No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 97 |
| AciI | CCGC | 5 cut(s) | 61, 97, 190, 208, 254 |
| AclI | AACGTT | 2 cut(s) | 104, 122 |
| AclWI | GGATC | 2 cut(s) | 180, 254 |
| AcoI | YGGCCR | 1 cut(s) | 347 |
| AcuI | CTGAAG | 1 cut(s) | 71 |
| AfaI | GTAC | 1 cut(s) | 177 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 162 |
| AgsI | TTSAA | 1 cut(s) | 307 |
| AjnI | CCWGG | 2 cut(s) | 219, 237 |
| AluBI | AGCT | 2 cut(s) | 267, 303 |
| AluI | AGCT | 2 cut(s) | 267, 303 |
| AlwI | GGATC | 2 cut(s) | 180, 254 |
| AoxI | GGCC | 3 cut(s) | 212, 332, 347 |
| ApeKI | GCWGC | 2 cut(s) | 205, 224 |
| AspS9I | GGNCC | 3 cut(s) | 63, 213, 333 |
| AsuHPI | GGTGA | 2 cut(s) | 63, 223 |
| AvaII | GGWCC | 1 cut(s) | 63 |
| AxyI | CCTNAGG | 1 cut(s) | 170 |
| BalI | TGGCCA | 1 cut(s) | 349 |
| BbvI | GCAGC | 2 cut(s) | 217, 236 |
| BccI | CCATC | 1 cut(s) | 11 |
| BciT130I | CCWGG | 2 cut(s) | 221, 239 |
| BfaI | CTAG | 2 cut(s) | 158, 250 |
| BfmI | CTRYAG | 1 cut(s) | 203 |
| BglII | AGATCT | 1 cut(s) | 279 |
| BisI | GCNGC | 4 cut(s) | 98, 206, 225, 254 |
| BlsI | GCNGC | 4 cut(s) | 99, 207, 226, 255 |
| Bme1390I | CCNGG | 2 cut(s) | 221, 239 |
| Bme18I | GGWCC | 1 cut(s) | 63 |
| BmgT120I | GGNCC | 3 cut(s) | 63, 213, 333 |
| BmiI | GGNNCC | 1 cut(s) | 167 |
| BmrFI | CCNGG | 2 cut(s) | 221, 239 |
| BmrI | ACTGGG | 1 cut(s) | 106 |
| BmuI | ACTGGG | 1 cut(s) | 106 |
| BsaWI | WCCGGW | 1 cut(s) | 147 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 162 |
| Bse1I | ACTGG | 1 cut(s) | 112 |
| Bse21I | CCTNAGG | 1 cut(s) | 170 |
| BseBI | CCWGG | 2 cut(s) | 221, 239 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 162 |
| BseMII | CTCAG | 1 cut(s) | 184 |
| BseNI | ACTGG | 1 cut(s) | 112 |
| BseXI | GCAGC | 2 cut(s) | 217, 236 |
| BsgI | GTGCAG | 1 cut(s) | 336 |
| Bsh1236I | CGCG | 1 cut(s) | 97 |
| BshFI | GGCC | 3 cut(s) | 214, 334, 349 |
| BsiSI | CCGG | 1 cut(s) | 148 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 162 |
| BsnI | GGCC | 3 cut(s) | 214, 334, 349 |
| Bsp143I | GATC | 3 cut(s) | 185, 246, 279 |
| BspACI | CCGC | 5 cut(s) | 61, 97, 190, 208, 254 |
| BspANI | GGCC | 3 cut(s) | 214, 334, 349 |
| BspCNI | CTCAG | 1 cut(s) | 183 |
| BspFNI | CGCG | 1 cut(s) | 97 |
| BspLI | GGNNCC | 1 cut(s) | 167 |
| BspMAI | CTGCAG | 1 cut(s) | 207 |
| BspPI | GGATC | 2 cut(s) | 180, 254 |
| BspQI | GCTCTTC | 1 cut(s) | 47 |
| BsrI | ACTGG | 1 cut(s) | 112 |
| BssMI | GATC | 3 cut(s) | 185, 246, 279 |
| Bst2UI | CCWGG | 2 cut(s) | 221, 239 |
| Bst4CI | ACNGT | 1 cut(s) | 130 |
| Bst6I | CTCTTC | 1 cut(s) | 47 |
| BstC8I | GCNNGC | 2 cut(s) | 59, 269 |
| BstDEI | CTNAG | 1 cut(s) | 170 |
