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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 152 |
| Acc65I | GGTACC | 1 cut(s) | 164 |
| AccB1I | GGYRCC | 1 cut(s) | 164 |
| AccBSI | CCGCTC | 1 cut(s) | 262 |
| AciI | CCGC | 4 cut(s) | 168, 237, 260, 265 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AfaI | GTAC | 1 cut(s) | 166 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 31 |
| AluBI | AGCT | 4 cut(s) | 10, 77, 118, 185 |
| AluI | AGCT | 4 cut(s) | 10, 77, 118, 185 |
| Alw21I | GWGCWC | 1 cut(s) | 48 |
| AoxI | GGCC | 2 cut(s) | 69, 90 |
| ApeKI | GCWGC | 1 cut(s) | 10 |
| Asp718I | GGTACC | 1 cut(s) | 164 |
| AspLEI | GCGC | 2 cut(s) | 130, 154 |
| AspS9I | GGNCC | 1 cut(s) | 198 |
| AvaII | GGWCC | 1 cut(s) | 198 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BanI | GGYRCC | 1 cut(s) | 164 |
| BauI | CACGAG | 1 cut(s) | 47 |
| Bbv12I | GWGCWC | 1 cut(s) | 48 |
| BccI | CCATC | 1 cut(s) | 185 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| BfuAI | ACCTGC | 1 cut(s) | 152 |
| BisI | GCNGC | 1 cut(s) | 11 |
| BlsI | GCNGC | 1 cut(s) | 12 |
| Bme18I | GGWCC | 1 cut(s) | 198 |
| BmgT120I | GGNCC | 1 cut(s) | 198 |
| BmiI | GGNNCC | 1 cut(s) | 166 |
| BpmI | CTGGAG | 1 cut(s) | 152 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 31 |
| Bse1I | ACTGG | 2 cut(s) | 21, 271 |
| BseGI | GGATG | 1 cut(s) | 177 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 31 |
| BseMII | CTCAG | 1 cut(s) | 92 |
| BseNI | ACTGG | 2 cut(s) | 21, 271 |
| BseYI | CCCAGC | 1 cut(s) | 148 |
| BshFI | GGCC | 2 cut(s) | 71, 92 |
| BshNI | GGYRCC | 1 cut(s) | 164 |
| BsiHKAI | GWGCWC | 1 cut(s) | 48 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 31 |
| BsmI | GAATGC | 1 cut(s) | 163 |
| BsnI | GGCC | 2 cut(s) | 71, 92 |
| Bsp1286I | GDGCHC | 1 cut(s) | 48 |
| BspACI | CCGC | 4 cut(s) | 168, 237, 260, 265 |
| BspANI | GGCC | 2 cut(s) | 71, 92 |
| BspCNI | CTCAG | 1 cut(s) | 91 |
| BspLI | GGNNCC | 1 cut(s) | 166 |
| BspMI | ACCTGC | 1 cut(s) | 152 |
| BspT107I | GGYRCC | 1 cut(s) | 164 |
| BsrBI | CCGCTC | 1 cut(s) | 262 |
| BsrI | ACTGG | 2 cut(s) | 21, 271 |
| BssSI | CACGAG | 1 cut(s) | 47 |
| Bst2BI | CACGAG | 1 cut(s) | 47 |
| BstDEI | CTNAG | 1 cut(s) | 78 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstF5I | GGATG | 1 cut(s) | 177 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 2 cut(s) | 130, 154 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 134, 149, 158, 167, 259 |
| BsuRI | GGCC | 2 cut(s) | 71, 92 |
| BtsCI | GGATG | 1 cut(s) | 177 |
| BtsIMutI | CAGTG | 2 cut(s) | 14, 278 |
| BveI | ACCTGC | 1 cut(s) | 152 |
| CfoI | GCGC | 2 cut(s) | 130, 154 |
| Cfr13I | GGNCC | 1 cut(s) | 198 |
| Csp6I | GTAC | 1 cut(s) | 165 |
| CviAII | CATG | 1 cut(s) | 250 |
| CviJI | RGCY | 6 cut(s) | 10, 71, 77, 92, 118, 185 |
