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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 462 |
| Acc36I | ACCTGC | 1 cut(s) | 347 |
| AccB1I | GGYRCC | 1 cut(s) | 254 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 401 |
| AccI | GTMKAC | 1 cut(s) | 233 |
| AccII | CGCG | 1 cut(s) | 295 |
| AclWI | GGATC | 4 cut(s) | 43, 76, 318, 428 |
| AcoI | YGGCCR | 1 cut(s) | 181 |
| AcuI | CTGAAG | 1 cut(s) | 139 |
| AcyI | GRCGYC | 3 cut(s) | 255, 451, 459 |
| AfaI | GTAC | 1 cut(s) | 51 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 131, 243, 337, 401 |
| AjnI | CCWGG | 5 cut(s) | 8, 31, 222, 257, 308 |
| Alw26I | GTCTC | 1 cut(s) | 438 |
| AlwI | GGATC | 4 cut(s) | 43, 76, 318, 428 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 315 |
| AoxI | GGCC | 5 cut(s) | 29, 181, 261, 367, 494 |
| ApeKI | GCWGC | 3 cut(s) | 13, 16, 138 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 277 |
| AspA2I | CCTAGG | 1 cut(s) | 336 |
| AspLEI | GCGC | 5 cut(s) | 106, 143, 243, 257, 343 |
| AspS9I | GGNCC | 2 cut(s) | 261, 495 |
| AsuC2I | CCSGG | 3 cut(s) | 195, 438, 493 |
| AsuHPI | GGTGA | 2 cut(s) | 35, 333 |
| AvrII | CCTAGG | 1 cut(s) | 336 |
| BanI | GGYRCC | 1 cut(s) | 254 |
| BbvI | GCAGC | 3 cut(s) | 25, 28, 125 |
| BccI | CCATC | 1 cut(s) | 50 |
| BciT130I | CCWGG | 5 cut(s) | 10, 33, 224, 259, 310 |
| BcnI | CCSGG | 3 cut(s) | 195, 438, 493 |
| BcoDI | GTCTC | 1 cut(s) | 438 |
| BfaI | CTAG | 1 cut(s) | 337 |
| BfoI | RGCGCY | 2 cut(s) | 244, 258 |
| BfuAI | ACCTGC | 1 cut(s) | 347 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 28, 353 |
| BisI | GCNGC | 7 cut(s) | 14, 17, 20, 139, 181, 253, 508 |
| BlnI | CCTAGG | 1 cut(s) | 336 |
| BlsI | GCNGC | 7 cut(s) | 15, 18, 21, 140, 182, 254, 509 |
| Bme1390I | CCNGG | 8 cut(s) | 10, 33, 195, 224, 259, 310, 438, 493 |
| BmgT120I | GGNCC | 2 cut(s) | 261, 495 |
| BmiI | GGNNCC | 3 cut(s) | 256, 471, 497 |
| BmrFI | CCNGG | 8 cut(s) | 10, 33, 195, 224, 259, 310, 438, 493 |
| BmrI | ACTGGG | 1 cut(s) | 389 |
| BmsI | GCATC | 1 cut(s) | 400 |
| BmuI | ACTGGG | 1 cut(s) | 389 |
| BoxI | GACNNNNGTC | 1 cut(s) | 450 |
| BpmI | CTGGAG | 1 cut(s) | 441 |
| BpuMI | CCSGG | 3 cut(s) | 195, 438, 493 |
| BsaHI | GRCGYC | 3 cut(s) | 255, 451, 459 |
| BsaJI | CCNNGG | 6 cut(s) | 8, 125, 175, 258, 308, 336 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 131, 243, 337, 401 |
| Bse118I | RCCGGY | 3 cut(s) | 243, 263, 378 |
| Bse1I | ACTGG | 3 cut(s) | 67, 395, 424 |
| BseBI | CCWGG | 5 cut(s) | 10, 33, 224, 259, 310 |
| BseDI | CCNNGG | 6 cut(s) | 8, 125, 175, 258, 308, 336 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 131, 243, 337, 401 |
| BseMII | CTCAG | 1 cut(s) | 307 |
| BseNI | ACTGG | 3 cut(s) | 67, 395, 424 |
| BseRI | GAGGAG | 2 cut(s) | 111, 114 |
| BseXI | GCAGC | 3 cut(s) | 25, 28, 125 |
