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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 113, 140, 211, 357 |
| AclWI | GGATC | 1 cut(s) | 358 |
| AcuI | CTGAAG | 1 cut(s) | 41 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 117, 210, 211, 271 |
| AgeI | ACCGGT | 1 cut(s) | 248 |
| AgsI | TTSAA | 1 cut(s) | 447 |
| AjnI | CCWGG | 6 cut(s) | 116, 206, 234, 270, 338, 381 |
| AluBI | AGCT | 3 cut(s) | 19, 217, 289 |
| AluI | AGCT | 3 cut(s) | 19, 217, 289 |
| Alw21I | GWGCWC | 2 cut(s) | 77, 313 |
| AlwI | GGATC | 1 cut(s) | 358 |
| AoxI | GGCC | 4 cut(s) | 100, 219, 242, 341 |
| ApaI | GGGCCC | 1 cut(s) | 246 |
| AsiGI | ACCGGT | 1 cut(s) | 248 |
| AspLEI | GCGC | 2 cut(s) | 55, 388 |
| AspS9I | GGNCC | 4 cut(s) | 100, 220, 242, 243 |
| AsuHPI | GGTGA | 3 cut(s) | 263, 346, 447 |
| BaeGI | GKGCMC | 1 cut(s) | 246 |
| BanII | GRGCYC | 1 cut(s) | 246 |
| Bbv12I | GWGCWC | 2 cut(s) | 77, 313 |
| BccI | CCATC | 2 cut(s) | 91, 383 |
| BceAI | ACGGC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| BfaI | CTAG | 2 cut(s) | 78, 290 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 50 |
| Bme1390I | CCNGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| BmgT120I | GGNCC | 4 cut(s) | 100, 220, 242, 243 |
| BmiI | GGNNCC | 2 cut(s) | 230, 244 |
| BmrFI | CCNGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| BmsI | GCATC | 1 cut(s) | 270 |
| BpmI | CTGGAG | 1 cut(s) | 218 |
| BsaJI | CCNNGG | 2 cut(s) | 116, 206 |
| BsaWI | WCCGGW | 1 cut(s) | 248 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 128, 158 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 117, 210, 211, 271 |
| Bse118I | RCCGGY | 1 cut(s) | 248 |
| BseBI | CCWGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| BseDI | CCNNGG | 2 cut(s) | 116, 206 |
| BseGI | GGATG | 2 cut(s) | 102, 285 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 117, 210, 211, 271 |
| BseMII | CTCAG | 1 cut(s) | 177 |
| BseSI | GKGCMC | 1 cut(s) | 246 |
| BshFI | GGCC | 4 cut(s) | 102, 221, 244, 343 |
| BshTI | ACCGGT | 1 cut(s) | 248 |
| BsiHKAI | GWGCWC | 2 cut(s) | 77, 313 |
| BsiSI | CCGG | 1 cut(s) | 249 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 117, 210, 211, 271 |
| BsnI | GGCC | 4 cut(s) | 102, 221, 244, 343 |
| Bsp120I | GGGCCC | 1 cut(s) | 242 |
| Bsp1286I | GDGCHC | 3 cut(s) | 77, 246, 313 |
| Bsp143I | GATC | 2 cut(s) | 171, 363 |
| BspACI | CCGC | 4 cut(s) | 113, 140, 211, 357 |
| BspANI | GGCC | 4 cut(s) | 102, 221, 244, 343 |
| BspCNI | CTCAG | 1 cut(s) | 178 |
| BspLI | GGNNCC | 2 cut(s) | 230, 244 |
| BspPI | GGATC | 1 cut(s) | 358 |
