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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 394 |
| Acc65I | GGTACC | 1 cut(s) | 379 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 659 |
| AccBSI | CCGCTC | 3 cut(s) | 504, 602, 650 |
| AccII | CGCG | 3 cut(s) | 90, 219, 692 |
| AclWI | GGATC | 2 cut(s) | 562, 782 |
| AcoI | YGGCCR | 2 cut(s) | 261, 729 |
| AfaI | GTAC | 3 cut(s) | 324, 381, 746 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 437, 551 |
| AgeI | ACCGGT | 1 cut(s) | 739 |
| AgsI | TTSAA | 3 cut(s) | 563, 678, 814 |
| AjnI | CCWGG | 6 cut(s) | 151, 347, 430, 545, 695, 700 |
| AleI | CACNNNNGTG | 1 cut(s) | 269 |
| AluBI | AGCT | 3 cut(s) | 181, 289, 427 |
| AluI | AGCT | 3 cut(s) | 181, 289, 427 |
| Alw21I | GWGCWC | 1 cut(s) | 347 |
| Alw26I | GTCTC | 3 cut(s) | 119, 153, 502 |
| AlwI | GGATC | 2 cut(s) | 562, 782 |
| Ama87I | CYCGRG | 1 cut(s) | 505 |
| AoxI | GGCC | 7 cut(s) | 154, 240, 246, 261, 440, 698, 729 |
| ApeKI | GCWGC | 3 cut(s) | 141, 412, 622 |
| AsiGI | ACCGGT | 1 cut(s) | 739 |
| Asp718I | GGTACC | 1 cut(s) | 379 |
| AspA2I | CCTAGG | 1 cut(s) | 512 |
| AspLEI | GCGC | 4 cut(s) | 219, 374, 417, 670 |
| AspS9I | GGNCC | 5 cut(s) | 193, 247, 406, 440, 581 |
| AsuC2I | CCSGG | 4 cut(s) | 107, 117, 320, 574 |
| AsuHPI | GGTGA | 3 cut(s) | 91, 134, 814 |
| AvaI | CYCGRG | 1 cut(s) | 505 |
| AvaII | GGWCC | 3 cut(s) | 193, 406, 581 |
| AvrII | CCTAGG | 1 cut(s) | 512 |
| BaeGI | GKGCMC | 1 cut(s) | 456 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 728, 761 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanI | GGYRCC | 2 cut(s) | 379, 659 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| Bbv12I | GWGCWC | 1 cut(s) | 347 |
| BbvI | GCAGC | 3 cut(s) | 153, 399, 634 |
| BccI | CCATC | 2 cut(s) | 71, 465 |
| BceAI | ACGGC | 1 cut(s) | 716 |
| BciT130I | CCWGG | 6 cut(s) | 153, 349, 432, 547, 697, 702 |
| BcnI | CCSGG | 4 cut(s) | 107, 117, 320, 574 |
| BcoDI | GTCTC | 3 cut(s) | 119, 153, 502 |
| BfaI | CTAG | 4 cut(s) | 188, 513, 578, 611 |
| BfoI | RGCGCY | 1 cut(s) | 671 |
| BfuAI | ACCTGC | 1 cut(s) | 394 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 5 cut(s) | 142, 145, 413, 443, 623 |
| BlnI | CCTAGG | 1 cut(s) | 512 |
| BlsI | GCNGC | 5 cut(s) | 143, 146, 414, 444, 624 |
| Bme18I | GGWCC | 3 cut(s) | 193, 406, 581 |
| BmeT110I | CYCGRG | 1 cut(s) | 505 |
| BmgT120I | GGNCC | 5 cut(s) | 193, 247, 406, 440, 581 |
| BmiI | GGNNCC | 4 cut(s) | 381, 408, 583, 661 |