| BseYI | CCCAGC | 1 cut(s) | 237 |
| Bsh1236I | CGCG | 1 cut(s) | 295 |
| BshFI | GGCC | 5 cut(s) | 31, 183, 263, 369, 496 |
| BshNI | GGYRCC | 1 cut(s) | 254 |
| BsiSI | CCGG | 6 cut(s) | 195, 244, 264, 379, 437, 493 |
| BslFI | GGGAC | 1 cut(s) | 438 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 131, 243, 337, 401 |
| BsmAI | GTCTC | 1 cut(s) | 438 |
| BsmBI | CGTCTC | 1 cut(s) | 438 |
| BsmFI | GGGAC | 1 cut(s) | 438 |
| BsnI | GGCC | 5 cut(s) | 31, 183, 263, 369, 496 |
| Bsp143I | GATC | 4 cut(s) | 35, 68, 323, 433 |
| BspANI | GGCC | 5 cut(s) | 31, 183, 263, 369, 496 |
| BspCNI | CTCAG | 1 cut(s) | 308 |
| BspFNI | CGCG | 1 cut(s) | 295 |
| BspLI | GGNNCC | 3 cut(s) | 256, 471, 497 |
| BspMI | ACCTGC | 1 cut(s) | 347 |
| BspPI | GGATC | 4 cut(s) | 43, 76, 318, 428 |
| BspT107I | GGYRCC | 1 cut(s) | 254 |
| BsrFI | RCCGGY | 3 cut(s) | 243, 263, 378 |
| BsrI | ACTGG | 3 cut(s) | 67, 395, 424 |
| BssAI | RCCGGY | 3 cut(s) | 243, 263, 378 |
| BssECI | CCNNGG | 6 cut(s) | 8, 125, 175, 258, 308, 336 |
| BssMI | GATC | 4 cut(s) | 35, 68, 323, 433 |
| BssNI | GRCGYC | 3 cut(s) | 255, 451, 459 |
| BssT1I | CCWWGG | 3 cut(s) | 125, 175, 336 |
| Bst2UI | CCWGG | 5 cut(s) | 10, 33, 224, 259, 310 |
| BstACI | GRCGYC | 3 cut(s) | 255, 451, 459 |
| BstC8I | GCNNGC | 5 cut(s) | 207, 245, 286, 345, 380 |
| BstDEI | CTNAG | 1 cut(s) | 316 |
| BstFNI | CGCG | 1 cut(s) | 295 |
| BstH2I | RGCGCY | 2 cut(s) | 244, 258 |
| BstHHI | GCGC | 5 cut(s) | 106, 143, 243, 257, 343 |
| BstKTI | GATC | 4 cut(s) | 38, 71, 326, 436 |
| BstMAI | GTCTC | 1 cut(s) | 438 |
| BstMBI | GATC | 4 cut(s) | 35, 68, 323, 433 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 19, 28, 147, 249, 353, 375 |
| BstNI | CCWGG | 5 cut(s) | 10, 33, 224, 259, 310 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 450 |
| BstSCI | CCNGG | 8 cut(s) | 8, 31, 193, 222, 257, 308, 436, 491 |
| BstUI | CGCG | 1 cut(s) | 295 |
| BstV1I | GCAGC | 3 cut(s) | 25, 28, 125 |
| BstX2I | RGATCY | 2 cut(s) | 35, 68 |
| BstYI | RGATCY | 2 cut(s) | 35, 68 |
| BsuRI | GGCC | 5 cut(s) | 31, 183, 263, 369, 496 |
| BtsIMutI | CAGTG | 1 cut(s) | 60 |
| BveI | ACCTGC | 1 cut(s) | 347 |
| Cac8I | GCNNGC | 5 cut(s) | 207, 245, 286, 345, 380 |
| CaiI | CAGNNNCTG | 1 cut(s) | 315 |
| CfoI | GCGC | 5 cut(s) | 106, 143, 243, 257, 343 |
| Cfr10I | RCCGGY | 3 cut(s) | 243, 263, 378 |
| Cfr13I | GGNCC | 2 cut(s) | 261, 495 |
| CseI | GACGC | 1 cut(s) | 440 |
| Csp6I | GTAC | 1 cut(s) | 50 |
| CviAII | CATG | 2 cut(s) | 47, 146 |
| CviQI | GTAC | 1 cut(s) | 50 |
| DdeI | CTNAG | 1 cut(s) | 316 |
| DinI | GGCGCC | 1 cut(s) | 256 |
| DpnI | GATC | 4 cut(s) | 37, 70, 325, 435 |
| DpnII | GATC | 4 cut(s) | 35, 68, 323, 433 |
| EaeI | YGGCCR | 1 cut(s) | 181 |
| EciI | GGCGGA | 6 cut(s) | 81, 285, 316, 365, 456, 494 |
