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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 90 |
| AciI | CCGC | 7 cut(s) | 9, 15, 48, 74, 88, 145, 149 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 151 |
| AluBI | AGCT | 1 cut(s) | 181 |
| AluI | AGCT | 1 cut(s) | 181 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 2 cut(s) | 108, 154 |
| ApeKI | GCWGC | 3 cut(s) | 12, 138, 141 |
| AspLEI | GCGC | 2 cut(s) | 92, 231 |
| AspS9I | GGNCC | 1 cut(s) | 154 |
| AsuC2I | CCSGG | 2 cut(s) | 107, 117 |
| BbvI | GCAGC | 3 cut(s) | 24, 150, 153 |
| BccI | CCATC | 1 cut(s) | 59 |
| BcnI | CCSGG | 2 cut(s) | 107, 117 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 5 cut(s) | 10, 13, 139, 142, 145 |
| BlsI | GCNGC | 5 cut(s) | 11, 14, 140, 143, 146 |
| Bme1390I | CCNGG | 2 cut(s) | 107, 117 |
| BmgT120I | GGNCC | 1 cut(s) | 154 |
| BmrFI | CCNGG | 2 cut(s) | 107, 117 |
| BpuMI | CCSGG | 2 cut(s) | 107, 117 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 115 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 151 |
| BseDI | CCNNGG | 2 cut(s) | 105, 115 |
| BseGI | GGATG | 1 cut(s) | 51 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 151 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 3 cut(s) | 24, 150, 153 |
| BseYI | CCCAGC | 2 cut(s) | 70, 151 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 2 cut(s) | 110, 156 |
| BsiSI | CCGG | 2 cut(s) | 107, 117 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 151 |
| BsnI | GGCC | 2 cut(s) | 110, 156 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspACI | CCGC | 7 cut(s) | 9, 15, 48, 74, 88, 145, 149 |
| BspANI | GGCC | 2 cut(s) | 110, 156 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BssECI | CCNNGG | 2 cut(s) | 105, 115 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 149, 183 |
| BstF5I | GGATG | 1 cut(s) | 51 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstHHI | GCGC | 2 cut(s) | 92, 231 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 87, 144, 153 |
| BstSCI | CCNGG | 2 cut(s) | 105, 115 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 3 cut(s) | 24, 150, 153 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 2 cut(s) | 110, 156 |
| BtsCI | GGATG | 1 cut(s) | 51 |
| BtsI | GCAGTG | 1 cut(s) | 127 |
| BtsIMutI | CAGTG | 3 cut(s) | 24, 127, 185 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 149, 183 |
| CfoI | GCGC | 2 cut(s) | 92, 231 |
| Cfr13I | GGNCC | 1 cut(s) | 154 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CviJI | RGCY | 6 cut(s) | 110, 144, 156, 181, 242, 248 |
| CviKI_1 | RGCY | 6 cut(s) | 110, 144, 156, 181, 242, 248 |
| CviQI | GTAC | 1 cut(s) | 175 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| FaiI | YATR | 1 cut(s) | 173 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 156 |
| Fnu4HI | GCNGC | 5 cut(s) | 10, 13, 139, 142, 145 |
| FokI | GGATG | 1 cut(s) | 38 |
| Fsp4HI | GCNGC | 5 cut(s) | 10, 13, 139, 142, 145 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GlaI | GCGC | 2 cut(s) | 91, 230 |
| GluI | GCNGC | 5 cut(s) | 10, 13, 139, 142, 145 |
| GsaI | CCCAGC | 2 cut(s) | 74, 155 |
| HaeIII | GGCC | 2 cut(s) | 110, 156 |
| HapII | CCGG | 2 cut(s) | 107, 117 |
| HhaI | GCGC | 2 cut(s) | 92, 231 |
| Hin6I | GCGC | 2 cut(s) | 90, 229 |
| HinP1I | GCGC | 2 cut(s) | 90, 229 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 2 cut(s) | 107, 117 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyCH4IV | ACGT | 1 cut(s) | 217 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 87, 144, 153 |
| HpySE526I | ACGT | 1 cut(s) | 217 |
| HspAI | GCGC | 2 cut(s) | 90, 229 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| LpnPI | CCDG | 6 cut(s) | 20, 84, 120, 130, 137, 167 |
| Lsp1109I | GCAGC | 3 cut(s) | 24, 150, 153 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeII | ACGT | 1 cut(s) | 217 |
| MaeIII | GTNAC | 1 cut(s) | 166 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 4 cut(s) | 73, 118, 214, 217 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 3 cut(s) | 15, 74, 151 |
| MspI | CCGG | 2 cut(s) | 107, 117 |
| MspR9I | CCNGG | 2 cut(s) | 107, 117 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 87, 144, 153 |
| NciI | CCSGG | 2 cut(s) | 107, 117 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| NmuCI | GTSAC | 1 cut(s) | 166 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 5 cut(s) | 11, 14, 140, 143, 146 |
| PspFI | CCCAGC | 2 cut(s) | 70, 151 |
| PspPI | GGNCC | 1 cut(s) | 154 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 5 cut(s) | 10, 13, 139, 142, 145 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 1 cut(s) | 154 |
| ScrFI | CCNGG | 2 cut(s) | 107, 117 |
| SetI | ASST | 3 cut(s) | 129, 183, 220 |
| SsiI | CCGC | 7 cut(s) | 9, 15, 48, 74, 88, 145, 149 |
| SspMI | CTAG | 1 cut(s) | 6 |
| StyD4I | CCNGG | 2 cut(s) | 105, 115 |
| TaiI | ACGT | 1 cut(s) | 220 |
| TauI | GCSGC | 2 cut(s) | 12, 147 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 3 cut(s) | 31, 127, 192 |
| TseFI | GTSAC | 1 cut(s) | 166 |
| TseI | GCWGC | 3 cut(s) | 12, 138, 141 |
| Tsp45I | GTSAC | 1 cut(s) | 166 |
| TspGWI | ACGGA | 1 cut(s) | 206 |
| TspRI | CASTG | 3 cut(s) | 31, 127, 192 |
| XspI | CTAG | 1 cut(s) | 6 |