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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 101 |
| AccII | CGCG | 1 cut(s) | 505 |
| AciI | CCGC | 7 cut(s) | 248, 299, 441, 462, 503, 563, 604 |
| AclWI | GGATC | 2 cut(s) | 48, 258 |
| AcoI | YGGCCR | 1 cut(s) | 318 |
| AcsI | RAATTY | 1 cut(s) | 335 |
| AcuI | CTGAAG | 1 cut(s) | 497 |
| AfaI | GTAC | 1 cut(s) | 637 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 208, 382, 383 |
| AluBI | AGCT | 6 cut(s) | 80, 198, 238, 346, 365, 437 |
| AluI | AGCT | 6 cut(s) | 80, 198, 238, 346, 365, 437 |
| Alw26I | GTCTC | 1 cut(s) | 296 |
| AlwI | GGATC | 2 cut(s) | 48, 258 |
| AoxI | GGCC | 3 cut(s) | 206, 318, 597 |
| ApeKI | GCWGC | 3 cut(s) | 198, 224, 560 |
| ApoI | RAATTY | 1 cut(s) | 335 |
| AspLEI | GCGC | 3 cut(s) | 26, 94, 507 |
| AspS9I | GGNCC | 1 cut(s) | 450 |
| AsuC2I | CCSGG | 1 cut(s) | 210 |
| AsuHPI | GGTGA | 1 cut(s) | 410 |
| AsuNHI | GCTAGC | 1 cut(s) | 76 |
| AvaII | GGWCC | 1 cut(s) | 450 |
| AxyI | CCTNAGG | 1 cut(s) | 129 |
| BaeGI | GKGCMC | 2 cut(s) | 358, 680 |
| BalI | TGGCCA | 1 cut(s) | 320 |
| BanI | GGYRCC | 1 cut(s) | 101 |
| BbvI | GCAGC | 3 cut(s) | 185, 211, 572 |
| BccI | CCATC | 1 cut(s) | 404 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 597, 631 |
| BcnI | CCSGG | 1 cut(s) | 210 |
| BcoDI | GTCTC | 1 cut(s) | 296 |
| BfaI | CTAG | 4 cut(s) | 77, 149, 189, 359 |
| BfoI | RGCGCY | 1 cut(s) | 27 |
| BisI | GCNGC | 3 cut(s) | 199, 225, 561 |
| BlsI | GCNGC | 3 cut(s) | 200, 226, 562 |
| Bme1390I | CCNGG | 1 cut(s) | 210 |
| Bme18I | GGWCC | 1 cut(s) | 450 |
| BmgT120I | GGNCC | 1 cut(s) | 450 |
| BmiI | GGNNCC | 1 cut(s) | 103 |
| BmrFI | CCNGG | 1 cut(s) | 210 |
| BmsI | GCATC | 1 cut(s) | 424 |
| BmtI | GCTAGC | 1 cut(s) | 80 |
| BoxI | GACNNNNGTC | 1 cut(s) | 395 |
| BpuEI | CTTGAG | 2 cut(s) | 123, 574 |
| BpuMI | CCSGG | 1 cut(s) | 210 |
| BsaBI | GATNNNNATC | 1 cut(s) | 414 |
| BsaI | GGTCTC | 1 cut(s) | 296 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 555 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 208, 382, 383 |
| Bse21I | CCTNAGG | 1 cut(s) | 129 |
| Bse8I | GATNNNNATC | 1 cut(s) | 414 |
| BseDI | CCNNGG | 2 cut(s) | 105, 555 |
| BseJI | GATNNNNATC | 1 cut(s) | 414 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 208, 382, 383 |
| BseMII | CTCAG | 1 cut(s) | 143 |
| BseSI | GKGCMC | 2 cut(s) | 358, 680 |
| BseXI | GCAGC | 3 cut(s) | 185, 211, 572 |
| Bsh1236I | CGCG | 1 cut(s) | 505 |
| BshFI | GGCC | 3 cut(s) | 208, 320, 599 |
| BshNI | GGYRCC | 1 cut(s) | 101 |
| BsiSI | CCGG | 1 cut(s) | 209 |