| BstFNI | CGCG | 1 cut(s) | 97 |
| BstKTI | GATC | 3 cut(s) | 188, 249, 282 |
| BstMBI | GATC | 3 cut(s) | 185, 246, 279 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 211 |
| BstNI | CCWGG | 2 cut(s) | 221, 239 |
| BstSCI | CCNGG | 2 cut(s) | 219, 237 |
| BstSFI | CTRYAG | 1 cut(s) | 203 |
| BstUI | CGCG | 1 cut(s) | 97 |
| BstV1I | GCAGC | 2 cut(s) | 217, 236 |
| BstX2I | RGATCY | 3 cut(s) | 185, 246, 279 |
| BstYI | RGATCY | 3 cut(s) | 185, 246, 279 |
| Bsu36I | CCTNAGG | 1 cut(s) | 170 |
| BsuRI | GGCC | 3 cut(s) | 214, 334, 349 |
| BtsIMutI | CAGTG | 1 cut(s) | 119 |
| Cac8I | GCNNGC | 2 cut(s) | 59, 269 |
| Cfr13I | GGNCC | 3 cut(s) | 63, 213, 333 |
| CsiI | ACCWGGT | 1 cut(s) | 237 |
| Csp6I | GTAC | 1 cut(s) | 176 |
| CviJI | RGCY | 7 cut(s) | 100, 214, 253, 267, 303, 334, 349 |
| CviKI_1 | RGCY | 7 cut(s) | 100, 214, 253, 267, 303, 334, 349 |
| CviQI | GTAC | 1 cut(s) | 176 |
| DdeI | CTNAG | 1 cut(s) | 170 |
| DpnI | GATC | 3 cut(s) | 187, 248, 281 |
| DpnII | GATC | 3 cut(s) | 185, 246, 279 |
| EaeI | YGGCCR | 1 cut(s) | 347 |
| Eam1104I | CTCTTC | 1 cut(s) | 47 |
| EarI | CTCTTC | 1 cut(s) | 47 |
| EciI | GGCGGA | 1 cut(s) | 76 |
| Eco47I | GGWCC | 1 cut(s) | 63 |
| Eco57I | CTGAAG | 1 cut(s) | 71 |
| Eco81I | CCTNAGG | 1 cut(s) | 170 |
| EcoRII | CCWGG | 2 cut(s) | 219, 237 |
| FaiI | YATR | 4 cut(s) | 25, 259, 263, 287 |
| FauI | CCCGC | 1 cut(s) | 197 |
| Fnu4HI | GCNGC | 4 cut(s) | 98, 206, 225, 254 |
| Fsp4HI | GCNGC | 4 cut(s) | 98, 206, 225, 254 |
| FspBI | CTAG | 2 cut(s) | 158, 250 |
| GluI | GCNGC | 4 cut(s) | 98, 206, 225, 254 |
| HaeIII | GGCC | 3 cut(s) | 214, 334, 349 |
| HapII | CCGG | 1 cut(s) | 148 |
| HincII | GTYRAC | 3 cut(s) | 108, 126, 153 |
| HindII | GTYRAC | 3 cut(s) | 108, 126, 153 |
| HinfI | GANTC | 2 cut(s) | 195, 365 |
| HpaI | GTTAAC | 2 cut(s) | 108, 126 |
| HpaII | CCGG | 1 cut(s) | 148 |
| HphI | GGTGA | 2 cut(s) | 63, 223 |
| Hpy166II | GTNNAC | 4 cut(s) | 108, 126, 153, 178 |
| Hpy188III | TCNNGA | 2 cut(s) | 244, 362 |
| Hpy8I | GTNNAC | 4 cut(s) | 108, 126, 153, 178 |
| HpyCH4III | ACNGT | 1 cut(s) | 130 |
| HpyCH4IV | ACGT | 3 cut(s) | 104, 122, 352 |
| HpyCH4V | TGCA | 2 cut(s) | 205, 317 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 211 |
| HpyF3I | CTNAG | 1 cut(s) | 170 |
| HpySE526I | ACGT | 3 cut(s) | 104, 122, 352 |
| KspAI | GTTAAC | 2 cut(s) | 108, 126 |
| Kzo9I | GATC | 3 cut(s) | 185, 246, 279 |
| LguI | GCTCTTC | 1 cut(s) | 47 |
| LmnI | GCTCC | 1 cut(s) | 300 |
| Lsp1109I | GCAGC | 2 cut(s) | 217, 236 |
| MabI | ACCWGGT | 1 cut(s) | 237 |
| MaeI | CTAG | 2 cut(s) | 158, 250 |
| MaeII | ACGT | 3 cut(s) | 104, 122, 352 |
| MaeIII | GTNAC | 2 cut(s) | 118, 229 |
