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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 445, 614 |
| Acc36I | ACCTGC | 2 cut(s) | 445, 614 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 138 |
| AccBSI | CCGCTC | 1 cut(s) | 652 |
| AccII | CGCG | 1 cut(s) | 104 |
| AclWI | GGATC | 2 cut(s) | 279, 695 |
| AcoI | YGGCCR | 2 cut(s) | 614, 750 |
| AcsI | RAATTY | 1 cut(s) | 476 |
| AcuI | CTGAAG | 1 cut(s) | 167 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 45, 46, 138, 400, 454, 619, 623 |
| AgsI | TTSAA | 2 cut(s) | 481, 780 |
| AjnI | CCWGG | 1 cut(s) | 616 |
| AleI | CACNNNNGTG | 1 cut(s) | 317 |
| AluBI | AGCT | 4 cut(s) | 129, 308, 379, 487 |
| AluI | AGCT | 4 cut(s) | 129, 308, 379, 487 |
| Alw21I | GWGCWC | 1 cut(s) | 417 |
| AlwI | GGATC | 2 cut(s) | 279, 695 |
| AoxI | GGCC | 5 cut(s) | 208, 352, 438, 614, 750 |
| ApeKI | GCWGC | 2 cut(s) | 336, 589 |
| ApoI | RAATTY | 1 cut(s) | 476 |
| AspLEI | GCGC | 2 cut(s) | 106, 429 |
| AspS9I | GGNCC | 3 cut(s) | 391, 512, 671 |
| AsuC2I | CCSGG | 2 cut(s) | 78, 669 |
| AsuHPI | GGTGA | 5 cut(s) | 62, 75, 86, 220, 670 |
| AvaII | GGWCC | 3 cut(s) | 391, 512, 671 |
| BalI | TGGCCA | 1 cut(s) | 752 |
| Bbv12I | GWGCWC | 1 cut(s) | 417 |
| BbvCI | CCTCAGC | 1 cut(s) | 258 |
| BbvI | GCAGC | 2 cut(s) | 348, 601 |
| BccI | CCATC | 4 cut(s) | 110, 205, 219, 823 |
| BceAI | ACGGC | 2 cut(s) | 683, 760 |
| BciT130I | CCWGG | 1 cut(s) | 618 |
| BcnI | CCSGG | 2 cut(s) | 78, 669 |
| BfaI | CTAG | 3 cut(s) | 350, 663, 684 |
| BfuAI | ACCTGC | 2 cut(s) | 445, 614 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 351 |
| BglII | AGATCT | 1 cut(s) | 358 |
| BisI | GCNGC | 4 cut(s) | 337, 366, 564, 590 |
| BlsI | GCNGC | 4 cut(s) | 338, 367, 565, 591 |
| Bme1390I | CCNGG | 3 cut(s) | 78, 618, 669 |
| Bme18I | GGWCC | 3 cut(s) | 391, 512, 671 |
| BmgT120I | GGNCC | 3 cut(s) | 391, 512, 671 |
| BmiI | GGNNCC | 2 cut(s) | 514, 672 |
| BmrFI | CCNGG | 3 cut(s) | 78, 618, 669 |
| BmrI | ACTGGG | 1 cut(s) | 626 |
| BmsI | GCATC | 3 cut(s) | 129, 576, 720 |
| BmuI | ACTGGG | 1 cut(s) | 626 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 1 cut(s) | 115 |
| Bpu10I | CCTNAGC | 2 cut(s) | 71, 258 |
| BpuMI | CCSGG | 2 cut(s) | 78, 669 |
| BsaJI | CCNNGG | 3 cut(s) | 24, 76, 617 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 45, 46, 138, 400, 454, 619, 623 |
| Bse1I | ACTGG | 1 cut(s) | 632 |
| BseBI | CCWGG | 1 cut(s) | 618 |
| BseDI | CCNNGG | 3 cut(s) | 24, 76, 617 |
| BseGI | GGATG | 3 cut(s) | 211, 591, 815 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 45, 46, 138, 400, 454, 619, 623 |
| BseMII | CTCAG | 1 cut(s) | 249 |
| BseNI | ACTGG | 1 cut(s) | 632 |
