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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 406 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 1 cut(s) | 90 |
| AccIII | TCCGGA | 1 cut(s) | 645 |
| AclWI | GGATC | 2 cut(s) | 201, 637 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 2 cut(s) | 264, 546 |
| AcyI | GRCGYC | 1 cut(s) | 660 |
| AfaI | GTAC | 3 cut(s) | 176, 381, 408 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 77, 151, 256, 368, 610 |
| AjnI | CCWGG | 2 cut(s) | 347, 382 |
| AluBI | AGCT | 1 cut(s) | 181 |
| AluI | AGCT | 1 cut(s) | 181 |
| AlwI | GGATC | 2 cut(s) | 201, 637 |
| Aor13HI | TCCGGA | 1 cut(s) | 645 |
| AoxI | GGCC | 3 cut(s) | 108, 154, 306 |
| ApeKI | GCWGC | 1 cut(s) | 141 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 4 cut(s) | 92, 231, 552, 670 |
| AspS9I | GGNCC | 3 cut(s) | 154, 306, 440 |
| AsuC2I | CCSGG | 4 cut(s) | 107, 117, 153, 562 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| AsuNHI | GCTAGC | 1 cut(s) | 181 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 363, 396 |
| BanI | GGYRCC | 2 cut(s) | 379, 406 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BccI | CCATC | 2 cut(s) | 51, 59 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 581, 615 |
| BciT130I | CCWGG | 2 cut(s) | 349, 384 |
| BcnI | CCSGG | 4 cut(s) | 107, 117, 153, 562 |
| BfaI | CTAG | 4 cut(s) | 164, 182, 513, 629 |
| BfoI | RGCGCY | 1 cut(s) | 671 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 3 cut(s) | 139, 142, 145 |
| BlsI | GCNGC | 3 cut(s) | 140, 143, 146 |
| Bme1390I | CCNGG | 6 cut(s) | 107, 117, 153, 349, 384, 562 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmgT120I | GGNCC | 3 cut(s) | 154, 306, 440 |
| BmiI | GGNNCC | 4 cut(s) | 307, 381, 408, 441 |
| BmrFI | CCNGG | 6 cut(s) | 107, 117, 153, 349, 384, 562 |
| BmsI | GCATC | 3 cut(s) | 390, 583, 609 |
| BmtI | GCTAGC | 1 cut(s) | 185 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| BpuMI | CCSGG | 4 cut(s) | 107, 117, 153, 562 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaHI | GRCGYC | 1 cut(s) | 660 |
| BsaJI | CCNNGG | 3 cut(s) | 105, 383, 545 |
| BsaWI | WCCGGW | 1 cut(s) | 645 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 77, 151, 256, 368, 610 |
| Bse118I | RCCGGY | 1 cut(s) | 368 |
| Bse1I | ACTGG | 1 cut(s) | 192 |
| BseAI | TCCGGA | 1 cut(s) | 645 |
| BseBI | CCWGG | 2 cut(s) | 349, 384 |
| BseDI | CCNNGG | 3 cut(s) | 105, 383, 545 |
| BseGI | GGATG | 3 cut(s) | 43, 51, 624 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 77, 151, 256, 368, 610 |
| BseMII | CTCAG | 1 cut(s) | 414 |
| BseNI | ACTGG | 1 cut(s) | 192 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| BseYI | CCCAGC | 4 cut(s) | 70, 301, 319, 664 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 3 cut(s) | 110, 156, 308 |
| BshNI | GGYRCC | 2 cut(s) | 379, 406 |
| BsiSI | CCGG | 6 cut(s) | 107, 117, 152, 369, 562, 646 |
| BslFI | GGGAC | 3 cut(s) | 453, 544, 647 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 77, 151, 256, 368, 610 |
| BsmFI | GGGAC | 3 cut(s) | 453, 544, 647 |
| BsnI | GGCC | 3 cut(s) | 110, 156, 308 |
| Bsp13I | TCCGGA | 1 cut(s) | 645 |
| Bsp143I | GATC | 3 cut(s) | 160, 193, 642 |
| BspANI | GGCC | 3 cut(s) | 110, 156, 308 |
| BspCNI | CTCAG | 1 cut(s) | 415 |
| BspEI | TCCGGA | 1 cut(s) | 645 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 4 cut(s) | 307, 381, 408, 441 |