| BslFI | GGGAC | 1 cut(s) | 225 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 208, 382, 383 |
| BsmAI | GTCTC | 1 cut(s) | 296 |
| BsmFI | GGGAC | 1 cut(s) | 225 |
| BsnI | GGCC | 3 cut(s) | 208, 320, 599 |
| Bso31I | GGTCTC | 1 cut(s) | 296 |
| Bsp1286I | GDGCHC | 2 cut(s) | 358, 680 |
| Bsp143I | GATC | 3 cut(s) | 31, 40, 250 |
| BspACI | CCGC | 7 cut(s) | 248, 299, 441, 462, 503, 563, 604 |
| BspANI | GGCC | 3 cut(s) | 208, 320, 599 |
| BspCNI | CTCAG | 1 cut(s) | 142 |
| BspFNI | CGCG | 1 cut(s) | 505 |
| BspLI | GGNNCC | 1 cut(s) | 103 |
| BspOI | GCTAGC | 1 cut(s) | 80 |
| BspPI | GGATC | 2 cut(s) | 48, 258 |
| BspT107I | GGYRCC | 1 cut(s) | 101 |
| BspTNI | GGTCTC | 1 cut(s) | 296 |
| BssECI | CCNNGG | 2 cut(s) | 105, 555 |
| BssMI | GATC | 3 cut(s) | 31, 40, 250 |
| BssT1I | CCWWGG | 1 cut(s) | 105 |
| Bst4CI | ACNGT | 4 cut(s) | 89, 330, 536, 749 |
| Bst6I | CTCTTC | 1 cut(s) | 643 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 198 |
| BstC8I | GCNNGC | 3 cut(s) | 78, 240, 439 |
| BstDEI | CTNAG | 1 cut(s) | 129 |
| BstEII | GGTNACC | 1 cut(s) | 565 |
| BstFNI | CGCG | 1 cut(s) | 505 |
| BstH2I | RGCGCY | 1 cut(s) | 27 |
| BstHHI | GCGC | 3 cut(s) | 26, 94, 507 |
| BstKTI | GATC | 3 cut(s) | 34, 43, 253 |
| BstMAI | GTCTC | 1 cut(s) | 296 |
| BstMBI | GATC | 3 cut(s) | 31, 40, 250 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 21, 198, 362 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 395 |
| BstPI | GGTNACC | 1 cut(s) | 565 |
| BstSCI | CCNGG | 1 cut(s) | 208 |
| BstSLI | GKGCMC | 2 cut(s) | 358, 680 |
| BstUI | CGCG | 1 cut(s) | 505 |
| BstV1I | GCAGC | 3 cut(s) | 185, 211, 572 |
| BstX2I | RGATCY | 1 cut(s) | 250 |
| BstYI | RGATCY | 1 cut(s) | 250 |
| Bsu36I | CCTNAGG | 1 cut(s) | 129 |
| BsuRI | GGCC | 3 cut(s) | 208, 320, 599 |
| BtgZI | GCGATG | 1 cut(s) | 713 |
| BtsI | GCAGTG | 1 cut(s) | 613 |
| BtsIMutI | CAGTG | 4 cut(s) | 177, 326, 541, 613 |
| Cac8I | GCNNGC | 3 cut(s) | 78, 240, 439 |
| CfoI | GCGC | 3 cut(s) | 26, 94, 507 |
| Cfr13I | GGNCC | 1 cut(s) | 450 |
| Csp6I | GTAC | 1 cut(s) | 636 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 517, 552 |
| CviAII | CATG | 2 cut(s) | 454, 718 |
| CviQI | GTAC | 1 cut(s) | 636 |
| DdeI | CTNAG | 1 cut(s) | 129 |
| DpnI | GATC | 3 cut(s) | 33, 42, 252 |
| DpnII | GATC | 3 cut(s) | 31, 40, 250 |
| EaeI | YGGCCR | 1 cut(s) | 318 |
| Eam1104I | CTCTTC | 1 cut(s) | 643 |
| EarI | CTCTTC | 1 cut(s) | 643 |
| EciI | GGCGGA | 3 cut(s) | 263, 314, 451 |
| Eco130I | CCWWGG | 1 cut(s) | 105 |
| Eco147I | AGGCCT | 1 cut(s) | 599 |
| Eco31I | GGTCTC | 1 cut(s) | 296 |
| Eco47I | GGWCC | 1 cut(s) | 450 |
