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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 721 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1056 |
| Acc16I | TGCGCA | 1 cut(s) | 17 |
| Acc36I | ACCTGC | 1 cut(s) | 721 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 244 |
| AccI | GTMKAC | 3 cut(s) | 6, 616, 832 |
| AccII | CGCG | 5 cut(s) | 209, 224, 693, 814, 853 |
| AclI | AACGTT | 4 cut(s) | 479, 537, 555, 1123 |
| AcoI | YGGCCR | 1 cut(s) | 735 |
| AcsI | RAATTY | 2 cut(s) | 752, 1084 |
| AcuI | CTGAAG | 1 cut(s) | 273 |
| AcyI | GRCGYC | 3 cut(s) | 440, 922, 1094 |
| AfaI | GTAC | 3 cut(s) | 140, 146, 163 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 244, 336, 595, 676, 730, 842, 914 |
| AgeI | ACCGGT | 1 cut(s) | 625 |
| AgsI | TTSAA | 1 cut(s) | 574 |
| AjnI | CCWGG | 2 cut(s) | 630, 962 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 278, 310 |
| AluBI | AGCT | 6 cut(s) | 235, 244, 294, 533, 655, 1145 |
| AluI | AGCT | 6 cut(s) | 235, 244, 294, 533, 655, 1145 |
| Alw21I | GWGCWC | 1 cut(s) | 821 |
| Alw26I | GTCTC | 3 cut(s) | 89, 434, 593 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 244 |
| AoxI | GGCC | 3 cut(s) | 720, 735, 936 |
| ApeKI | GCWGC | 1 cut(s) | 530 |
| ApoI | RAATTY | 2 cut(s) | 752, 1084 |
| AsiGI | ACCGGT | 1 cut(s) | 625 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 479 |
| AspLEI | GCGC | 9 cut(s) | 18, 117, 211, 221, 226, 693, 705, 775, 932 |
| AspS9I | GGNCC | 4 cut(s) | 628, 667, 721, 966 |
| AsuC2I | CCSGG | 3 cut(s) | 182, 609, 909 |
| AsuHPI | GGTGA | 4 cut(s) | 145, 190, 631, 965 |
| AsuNHI | GCTAGC | 1 cut(s) | 655 |
| AvaII | GGWCC | 3 cut(s) | 628, 667, 966 |
| BalI | TGGCCA | 1 cut(s) | 737 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 131, 163 |
| BbsI | GAAGAC | 1 cut(s) | 419 |
| Bbv12I | GWGCWC | 1 cut(s) | 821 |
| BbvI | GCAGC | 1 cut(s) | 542 |
| BccI | CCATC | 1 cut(s) | 589 |
| BceAI | ACGGC | 1 cut(s) | 1055 |
| BciT130I | CCWGG | 2 cut(s) | 632, 964 |
| BcnI | CCSGG | 3 cut(s) | 182, 609, 909 |
| BcoDI | GTCTC | 3 cut(s) | 89, 434, 593 |
| BfaI | CTAG | 2 cut(s) | 656, 873 |
| BfmI | CTRYAG | 2 cut(s) | 358, 987 |
| BfoI | RGCGCY | 2 cut(s) | 776, 933 |
| BfuAI | ACCTGC | 1 cut(s) | 721 |
| BisI | GCNGC | 3 cut(s) | 222, 531, 594 |
| BlsI | GCNGC | 3 cut(s) | 223, 532, 595 |
| Bme1390I | CCNGG | 5 cut(s) | 182, 609, 632, 909, 964 |
| Bme18I | GGWCC | 3 cut(s) | 628, 667, 966 |
| BmgT120I | GGNCC | 4 cut(s) | 628, 667, 721, 966 |
| BmrFI | CCNGG | 5 cut(s) | 182, 609, 632, 909, 964 |
| BmrI | ACTGGG | 1 cut(s) | 539 |
| BmsI | GCATC | 3 cut(s) | 27, 408, 871 |
| BmtI | GCTAGC | 1 cut(s) | 659 |
| BmuI | ACTGGG | 1 cut(s) | 539 |
| BpiI | GAAGAC | 1 cut(s) | 419 |
