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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 30 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 334 |
| AccBSI | CCGCTC | 1 cut(s) | 242 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AccII | CGCG | 1 cut(s) | 106 |
| AciI | CCGC | 8 cut(s) | 20, 217, 229, 240, 274, 289, 324, 374 |
| AclWI | GGATC | 2 cut(s) | 30, 361 |
| AcuI | CTGAAG | 1 cut(s) | 284 |
| AfaI | GTAC | 1 cut(s) | 5 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 197, 334 |
| AjnI | CCWGG | 1 cut(s) | 176 |
| AluBI | AGCT | 3 cut(s) | 10, 137, 250 |
| AluI | AGCT | 3 cut(s) | 10, 137, 250 |
| AlwI | GGATC | 2 cut(s) | 30, 361 |
| AoxI | GGCC | 1 cut(s) | 75 |
| AspLEI | GCGC | 3 cut(s) | 154, 203, 282 |
| AspS9I | GGNCC | 1 cut(s) | 76 |
| AsuC2I | CCSGG | 2 cut(s) | 192, 371 |
| AsuNHI | GCTAGC | 1 cut(s) | 10 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BciT130I | CCWGG | 1 cut(s) | 178 |
| BcnI | CCSGG | 2 cut(s) | 192, 371 |
| BfaI | CTAG | 3 cut(s) | 11, 251, 353 |
| BisI | GCNGC | 1 cut(s) | 229 |
| BlsI | GCNGC | 1 cut(s) | 230 |
| Bme1390I | CCNGG | 3 cut(s) | 178, 192, 371 |
| BmgT120I | GGNCC | 1 cut(s) | 76 |
| BmrFI | CCNGG | 3 cut(s) | 178, 192, 371 |
| BmsI | GCATC | 2 cut(s) | 307, 333 |
| BmtI | GCTAGC | 1 cut(s) | 14 |
| BpmI | CTGGAG | 1 cut(s) | 269 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| BpuMI | CCSGG | 2 cut(s) | 192, 371 |
| BsaAI | YACGTR | 1 cut(s) | 170 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 197, 334 |
| Bse118I | RCCGGY | 1 cut(s) | 311 |
| Bse1I | ACTGG | 2 cut(s) | 21, 202 |
| BseBI | CCWGG | 1 cut(s) | 178 |
| BseGI | GGATG | 1 cut(s) | 348 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 197, 334 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseNI | ACTGG | 2 cut(s) | 21, 202 |
| BseRI | GAGGAG | 1 cut(s) | 65 |
| Bsh1236I | CGCG | 1 cut(s) | 106 |
| BshFI | GGCC | 1 cut(s) | 77 |
| BsiSI | CCGG | 3 cut(s) | 192, 312, 370 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 197, 334 |
| BsnI | GGCC | 1 cut(s) | 77 |
| Bsp143I | GATC | 2 cut(s) | 22, 366 |
| BspACI | CCGC | 8 cut(s) | 20, 217, 229, 240, 274, 289, 324, 374 |
| BspANI | GGCC | 1 cut(s) | 77 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspFNI | CGCG | 1 cut(s) | 106 |
| BspOI | GCTAGC | 1 cut(s) | 14 |
| BspPI | GGATC | 2 cut(s) | 30, 361 |
| BsrBI | CCGCTC | 1 cut(s) | 242 |
| BsrFI | RCCGGY | 1 cut(s) | 311 |
| BsrI | ACTGG | 2 cut(s) | 21, 202 |
| BssAI | RCCGGY | 1 cut(s) | 311 |
