | 1 cut(s) | 452 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SatI | GCNGC | 2 cut(s) | 142, 145 |
| Sau3AI | GATC | 3 cut(s) | 40, 193, 554 |
| Sau96I | GGNCC | 3 cut(s) | 247, 406, 440 |
| SchI | GAGTC | 1 cut(s) | 491 |
| ScrFI | CCNGG | 3 cut(s) | 153, 320, 349 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| Sfr274I | CTCGAG | 2 cut(s) | 505, 513 |
| SinI | GGWCC | 2 cut(s) | 406, 440 |
| SlaI | CTCGAG | 2 cut(s) | 505, 513 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SmlI | CTYRAG | 2 cut(s) | 505, 513 |
| SmoI | CTYRAG | 2 cut(s) | 505, 513 |
| Sse9I | AATT | 1 cut(s) | 558 |
| SspMI | CTAG | 2 cut(s) | 6, 428 |
| StyD4I | CCNGG | 3 cut(s) | 151, 318, 347 |
| TaaI | ACNGT | 1 cut(s) | 218 |
| TaiI | ACGT | 1 cut(s) | 139 |
| TaqI | TCGA | 2 cut(s) | 506, 514 |
| TaqII | GACCGA | 1 cut(s) | 97 |
| TasI | AATT | 1 cut(s) | 558 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TscAI | CASTG | 3 cut(s) | 124, 192, 495 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 1 cut(s) | 141 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspRI | CASTG | 3 cut(s) | 124, 192, 495 |
| VpaK11BI | GGWCC | 2 cut(s) | 406, 440 |
| XhoI | CTCGAG | 2 cut(s) | 505, 513 |
| XspI | CTAG | 2 cut(s) | 6, 428 |