| BmsI | GCATC | 3 cut(s) | 90, 583, 609 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 4 cut(s) | 107, 117, 320, 574 |
| BsaI | GGTCTC | 2 cut(s) | 153, 502 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 115, 243, 512, 525, 545 |
| BsaWI | WCCGGW | 1 cut(s) | 739 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 368, 437, 551 |
| Bse118I | RCCGGY | 3 cut(s) | 368, 587, 739 |
| Bse1I | ACTGG | 1 cut(s) | 389 |
| BseBI | CCWGG | 6 cut(s) | 153, 349, 432, 547, 697, 702 |
| BseDI | CCNNGG | 6 cut(s) | 105, 115, 243, 512, 525, 545 |
| BseGI | GGATG | 1 cut(s) | 624 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 437, 551 |
| BseMII | CTCAG | 1 cut(s) | 617 |
| BseNI | ACTGG | 1 cut(s) | 389 |
| BseSI | GKGCMC | 1 cut(s) | 456 |
| BseXI | GCAGC | 3 cut(s) | 153, 399, 634 |
| BseYI | CCCAGC | 2 cut(s) | 70, 664 |
| BsgI | GTGCAG | 1 cut(s) | 476 |
| Bsh1236I | CGCG | 3 cut(s) | 90, 219, 692 |
| BshFI | GGCC | 7 cut(s) | 156, 242, 248, 263, 442, 700, 731 |
| BshNI | GGYRCC | 2 cut(s) | 379, 659 |
| BshTI | ACCGGT | 1 cut(s) | 739 |
| BsiHKAI | GWGCWC | 1 cut(s) | 347 |
| BsiHKCI | CYCGRG | 1 cut(s) | 505 |
| BsiSI | CCGG | 7 cut(s) | 107, 117, 320, 369, 574, 588, 740 |
| BslFI | GGGAC | 2 cut(s) | 392, 567 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 437, 551 |
| BsmAI | GTCTC | 3 cut(s) | 119, 153, 502 |
| BsmFI | GGGAC | 2 cut(s) | 392, 567 |
| BsnI | GGCC | 7 cut(s) | 156, 242, 248, 263, 442, 700, 731 |
| Bso31I | GGTCTC | 2 cut(s) | 153, 502 |
| BsoBI | CYCGRG | 1 cut(s) | 505 |
| Bsp1286I | GDGCHC | 2 cut(s) | 347, 456 |
| Bsp143I | GATC | 2 cut(s) | 554, 787 |
| BspANI | GGCC | 7 cut(s) | 156, 242, 248, 263, 442, 700, 731 |
| BspCNI | CTCAG | 1 cut(s) | 616 |
| BspFNI | CGCG | 3 cut(s) | 90, 219, 692 |
| BspLI | GGNNCC | 4 cut(s) | 381, 408, 583, 661 |
| BspMI | ACCTGC | 1 cut(s) | 394 |
| BspPI | GGATC | 2 cut(s) | 562, 782 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 659 |
| BspTNI | GGTCTC | 2 cut(s) | 153, 502 |
| BsrBI | CCGCTC | 3 cut(s) | 504, 602, 650 |
| BsrFI | RCCGGY | 3 cut(s) | 368, 587, 739 |
| BsrI | ACTGG | 1 cut(s) | 389 |
| BssAI | RCCGGY | 3 cut(s) | 368, 587, 739 |
| BssECI | CCNNGG | 6 cut(s) | 105, 115, 243, 512, 525, 545 |
| BssMI | GATC | 2 cut(s) | 554, 787 |
| BssT1I | CCWWGG | 1 cut(s) | 512 |
| Bst2UI | CCWGG | 6 cut(s) | 153, 349, 432, 547, 697, 702 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 45, 336 |
| BstC8I | GCNNGC | 8 cut(s) | 28, 32, 36, 92, 149, 183, 589, 729 |
| BstDEI | CTNAG | 1 cut(s) | 603 |
| BstEII | GGTNACC | 1 cut(s) | 820 |