| BssMI | GATC | 2 cut(s) | 22, 366 |
| Bst2UI | CCWGG | 1 cut(s) | 178 |
| Bst4CI | ACNGT | 1 cut(s) | 87 |
| BstBAI | YACGTR | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 4 cut(s) | 12, 278, 300, 313 |
| BstDEI | CTNAG | 1 cut(s) | 72 |
| BstF5I | GGATG | 1 cut(s) | 348 |
| BstFNI | CGCG | 1 cut(s) | 106 |
| BstHHI | GCGC | 3 cut(s) | 154, 203, 282 |
| BstKTI | GATC | 2 cut(s) | 25, 369 |
| BstMBI | GATC | 2 cut(s) | 22, 366 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 103, 286, 295, 308, 317 |
| BstNI | CCWGG | 1 cut(s) | 178 |
| BstSCI | CCNGG | 3 cut(s) | 176, 190, 369 |
| BstUI | CGCG | 1 cut(s) | 106 |
| BstX2I | RGATCY | 1 cut(s) | 22 |
| BstYI | RGATCY | 1 cut(s) | 22 |
| BsuRI | GGCC | 1 cut(s) | 77 |
| BtgZI | GCGATG | 1 cut(s) | 330 |
| BtsCI | GGATG | 1 cut(s) | 348 |
| BtsI | GCAGTG | 1 cut(s) | 223 |
| BtsIMutI | CAGTG | 3 cut(s) | 14, 195, 223 |
| Cac8I | GCNNGC | 4 cut(s) | 12, 278, 300, 313 |
| CfoI | GCGC | 3 cut(s) | 154, 203, 282 |
| Cfr10I | RCCGGY | 1 cut(s) | 311 |
| Cfr13I | GGNCC | 1 cut(s) | 76 |
| CseI | GACGC | 1 cut(s) | 136 |
| Csp6I | GTAC | 1 cut(s) | 4 |
| CviJI | RGCY | 7 cut(s) | 10, 71, 77, 92, 137, 250, 302 |
| CviKI_1 | RGCY | 7 cut(s) | 10, 71, 77, 92, 137, 250, 302 |
| CviQI | GTAC | 1 cut(s) | 4 |
| DdeI | CTNAG | 1 cut(s) | 72 |
| DpnI | GATC | 2 cut(s) | 24, 368 |
| DpnII | GATC | 2 cut(s) | 22, 366 |
| EciI | GGCGGA | 2 cut(s) | 35, 263 |
| Eco57I | CTGAAG | 1 cut(s) | 284 |
| EcoRII | CCWGG | 1 cut(s) | 176 |
| FaiI | YATR | 2 cut(s) | 30, 296 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 1 cut(s) | 229 |
| FokI | GGATG | 1 cut(s) | 355 |
| Fsp4HI | GCNGC | 1 cut(s) | 229 |
| FspBI | CTAG | 3 cut(s) | 11, 251, 353 |
| GlaI | GCGC | 3 cut(s) | 153, 202, 281 |
| GluI | GCNGC | 1 cut(s) | 229 |
| GsuI | CTGGAG | 1 cut(s) | 269 |
| HaeIII | GGCC | 1 cut(s) | 77 |
| HapII | CCGG | 3 cut(s) | 192, 312, 370 |
| HgaI | GACGC | 1 cut(s) | 136 |
| HhaI | GCGC | 3 cut(s) | 154, 203, 282 |
| Hin6I | GCGC | 3 cut(s) | 152, 201, 280 |
| HinP1I | GCGC | 3 cut(s) | 152, 201, 280 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 2 cut(s) | 220, 377 |
| HpaII | CCGG | 3 cut(s) | 192, 312, 370 |
| Hpy166II | GTNNAC | 1 cut(s) | 188 |
| Hpy188I | TCNGA | 1 cut(s) | 246 |
| Hpy8I | GTNNAC | 1 cut(s) | 188 |
| HpyAV | CCTTC | 2 cut(s) | 61, 259 |
| HpyCH4III | ACNGT | 1 cut(s) | 87 |
| HpyCH4IV | ACGT | 2 cut(s) | 46, 169 |
| HpyCH4V | TGCA | 3 cut(s) | 298, 320, 346 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 103, 286, 295, 308, 317 |