| BspOI | GCTAGC | 1 cut(s) | 185 |
| BspPI | GGATC | 2 cut(s) | 201, 637 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 406 |
| BsrFI | RCCGGY | 1 cut(s) | 368 |
| BsrI | ACTGG | 1 cut(s) | 192 |
| BssAI | RCCGGY | 1 cut(s) | 368 |
| BssECI | CCNNGG | 3 cut(s) | 105, 383, 545 |
| BssMI | GATC | 3 cut(s) | 160, 193, 642 |
| BssNI | GRCGYC | 1 cut(s) | 660 |
| Bst2UI | CCWGG | 2 cut(s) | 349, 384 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstACI | GRCGYC | 1 cut(s) | 660 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 6 cut(s) | 36, 149, 183, 316, 332, 554 |
| BstDEI | CTNAG | 2 cut(s) | 423, 675 |
| BstF5I | GGATG | 3 cut(s) | 43, 51, 624 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 1 cut(s) | 671 |
| BstHHI | GCGC | 4 cut(s) | 92, 231, 552, 670 |
| BstKTI | GATC | 3 cut(s) | 163, 196, 645 |
| BstMBI | GATC | 3 cut(s) | 160, 193, 642 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 320, 562, 584, 593 |
| BstNI | CCWGG | 2 cut(s) | 349, 384 |
| BstSCI | CCNGG | 6 cut(s) | 105, 115, 151, 347, 382, 560 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 3 cut(s) | 110, 156, 308 |
| BtsCI | GGATG | 3 cut(s) | 43, 51, 624 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 410 |
| Cac8I | GCNNGC | 6 cut(s) | 36, 149, 183, 316, 332, 554 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 4 cut(s) | 92, 231, 552, 670 |
| Cfr10I | RCCGGY | 1 cut(s) | 368 |
| Cfr13I | GGNCC | 3 cut(s) | 154, 306, 440 |
| CseI | GACGC | 2 cut(s) | 307, 649 |
| Csp6I | GTAC | 3 cut(s) | 175, 380, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 1 cut(s) | 271 |
| CviQI | GTAC | 3 cut(s) | 175, 380, 407 |
| DdeI | CTNAG | 2 cut(s) | 423, 675 |
| DpnI | GATC | 3 cut(s) | 162, 195, 644 |
| DpnII | GATC | 3 cut(s) | 160, 193, 642 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| EciI | GGCGGA | 4 cut(s) | 206, 494, 525, 574 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco57I | CTGAAG | 2 cut(s) | 264, 546 |
| EcoRII | CCWGG | 2 cut(s) | 347, 382 |
| FaeI | CATG | 1 cut(s) | 274 |
| FaiI | YATR | 5 cut(s) | 173, 201, 272, 395, 452 |
| FaqI | GGGAC | 3 cut(s) | 453, 544, 647 |
| FatI | CATG | 1 cut(s) | 270 |
| FauI | CCCGC | 7 cut(s) | 8, 55, 156, 323, 396, 498, 558 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 3 cut(s) | 139, 142, 145 |
| FokI | GGATG | 3 cut(s) | 30, 38, 631 |
| Fsp4HI | GCNGC | 3 cut(s) | 139, 142, 145 |
| FspBI | CTAG | 4 cut(s) | 164, 182, 513, 629 |
| GlaI | GCGC | 4 cut(s) | 91, 230, 551, 669 |
| GluI | GCNGC | 3 cut(s) | 139, 142, 145 |
| GsaI | CCCAGC | 4 cut(s) | 74, 305, 323, 668 |
| HaeII | RGCGCY | 1 cut(s) | 671 |
| HaeIII | GGCC | 3 cut(s) | 110, 156, 308 |
| HapII | CCGG | 6 cut(s) | 107, 117, 152, 369, 562, 646 |
| HgaI | GACGC | 2 cut(s) | 307, 649 |
| HhaI | GCGC | 4 cut(s) | 92, 231, 552, 670 |
| Hin1I | GRCGYC | 1 cut(s) | 660 |
| Hin1II | CATG | 1 cut(s) | 274 |
| Hin6I | GCGC | 4 cut(s) | 90, 229, 550, 668 |
| HinP1I | GCGC | 4 cut(s) | 90, 229, 550, 668 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 3 cut(s) | 482, 581, 653 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 6 cut(s) | 107, 117, 152, 369, 562, 646 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 508 |
| Hpy188III | TCNNGA | 2 cut(s) | 271, 646 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy99I | CGWCG | 1 cut(s) | 593 |
| HpyAV | CCTTC | 1 cut(s) | 521 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 2 cut(s) | 217, 340 |