| BsrFI | RCCGGY | 1 cut(s) | 248 |
| BssAI | RCCGGY | 1 cut(s) | 248 |
| BssECI | CCNNGG | 2 cut(s) | 116, 206 |
| BssMI | GATC | 2 cut(s) | 171, 363 |
| Bst2UI | CCWGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| Bst4CI | ACNGT | 1 cut(s) | 410 |
| BstC8I | GCNNGC | 4 cut(s) | 62, 219, 372, 390 |
| BstDEI | CTNAG | 1 cut(s) | 186 |
| BstF5I | GGATG | 2 cut(s) | 102, 285 |
| BstHHI | GCGC | 2 cut(s) | 55, 388 |
| BstKTI | GATC | 2 cut(s) | 174, 366 |
| BstMBI | GATC | 2 cut(s) | 171, 363 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 50, 61 |
| BstNI | CCWGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| BstNSI | RCATGY | 1 cut(s) | 374 |
| BstSCI | CCNGG | 6 cut(s) | 116, 206, 234, 270, 338, 381 |
| BstSLI | GKGCMC | 1 cut(s) | 246 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 382 |
| BsuRI | GGCC | 4 cut(s) | 102, 221, 244, 343 |
| BtsCI | GGATG | 2 cut(s) | 102, 285 |
| Cac8I | GCNNGC | 4 cut(s) | 62, 219, 372, 390 |
| CfoI | GCGC | 2 cut(s) | 55, 388 |
| Cfr10I | RCCGGY | 1 cut(s) | 248 |
| Cfr13I | GGNCC | 4 cut(s) | 100, 220, 242, 243 |
| CseI | GACGC | 3 cut(s) | 263, 324, 487 |
| CspAI | ACCGGT | 1 cut(s) | 248 |
| CviAII | CATG | 3 cut(s) | 361, 371, 415 |
| DdeI | CTNAG | 1 cut(s) | 186 |
| DpnI | GATC | 2 cut(s) | 173, 365 |
| DpnII | GATC | 2 cut(s) | 171, 363 |
| EciI | GGCGGA | 1 cut(s) | 155 |
| Eco24I | GRGCYC | 1 cut(s) | 246 |
| Eco57I | CTGAAG | 1 cut(s) | 41 |
| EcoO109I | RGGNCCY | 1 cut(s) | 242 |
| EcoRII | CCWGG | 6 cut(s) | 116, 206, 234, 270, 338, 381 |
| EcoT38I | GRGCYC | 1 cut(s) | 246 |
| FaeI | CATG | 3 cut(s) | 364, 374, 418 |
| FaiI | YATR | 5 cut(s) | 362, 372, 416, 434, 436 |
| FatI | CATG | 3 cut(s) | 360, 370, 414 |
| FauI | CCCGC | 2 cut(s) | 120, 204 |
| FauNDI | CATATG | 1 cut(s) | 434 |
| FokI | GGATG | 2 cut(s) | 109, 292 |
| FriOI | GRGCYC | 1 cut(s) | 246 |
| FspBI | CTAG | 2 cut(s) | 78, 290 |
| GlaI | GCGC | 2 cut(s) | 54, 387 |
| GsuI | CTGGAG | 1 cut(s) | 218 |
| HaeIII | GGCC | 4 cut(s) | 102, 221, 244, 343 |
| HapII | CCGG | 1 cut(s) | 249 |
| HgaI | GACGC | 3 cut(s) | 263, 324, 487 |
| HhaI | GCGC | 2 cut(s) | 55, 388 |
| Hin1II | CATG | 3 cut(s) | 364, 374, 418 |
| Hin6I | GCGC | 2 cut(s) | 53, 386 |
| HinP1I | GCGC | 2 cut(s) | 53, 386 |
| HinfI | GANTC | 2 cut(s) | 143, 470 |
| HpaII | CCGG | 1 cut(s) | 249 |
| HphI | GGTGA | 3 cut(s) | 263, 346, 447 |
| Hpy188I | TCNGA | 1 cut(s) | 308 |
| Hpy188III | TCNNGA | 1 cut(s) | 447 |
| HpyAV | CCTTC | 2 cut(s) | 19, 453 |
| HpyCH4III | ACNGT | 1 cut(s) | 410 |
| HpyCH4V | TGCA | 2 cut(s) | 283, 370 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 50, 61 |