| HpyF3I | CTNAG | 1 cut(s) | 72 |
| HpySE526I | ACGT | 2 cut(s) | 46, 169 |
| HspAI | GCGC | 3 cut(s) | 152, 201, 280 |
| KroI | GCCGGC | 1 cut(s) | 311 |
| KroNI | GCCGGC | 1 cut(s) | 313 |
| Kzo9I | GATC | 2 cut(s) | 22, 366 |
| LweI | GCATC | 2 cut(s) | 307, 333 |
| MaeI | CTAG | 3 cut(s) | 11, 251, 353 |
| MaeII | ACGT | 2 cut(s) | 46, 169 |
| MaeIII | GTNAC | 1 cut(s) | 170 |
| MalI | GATC | 2 cut(s) | 24, 368 |
| MbiI | CCGCTC | 1 cut(s) | 242 |
| MboI | GATC | 2 cut(s) | 22, 366 |
| MflI | RGATCY | 1 cut(s) | 22 |
| MnlI | CCTC | 7 cut(s) | 43, 46, 67, 151, 251, 293, 354 |
| MreI | CGCCGGCG | 1 cut(s) | 311 |
| MroNI | GCCGGC | 1 cut(s) | 311 |
| MspA1I | CMGCKG | 1 cut(s) | 289 |
| MspI | CCGG | 3 cut(s) | 192, 312, 370 |
| MspR9I | CCNGG | 3 cut(s) | 178, 192, 371 |
| MvaI | CCWGG | 1 cut(s) | 178 |
| MvnI | CGCG | 1 cut(s) | 106 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 103, 286, 295, 308, 317 |
| NaeI | GCCGGC | 1 cut(s) | 313 |
| NciI | CCSGG | 2 cut(s) | 192, 371 |
| NdeII | GATC | 2 cut(s) | 22, 366 |
| NgoMIV | GCCGGC | 1 cut(s) | 311 |
| NheI | GCTAGC | 1 cut(s) | 10 |
| PdiI | GCCGGC | 1 cut(s) | 313 |
| PfeI | GAWTC | 1 cut(s) | 220 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 334 |
| PkrI | GCNGC | 1 cut(s) | 230 |
| Ppu21I | YACGTR | 1 cut(s) | 170 |
| PsiI | TTATAA | 1 cut(s) | 30 |
| Psp6I | CCWGG | 1 cut(s) | 176 |
| PspGI | CCWGG | 1 cut(s) | 176 |
| PspPI | GGNCC | 1 cut(s) | 76 |
| PsuI | RGATCY | 1 cut(s) | 22 |
| RsaI | GTAC | 1 cut(s) | 5 |
| RsaNI | GTAC | 1 cut(s) | 4 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SatI | GCNGC | 1 cut(s) | 229 |
| Sau3AI | GATC | 2 cut(s) | 22, 366 |
| Sau96I | GGNCC | 1 cut(s) | 76 |
| ScrFI | CCNGG | 3 cut(s) | 178, 192, 371 |
| SetI | ASST | 7 cut(s) | 12, 49, 139, 143, 172, 252, 365 |
| SfaNI | GCATC | 2 cut(s) | 307, 333 |
| SgrAI | CRCCGGYG | 1 cut(s) | 311 |
| SsiI | CCGC | 8 cut(s) | 20, 217, 229, 240, 274, 289, 324, 374 |
| SspMI | CTAG | 3 cut(s) | 11, 251, 353 |
| StyD4I | CCNGG | 3 cut(s) | 176, 190, 369 |
| TaaI | ACNGT | 1 cut(s) | 87 |
| TaiI | ACGT | 2 cut(s) | 49, 172 |
| TaqI | TCGA | 1 cut(s) | 187 |
| TauI | GCSGC | 1 cut(s) | 231 |
| TfiI | GAWTC | 1 cut(s) | 220 |
| TscAI | CASTG | 3 cut(s) | 21, 202, 230 |
| TspDTI | ATGAA | 1 cut(s) | 129 |
| TspRI | CASTG | 3 cut(s) | 21, 202, 230 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 334 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 3 cut(s) | 11, 251, 353 |