| HpyCH4V | TGCA | 2 cut(s) | 596, 622 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 320, 562, 584, 593 |
| HpyF3I | CTNAG | 2 cut(s) | 423, 675 |
| HpySE526I | ACGT | 2 cut(s) | 217, 340 |
| Hsp92I | GRCGYC | 1 cut(s) | 660 |
| Hsp92II | CATG | 1 cut(s) | 274 |
| HspAI | GCGC | 4 cut(s) | 90, 229, 550, 668 |
| Kpn2I | TCCGGA | 1 cut(s) | 645 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 160, 193, 642 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| LweI | GCATC | 3 cut(s) | 390, 583, 609 |
| MaeI | CTAG | 4 cut(s) | 164, 182, 513, 629 |
| MaeII | ACGT | 2 cut(s) | 217, 340 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 3 cut(s) | 162, 195, 644 |
| MboI | GATC | 3 cut(s) | 160, 193, 642 |
| MboII | GAAGA | 2 cut(s) | 188, 307 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MlyI | GAGTC | 1 cut(s) | 662 |
| MmeI | TCCRAC | 2 cut(s) | 90, 591 |
| MroI | TCCGGA | 1 cut(s) | 645 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 398 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 6 cut(s) | 107, 117, 152, 369, 562, 646 |
| MspR9I | CCNGG | 6 cut(s) | 107, 117, 153, 349, 384, 562 |
| MvaI | CCWGG | 2 cut(s) | 349, 384 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 320, 562, 584, 593 |
| NciI | CCSGG | 4 cut(s) | 107, 117, 153, 562 |
| NdeII | GATC | 3 cut(s) | 160, 193, 642 |
| NheI | GCTAGC | 1 cut(s) | 181 |
| NlaIII | CATG | 1 cut(s) | 274 |
| NlaIV | GGNNCC | 4 cut(s) | 307, 381, 408, 441 |
| NmeAIII | GCCGAG | 1 cut(s) | 570 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PfeI | GAWTC | 2 cut(s) | 482, 581 |
| PflFI | GACNNNGTC | 1 cut(s) | 652 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 3 cut(s) | 140, 143, 146 |
| PleI | GAGTC | 1 cut(s) | 661 |
| PpsI | GAGTC | 1 cut(s) | 661 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| Psp6I | CCWGG | 2 cut(s) | 347, 382 |
| PspFI | CCCAGC | 4 cut(s) | 70, 301, 319, 664 |
| PspGI | CCWGG | 2 cut(s) | 347, 382 |
| PspN4I | GGNNCC | 4 cut(s) | 307, 381, 408, 441 |
| PspPI | GGNCC | 3 cut(s) | 154, 306, 440 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PsyI | GACNNNGTC | 1 cut(s) | 652 |
| RsaI | GTAC | 3 cut(s) | 176, 381, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 380, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 398 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 3 cut(s) | 139, 142, 145 |
| Sau3AI | GATC | 3 cut(s) | 160, 193, 642 |
| Sau96I | GGNCC | 3 cut(s) | 154, 306, 440 |
| SchI | GAGTC | 1 cut(s) | 662 |
| ScrFI | CCNGG | 6 cut(s) | 107, 117, 153, 349, 384, 562 |
| SetI | ASST | 7 cut(s) | 129, 183, 220, 261, 343, 424, 641 |
| SfaNI | GCATC | 3 cut(s) | 390, 583, 609 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 398 |
| SspMI | CTAG | 4 cut(s) | 164, 182, 513, 629 |
| StyD4I | CCNGG | 6 cut(s) | 105, 115, 151, 347, 382, 560 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 2 cut(s) | 220, 343 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TauI | GCSGC | 2 cut(s) | 141, 147 |
| TfiI | GAWTC | 2 cut(s) | 482, 581 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 192, 417 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 1 cut(s) | 141 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspGWI | ACGGA | 1 cut(s) | 665 |
| TspRI | CASTG | 2 cut(s) | 192, 417 |
| Tth111I | GACNNNGTC | 1 cut(s) | 652 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XspI | CTAG | 4 cut(s) | 164, 182, 513, 629 |