| HpyF3I | CTNAG | 1 cut(s) | 186 |
| Hsp92II | CATG | 3 cut(s) | 364, 374, 418 |
| HspAI | GCGC | 2 cut(s) | 53, 386 |
| Kzo9I | GATC | 2 cut(s) | 171, 363 |
| LmnI | GCTCC | 3 cut(s) | 72, 130, 214 |
| LweI | GCATC | 1 cut(s) | 270 |
| MaeI | CTAG | 2 cut(s) | 78, 290 |
| MaeIII | GTNAC | 4 cut(s) | 144, 251, 399, 453 |
| MalI | GATC | 2 cut(s) | 173, 365 |
| MboI | GATC | 2 cut(s) | 171, 363 |
| MboII | GAAGA | 1 cut(s) | 34 |
| MhlI | GDGCHC | 3 cut(s) | 77, 246, 313 |
| MlyI | GAGTC | 1 cut(s) | 152 |
| MmeI | TCCRAC | 1 cut(s) | 108 |
| MnlI | CCTC | 6 cut(s) | 117, 130, 181, 219, 242, 496 |
| MslI | CAYNNNNRTG | 2 cut(s) | 359, 408 |
| MspI | CCGG | 1 cut(s) | 249 |
| MspR9I | CCNGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| MvaI | CCWGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 50, 61 |
| NdeI | CATATG | 1 cut(s) | 434 |
| NdeII | GATC | 2 cut(s) | 171, 363 |
| NlaIII | CATG | 3 cut(s) | 364, 374, 418 |
| NlaIV | GGNNCC | 2 cut(s) | 230, 244 |
| NmeAIII | GCCGAG | 1 cut(s) | 146 |
| NmuCI | GTSAC | 4 cut(s) | 144, 251, 399, 453 |
| NspI | RCATGY | 1 cut(s) | 374 |
| PaeI | GCATGC | 1 cut(s) | 374 |
| PfeI | GAWTC | 1 cut(s) | 470 |
| PfoI | TCCNGGA | 1 cut(s) | 270 |
| PinAI | ACCGGT | 1 cut(s) | 248 |
| PleI | GAGTC | 1 cut(s) | 151 |
| PpsI | GAGTC | 1 cut(s) | 151 |
| Psp6I | CCWGG | 6 cut(s) | 116, 206, 234, 270, 338, 381 |
| PspGI | CCWGG | 6 cut(s) | 116, 206, 234, 270, 338, 381 |
| PspN4I | GGNNCC | 2 cut(s) | 230, 244 |
| PspOMI | GGGCCC | 1 cut(s) | 242 |
| PspPI | GGNCC | 4 cut(s) | 100, 220, 242, 243 |
| RseI | CAYNNNNRTG | 2 cut(s) | 359, 408 |
| Sau3AI | GATC | 2 cut(s) | 171, 363 |
| Sau96I | GGNCC | 4 cut(s) | 100, 220, 242, 243 |
| SchI | GAGTC | 1 cut(s) | 152 |
| ScrFI | CCNGG | 6 cut(s) | 118, 208, 236, 272, 340, 383 |
| SduI | GDGCHC | 3 cut(s) | 77, 246, 313 |
| SetI | ASST | 7 cut(s) | 21, 30, 109, 219, 234, 291, 445 |
| SfaNI | GCATC | 1 cut(s) | 270 |
| SgrAI | CRCCGGYG | 1 cut(s) | 248 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 359, 408 |
| SphI | GCATGC | 1 cut(s) | 374 |
| SsiI | CCGC | 4 cut(s) | 113, 140, 211, 357 |
| SspMI | CTAG | 2 cut(s) | 78, 290 |
| StyD4I | CCNGG | 6 cut(s) | 116, 206, 234, 270, 338, 381 |
| TaaI | ACNGT | 1 cut(s) | 410 |
| TaqI | TCGA | 1 cut(s) | 473 |
| TfiI | GAWTC | 1 cut(s) | 470 |
| TseFI | GTSAC | 4 cut(s) | 144, 251, 399, 453 |
| Tsp45I | GTSAC | 4 cut(s) | 144, 251, 399, 453 |
| TspGWI | ACGGA | 1 cut(s) | 85 |
| XceI | RCATGY | 1 cut(s) | 374 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 124, 214, 432 |
| XspI | CTAG | 2 cut(s) | 78, 290 |