| BseRI | GAGGAG | 1 cut(s) | 275 |
| BseXI | GCAGC | 2 cut(s) | 348, 601 |
| BseYI | CCCAGC | 1 cut(s) | 487 |
| Bsh1236I | CGCG | 1 cut(s) | 104 |
| Bsh1285I | CGRYCG | 1 cut(s) | 575 |
| BshFI | GGCC | 5 cut(s) | 210, 354, 440, 616, 752 |
| BsiEI | CGRYCG | 1 cut(s) | 575 |
| BsiHKAI | GWGCWC | 1 cut(s) | 417 |
| BsiSI | CCGG | 3 cut(s) | 78, 613, 668 |
| BslFI | GGGAC | 3 cut(s) | 144, 614, 684 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 45, 46, 138, 400, 454, 619, 623 |
| BsmFI | GGGAC | 3 cut(s) | 144, 614, 684 |
| BsnI | GGCC | 5 cut(s) | 210, 354, 440, 616, 752 |
| Bsp1286I | GDGCHC | 1 cut(s) | 417 |
| Bsp143I | GATC | 4 cut(s) | 182, 271, 358, 687 |
| BspANI | GGCC | 5 cut(s) | 210, 354, 440, 616, 752 |
| BspCNI | CTCAG | 1 cut(s) | 250 |
| BspFNI | CGCG | 1 cut(s) | 104 |
| BspLI | GGNNCC | 2 cut(s) | 514, 672 |
| BspMI | ACCTGC | 2 cut(s) | 445, 614 |
| BspPI | GGATC | 2 cut(s) | 279, 695 |
| BsrBI | CCGCTC | 1 cut(s) | 652 |
| BsrI | ACTGG | 1 cut(s) | 632 |
| BssECI | CCNNGG | 3 cut(s) | 24, 76, 617 |
| BssMI | GATC | 4 cut(s) | 182, 271, 358, 687 |
| BssT1I | CCWWGG | 1 cut(s) | 24 |
| Bst2UI | CCWGG | 1 cut(s) | 618 |
| Bst4CI | ACNGT | 2 cut(s) | 242, 576 |
| Bst6I | CTCTTC | 1 cut(s) | 173 |
| BstC8I | GCNNGC | 4 cut(s) | 267, 381, 568, 696 |
| BstDEI | CTNAG | 2 cut(s) | 71, 258 |
| BstEII | GGTNACC | 1 cut(s) | 50 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 398 |
| BstF5I | GGATG | 3 cut(s) | 211, 591, 815 |
| BstFNI | CGCG | 1 cut(s) | 104 |
| BstHHI | GCGC | 2 cut(s) | 106, 429 |
| BstKTI | GATC | 4 cut(s) | 185, 274, 361, 690 |
| BstMBI | GATC | 4 cut(s) | 182, 271, 358, 687 |
| BstMCI | CGRYCG | 1 cut(s) | 575 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 126, 314, 323, 342, 351, 598, 613 |
| BstNI | CCWGG | 1 cut(s) | 618 |
| BstPI | GGTNACC | 1 cut(s) | 50 |
| BstSCI | CCNGG | 3 cut(s) | 76, 616, 667 |
| BstUI | CGCG | 1 cut(s) | 104 |
| BstV1I | GCAGC | 2 cut(s) | 348, 601 |
| BstX2I | RGATCY | 2 cut(s) | 358, 687 |
| BstYI | RGATCY | 2 cut(s) | 358, 687 |
| BsuRI | GGCC | 5 cut(s) | 210, 354, 440, 616, 752 |
| BtsCI | GGATG | 3 cut(s) | 211, 591, 815 |
| BtsI | GCAGTG | 1 cut(s) | 312 |
| BtsIMutI | CAGTG | 2 cut(s) | 312, 639 |
| BveI | ACCTGC | 2 cut(s) | 445, 614 |
| Cac8I | GCNNGC | 4 cut(s) | 267, 381, 568, 696 |
| CfoI | GCGC | 2 cut(s) | 106, 429 |
| Cfr13I | GGNCC | 3 cut(s) | 391, 512, 671 |
| CviAII | CATG | 5 cut(s) | 14, 174, 232, 748, 768 |
| DdeI | CTNAG | 2 cut(s) | 71, 258 |
| DpnI | GATC | 4 cut(s) | 184, 273, 360, 689 |
| DpnII | GATC | 4 cut(s) | 182, 271, 358, 687 |
| EaeI | YGGCCR | 2 cut(s) | 614, 750 |
| Eam1104I | CTCTTC | 1 cut(s) | 173 |