| Eco57I | CTGAAG | 1 cut(s) | 497 |
| Eco81I | CCTNAGG | 1 cut(s) | 129 |
| Eco91I | GGTNACC | 1 cut(s) | 565 |
| EcoO65I | GGTNACC | 1 cut(s) | 565 |
| EcoT14I | CCWWGG | 1 cut(s) | 105 |
| ErhI | CCWWGG | 1 cut(s) | 105 |
| FaeI | CATG | 2 cut(s) | 457, 721 |
| FaiI | YATR | 4 cut(s) | 258, 455, 546, 719 |
| FaqI | GGGAC | 1 cut(s) | 225 |
| FatI | CATG | 2 cut(s) | 453, 717 |
| FauI | CCCGC | 2 cut(s) | 434, 510 |
| Fnu4HI | GCNGC | 3 cut(s) | 199, 225, 561 |
| Fsp4HI | GCNGC | 3 cut(s) | 199, 225, 561 |
| FspBI | CTAG | 4 cut(s) | 77, 149, 189, 359 |
| GlaI | GCGC | 3 cut(s) | 25, 93, 506 |
| GluI | GCNGC | 3 cut(s) | 199, 225, 561 |
| HaeII | RGCGCY | 1 cut(s) | 27 |
| HaeIII | GGCC | 3 cut(s) | 208, 320, 599 |
| HapII | CCGG | 1 cut(s) | 209 |
| HhaI | GCGC | 3 cut(s) | 26, 94, 507 |
| Hin1II | CATG | 2 cut(s) | 457, 721 |
| Hin6I | GCGC | 3 cut(s) | 24, 92, 505 |
| HinP1I | GCGC | 3 cut(s) | 24, 92, 505 |
| HincII | GTYRAC | 2 cut(s) | 125, 688 |
| HindII | GTYRAC | 2 cut(s) | 125, 688 |
| HinfI | GANTC | 2 cut(s) | 154, 736 |
| HpaII | CCGG | 1 cut(s) | 209 |
| HphI | GGTGA | 1 cut(s) | 410 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 125, 688 |
| Hpy188III | TCNNGA | 3 cut(s) | 131, 140, 149 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 125, 688 |
| Hpy99I | CGWCG | 5 cut(s) | 32, 119, 122, 344, 401 |
| HpyAV | CCTTC | 2 cut(s) | 282, 289 |
| HpyCH4III | ACNGT | 4 cut(s) | 89, 330, 536, 749 |
| HpyCH4IV | ACGT | 3 cut(s) | 114, 396, 684 |
| HpyCH4V | TGCA | 2 cut(s) | 256, 743 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 21, 198, 362 |
| HpyF3I | CTNAG | 1 cut(s) | 129 |
| HpySE526I | ACGT | 3 cut(s) | 114, 396, 684 |
| Hsp92II | CATG | 2 cut(s) | 457, 721 |
| HspAI | GCGC | 3 cut(s) | 24, 92, 505 |
| Kzo9I | GATC | 3 cut(s) | 31, 40, 250 |
| LmnI | GCTCC | 1 cut(s) | 362 |
| Lsp1109I | GCAGC | 3 cut(s) | 185, 211, 572 |
| LweI | GCATC | 1 cut(s) | 424 |
| MaeI | CTAG | 4 cut(s) | 77, 149, 189, 359 |
| MaeII | ACGT | 3 cut(s) | 114, 396, 684 |
| MaeIII | GTNAC | 2 cut(s) | 493, 565 |
| MalI | GATC | 3 cut(s) | 33, 42, 252 |
| MboI | GATC | 3 cut(s) | 31, 40, 250 |
| MboII | GAAGA | 2 cut(s) | 660, 695 |
| MfeI | CAATTG | 1 cut(s) | 642 |
| MflI | RGATCY | 1 cut(s) | 250 |
| MhlI | GDGCHC | 2 cut(s) | 358, 680 |
| MlsI | TGGCCA | 1 cut(s) | 320 |
| MluCI | AATT | 4 cut(s) | 335, 541, 622, 642 |
| MluNI | TGGCCA | 1 cut(s) | 320 |
| MnlI | CCTC | 6 cut(s) | 138, 168, 230, 469, 565, 646 |
| Mox20I | TGGCCA | 1 cut(s) | 320 |
| MscI | TGGCCA | 1 cut(s) | 320 |
| MseI | TTAA | 1 cut(s) | 423 |
| MslI | CAYNNNNRTG | 1 cut(s) | 716 |
| Msp20I | TGGCCA | 1 cut(s) | 320 |