| BpmI | CTGGAG | 1 cut(s) | 221 |
| BpuMI | CCSGG | 3 cut(s) | 182, 609, 909 |
| BsaHI | GRCGYC | 3 cut(s) | 440, 922, 1094 |
| BsaI | GGTCTC | 2 cut(s) | 434, 593 |
| BsaJI | CCNNGG | 2 cut(s) | 180, 933 |
| BsaWI | WCCGGW | 1 cut(s) | 625 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 278, 308 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 244, 336, 595, 676, 730, 842, 914 |
| Bse118I | RCCGGY | 1 cut(s) | 625 |
| Bse1I | ACTGG | 2 cut(s) | 498, 545 |
| BseBI | CCWGG | 2 cut(s) | 632, 964 |
| BseDI | CCNNGG | 2 cut(s) | 180, 933 |
| BseGI | GGATG | 3 cut(s) | 18, 886, 954 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 244, 336, 595, 676, 730, 842, 914 |
| BseMII | CTCAG | 1 cut(s) | 66 |
| BseNI | ACTGG | 2 cut(s) | 498, 545 |
| BseRI | GAGGAG | 3 cut(s) | 97, 142, 319 |
| BseXI | GCAGC | 1 cut(s) | 542 |
| BseYI | CCCAGC | 2 cut(s) | 244, 926 |
| Bsh1236I | CGCG | 5 cut(s) | 209, 224, 693, 814, 853 |
| BshFI | GGCC | 3 cut(s) | 722, 737, 938 |
| BshTI | ACCGGT | 1 cut(s) | 625 |
| BsiHKAI | GWGCWC | 1 cut(s) | 821 |
| BsiSI | CCGG | 4 cut(s) | 182, 608, 626, 908 |
| BslFI | GGGAC | 3 cut(s) | 262, 862, 909 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 244, 336, 595, 676, 730, 842, 914 |
| BsmAI | GTCTC | 3 cut(s) | 89, 434, 593 |
| BsmFI | GGGAC | 3 cut(s) | 262, 862, 909 |
| BsnI | GGCC | 3 cut(s) | 722, 737, 938 |
| Bso31I | GGTCTC | 2 cut(s) | 434, 593 |
| Bsp1286I | GDGCHC | 1 cut(s) | 821 |
| Bsp143I | GATC | 1 cut(s) | 195 |
| BspANI | GGCC | 3 cut(s) | 722, 737, 938 |
| BspCNI | CTCAG | 1 cut(s) | 65 |
| BspFNI | CGCG | 5 cut(s) | 209, 224, 693, 814, 853 |
| BspHI | TCATGA | 1 cut(s) | 562 |
| BspMI | ACCTGC | 1 cut(s) | 721 |
| BspOI | GCTAGC | 1 cut(s) | 659 |
| BspTNI | GGTCTC | 2 cut(s) | 434, 593 |
| BsrFI | RCCGGY | 1 cut(s) | 625 |
| BsrI | ACTGG | 2 cut(s) | 498, 545 |
| BssAI | RCCGGY | 1 cut(s) | 625 |
| BssECI | CCNNGG | 2 cut(s) | 180, 933 |
| BssMI | GATC | 1 cut(s) | 195 |
| BssNAI | GTATAC | 1 cut(s) | 7 |
| BssNI | GRCGYC | 3 cut(s) | 440, 922, 1094 |
| BssT1I | CCWWGG | 1 cut(s) | 933 |
| Bst1107I | GTATAC | 1 cut(s) | 7 |
| Bst2UI | CCWGG | 2 cut(s) | 632, 964 |
| Bst4CI | ACNGT | 3 cut(s) | 92, 362, 830 |
| Bst6I | CTCTTC | 2 cut(s) | 65, 402 |
| BstACI | GRCGYC | 3 cut(s) | 440, 922, 1094 |
| BstC8I | GCNNGC | 5 cut(s) | 491, 657, 695, 806, 920 |
| BstDEI | CTNAG | 4 cut(s) | 52, 394, 526, 1152 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 334, 674 |
| BstF5I | GGATG | 3 cut(s) | 18, 886, 954 |
| BstFNI | CGCG | 5 cut(s) | 209, 224, 693, 814, 853 |
| BstH2I | RGCGCY | 2 cut(s) | 776, 933 |
| BstHHI | GCGC | 9 cut(s) | 18, 117, 211, 221, 226, 693, 705, 775, 932 |
| BstKTI | GATC | 1 cut(s) | 198 |