| EarI | CTCTTC | 1 cut(s) | 173 |
| EciI | GGCGGA | 1 cut(s) | 404 |
| Eco130I | CCWWGG | 1 cut(s) | 24 |
| Eco47I | GGWCC | 3 cut(s) | 391, 512, 671 |
| Eco57I | CTGAAG | 1 cut(s) | 167 |
| Eco91I | GGTNACC | 1 cut(s) | 50 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 398 |
| EcoO65I | GGTNACC | 1 cut(s) | 50 |
| EcoRII | CCWGG | 1 cut(s) | 616 |
| EcoT14I | CCWWGG | 1 cut(s) | 24 |
| ErhI | CCWWGG | 1 cut(s) | 24 |
| FaeI | CATG | 5 cut(s) | 17, 177, 235, 751, 771 |
| FaqI | GGGAC | 3 cut(s) | 144, 614, 684 |
| FatI | CATG | 5 cut(s) | 13, 173, 231, 747, 767 |
| FauI | CCCGC | 3 cut(s) | 47, 239, 537 |
| FauNDI | CATATG | 1 cut(s) | 712 |
| Fnu4HI | GCNGC | 4 cut(s) | 337, 366, 564, 590 |
| FokI | GGATG | 3 cut(s) | 198, 598, 802 |
| Fsp4HI | GCNGC | 4 cut(s) | 337, 366, 564, 590 |
| FspBI | CTAG | 3 cut(s) | 350, 663, 684 |
| GlaI | GCGC | 2 cut(s) | 105, 428 |
| GluI | GCNGC | 4 cut(s) | 337, 366, 564, 590 |
| GsaI | CCCAGC | 1 cut(s) | 491 |
| GsuI | CTGGAG | 1 cut(s) | 115 |
| HaeIII | GGCC | 5 cut(s) | 210, 354, 440, 616, 752 |
| HapII | CCGG | 3 cut(s) | 78, 613, 668 |
| HhaI | GCGC | 2 cut(s) | 106, 429 |
| Hin1II | CATG | 5 cut(s) | 17, 177, 235, 751, 771 |
| Hin6I | GCGC | 2 cut(s) | 104, 427 |
| HinP1I | GCGC | 2 cut(s) | 104, 427 |
| HinfI | GANTC | 1 cut(s) | 803 |
| HpaII | CCGG | 3 cut(s) | 78, 613, 668 |
| HphI | GGTGA | 5 cut(s) | 62, 75, 86, 220, 670 |
| Hpy166II | GTNNAC | 1 cut(s) | 765 |
| Hpy188I | TCNGA | 2 cut(s) | 125, 704 |
| Hpy188III | TCNNGA | 4 cut(s) | 113, 132, 663, 780 |
| Hpy8I | GTNNAC | 1 cut(s) | 765 |
| HpyAV | CCTTC | 4 cut(s) | 129, 430, 777, 786 |
| HpyCH4III | ACNGT | 2 cut(s) | 242, 576 |
| HpyCH4V | TGCA | 3 cut(s) | 589, 694, 716 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 126, 314, 323, 342, 351, 598, 613 |
| HpyF3I | CTNAG | 2 cut(s) | 71, 258 |
| Hsp92II | CATG | 5 cut(s) | 17, 177, 235, 751, 771 |
| HspAI | GCGC | 2 cut(s) | 104, 427 |
| Kzo9I | GATC | 4 cut(s) | 182, 271, 358, 687 |
| LmnI | GCTCC | 2 cut(s) | 134, 262 |
| Lsp1109I | GCAGC | 2 cut(s) | 348, 601 |
| LweI | GCATC | 3 cut(s) | 129, 576, 720 |
| MaeI | CTAG | 3 cut(s) | 350, 663, 684 |
| MaeIII | GTNAC | 3 cut(s) | 50, 460, 786 |
| MalI | GATC | 4 cut(s) | 184, 273, 360, 689 |
| MbiI | CCGCTC | 1 cut(s) | 652 |
| MboI | GATC | 4 cut(s) | 182, 271, 358, 687 |
| MboII | GAAGA | 1 cut(s) | 190 |
| MflI | RGATCY | 2 cut(s) | 358, 687 |
| MhlI | GDGCHC | 1 cut(s) | 417 |
| MlsI | TGGCCA | 1 cut(s) | 752 |
| MluCI | AATT | 3 cut(s) | 34, 476, 593 |
| MluNI | TGGCCA | 1 cut(s) | 752 |
| MnlI | CCTC | 9 cut(s) | 97, 253, 316, 404, 529, 576, 769, 829, 846 |
| Mox20I | TGGCCA | 1 cut(s) | 752 |