| Eco130I | CCWWGG | 3 cut(s) | 125, 175, 336 |
| Eco57I | CTGAAG | 1 cut(s) | 139 |
| EcoRII | CCWGG | 5 cut(s) | 8, 31, 222, 257, 308 |
| EcoT14I | CCWWGG | 3 cut(s) | 125, 175, 336 |
| EgeI | GGCGCC | 1 cut(s) | 256 |
| EheI | GGCGCC | 1 cut(s) | 256 |
| ErhI | CCWWGG | 3 cut(s) | 125, 175, 336 |
| Esp3I | CGTCTC | 1 cut(s) | 438 |
| FaeI | CATG | 2 cut(s) | 50, 149 |
| FaiI | YATR | 4 cut(s) | 48, 76, 147, 333 |
| FaqI | GGGAC | 1 cut(s) | 438 |
| FatI | CATG | 2 cut(s) | 46, 145 |
| FblI | GTMKAC | 1 cut(s) | 233 |
| Fnu4HI | GCNGC | 7 cut(s) | 14, 17, 20, 139, 181, 253, 508 |
| Fsp4HI | GCNGC | 7 cut(s) | 14, 17, 20, 139, 181, 253, 508 |
| FspBI | CTAG | 1 cut(s) | 337 |
| GlaI | GCGC | 5 cut(s) | 105, 142, 242, 256, 342 |
| GluI | GCNGC | 7 cut(s) | 14, 17, 20, 139, 181, 253, 508 |
| GsaI | CCCAGC | 1 cut(s) | 241 |
| GsuI | CTGGAG | 1 cut(s) | 441 |
| HaeII | RGCGCY | 2 cut(s) | 244, 258 |
| HaeIII | GGCC | 5 cut(s) | 31, 183, 263, 369, 496 |
| HapII | CCGG | 6 cut(s) | 195, 244, 264, 379, 437, 493 |
| HgaI | GACGC | 1 cut(s) | 440 |
| HhaI | GCGC | 5 cut(s) | 106, 143, 243, 257, 343 |
| Hin1I | GRCGYC | 3 cut(s) | 255, 451, 459 |
| Hin1II | CATG | 2 cut(s) | 50, 149 |
| Hin6I | GCGC | 5 cut(s) | 104, 141, 241, 255, 341 |
| HinP1I | GCGC | 5 cut(s) | 104, 141, 241, 255, 341 |
| HincII | GTYRAC | 3 cut(s) | 234, 305, 463 |
| HindII | GTYRAC | 3 cut(s) | 234, 305, 463 |
| HinfI | GANTC | 2 cut(s) | 273, 372 |
| HpaII | CCGG | 6 cut(s) | 195, 244, 264, 379, 437, 493 |
| HphI | GGTGA | 2 cut(s) | 35, 333 |
| Hpy166II | GTNNAC | 3 cut(s) | 234, 305, 463 |
| Hpy188I | TCNGA | 1 cut(s) | 469 |
| Hpy188III | TCNNGA | 2 cut(s) | 455, 487 |
| Hpy8I | GTNNAC | 3 cut(s) | 234, 305, 463 |
| Hpy99I | CGWCG | 1 cut(s) | 461 |
| HpyAV | CCTTC | 2 cut(s) | 107, 288 |
| HpyCH4IV | ACGT | 2 cut(s) | 152, 459 |
| HpyCH4V | TGCA | 1 cut(s) | 413 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 19, 28, 147, 249, 353, 375 |
| HpyF3I | CTNAG | 1 cut(s) | 316 |
| HpySE526I | ACGT | 2 cut(s) | 152, 459 |
| Hsp92I | GRCGYC | 3 cut(s) | 255, 451, 459 |
| Hsp92II | CATG | 2 cut(s) | 50, 149 |
| HspAI | GCGC | 5 cut(s) | 104, 141, 241, 255, 341 |
| KasI | GGCGCC | 1 cut(s) | 254 |
| KroI | GCCGGC | 2 cut(s) | 243, 378 |
| KroNI | GCCGGC | 2 cut(s) | 245, 380 |
| Kzo9I | GATC | 4 cut(s) | 35, 68, 323, 433 |
| LmnI | GCTCC | 2 cut(s) | 101, 422 |
| Lsp1109I | GCAGC | 3 cut(s) | 25, 28, 125 |
| LweI | GCATC | 1 cut(s) | 400 |
| MaeI | CTAG | 1 cut(s) | 337 |
| MaeII | ACGT | 2 cut(s) | 152, 459 |
| MaeIII | GTNAC | 2 cut(s) | 41, 211 |
| MalI | GATC | 4 cut(s) | 37, 70, 325, 435 |
| MboI | GATC | 4 cut(s) | 35, 68, 323, 433 |
| MboII | GAAGA | 1 cut(s) | 63 |
| MflI | RGATCY | 2 cut(s) | 35, 68 |
| Mly113I | GGCGCC | 1 cut(s) | 255 |
| MnlI | CCTC | 4 cut(s) | 89, 92, 152, 311 |
| MreI | CGCCGGCG | 1 cut(s) | 378 |