| BstF5I | GGATG | 1 cut(s) | 624 |
| BstFNI | CGCG | 3 cut(s) | 90, 219, 692 |
| BstH2I | RGCGCY | 1 cut(s) | 671 |
| BstHHI | GCGC | 4 cut(s) | 219, 374, 417, 670 |
| BstKTI | GATC | 2 cut(s) | 557, 790 |
| BstMAI | GTCTC | 3 cut(s) | 119, 153, 502 |
| BstMBI | GATC | 2 cut(s) | 554, 787 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 87, 153, 409, 451, 593 |
| BstNI | CCWGG | 6 cut(s) | 153, 349, 432, 547, 697, 702 |
| BstPI | GGTNACC | 1 cut(s) | 820 |
| BstSLI | GKGCMC | 1 cut(s) | 456 |
| BstUI | CGCG | 3 cut(s) | 90, 219, 692 |
| BstV1I | GCAGC | 3 cut(s) | 153, 399, 634 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BsuRI | GGCC | 7 cut(s) | 156, 242, 248, 263, 442, 700, 731 |
| BtgZI | GCGATG | 1 cut(s) | 606 |
| BtsCI | GGATG | 1 cut(s) | 624 |
| BtsI | GCAGTG | 1 cut(s) | 488 |
| BtsIMutI | CAGTG | 1 cut(s) | 488 |
| BveI | ACCTGC | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 8 cut(s) | 28, 32, 36, 92, 149, 183, 589, 729 |
| CfoI | GCGC | 4 cut(s) | 219, 374, 417, 670 |
| Cfr10I | RCCGGY | 3 cut(s) | 368, 587, 739 |
| Cfr13I | GGNCC | 5 cut(s) | 193, 247, 406, 440, 581 |
| CseI | GACGC | 2 cut(s) | 32, 681 |
| Csp6I | GTAC | 3 cut(s) | 323, 380, 745 |
| CspAI | ACCGGT | 1 cut(s) | 739 |
| CviAII | CATG | 1 cut(s) | 802 |
| CviQI | GTAC | 3 cut(s) | 323, 380, 745 |
| DdeI | CTNAG | 1 cut(s) | 603 |
| DpnI | GATC | 2 cut(s) | 556, 789 |
| DpnII | GATC | 2 cut(s) | 554, 787 |
| EaeI | YGGCCR | 2 cut(s) | 261, 729 |
| EciI | GGCGGA | 1 cut(s) | 37 |
| Eco130I | CCWWGG | 1 cut(s) | 512 |
| Eco31I | GGTCTC | 2 cut(s) | 153, 502 |
| Eco47I | GGWCC | 3 cut(s) | 193, 406, 581 |
| Eco88I | CYCGRG | 1 cut(s) | 505 |
| Eco91I | GGTNACC | 1 cut(s) | 820 |
| EcoO109I | RGGNCCY | 2 cut(s) | 406, 581 |
| EcoO65I | GGTNACC | 1 cut(s) | 820 |
| EcoRII | CCWGG | 6 cut(s) | 151, 347, 430, 545, 695, 700 |
| EcoT14I | CCWWGG | 1 cut(s) | 512 |
| ErhI | CCWWGG | 1 cut(s) | 512 |
| FaeI | CATG | 1 cut(s) | 805 |
| FaiI | YATR | 7 cut(s) | 53, 175, 177, 399, 401, 774, 803 |
| FaqI | GGGAC | 2 cut(s) | 392, 567 |
| FatI | CATG | 1 cut(s) | 801 |
| FauI | CCCGC | 4 cut(s) | 8, 156, 417, 592 |
| FauNDI | CATATG | 1 cut(s) | 399 |
| Fnu4HI | GCNGC | 5 cut(s) | 142, 145, 413, 443, 623 |
| FokI | GGATG | 1 cut(s) | 631 |
| Fsp4HI | GCNGC | 5 cut(s) | 142, 145, 413, 443, 623 |
| FspBI | CTAG | 4 cut(s) | 188, 513, 578, 611 |
| GlaI | GCGC | 4 cut(s) | 218, 373, 416, 669 |
| GluI | GCNGC | 5 cut(s) | 142, 145, 413, 443, 623 |
| GsaI | CCCAGC | 2 cut(s) | 74, 668 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 671 |