| BstMAI | GTCTC | 3 cut(s) | 89, 434, 593 |
| BstMBI | GATC | 1 cut(s) | 195 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 232, 241, 779, 794, 944 |
| BstNI | CCWGG | 2 cut(s) | 632, 964 |
| BstNSI | RCATGY | 1 cut(s) | 1046 |
| BstSCI | CCNGG | 5 cut(s) | 180, 607, 630, 907, 962 |
| BstSFI | CTRYAG | 2 cut(s) | 358, 987 |
| BstUI | CGCG | 5 cut(s) | 209, 224, 693, 814, 853 |
| BstV1I | GCAGC | 1 cut(s) | 542 |
| BstV2I | GAAGAC | 1 cut(s) | 419 |
| BstZ17I | GTATAC | 1 cut(s) | 7 |
| BsuRI | GGCC | 3 cut(s) | 722, 737, 938 |
| BtsCI | GGATG | 3 cut(s) | 18, 886, 954 |
| BtsI | GCAGTG | 1 cut(s) | 11 |
| BtsIMutI | CAGTG | 2 cut(s) | 11, 552 |
| BveI | ACCTGC | 1 cut(s) | 721 |
| Cac8I | GCNNGC | 5 cut(s) | 491, 657, 695, 806, 920 |
| CaiI | CAGNNNCTG | 1 cut(s) | 244 |
| CciI | TCATGA | 1 cut(s) | 562 |
| CfoI | GCGC | 9 cut(s) | 18, 117, 211, 221, 226, 693, 705, 775, 932 |
| Cfr10I | RCCGGY | 1 cut(s) | 625 |
| Cfr13I | GGNCC | 4 cut(s) | 628, 667, 721, 966 |
| CseI | GACGC | 4 cut(s) | 448, 859, 911, 1102 |
| Csp6I | GTAC | 3 cut(s) | 139, 145, 162 |
| CspAI | ACCGGT | 1 cut(s) | 625 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 826, 861 |
| CviAII | CATG | 6 cut(s) | 112, 216, 280, 563, 1043, 1064 |
| CviQI | GTAC | 3 cut(s) | 139, 145, 162 |
| DdeI | CTNAG | 4 cut(s) | 52, 394, 526, 1152 |
| DpnI | GATC | 1 cut(s) | 197 |
| DpnII | GATC | 1 cut(s) | 195 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1056 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1056 |
| EaeI | YGGCCR | 1 cut(s) | 735 |
| Eam1104I | CTCTTC | 2 cut(s) | 65, 402 |
| EarI | CTCTTC | 2 cut(s) | 65, 402 |
| EciI | GGCGGA | 2 cut(s) | 611, 927 |
| Eco130I | CCWWGG | 1 cut(s) | 933 |
| Eco31I | GGTCTC | 2 cut(s) | 434, 593 |
| Eco47I | GGWCC | 3 cut(s) | 628, 667, 966 |
| Eco57I | CTGAAG | 1 cut(s) | 273 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 334, 674 |
| EcoRII | CCWGG | 2 cut(s) | 630, 962 |
| EcoT14I | CCWWGG | 1 cut(s) | 933 |
| ErhI | CCWWGG | 1 cut(s) | 933 |
| FaeI | CATG | 6 cut(s) | 115, 219, 283, 566, 1046, 1067 |
| FaqI | GGGAC | 3 cut(s) | 262, 862, 909 |
| FatI | CATG | 6 cut(s) | 111, 215, 279, 562, 1042, 1063 |
| FblI | GTMKAC | 3 cut(s) | 6, 616, 832 |
| Fnu4HI | GCNGC | 3 cut(s) | 222, 531, 594 |
| FokI | GGATG | 3 cut(s) | 5, 893, 941 |
| Fsp4HI | GCNGC | 3 cut(s) | 222, 531, 594 |
| FspBI | CTAG | 2 cut(s) | 656, 873 |
| FspI | TGCGCA | 1 cut(s) | 17 |
| GlaI | GCGC | 9 cut(s) | 17, 116, 210, 220, 225, 692, 704, 774, 931 |
| GluI | GCNGC | 3 cut(s) | 222, 531, 594 |
| GsaI | CCCAGC | 2 cut(s) | 248, 930 |
| GsuI | CTGGAG | 1 cut(s) | 221 |
| HaeII | RGCGCY | 2 cut(s) | 776, 933 |
| HaeIII | GGCC | 3 cut(s) | 722, 737, 938 |