| MroNI | GCCGGC | 2 cut(s) | 243, 378 |
| MroXI | GAANNNNTTC | 1 cut(s) | 277 |
| MspA1I | CMGCKG | 1 cut(s) | 252 |
| MspI | CCGG | 6 cut(s) | 195, 244, 264, 379, 437, 493 |
| MspR9I | CCNGG | 8 cut(s) | 10, 33, 195, 224, 259, 310, 438, 493 |
| MvaI | CCWGG | 5 cut(s) | 10, 33, 224, 259, 310 |
| MvnI | CGCG | 1 cut(s) | 295 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 19, 28, 147, 249, 353, 375 |
| NaeI | GCCGGC | 2 cut(s) | 245, 380 |
| NarI | GGCGCC | 1 cut(s) | 255 |
| NciI | CCSGG | 3 cut(s) | 195, 438, 493 |
| NdeII | GATC | 4 cut(s) | 35, 68, 323, 433 |
| NgoMIV | GCCGGC | 2 cut(s) | 243, 378 |
| NlaIII | CATG | 2 cut(s) | 50, 149 |
| NlaIV | GGNNCC | 3 cut(s) | 256, 471, 497 |
| NmeAIII | GCCGAG | 1 cut(s) | 332 |
| NmuCI | GTSAC | 2 cut(s) | 41, 211 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 292, 453 |
| PdiI | GCCGGC | 2 cut(s) | 245, 380 |
| PdmI | GAANNNNTTC | 1 cut(s) | 277 |
| PfeI | GAWTC | 2 cut(s) | 273, 372 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 401 |
| PkrI | GCNGC | 7 cut(s) | 15, 18, 21, 140, 182, 254, 509 |
| PluTI | GGCGCC | 1 cut(s) | 258 |
| PshAI | GACNNNNGTC | 1 cut(s) | 450 |
| Psp6I | CCWGG | 5 cut(s) | 8, 31, 222, 257, 308 |
| PspFI | CCCAGC | 1 cut(s) | 237 |
| PspGI | CCWGG | 5 cut(s) | 8, 31, 222, 257, 308 |
| PspN4I | GGNNCC | 3 cut(s) | 256, 471, 497 |
| PspPI | GGNCC | 2 cut(s) | 261, 495 |
| PstNI | CAGNNNCTG | 1 cut(s) | 315 |
| PsuI | RGATCY | 2 cut(s) | 35, 68 |
| RsaI | GTAC | 1 cut(s) | 51 |
| RsaNI | GTAC | 1 cut(s) | 50 |
| SalI | GTCGAC | 1 cut(s) | 232 |
| SatI | GCNGC | 7 cut(s) | 14, 17, 20, 139, 181, 253, 508 |
| Sau3AI | GATC | 4 cut(s) | 35, 68, 323, 433 |
| Sau96I | GGNCC | 2 cut(s) | 261, 495 |
| ScrFI | CCNGG | 8 cut(s) | 10, 33, 195, 224, 259, 310, 438, 493 |
| SetI | ASST | 6 cut(s) | 136, 155, 177, 338, 361, 462 |
| SfaNI | GCATC | 1 cut(s) | 400 |
| SfoI | GGCGCC | 1 cut(s) | 256 |
| SgrAI | CRCCGGYG | 1 cut(s) | 378 |
| SspDI | GGCGCC | 1 cut(s) | 254 |
| SspMI | CTAG | 1 cut(s) | 337 |
| StyD4I | CCNGG | 8 cut(s) | 8, 31, 193, 222, 257, 308, 436, 491 |
| StyI | CCWWGG | 3 cut(s) | 125, 175, 336 |
| TaiI | ACGT | 2 cut(s) | 155, 462 |
| TaqI | TCGA | 2 cut(s) | 157, 233 |
| TauI | GCSGC | 4 cut(s) | 22, 183, 255, 510 |
| TfiI | GAWTC | 2 cut(s) | 273, 372 |
| TscAI | CASTG | 1 cut(s) | 67 |
| TseFI | GTSAC | 2 cut(s) | 41, 211 |
| TseI | GCWGC | 3 cut(s) | 13, 16, 138 |
| Tsp45I | GTSAC | 2 cut(s) | 41, 211 |
| TspDTI | ATGAA | 2 cut(s) | 63, 470 |
| TspRI | CASTG | 1 cut(s) | 67 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 401 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 53 |
| XmaJI | CCTAGG | 1 cut(s) | 336 |
| XmiI | GTMKAC | 1 cut(s) | 233 |
| XmnI | GAANNNNTTC | 1 cut(s) | 277 |
| XspI | CTAG | 1 cut(s) | 337 |
| ZraI | GACGTC | 1 cut(s) | 460 |