| HaeIII | GGCC | 7 cut(s) | 156, 242, 248, 263, 442, 700, 731 |
| HapII | CCGG | 7 cut(s) | 107, 117, 320, 369, 574, 588, 740 |
| HgaI | GACGC | 2 cut(s) | 32, 681 |
| HhaI | GCGC | 4 cut(s) | 219, 374, 417, 670 |
| Hin1II | CATG | 1 cut(s) | 805 |
| Hin6I | GCGC | 4 cut(s) | 217, 372, 415, 668 |
| HinP1I | GCGC | 4 cut(s) | 217, 372, 415, 668 |
| HinfI | GANTC | 4 cut(s) | 435, 482, 653, 754 |
| HpaII | CCGG | 7 cut(s) | 107, 117, 320, 369, 574, 588, 740 |
| HphI | GGTGA | 3 cut(s) | 91, 134, 814 |
| Hpy166II | GTNNAC | 2 cut(s) | 745, 799 |
| Hpy188I | TCNGA | 3 cut(s) | 606, 647, 737 |
| Hpy188III | TCNNGA | 2 cut(s) | 507, 814 |
| Hpy8I | GTNNAC | 2 cut(s) | 745, 799 |
| HpyAV | CCTTC | 5 cut(s) | 206, 549, 802, 811, 820 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 45, 336 |
| HpyCH4V | TGCA | 5 cut(s) | 397, 403, 493, 596, 622 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 87, 153, 409, 451, 593 |
| HpyF3I | CTNAG | 1 cut(s) | 603 |
| Hsp92II | CATG | 1 cut(s) | 805 |
| HspAI | GCGC | 4 cut(s) | 217, 372, 415, 668 |
| KpnI | GGTACC | 1 cut(s) | 383 |
| KroI | GCCGGC | 1 cut(s) | 587 |
| KroNI | GCCGGC | 1 cut(s) | 589 |
| Kzo9I | GATC | 2 cut(s) | 554, 787 |
| LmnI | GCTCC | 1 cut(s) | 342 |
| Lsp1109I | GCAGC | 3 cut(s) | 153, 399, 634 |
| LweI | GCATC | 3 cut(s) | 90, 583, 609 |
| MaeI | CTAG | 4 cut(s) | 188, 513, 578, 611 |
| MaeIII | GTNAC | 4 cut(s) | 273, 336, 765, 820 |
| MalI | GATC | 2 cut(s) | 556, 789 |
| MbiI | CCGCTC | 3 cut(s) | 504, 602, 650 |
| MboI | GATC | 2 cut(s) | 554, 787 |
| MboII | GAAGA | 1 cut(s) | 32 |
| MhlI | GDGCHC | 2 cut(s) | 347, 456 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 2 cut(s) | 672, 720 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MlyI | GAGTC | 2 cut(s) | 491, 748 |
| MmeI | TCCRAC | 2 cut(s) | 447, 609 |
| MnlI | CCTC | 7 cut(s) | 173, 178, 238, 298, 469, 520, 545 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MreI | CGCCGGCG | 1 cut(s) | 587 |
| MroNI | GCCGGC | 1 cut(s) | 587 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 1 cut(s) | 641 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 412, 716 |
| MspI | CCGG | 7 cut(s) | 107, 117, 320, 369, 574, 588, 740 |
| MvaI | CCWGG | 6 cut(s) | 153, 349, 432, 547, 697, 702 |
| MvnI | CGCG | 3 cut(s) | 90, 219, 692 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 87, 153, 409, 451, 593 |
| NaeI | GCCGGC | 1 cut(s) | 589 |
| NciI | CCSGG | 4 cut(s) | 107, 117, 320, 574 |
| NdeI | CATATG | 1 cut(s) | 399 |
