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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 148 |
| Acc36I | ACCTGC | 1 cut(s) | 148 |
| AccB1I | GGYRCC | 1 cut(s) | 266 |
| AccII | CGCG | 3 cut(s) | 82, 84, 226 |
| AciI | CCGC | 8 cut(s) | 15, 66, 80, 178, 213, 311, 332, 380 |
| AcoI | YGGCCR | 1 cut(s) | 162 |
| AcsI | RAATTY | 1 cut(s) | 428 |
| AcuI | CTGAAG | 2 cut(s) | 48, 287 |
| AfaI | GTAC | 1 cut(s) | 354 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 69, 157 |
| AgsI | TTSAA | 2 cut(s) | 428, 434 |
| AjnI | CCWGG | 1 cut(s) | 262 |
| AluBI | AGCT | 2 cut(s) | 328, 414 |
| AluI | AGCT | 2 cut(s) | 328, 414 |
| AoxI | GGCC | 3 cut(s) | 141, 147, 162 |
| ApeKI | GCWGC | 1 cut(s) | 313 |
| ApoI | RAATTY | 1 cut(s) | 428 |
| AseI | ATTAAT | 1 cut(s) | 362 |
| AspLEI | GCGC | 3 cut(s) | 84, 86, 226 |
| AspS9I | GGNCC | 3 cut(s) | 148, 307, 341 |
| AsuC2I | CCSGG | 1 cut(s) | 99 |
| AsuHPI | GGTGA | 1 cut(s) | 83 |
| AsuNHI | GCTAGC | 1 cut(s) | 328 |
| AvaII | GGWCC | 2 cut(s) | 307, 341 |
| BalI | TGGCCA | 1 cut(s) | 164 |
| BanI | GGYRCC | 1 cut(s) | 266 |
| BbvI | GCAGC | 1 cut(s) | 300 |
| BccI | CCATC | 2 cut(s) | 59, 102 |
| BceAI | ACGGC | 1 cut(s) | 278 |
| BciT130I | CCWGG | 1 cut(s) | 264 |
| BcnI | CCSGG | 1 cut(s) | 99 |
| BfaI | CTAG | 2 cut(s) | 329, 442 |
| BfuAI | ACCTGC | 1 cut(s) | 148 |
| BisI | GCNGC | 1 cut(s) | 314 |
| BlsI | GCNGC | 1 cut(s) | 315 |
| Bme1390I | CCNGG | 2 cut(s) | 99, 264 |
| Bme18I | GGWCC | 2 cut(s) | 307, 341 |
| BmgT120I | GGNCC | 3 cut(s) | 148, 307, 341 |
| BmiI | GGNNCC | 3 cut(s) | 268, 309, 342 |
| BmrFI | CCNGG | 2 cut(s) | 99, 264 |
| BmsI | GCATC | 2 cut(s) | 82, 307 |
| BmtI | GCTAGC | 1 cut(s) | 332 |
| BpuMI | CCSGG | 1 cut(s) | 99 |
| BsaJI | CCNNGG | 1 cut(s) | 144 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 368, 398 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 69, 157 |
| BseBI | CCWGG | 1 cut(s) | 264 |
| BseDI | CCNNGG | 1 cut(s) | 144 |
| BseGI | GGATG | 2 cut(s) | 51, 94 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 69, 157 |
| BsePI | GCGCGC | 1 cut(s) | 82 |
| BseXI | GCAGC | 1 cut(s) | 300 |
| BseYI | CCCAGC | 3 cut(s) | 62, 108, 220 |
| BsgI | GTGCAG | 1 cut(s) | 299 |
| Bsh1236I | CGCG | 3 cut(s) | 82, 84, 226 |
| BshFI | GGCC | 3 cut(s) | 143, 149, 164 |
| BshNI | GGYRCC | 1 cut(s) | 266 |
| BsiSI | CCGG | 1 cut(s) | 99 |
| BslFI | GGGAC | 3 cut(s) | 160, 293, 354 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 69, 157 |
| BsmFI | GGGAC | 3 cut(s) | 160, 293, 354 |
| BsnI | GGCC | 3 cut(s) | 143, 149, 164 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 8 cut(s) | 15, 66, 80, 178, 213, 311, 332, 380 |
| BspANI | GGCC | 3 cut(s) | 143, 149, 164 |
| BspFNI | CGCG | 3 cut(s) | 82, 84, 226 |
| BspLI | GGNNCC | 3 cut(s) | 268, 309, 342 |
| BspMI | ACCTGC | 1 cut(s) | 148 |
| BspOI | GCTAGC | 1 cut(s) | 332 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 266 |
| BssECI | CCNNGG | 1 cut(s) | 144 |
| BssHII | GCGCGC | 1 cut(s) | 82 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 1 cut(s) | 264 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 84, 233, 330 |
| BstDEI | CTNAG | 1 cut(s) | 407 |
| BstF5I | GGATG | 2 cut(s) | 51, 94 |
| BstFNI | CGCG | 3 cut(s) | 82, 84, 226 |
| BstHHI | GCGC | 3 cut(s) | 84, 86, 226 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 79, 221, 232 |
| BstNI | CCWGG | 1 cut(s) | 264 |
| BstSCI | CCNGG | 2 cut(s) | 97, 262 |
| BstUI | CGCG | 3 cut(s) | 82, 84, 226 |
| BstV1I | GCAGC | 1 cut(s) | 300 |
| BsuRI | GGCC | 3 cut(s) | 143, 149, 164 |
| BtsCI | GGATG | 2 cut(s) | 51, 94 |
| BveI | ACCTGC | 1 cut(s) | 148 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 84, 233, 330 |
| CfoI | GCGC | 3 cut(s) | 84, 86, 226 |
| Cfr13I | GGNCC | 3 cut(s) | 148, 307, 341 |
| CsiI | ACCWGGT | 1 cut(s) | 262 |
| Csp6I | GTAC | 1 cut(s) | 353 |
| CviJI | RGCY | 8 cut(s) | 143, 149, 164, 209, 258, 293, 328, 414 |
| CviKI_1 | RGCY | 8 cut(s) | 143, 149, 164, 209, 258, 293, 328, 414 |
| CviQI | GTAC | 1 cut(s) | 353 |
| DdeI | CTNAG | 1 cut(s) | 407 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 162 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 395 |
| Eco47I | GGWCC | 2 cut(s) | 307, 341 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 287 |
| EcoO109I | RGGNCCY | 1 cut(s) | 307 |
| EcoRII | CCWGG | 1 cut(s) | 262 |
| FaiI | YATR | 1 cut(s) | 173 |
| FaqI | GGGAC | 3 cut(s) | 160, 293, 354 |
| FauI | CCCGC | 4 cut(s) | 8, 185, 318, 325 |
| Fnu4HI | GCNGC | 1 cut(s) | 314 |
| FokI | GGATG | 2 cut(s) | 38, 81 |
| Fsp4HI | GCNGC | 1 cut(s) | 314 |
| FspBI | CTAG | 2 cut(s) | 329, 442 |
| GlaI | GCGC | 3 cut(s) | 83, 85, 225 |
| GluI | GCNGC | 1 cut(s) | 314 |
| GsaI | CCCAGC | 3 cut(s) | 66, 112, 224 |
| HaeIII | GGCC | 3 cut(s) | 143, 149, 164 |
| HapII | CCGG | 1 cut(s) | 99 |
| HhaI | GCGC | 3 cut(s) | 84, 86, 226 |
| Hin6I | GCGC | 3 cut(s) | 82, 84, 224 |
| HinP1I | GCGC | 3 cut(s) | 82, 84, 224 |
| HincII | GTYRAC | 1 cut(s) | 104 |
| HindII | GTYRAC | 1 cut(s) | 104 |
| HindIII | AAGCTT | 1 cut(s) | 412 |
| HinfI | GANTC | 1 cut(s) | 383 |
| HpaII | CCGG | 1 cut(s) | 99 |
| HphI | GGTGA | 1 cut(s) | 83 |
| Hpy166II | GTNNAC | 2 cut(s) | 104, 122 |
| Hpy188III | TCNNGA | 1 cut(s) | 240 |
| Hpy8I | GTNNAC | 2 cut(s) | 104, 122 |
| HpyCH4IV | ACGT | 1 cut(s) | 118 |
| HpyCH4V | TGCA | 2 cut(s) | 298, 316 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 79, 221, 232 |
| HpyF3I | CTNAG | 1 cut(s) | 407 |
| HpySE526I | ACGT | 1 cut(s) | 118 |
| HspAI | GCGC | 3 cut(s) | 82, 84, 224 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| Lsp1109I | GCAGC | 1 cut(s) | 300 |
| LweI | GCATC | 2 cut(s) | 82, 307 |
| MabI | ACCWGGT | 1 cut(s) | 262 |
| MaeI | CTAG | 2 cut(s) | 329, 442 |
| MaeII | ACGT | 1 cut(s) | 118 |
| MaeIII | GTNAC | 1 cut(s) | 237 |
| MalI | GATC | 1 cut(s) | 42 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 2 cut(s) | 41, 293 |
| MfeI | CAATTG | 1 cut(s) | 434 |
| MlsI | TGGCCA | 1 cut(s) | 164 |
| MluCI | AATT | 3 cut(s) | 359, 428, 434 |
| MluNI | TGGCCA | 1 cut(s) | 164 |
| MlyI | GAGTC | 1 cut(s) | 392 |
| MmeI | TCCRAC | 1 cut(s) | 348 |
| MnlI | CCTC | 3 cut(s) | 139, 370, 401 |
| Mox20I | TGGCCA | 1 cut(s) | 164 |
| MscI | TGGCCA | 1 cut(s) | 164 |
| MseI | TTAA | 2 cut(s) | 191, 362 |
| MslI | CAYNNNNRTG | 1 cut(s) | 170 |
| Msp20I | TGGCCA | 1 cut(s) | 164 |
| MspA1I | CMGCKG | 3 cut(s) | 15, 66, 313 |
| MspI | CCGG | 1 cut(s) | 99 |
| MspR9I | CCNGG | 2 cut(s) | 99, 264 |
| MunI | CAATTG | 1 cut(s) | 434 |
| MvaI | CCWGG | 1 cut(s) | 264 |
| MvnI | CGCG | 3 cut(s) | 82, 84, 226 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 79, 221, 232 |
| NciI | CCSGG | 1 cut(s) | 99 |
| NdeII | GATC | 1 cut(s) | 40 |
| NheI | GCTAGC | 1 cut(s) | 328 |
| NlaIV | GGNNCC | 3 cut(s) | 268, 309, 342 |
| NmeAIII | GCCGAG | 1 cut(s) | 169 |
| NmuCI | GTSAC | 1 cut(s) | 237 |
| PaqCI | CACCTGC | 1 cut(s) | 148 |
| PauI | GCGCGC | 1 cut(s) | 82 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 1 cut(s) | 315 |
| PleI | GAGTC | 1 cut(s) | 391 |
| PpsI | GAGTC | 1 cut(s) | 391 |
| PpuMI | RGGWCCY | 1 cut(s) | 307 |
| PshBI | ATTAAT | 1 cut(s) | 362 |
| Psp5II | RGGWCCY | 1 cut(s) | 307 |
| Psp6I | CCWGG | 1 cut(s) | 262 |
| PspFI | CCCAGC | 3 cut(s) | 62, 108, 220 |
| PspGI | CCWGG | 1 cut(s) | 262 |
| PspN4I | GGNNCC | 3 cut(s) | 268, 309, 342 |
| PspPI | GGNCC | 3 cut(s) | 148, 307, 341 |
| PspPPI | RGGWCCY | 1 cut(s) | 307 |
| PteI | GCGCGC | 1 cut(s) | 82 |
| RsaI | GTAC | 1 cut(s) | 354 |
| RsaNI | GTAC | 1 cut(s) | 353 |
| RseI | CAYNNNNRTG | 1 cut(s) | 170 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 2 cut(s) | 191, 362 |
| SatI | GCNGC | 1 cut(s) | 314 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 3 cut(s) | 148, 307, 341 |
| SchI | GAGTC | 1 cut(s) | 392 |
| ScrFI | CCNGG | 2 cut(s) | 99, 264 |
| SetI | ASST | 6 cut(s) | 121, 162, 265, 309, 330, 416 |
| SexAI | ACCWGGT | 1 cut(s) | 262 |
| SfaNI | GCATC | 2 cut(s) | 82, 307 |
| SinI | GGWCC | 2 cut(s) | 307, 341 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 170 |
| Sse9I | AATT | 3 cut(s) | 359, 428, 434 |
| SsiI | CCGC | 8 cut(s) | 15, 66, 80, 178, 213, 311, 332, 380 |
| SspMI | CTAG | 2 cut(s) | 329, 442 |
| StyD4I | CCNGG | 2 cut(s) | 97, 262 |
| TaiI | ACGT | 1 cut(s) | 121 |
| TasI | AATT | 3 cut(s) | 359, 428, 434 |
| Tru1I | TTAA | 2 cut(s) | 191, 362 |
| Tru9I | TTAA | 2 cut(s) | 191, 362 |
| TseFI | GTSAC | 1 cut(s) | 237 |
| TseI | GCWGC | 1 cut(s) | 313 |
| Tsp45I | GTSAC | 1 cut(s) | 237 |
| TspDTI | ATGAA | 1 cut(s) | 391 |
| VpaK11BI | GGWCC | 2 cut(s) | 307, 341 |
| VspI | ATTAAT | 1 cut(s) | 362 |
| XapI | RAATTY | 1 cut(s) | 428 |
| XspI | CTAG | 2 cut(s) | 329, 442 |