| MscI | TGGCCA | 1 cut(s) | 752 |
| MseI | TTAA | 1 cut(s) | 534 |
| MslI | CAYNNNNRTG | 4 cut(s) | 12, 114, 317, 467 |
| Msp20I | TGGCCA | 1 cut(s) | 752 |
| MspA1I | CMGCKG | 1 cut(s) | 487 |
| MspI | CCGG | 3 cut(s) | 78, 613, 668 |
| MspR9I | CCNGG | 3 cut(s) | 78, 618, 669 |
| MvaI | CCWGG | 1 cut(s) | 618 |
| MvnI | CGCG | 1 cut(s) | 104 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 126, 314, 323, 342, 351, 598, 613 |
| NciI | CCSGG | 2 cut(s) | 78, 669 |
| NdeI | CATATG | 1 cut(s) | 712 |
| NdeII | GATC | 4 cut(s) | 182, 271, 358, 687 |
| NlaIII | CATG | 5 cut(s) | 17, 177, 235, 751, 771 |
| NlaIV | GGNNCC | 2 cut(s) | 514, 672 |
| NmeAIII | GCCGAG | 1 cut(s) | 380 |
| NmuCI | GTSAC | 3 cut(s) | 50, 460, 786 |
| OliI | CACNNNNGTG | 1 cut(s) | 317 |
| PaqCI | CACCTGC | 2 cut(s) | 445, 614 |
| PfeI | GAWTC | 1 cut(s) | 803 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 138 |
| PkrI | GCNGC | 4 cut(s) | 338, 367, 565, 591 |
| Psp6I | CCWGG | 1 cut(s) | 616 |
| PspEI | GGTNACC | 1 cut(s) | 50 |
| PspFI | CCCAGC | 1 cut(s) | 487 |
| PspGI | CCWGG | 1 cut(s) | 616 |
| PspN4I | GGNNCC | 2 cut(s) | 514, 672 |
| PspPI | GGNCC | 3 cut(s) | 391, 512, 671 |
| PsuI | RGATCY | 2 cut(s) | 358, 687 |
| PvuII | CAGCTG | 1 cut(s) | 487 |
| RseI | CAYNNNNRTG | 4 cut(s) | 12, 114, 317, 467 |
| SaqAI | TTAA | 1 cut(s) | 534 |
| SatI | GCNGC | 4 cut(s) | 337, 366, 564, 590 |
| Sau3AI | GATC | 4 cut(s) | 182, 271, 358, 687 |
| Sau96I | GGNCC | 3 cut(s) | 391, 512, 671 |
| ScrFI | CCNGG | 3 cut(s) | 78, 618, 669 |
| SduI | GDGCHC | 1 cut(s) | 417 |
| SfaNI | GCATC | 3 cut(s) | 129, 576, 720 |
| SinI | GGWCC | 3 cut(s) | 391, 512, 671 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 12, 114, 317, 467 |
| Sse9I | AATT | 3 cut(s) | 34, 476, 593 |
| SspMI | CTAG | 3 cut(s) | 350, 663, 684 |
| StyD4I | CCNGG | 3 cut(s) | 76, 616, 667 |
| StyI | CCWWGG | 1 cut(s) | 24 |
| TaaI | ACNGT | 2 cut(s) | 242, 576 |
| TaqI | TCGA | 1 cut(s) | 167 |
| TasI | AATT | 3 cut(s) | 34, 476, 593 |
| TauI | GCSGC | 2 cut(s) | 368, 566 |
| TfiI | GAWTC | 1 cut(s) | 803 |
| Tru1I | TTAA | 1 cut(s) | 534 |
| Tru9I | TTAA | 1 cut(s) | 534 |
| TscAI | CASTG | 2 cut(s) | 319, 639 |
| TseFI | GTSAC | 3 cut(s) | 50, 460, 786 |
| TseI | GCWGC | 2 cut(s) | 336, 589 |
| Tsp45I | GTSAC | 3 cut(s) | 50, 460, 786 |
| TspGWI | ACGGA | 1 cut(s) | 73 |
| TspRI | CASTG | 2 cut(s) | 319, 639 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 138 |
| VpaK11BI | GGWCC | 3 cut(s) | 391, 512, 671 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 398 |
| XapI | RAATTY | 1 cut(s) | 476 |
| XbaI | TCTAGA | 1 cut(s) | 662 |
| XspI | CTAG | 3 cut(s) | 350, 663, 684 |