| NdeII | GATC | 2 cut(s) | 554, 787 |
| NgoMIV | GCCGGC | 1 cut(s) | 587 |
| NlaIII | CATG | 1 cut(s) | 805 |
| NlaIV | GGNNCC | 4 cut(s) | 381, 408, 583, 661 |
| NmeAIII | GCCGAG | 2 cut(s) | 182, 268 |
| NmuCI | GTSAC | 4 cut(s) | 273, 336, 765, 820 |
| OliI | CACNNNNGTG | 1 cut(s) | 269 |
| PaeR7I | CTCGAG | 1 cut(s) | 505 |
| PdiI | GCCGGC | 1 cut(s) | 589 |
| PfeI | GAWTC | 2 cut(s) | 435, 653 |
| PinAI | ACCGGT | 1 cut(s) | 739 |
| PkrI | GCNGC | 5 cut(s) | 143, 146, 414, 444, 624 |
| PleI | GAGTC | 2 cut(s) | 490, 748 |
| PpsI | GAGTC | 2 cut(s) | 490, 748 |
| PpuMI | RGGWCCY | 2 cut(s) | 406, 581 |
| Psp5II | RGGWCCY | 2 cut(s) | 406, 581 |
| Psp6I | CCWGG | 6 cut(s) | 151, 347, 430, 545, 695, 700 |
| PspEI | GGTNACC | 1 cut(s) | 820 |
| PspFI | CCCAGC | 2 cut(s) | 70, 664 |
| PspGI | CCWGG | 6 cut(s) | 151, 347, 430, 545, 695, 700 |
| PspN4I | GGNNCC | 4 cut(s) | 381, 408, 583, 661 |
| PspPI | GGNCC | 5 cut(s) | 193, 247, 406, 440, 581 |
| PspPPI | RGGWCCY | 2 cut(s) | 406, 581 |
| RsaI | GTAC | 3 cut(s) | 324, 381, 746 |
| RsaNI | GTAC | 3 cut(s) | 323, 380, 745 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SaqAI | TTAA | 1 cut(s) | 641 |
| SatI | GCNGC | 5 cut(s) | 142, 145, 413, 443, 623 |
| Sau3AI | GATC | 2 cut(s) | 554, 787 |
| Sau96I | GGNCC | 5 cut(s) | 193, 247, 406, 440, 581 |
| SchI | GAGTC | 2 cut(s) | 491, 748 |
| SduI | GDGCHC | 2 cut(s) | 347, 456 |
| SfaNI | GCATC | 3 cut(s) | 90, 583, 609 |
| Sfr274I | CTCGAG | 1 cut(s) | 505 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SinI | GGWCC | 3 cut(s) | 193, 406, 581 |
| SlaI | CTCGAG | 1 cut(s) | 505 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SmlI | CTYRAG | 1 cut(s) | 505 |
| SmoI | CTYRAG | 1 cut(s) | 505 |
| Sse9I | AATT | 2 cut(s) | 672, 720 |
| SspMI | CTAG | 4 cut(s) | 188, 513, 578, 611 |
| StyI | CCWWGG | 1 cut(s) | 512 |
| TaaI | ACNGT | 3 cut(s) | 11, 45, 336 |
| TaqI | TCGA | 2 cut(s) | 506, 841 |
| TasI | AATT | 2 cut(s) | 672, 720 |
| TauI | GCSGC | 2 cut(s) | 147, 445 |
| TfiI | GAWTC | 2 cut(s) | 435, 653 |
| Tru1I | TTAA | 1 cut(s) | 641 |
| Tru9I | TTAA | 1 cut(s) | 641 |
| TscAI | CASTG | 1 cut(s) | 495 |
| TseFI | GTSAC | 4 cut(s) | 273, 336, 765, 820 |
| TseI | GCWGC | 3 cut(s) | 141, 412, 622 |
| Tsp45I | GTSAC | 4 cut(s) | 273, 336, 765, 820 |
| TspGWI | ACGGA | 2 cut(s) | 355, 580 |
| TspRI | CASTG | 1 cut(s) | 495 |
| VpaK11BI | GGWCC | 3 cut(s) | 193, 406, 581 |
| XhoI | CTCGAG | 1 cut(s) | 505 |
| XmaJI | CCTAGG | 1 cut(s) | 512 |
| XspI | CTAG | 4 cut(s) | 188, 513, 578, 611 |