| MalI | GATC | 3 cut(s) | 187, 248, 281 |
| MboI | GATC | 3 cut(s) | 185, 246, 279 |
| MboII | GAAGA | 1 cut(s) | 64 |
| MflI | RGATCY | 3 cut(s) | 185, 246, 279 |
| MlsI | TGGCCA | 1 cut(s) | 349 |
| MluNI | TGGCCA | 1 cut(s) | 349 |
| MlyI | GAGTC | 1 cut(s) | 189 |
| MnlI | CCTC | 5 cut(s) | 31, 103, 179, 204, 324 |
| Mox20I | TGGCCA | 1 cut(s) | 349 |
| MscI | TGGCCA | 1 cut(s) | 349 |
| MseI | TTAA | 2 cut(s) | 107, 125 |
| MslI | CAYNNNNRTG | 1 cut(s) | 355 |
| Msp20I | TGGCCA | 1 cut(s) | 349 |
| MspA1I | CMGCKG | 2 cut(s) | 192, 208 |
| MspI | CCGG | 1 cut(s) | 148 |
| MspR9I | CCNGG | 2 cut(s) | 221, 239 |
| MvaI | CCWGG | 2 cut(s) | 221, 239 |
| MvnI | CGCG | 1 cut(s) | 97 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 211 |
| NdeII | GATC | 3 cut(s) | 185, 246, 279 |
| NlaIV | GGNNCC | 1 cut(s) | 167 |
| NmuCI | GTSAC | 1 cut(s) | 229 |
| PciSI | GCTCTTC | 1 cut(s) | 47 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 101 |
| PfeI | GAWTC | 1 cut(s) | 365 |
| PkrI | GCNGC | 4 cut(s) | 99, 207, 226, 255 |
| PleI | GAGTC | 1 cut(s) | 189 |
| PpsI | GAGTC | 1 cut(s) | 189 |
| Psp1406I | AACGTT | 2 cut(s) | 104, 122 |
| Psp6I | CCWGG | 2 cut(s) | 219, 237 |
| PspGI | CCWGG | 2 cut(s) | 219, 237 |
| PspN4I | GGNNCC | 1 cut(s) | 167 |
| PspPI | GGNCC | 3 cut(s) | 63, 213, 333 |
| PstI | CTGCAG | 1 cut(s) | 207 |
| PsuI | RGATCY | 3 cut(s) | 185, 246, 279 |
| RsaI | GTAC | 1 cut(s) | 177 |
| RsaNI | GTAC | 1 cut(s) | 176 |
| RseI | CAYNNNNRTG | 1 cut(s) | 355 |
| SapI | GCTCTTC | 1 cut(s) | 47 |
| SaqAI | TTAA | 2 cut(s) | 107, 125 |
| SatI | GCNGC | 4 cut(s) | 98, 206, 225, 254 |
| Sau3AI | GATC | 3 cut(s) | 185, 246, 279 |
| Sau96I | GGNCC | 3 cut(s) | 63, 213, 333 |
| SchI | GAGTC | 1 cut(s) | 189 |
| ScrFI | CCNGG | 2 cut(s) | 221, 239 |
| SetI | ASST | 9 cut(s) | 95, 107, 125, 171, 243, 269, 305, 329, 355 |
| SexAI | ACCWGGT | 1 cut(s) | 237 |
| SfcI | CTRYAG | 1 cut(s) | 203 |
| SinI | GGWCC | 1 cut(s) | 63 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 355 |
| SsiI | CCGC | 5 cut(s) | 61, 97, 190, 208, 254 |
| SspMI | CTAG | 2 cut(s) | 158, 250 |
| StyD4I | CCNGG | 2 cut(s) | 219, 237 |
| TaaI | ACNGT | 1 cut(s) | 130 |
| TaiI | ACGT | 3 cut(s) | 107, 125, 355 |
| TaqI | TCGA | 2 cut(s) | 329, 368 |
| TauI | GCSGC | 2 cut(s) | 100, 256 |
| TfiI | GAWTC | 1 cut(s) | 365 |
| Tru1I | TTAA | 2 cut(s) | 107, 125 |
| Tru9I | TTAA | 2 cut(s) | 107, 125 |
| TscAI | CASTG | 1 cut(s) | 119 |
| TseFI | GTSAC | 1 cut(s) | 229 |
| TseI | GCWGC | 2 cut(s) | 205, 224 |
| Tsp45I | GTSAC | 1 cut(s) | 229 |
| TspRI | CASTG | 1 cut(s) | 119 |
| VpaK11BI | GGWCC | 1 cut(s) | 63 |
| XspI | CTAG | 2 cut(s) | 158, 250 |