| HapII | CCGG | 4 cut(s) | 182, 608, 626, 908 |
| HgaI | GACGC | 4 cut(s) | 448, 859, 911, 1102 |
| HhaI | GCGC | 9 cut(s) | 18, 117, 211, 221, 226, 693, 705, 775, 932 |
| Hin1I | GRCGYC | 3 cut(s) | 440, 922, 1094 |
| Hin1II | CATG | 6 cut(s) | 115, 219, 283, 566, 1046, 1067 |
| Hin6I | GCGC | 9 cut(s) | 16, 115, 209, 219, 224, 691, 703, 773, 930 |
| HinP1I | GCGC | 9 cut(s) | 16, 115, 209, 219, 224, 691, 703, 773, 930 |
| HincII | GTYRAC | 3 cut(s) | 373, 541, 833 |
| HindII | GTYRAC | 3 cut(s) | 373, 541, 833 |
| HpaII | CCGG | 4 cut(s) | 182, 608, 626, 908 |
| HphI | GGTGA | 4 cut(s) | 145, 190, 631, 965 |
| Hpy166II | GTNNAC | 6 cut(s) | 7, 373, 541, 617, 833, 1061 |
| Hpy188I | TCNGA | 4 cut(s) | 404, 438, 999, 1049 |
| Hpy188III | TCNNGA | 3 cut(s) | 238, 563, 958 |
| Hpy8I | GTNNAC | 6 cut(s) | 7, 373, 541, 617, 833, 1061 |
| Hpy99I | CGWCG | 1 cut(s) | 761 |
| HpyAV | CCTTC | 3 cut(s) | 453, 706, 1064 |
| HpyCH4III | ACNGT | 3 cut(s) | 92, 362, 830 |
| HpyCH4IV | ACGT | 4 cut(s) | 479, 537, 555, 1123 |
| HpyCH4V | TGCA | 1 cut(s) | 884 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 232, 241, 779, 794, 944 |
| HpyF3I | CTNAG | 4 cut(s) | 52, 394, 526, 1152 |
| HpySE526I | ACGT | 4 cut(s) | 479, 537, 555, 1123 |
| Hsp92I | GRCGYC | 3 cut(s) | 440, 922, 1094 |
| Hsp92II | CATG | 6 cut(s) | 115, 219, 283, 566, 1046, 1067 |
| HspAI | GCGC | 9 cut(s) | 16, 115, 209, 219, 224, 691, 703, 773, 930 |
| Kzo9I | GATC | 1 cut(s) | 195 |
| LmnI | GCTCC | 4 cut(s) | 240, 299, 688, 816 |
| Lsp1109I | GCAGC | 1 cut(s) | 542 |
| LweI | GCATC | 3 cut(s) | 27, 408, 871 |
| MaeI | CTAG | 2 cut(s) | 656, 873 |
| MaeII | ACGT | 4 cut(s) | 479, 537, 555, 1123 |
| MaeIII | GTNAC | 3 cut(s) | 151, 362, 1027 |
| MalI | GATC | 1 cut(s) | 197 |
| MboI | GATC | 1 cut(s) | 195 |
| MboII | GAAGA | 3 cut(s) | 52, 419, 424 |
| MhlI | GDGCHC | 1 cut(s) | 821 |
| MlsI | TGGCCA | 1 cut(s) | 737 |
| MluCI | AATT | 4 cut(s) | 752, 761, 888, 1084 |
| MluNI | TGGCCA | 1 cut(s) | 737 |
| MmeI | TCCRAC | 2 cut(s) | 1072, 1106 |
| Mox20I | TGGCCA | 1 cut(s) | 737 |
| MroXI | GAANNNNTTC | 1 cut(s) | 479 |
| MscI | TGGCCA | 1 cut(s) | 737 |
| MseI | TTAA | 4 cut(s) | 75, 335, 558, 764 |
| MslI | CAYNNNNRTG | 2 cut(s) | 644, 743 |
| Msp20I | TGGCCA | 1 cut(s) | 737 |
| MspA1I | CMGCKG | 1 cut(s) | 464 |
| MspI | CCGG | 4 cut(s) | 182, 608, 626, 908 |
| MspR9I | CCNGG | 5 cut(s) | 182, 609, 632, 909, 964 |
| MteI | GCGCNGCGC | 1 cut(s) | 222 |
| MvaI | CCWGG | 2 cut(s) | 632, 964 |
| MvnI | CGCG | 5 cut(s) | 209, 224, 693, 814, 853 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 232, 241, 779, 794, 944 |
| NciI | CCSGG | 3 cut(s) | 182, 609, 909 |
| NdeII | GATC | 1 cut(s) | 195 |
| NheI | GCTAGC | 1 cut(s) | 655 |