| CviKI_1 | RGCY | 6 cut(s) | 10, 71, 77, 92, 118, 185 |
| CviQI | GTAC | 1 cut(s) | 165 |
| DdeI | CTNAG | 1 cut(s) | 78 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| Eco47I | GGWCC | 1 cut(s) | 198 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoO109I | RGGNCCY | 1 cut(s) | 198 |
| FaeI | CATG | 1 cut(s) | 253 |
| FaiI | YATR | 2 cut(s) | 6, 251 |
| FatI | CATG | 1 cut(s) | 249 |
| Fnu4HI | GCNGC | 1 cut(s) | 11 |
| FokI | GGATG | 1 cut(s) | 164 |
| Fsp4HI | GCNGC | 1 cut(s) | 11 |
| GlaI | GCGC | 2 cut(s) | 129, 153 |
| GluI | GCNGC | 1 cut(s) | 11 |
| GsaI | CCCAGC | 1 cut(s) | 152 |
| GsuI | CTGGAG | 1 cut(s) | 152 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 2 cut(s) | 71, 92 |
| HhaI | GCGC | 2 cut(s) | 130, 154 |
| Hin1II | CATG | 1 cut(s) | 253 |
| Hin6I | GCGC | 2 cut(s) | 128, 152 |
| HinP1I | GCGC | 2 cut(s) | 128, 152 |
| HinfI | GANTC | 1 cut(s) | 240 |
| Hpy188I | TCNGA | 2 cut(s) | 81, 182 |
| HpyAV | CCTTC | 2 cut(s) | 35, 118 |
| HpyCH4V | TGCA | 2 cut(s) | 161, 253 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 134, 149, 158, 167, 259 |
| HpyF3I | CTNAG | 1 cut(s) | 78 |
| Hsp92II | CATG | 1 cut(s) | 253 |
| HspAI | GCGC | 2 cut(s) | 128, 152 |
| KpnI | GGTACC | 1 cut(s) | 168 |
| LmnI | GCTCC | 1 cut(s) | 267 |
| LpnPI | CCDG | 5 cut(s) | 2, 46, 116, 147, 162 |
| MbiI | CCGCTC | 1 cut(s) | 262 |
| MhlI | GDGCHC | 1 cut(s) | 48 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MnlI | CCTC | 3 cut(s) | 49, 127, 211 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MslI | CAYNNNNRTG | 2 cut(s) | 98, 248 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspA1I | CMGCKG | 1 cut(s) | 170 |
| Mva1269I | GAATGC | 1 cut(s) | 163 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 134, 149, 158, 167, 259 |
| NlaIII | CATG | 1 cut(s) | 253 |
| NlaIV | GGNNCC | 1 cut(s) | 166 |
| PctI | GAATGC | 1 cut(s) | 163 |
| PfeI | GAWTC | 1 cut(s) | 240 |
| PkrI | GCNGC | 1 cut(s) | 12 |
| PpuMI | RGGWCCY | 1 cut(s) | 198 |
| Psp5II | RGGWCCY | 1 cut(s) | 198 |
| PspFI | CCCAGC | 1 cut(s) | 148 |
| PspN4I | GGNNCC | 1 cut(s) | 166 |
| PspPI | GGNCC | 1 cut(s) | 198 |
| PspPPI | RGGWCCY | 1 cut(s) | 198 |
| RsaI | GTAC | 1 cut(s) | 166 |
| RsaNI | GTAC | 1 cut(s) | 165 |
| RseI | CAYNNNNRTG | 2 cut(s) | 98, 248 |
| SatI | GCNGC | 1 cut(s) | 11 |
| Sau96I | GGNCC | 1 cut(s) | 198 |
| SduI | GDGCHC | 1 cut(s) | 48 |
| SetI | ASST | 7 cut(s) | 12, 27, 79, 120, 166, 187, 200 |
| SinI | GGWCC | 1 cut(s) | 198 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 98, 248 |
| SsiI | CCGC | 4 cut(s) | 168, 237, 260, 265 |
| TaqI | TCGA | 1 cut(s) | 111 |
| TfiI | GAWTC | 1 cut(s) | 240 |
| TscAI | CASTG | 2 cut(s) | 21, 278 |
| TseI | GCWGC | 1 cut(s) | 10 |
| TspRI | CASTG | 2 cut(s) | 21, 278 |
| VpaK11BI | GGWCC | 1 cut(s) | 198 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |