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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 483 |
| Acc36I | ACCTGC | 2 cut(s) | 483, 576 |
| AccB1I | GGYRCC | 1 cut(s) | 399 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 630 |
| AccBSI | CCGCTC | 1 cut(s) | 524 |
| AccI | GTMKAC | 1 cut(s) | 378 |
| AccII | CGCG | 5 cut(s) | 94, 110, 112, 286, 432 |
| AclWI | GGATC | 4 cut(s) | 221, 547, 657, 802 |
| AcoI | YGGCCR | 3 cut(s) | 128, 281, 326 |
| AcyI | GRCGYC | 2 cut(s) | 400, 680 |
| AfaI | GTAC | 3 cut(s) | 196, 428, 563 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 97, 388, 539, 630 |
| AflIII | ACRYGT | 1 cut(s) | 295 |
| AgeI | ACCGGT | 1 cut(s) | 408 |
| AgsI | TTSAA | 1 cut(s) | 834 |
| AjnI | CCWGG | 5 cut(s) | 153, 367, 402, 441, 532 |
| AluBI | AGCT | 1 cut(s) | 63 |
| AluI | AGCT | 1 cut(s) | 63 |
| Alw21I | GWGCWC | 1 cut(s) | 65 |
| Alw26I | GTCTC | 2 cut(s) | 267, 667 |
| AlwI | GGATC | 4 cut(s) | 221, 547, 657, 802 |
| AoxI | GGCC | 9 cut(s) | 128, 174, 266, 281, 326, 596, 723, 749, 757 |
| ApeKI | GCWGC | 3 cut(s) | 158, 161, 362 |
| AsiGI | ACCGGT | 1 cut(s) | 408 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 506 |
| AspLEI | GCGC | 8 cut(s) | 112, 114, 251, 288, 374, 388, 402, 572 |
| AspS9I | GGNCC | 4 cut(s) | 267, 406, 460, 724 |
| AsuC2I | CCSGG | 5 cut(s) | 127, 137, 340, 667, 722 |
| AsuHPI | GGTGA | 4 cut(s) | 9, 154, 180, 834 |
| AvaII | GGWCC | 2 cut(s) | 406, 460 |
| BanI | GGYRCC | 1 cut(s) | 399 |
| BanII | GRGCYC | 1 cut(s) | 65 |
| Bbv12I | GWGCWC | 1 cut(s) | 65 |
| BbvCI | CCTCAGC | 2 cut(s) | 47, 263 |
| BbvI | GCAGC | 3 cut(s) | 170, 173, 374 |
| BccI | CCATC | 1 cut(s) | 273 |
| BceAI | ACGGC | 1 cut(s) | 736 |
| BciT130I | CCWGG | 5 cut(s) | 155, 369, 404, 443, 534 |
| BcnI | CCSGG | 5 cut(s) | 127, 137, 340, 667, 722 |
| BcoDI | GTCTC | 2 cut(s) | 267, 667 |
| BfaI | CTAG | 1 cut(s) | 649 |
| BfmI | CTRYAG | 1 cut(s) | 745 |
| BfoI | RGCGCY | 2 cut(s) | 389, 403 |
| BfuAI | ACCTGC | 2 cut(s) | 483, 576 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 173, 582 |
| BglII | AGATCT | 1 cut(s) | 180 |
| Bme18I | GGWCC | 2 cut(s) | 406, 460 |
| BmgT120I | GGNCC | 4 cut(s) | 267, 406, 460, 724 |
| BmiI | GGNNCC | 5 cut(s) | 401, 436, 461, 700, 726 |
| BmrI | ACTGGG | 1 cut(s) | 618 |
| BmsI | GCATC | 1 cut(s) | 629 |
| BmuI | ACTGGG | 1 cut(s) | 618 |
| BoxI | GACNNNNGTC | 1 cut(s) | 679 |
| Bpu10I | CCTNAGC | 3 cut(s) | 47, 263, 566 |
| BpuMI | CCSGG | 5 cut(s) | 127, 137, 340, 667, 722 |
| BsaAI | YACGTR | 1 cut(s) | 426 |
| BsaHI | GRCGYC | 2 cut(s) | 400, 680 |
| BsaJI | CCNNGG | 7 cut(s) | 20, 125, 135, 320, 430, 442, 533 |
| BsaWI | WCCGGW | 1 cut(s) | 408 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 97, 388, 539, 630 |
| Bse118I | RCCGGY | 3 cut(s) | 388, 408, 607 |
| Bse1I | ACTGG | 2 cut(s) | 212, 624 |
| BseBI | CCWGG | 5 cut(s) | 155, 369, 404, 443, 534 |
| BseDI | CCNNGG | 7 cut(s) | 20, 125, 135, 320, 430, 442, 533 |
| BseGI | GGATG | 3 cut(s) | 555, 644, 806 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 97, 388, 539, 630 |
| BseMII | CTCAG | 4 cut(s) | 38, 254, 536, 557 |
| BseNI | ACTGG | 2 cut(s) | 212, 624 |
| BsePI | GCGCGC | 1 cut(s) | 110 |
| BseRI | GAGGAG | 3 cut(s) | 64, 256, 259 |
| BseXI | GCAGC | 3 cut(s) | 170, 173, 374 |
| BseYI | CCCAGC | 1 cut(s) | 382 |
| Bsh1236I | CGCG | 5 cut(s) | 94, 110, 112, 286, 432 |
| Bsh1285I | CGRYCG | 1 cut(s) | 98 |
| BshFI | GGCC | 9 cut(s) | 130, 176, 268, 283, 328, 598, 725, 751, 759 |
| BshNI | GGYRCC | 1 cut(s) | 399 |
| BshTI | ACCGGT | 1 cut(s) | 408 |
| BsiEI | CGRYCG | 1 cut(s) | 98 |
| BsiHKAI | GWGCWC | 1 cut(s) | 65 |
| BsiSI | CCGG | 8 cut(s) | 127, 137, 340, 389, 409, 608, 666, 722 |
| BslFI | GGGAC | 2 cut(s) | 473, 667 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 97, 388, 539, 630 |
| BsmAI | GTCTC | 2 cut(s) | 267, 667 |
| BsmBI | CGTCTC | 1 cut(s) | 667 |
| BsmFI | GGGAC | 2 cut(s) | 473, 667 |
| BsnI | GGCC | 9 cut(s) | 130, 176, 268, 283, 328, 598, 725, 751, 759 |
| Bsp1286I | GDGCHC | 1 cut(s) | 65 |
| Bsp143I | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| Bsp19I | CCATGG | 1 cut(s) | 20 |
| BspANI | GGCC | 9 cut(s) | 130, 176, 268, 283, 328, 598, 725, 751, 759 |
| BspCNI | CTCAG | 4 cut(s) | 39, 255, 537, 558 |
| BspFNI | CGCG | 5 cut(s) | 94, 110, 112, 286, 432 |
| BspHI | TCATGA | 1 cut(s) | 290 |
| BspLI | GGNNCC | 5 cut(s) | 401, 436, 461, 700, 726 |
| BspMAI | CTGCAG | 1 cut(s) | 749 |
| BspMI | ACCTGC | 2 cut(s) | 483, 576 |
| BspPI | GGATC | 4 cut(s) | 221, 547, 657, 802 |
| BspT107I | GGYRCC | 1 cut(s) | 399 |
| BsrBI | CCGCTC | 1 cut(s) | 524 |
| BsrFI | RCCGGY | 3 cut(s) | 388, 408, 607 |
| BsrI | ACTGG | 2 cut(s) | 212, 624 |
| BssAI | RCCGGY | 3 cut(s) | 388, 408, 607 |
| BssECI | CCNNGG | 7 cut(s) | 20, 125, 135, 320, 430, 442, 533 |
| BssHII | GCGCGC | 1 cut(s) | 110 |
| BssMI | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| BssNI | GRCGYC | 2 cut(s) | 400, 680 |
| BssT1I | CCWWGG | 2 cut(s) | 20, 320 |
| Bst2UI | CCWGG | 5 cut(s) | 155, 369, 404, 443, 534 |
| Bst4CI | ACNGT | 1 cut(s) | 31 |
| BstACI | GRCGYC | 2 cut(s) | 400, 680 |
| BstBAI | YACGTR | 1 cut(s) | 426 |
| BstC8I | GCNNGC | 7 cut(s) | 56, 112, 352, 390, 574, 609, 749 |
| BstDEI | CTNAG | 4 cut(s) | 47, 263, 545, 566 |
| BstDSI | CCRYGG | 2 cut(s) | 20, 430 |
| BstEII | GGTNACC | 1 cut(s) | 840 |
| BstF5I | GGATG | 3 cut(s) | 555, 644, 806 |
| BstFNI | CGCG | 5 cut(s) | 94, 110, 112, 286, 432 |
| BstH2I | RGCGCY | 2 cut(s) | 389, 403 |
| BstHHI | GCGC | 8 cut(s) | 112, 114, 251, 288, 374, 388, 402, 572 |
| BstKTI | GATC | 6 cut(s) | 98, 183, 216, 555, 665, 810 |
| BstMAI | GTCTC | 2 cut(s) | 267, 667 |
| BstMBI | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| BstMCI | CGRYCG | 1 cut(s) | 98 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 60, 107, 164, 173, 371, 394, 429, 582, 604 |
| BstNI | CCWGG | 5 cut(s) | 155, 369, 404, 443, 534 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 679 |
| BstPI | GGTNACC | 1 cut(s) | 840 |
| BstSFI | CTRYAG | 1 cut(s) | 745 |
| BstUI | CGCG | 5 cut(s) | 94, 110, 112, 286, 432 |
| BstV1I | GCAGC | 3 cut(s) | 170, 173, 374 |
| BstX2I | RGATCY | 2 cut(s) | 180, 213 |
| BstYI | RGATCY | 2 cut(s) | 180, 213 |
| BsuRI | GGCC | 9 cut(s) | 130, 176, 268, 283, 328, 598, 725, 751, 759 |
| BtgI | CCRYGG | 2 cut(s) | 20, 430 |
| BtsCI | GGATG | 3 cut(s) | 555, 644, 806 |
| BtsIMutI | CAGTG | 1 cut(s) | 205 |
| BveI | ACCTGC | 2 cut(s) | 483, 576 |
| Cac8I | GCNNGC | 7 cut(s) | 56, 112, 352, 390, 574, 609, 749 |
| CciI | TCATGA | 1 cut(s) | 290 |
| CfoI | GCGC | 8 cut(s) | 112, 114, 251, 288, 374, 388, 402, 572 |
| Cfr10I | RCCGGY | 3 cut(s) | 388, 408, 607 |
| Cfr13I | GGNCC | 4 cut(s) | 267, 406, 460, 724 |
| Cfr42I | CCGCGG | 1 cut(s) | 433 |
| CseI | GACGC | 1 cut(s) | 669 |
| Csp6I | GTAC | 3 cut(s) | 195, 427, 562 |
| CspAI | ACCGGT | 1 cut(s) | 408 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 428, 463 |
| CviAII | CATG | 4 cut(s) | 21, 192, 291, 822 |
| CviQI | GTAC | 3 cut(s) | 195, 427, 562 |
| DdeI | CTNAG | 4 cut(s) | 47, 263, 545, 566 |
| DinI | GGCGCC | 1 cut(s) | 401 |
| DpnI | GATC | 6 cut(s) | 97, 182, 215, 554, 664, 809 |
| DpnII | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| EaeI | YGGCCR | 3 cut(s) | 128, 281, 326 |
| EciI | GGCGGA | 6 cut(s) | 226, 514, 545, 594, 685, 723 |
| Ecl136II | GAGCTC | 1 cut(s) | 63 |
| Eco130I | CCWWGG | 2 cut(s) | 20, 320 |
| Eco24I | GRGCYC | 1 cut(s) | 65 |
| Eco47I | GGWCC | 2 cut(s) | 406, 460 |
| Eco53kI | GAGCTC | 1 cut(s) | 63 |
| Eco91I | GGTNACC | 1 cut(s) | 840 |
| EcoICRI | GAGCTC | 1 cut(s) | 63 |
| EcoO65I | GGTNACC | 1 cut(s) | 840 |
| EcoRII | CCWGG | 5 cut(s) | 153, 367, 402, 441, 532 |
| EcoT14I | CCWWGG | 2 cut(s) | 20, 320 |
| EcoT38I | GRGCYC | 1 cut(s) | 65 |
| EgeI | GGCGCC | 1 cut(s) | 401 |
| EheI | GGCGCC | 1 cut(s) | 401 |
| ErhI | CCWWGG | 2 cut(s) | 20, 320 |
| Esp3I | CGTCTC | 1 cut(s) | 667 |
| FaeI | CATG | 4 cut(s) | 24, 195, 294, 825 |
| FaqI | GGGAC | 2 cut(s) | 473, 667 |
| FatI | CATG | 4 cut(s) | 20, 191, 290, 821 |
| FauI | CCCGC | 4 cut(s) | 28, 75, 99, 790 |
| FauNDI | CATATG | 1 cut(s) | 765 |
| FblI | GTMKAC | 1 cut(s) | 378 |
| FokI | GGATG | 3 cut(s) | 562, 651, 813 |
| FriOI | GRGCYC | 1 cut(s) | 65 |
| FspBI | CTAG | 1 cut(s) | 649 |
| GlaI | GCGC | 8 cut(s) | 111, 113, 250, 287, 373, 387, 401, 571 |
| GsaI | CCCAGC | 1 cut(s) | 386 |
| HaeII | RGCGCY | 2 cut(s) | 389, 403 |
| HaeIII | GGCC | 9 cut(s) | 130, 176, 268, 283, 328, 598, 725, 751, 759 |
| HapII | CCGG | 8 cut(s) | 127, 137, 340, 389, 409, 608, 666, 722 |
| HgaI | GACGC | 1 cut(s) | 669 |
| HhaI | GCGC | 8 cut(s) | 112, 114, 251, 288, 374, 388, 402, 572 |
| Hin1I | GRCGYC | 2 cut(s) | 400, 680 |
| Hin1II | CATG | 4 cut(s) | 24, 195, 294, 825 |
| Hin6I | GCGC | 8 cut(s) | 110, 112, 249, 286, 372, 386, 400, 570 |
| HinP1I | GCGC | 8 cut(s) | 110, 112, 249, 286, 372, 386, 400, 570 |
| HincII | GTYRAC | 1 cut(s) | 379 |
| HindII | GTYRAC | 1 cut(s) | 379 |
| HinfI | GANTC | 2 cut(s) | 502, 601 |
| HpaII | CCGG | 8 cut(s) | 127, 137, 340, 389, 409, 608, 666, 722 |
| HphI | GGTGA | 4 cut(s) | 9, 154, 180, 834 |
| Hpy166II | GTNNAC | 2 cut(s) | 379, 819 |
| Hpy188I | TCNGA | 2 cut(s) | 87, 698 |
| Hpy188III | TCNNGA | 5 cut(s) | 291, 452, 684, 716, 834 |
| Hpy8I | GTNNAC | 2 cut(s) | 379, 819 |
| HpyAV | CCTTC | 3 cut(s) | 252, 831, 840 |
| HpyCH4III | ACNGT | 1 cut(s) | 31 |
| HpyCH4IV | ACGT | 2 cut(s) | 297, 425 |
| HpyCH4V | TGCA | 3 cut(s) | 423, 642, 747 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 60, 107, 164, 173, 371, 394, 429, 582, 604 |
| HpyF3I | CTNAG | 4 cut(s) | 47, 263, 545, 566 |
| HpySE526I | ACGT | 2 cut(s) | 297, 425 |
| Hsp92I | GRCGYC | 2 cut(s) | 400, 680 |
| Hsp92II | CATG | 4 cut(s) | 24, 195, 294, 825 |
| HspAI | GCGC | 8 cut(s) | 110, 112, 249, 286, 372, 386, 400, 570 |
| KasI | GGCGCC | 1 cut(s) | 399 |
| KroI | GCCGGC | 2 cut(s) | 388, 607 |
| KroNI | GCCGGC | 2 cut(s) | 390, 609 |
| KspI | CCGCGG | 1 cut(s) | 433 |
| Kzo9I | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| LmnI | GCTCC | 4 cut(s) | 51, 68, 234, 246 |
| Lsp1109I | GCAGC | 3 cut(s) | 170, 173, 374 |
| LweI | GCATC | 1 cut(s) | 629 |
| MaeI | CTAG | 1 cut(s) | 649 |
| MaeII | ACGT | 2 cut(s) | 297, 425 |
| MaeIII | GTNAC | 4 cut(s) | 186, 356, 785, 840 |
| MalI | GATC | 6 cut(s) | 97, 182, 215, 554, 664, 809 |
| MbiI | CCGCTC | 1 cut(s) | 524 |
| MboI | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| MboII | GAAGA | 1 cut(s) | 208 |
| MflI | RGATCY | 2 cut(s) | 180, 213 |
| MhlI | GDGCHC | 1 cut(s) | 65 |
| Mly113I | GGCGCC | 1 cut(s) | 400 |
| MmeI | TCCRAC | 1 cut(s) | 110 |
| MnlI | CCTC | 8 cut(s) | 42, 93, 234, 237, 258, 297, 540, 650 |
| MreI | CGCCGGCG | 1 cut(s) | 607 |
| MroNI | GCCGGC | 2 cut(s) | 388, 607 |
| MroXI | GAANNNNTTC | 1 cut(s) | 506 |
| MseI | TTAA | 1 cut(s) | 104 |
| MslI | CAYNNNNRTG | 1 cut(s) | 418 |
| MspA1I | CMGCKG | 2 cut(s) | 397, 432 |
| MspI | CCGG | 8 cut(s) | 127, 137, 340, 389, 409, 608, 666, 722 |
| MvaI | CCWGG | 5 cut(s) | 155, 369, 404, 443, 534 |
| MvnI | CGCG | 5 cut(s) | 94, 110, 112, 286, 432 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 60, 107, 164, 173, 371, 394, 429, 582, 604 |
| NaeI | GCCGGC | 2 cut(s) | 390, 609 |
| NarI | GGCGCC | 1 cut(s) | 400 |
| NciI | CCSGG | 5 cut(s) | 127, 137, 340, 667, 722 |
| NcoI | CCATGG | 1 cut(s) | 20 |
| NdeI | CATATG | 1 cut(s) | 765 |
| NdeII | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| NgoMIV | GCCGGC | 2 cut(s) | 388, 607 |
| NlaIII | CATG | 4 cut(s) | 24, 195, 294, 825 |
| NlaIV | GGNNCC | 5 cut(s) | 401, 436, 461, 700, 726 |
| NmeAIII | GCCGAG | 1 cut(s) | 561 |
| NmuCI | GTSAC | 4 cut(s) | 186, 356, 785, 840 |
| PagI | TCATGA | 1 cut(s) | 290 |
| PaqCI | CACCTGC | 1 cut(s) | 483 |
| PauI | GCGCGC | 1 cut(s) | 110 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 682 |
| PdiI | GCCGGC | 2 cut(s) | 390, 609 |
| PdmI | GAANNNNTTC | 1 cut(s) | 506 |
| PfeI | GAWTC | 2 cut(s) | 502, 601 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 630 |
| PinAI | ACCGGT | 1 cut(s) | 408 |
| Ple19I | CGATCG | 1 cut(s) | 98 |
| PluTI | GGCGCC | 1 cut(s) | 403 |
| Ppu21I | YACGTR | 1 cut(s) | 426 |
| PshAI | GACNNNNGTC | 1 cut(s) | 679 |
| Psp124BI | GAGCTC | 1 cut(s) | 65 |
| Psp6I | CCWGG | 5 cut(s) | 153, 367, 402, 441, 532 |
| PspEI | GGTNACC | 1 cut(s) | 840 |
| PspFI | CCCAGC | 1 cut(s) | 382 |
| PspGI | CCWGG | 5 cut(s) | 153, 367, 402, 441, 532 |
| PspN4I | GGNNCC | 5 cut(s) | 401, 436, 461, 700, 726 |
| PspPI | GGNCC | 4 cut(s) | 267, 406, 460, 724 |
| PstI | CTGCAG | 1 cut(s) | 749 |
| PsuI | RGATCY | 2 cut(s) | 180, 213 |
| PteI | GCGCGC | 1 cut(s) | 110 |
| PvuI | CGATCG | 1 cut(s) | 98 |
| RsaI | GTAC | 3 cut(s) | 196, 428, 563 |
| RsaNI | GTAC | 3 cut(s) | 195, 427, 562 |
| RseI | CAYNNNNRTG | 1 cut(s) | 418 |
| SacI | GAGCTC | 1 cut(s) | 65 |
| SacII | CCGCGG | 1 cut(s) | 433 |
| SalI | GTCGAC | 1 cut(s) | 377 |
| SaqAI | TTAA | 1 cut(s) | 104 |
| Sau3AI | GATC | 6 cut(s) | 95, 180, 213, 552, 662, 807 |
| Sau96I | GGNCC | 4 cut(s) | 267, 406, 460, 724 |
| SbfI | CCTGCAGG | 1 cut(s) | 749 |
| SdaI | CCTGCAGG | 1 cut(s) | 749 |
| SduI | GDGCHC | 1 cut(s) | 65 |
| SfaNI | GCATC | 1 cut(s) | 629 |
| SfcI | CTRYAG | 1 cut(s) | 745 |
| SfoI | GGCGCC | 1 cut(s) | 401 |
| Sfr303I | CCGCGG | 1 cut(s) | 433 |
| SgrAI | CRCCGGYG | 1 cut(s) | 607 |
| SgrBI | CCGCGG | 1 cut(s) | 433 |
| SinI | GGWCC | 2 cut(s) | 406, 460 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 418 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 749 |
| SspDI | GGCGCC | 1 cut(s) | 399 |
| SspMI | CTAG | 1 cut(s) | 649 |
| SstI | GAGCTC | 1 cut(s) | 65 |
| StyI | CCWWGG | 2 cut(s) | 20, 320 |
| TaaI | ACNGT | 1 cut(s) | 31 |
| TaiI | ACGT | 2 cut(s) | 300, 428 |
| TaqI | TCGA | 2 cut(s) | 378, 526 |
| TaqII | GACCGA | 1 cut(s) | 820 |
| TauI | GCSGC | 7 cut(s) | 167, 286, 328, 400, 468, 622, 739 |
| TfiI | GAWTC | 2 cut(s) | 502, 601 |
| Tru1I | TTAA | 1 cut(s) | 104 |
| Tru9I | TTAA | 1 cut(s) | 104 |
| TscAI | CASTG | 1 cut(s) | 212 |
| TseFI | GTSAC | 4 cut(s) | 186, 356, 785, 840 |
| TseI | GCWGC | 3 cut(s) | 158, 161, 362 |
| Tsp45I | GTSAC | 4 cut(s) | 186, 356, 785, 840 |
| TspDTI | ATGAA | 2 cut(s) | 208, 699 |
| TspRI | CASTG | 1 cut(s) | 212 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 630 |
| VpaK11BI | GGWCC | 2 cut(s) | 406, 460 |
| XmiI | GTMKAC | 1 cut(s) | 378 |
| XmnI | GAANNNNTTC | 1 cut(s) | 506 |
| XspI | CTAG | 1 cut(s) | 649 |