| NlaIII | CATG | 6 cut(s) | 115, 219, 283, 566, 1046, 1067 |
| NmuCI | GTSAC | 2 cut(s) | 151, 1027 |
| NsbI | TGCGCA | 1 cut(s) | 17 |
| NspI | RCATGY | 1 cut(s) | 1046 |
| PagI | TCATGA | 1 cut(s) | 562 |
| PaqCI | CACCTGC | 1 cut(s) | 721 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1045 |
| PdmI | GAANNNNTTC | 1 cut(s) | 479 |
| PflFI | GACNNNGTC | 1 cut(s) | 828 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 244 |
| PfoI | TCCNGGA | 1 cut(s) | 630 |
| PinAI | ACCGGT | 1 cut(s) | 625 |
| PkrI | GCNGC | 3 cut(s) | 223, 532, 595 |
| Psp1406I | AACGTT | 4 cut(s) | 479, 537, 555, 1123 |
| Psp6I | CCWGG | 2 cut(s) | 630, 962 |
| PspFI | CCCAGC | 2 cut(s) | 244, 926 |
| PspGI | CCWGG | 2 cut(s) | 630, 962 |
| PspPI | GGNCC | 4 cut(s) | 628, 667, 721, 966 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 20, 52 |
| PstNI | CAGNNNCTG | 1 cut(s) | 244 |
| PsyI | GACNNNGTC | 1 cut(s) | 828 |
| RsaI | GTAC | 3 cut(s) | 140, 146, 163 |
| RsaNI | GTAC | 3 cut(s) | 139, 145, 162 |
| RseI | CAYNNNNRTG | 2 cut(s) | 644, 743 |
| SalI | GTCGAC | 1 cut(s) | 831 |
| SaqAI | TTAA | 4 cut(s) | 75, 335, 558, 764 |
| SatI | GCNGC | 3 cut(s) | 222, 531, 594 |
| Sau3AI | GATC | 1 cut(s) | 195 |
| Sau96I | GGNCC | 4 cut(s) | 628, 667, 721, 966 |
| ScrFI | CCNGG | 5 cut(s) | 182, 609, 632, 909, 964 |
| SduI | GDGCHC | 1 cut(s) | 821 |
| SfaNI | GCATC | 3 cut(s) | 27, 408, 871 |
| SfcI | CTRYAG | 2 cut(s) | 358, 987 |
| SinI | GGWCC | 3 cut(s) | 628, 667, 966 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 644, 743 |
| Sse9I | AATT | 4 cut(s) | 752, 761, 888, 1084 |
| SspMI | CTAG | 2 cut(s) | 656, 873 |
| StyD4I | CCNGG | 5 cut(s) | 180, 607, 630, 907, 962 |
| StyI | CCWWGG | 1 cut(s) | 933 |
| TaaI | ACNGT | 3 cut(s) | 92, 362, 830 |
| TaiI | ACGT | 4 cut(s) | 482, 540, 558, 1126 |
| TaqI | TCGA | 3 cut(s) | 273, 756, 832 |
| TasI | AATT | 4 cut(s) | 752, 761, 888, 1084 |
| TatI | WGTACW | 2 cut(s) | 144, 161 |
| TauI | GCSGC | 2 cut(s) | 224, 596 |
| Tru1I | TTAA | 4 cut(s) | 75, 335, 558, 764 |
| Tru9I | TTAA | 4 cut(s) | 75, 335, 558, 764 |
| TscAI | CASTG | 2 cut(s) | 18, 552 |
| TseFI | GTSAC | 2 cut(s) | 151, 1027 |
| TseI | GCWGC | 1 cut(s) | 530 |
| Tsp45I | GTSAC | 2 cut(s) | 151, 1027 |
| TspDTI | ATGAA | 1 cut(s) | 579 |
| TspRI | CASTG | 2 cut(s) | 18, 552 |
| Tth111I | GACNNNGTC | 1 cut(s) | 828 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 244 |
| VpaK11BI | GGWCC | 3 cut(s) | 628, 667, 966 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 334, 674 |
| XapI | RAATTY | 2 cut(s) | 752, 1084 |
| XceI | RCATGY | 1 cut(s) | 1046 |
| XmiI | GTMKAC | 3 cut(s) | 6, 616, 832 |
| XmnI | GAANNNNTTC | 1 cut(s) | 479 |
| XspI | CTAG | 2 cut(s) | 656, 873 |