| MspA1I | CMGCKG | 2 cut(s) | 198, 563 |
| MspI | CCGG | 1 cut(s) | 209 |
| MspR9I | CCNGG | 1 cut(s) | 210 |
| MunI | CAATTG | 1 cut(s) | 642 |
| MvnI | CGCG | 1 cut(s) | 505 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 21, 198, 362 |
| NciI | CCSGG | 1 cut(s) | 210 |
| NdeII | GATC | 3 cut(s) | 31, 40, 250 |
| NheI | GCTAGC | 1 cut(s) | 76 |
| NlaIII | CATG | 2 cut(s) | 457, 721 |
| NlaIV | GGNNCC | 1 cut(s) | 103 |
| NmeAIII | GCCGAG | 1 cut(s) | 536 |
| NmuCI | GTSAC | 1 cut(s) | 493 |
| PceI | AGGCCT | 1 cut(s) | 599 |
| PfeI | GAWTC | 2 cut(s) | 154, 736 |
| PflFI | GACNNNGTC | 1 cut(s) | 454 |
| PkrI | GCNGC | 3 cut(s) | 200, 226, 562 |
| PshAI | GACNNNNGTC | 1 cut(s) | 395 |
| PspEI | GGTNACC | 1 cut(s) | 565 |
| PspN4I | GGNNCC | 1 cut(s) | 103 |
| PspPI | GGNCC | 1 cut(s) | 450 |
| PsuI | RGATCY | 1 cut(s) | 250 |
| PsyI | GACNNNGTC | 1 cut(s) | 454 |
| PvuII | CAGCTG | 1 cut(s) | 198 |
| RsaI | GTAC | 1 cut(s) | 637 |
| RsaNI | GTAC | 1 cut(s) | 636 |
| RseI | CAYNNNNRTG | 1 cut(s) | 716 |
| SaqAI | TTAA | 1 cut(s) | 423 |
| SatI | GCNGC | 3 cut(s) | 199, 225, 561 |
| Sau3AI | GATC | 3 cut(s) | 31, 40, 250 |
| Sau96I | GGNCC | 1 cut(s) | 450 |
| ScrFI | CCNGG | 1 cut(s) | 210 |
| SduI | GDGCHC | 2 cut(s) | 358, 680 |
| SfaNI | GCATC | 1 cut(s) | 424 |
| SinI | GGWCC | 1 cut(s) | 450 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 716 |
| SmlI | CTYRAG | 2 cut(s) | 138, 589 |
| SmoI | CTYRAG | 2 cut(s) | 138, 589 |
| Sse9I | AATT | 4 cut(s) | 335, 541, 622, 642 |
| SseBI | AGGCCT | 1 cut(s) | 599 |
| SsiI | CCGC | 7 cut(s) | 248, 299, 441, 462, 503, 563, 604 |
| SspMI | CTAG | 4 cut(s) | 77, 149, 189, 359 |
| StuI | AGGCCT | 1 cut(s) | 599 |
| StyD4I | CCNGG | 1 cut(s) | 208 |
| StyI | CCWWGG | 1 cut(s) | 105 |
| TaaI | ACNGT | 4 cut(s) | 89, 330, 536, 749 |
| TaiI | ACGT | 3 cut(s) | 117, 399, 687 |
| TaqI | TCGA | 4 cut(s) | 160, 339, 399, 524 |
| TaqII | GACCGA | 1 cut(s) | 110 |
| TasI | AATT | 4 cut(s) | 335, 541, 622, 642 |
| TfiI | GAWTC | 2 cut(s) | 154, 736 |
| Tru1I | TTAA | 1 cut(s) | 423 |
| Tru9I | TTAA | 1 cut(s) | 423 |
| TscAI | CASTG | 4 cut(s) | 177, 333, 541, 620 |
| TseFI | GTSAC | 1 cut(s) | 493 |
| TseI | GCWGC | 3 cut(s) | 198, 224, 560 |
| Tsp45I | GTSAC | 1 cut(s) | 493 |
| TspDTI | ATGAA | 1 cut(s) | 716 |
| TspRI | CASTG | 4 cut(s) | 177, 333, 541, 620 |
| Tth111I | GACNNNGTC | 1 cut(s) | 454 |
| VpaK11BI | GGWCC | 1 cut(s) | 450 |
| XapI | RAATTY | 1 cut(s) | 335 |
| XbaI | TCTAGA | 1 cut(s) | 148 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 235 |
| XspI | CTAG | 4 cut(s) | 77, 149, 189, 359 |