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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 201 |
| AciI | CCGC | 5 cut(s) | 92, 167, 185, 201, 254 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AcuI | CTGAAG | 1 cut(s) | 165 |
| AfaI | GTAC | 1 cut(s) | 219 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| AgeI | ACCGGT | 1 cut(s) | 214 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 163, 195 |
| AluBI | AGCT | 3 cut(s) | 207, 233, 244 |
| AluI | AGCT | 3 cut(s) | 207, 233, 244 |
| Alw21I | GWGCWC | 1 cut(s) | 294 |
| AoxI | GGCC | 2 cut(s) | 303, 309 |
| ApeKI | GCWGC | 3 cut(s) | 204, 207, 230 |
| AsiGI | ACCGGT | 1 cut(s) | 214 |
| AspS9I | GGNCC | 4 cut(s) | 31, 223, 256, 310 |
| AvaII | GGWCC | 3 cut(s) | 31, 223, 256 |
| Bbv12I | GWGCWC | 1 cut(s) | 294 |
| BbvI | GCAGC | 3 cut(s) | 194, 216, 242 |
| BccI | CCATC | 1 cut(s) | 66 |
| BisI | GCNGC | 4 cut(s) | 202, 205, 208, 231 |
| BlsI | GCNGC | 4 cut(s) | 203, 206, 209, 232 |
| Bme18I | GGWCC | 3 cut(s) | 31, 223, 256 |
| BmgT120I | GGNCC | 4 cut(s) | 31, 223, 256, 310 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BsaJI | CCNNGG | 1 cut(s) | 306 |
| BsaWI | WCCGGW | 1 cut(s) | 214 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| Bse118I | RCCGGY | 1 cut(s) | 214 |
| Bse1I | ACTGG | 1 cut(s) | 255 |
| BseDI | CCNNGG | 1 cut(s) | 306 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseMII | CTCAG | 1 cut(s) | 173 |
| BseNI | ACTGG | 1 cut(s) | 255 |
| BseRI | GAGGAG | 1 cut(s) | 147 |
| BseXI | GCAGC | 3 cut(s) | 194, 216, 242 |
| BshFI | GGCC | 2 cut(s) | 305, 311 |
| BshTI | ACCGGT | 1 cut(s) | 214 |
| BsiHKAI | GWGCWC | 1 cut(s) | 294 |
| BsiSI | CCGG | 1 cut(s) | 215 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BsnI | GGCC | 2 cut(s) | 305, 311 |
| Bsp1286I | GDGCHC | 1 cut(s) | 294 |
| Bsp143I | GATC | 3 cut(s) | 40, 75, 126 |
| BspACI | CCGC | 5 cut(s) | 92, 167, 185, 201, 254 |
| BspANI | GGCC | 2 cut(s) | 305, 311 |
| BspCNI | CTCAG | 1 cut(s) | 172 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BsrBI | CCGCTC | 1 cut(s) | 201 |
| BsrFI | RCCGGY | 1 cut(s) | 214 |
| BsrI | ACTGG | 1 cut(s) | 255 |
| BssAI | RCCGGY | 1 cut(s) | 214 |
| BssECI | CCNNGG | 1 cut(s) | 306 |
| BssMI | GATC | 3 cut(s) | 40, 75, 126 |
| Bst4CI | ACNGT | 1 cut(s) | 114 |
| BstC8I | GCNNGC | 1 cut(s) | 246 |
| BstDEI | CTNAG | 2 cut(s) | 159, 234 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstKTI | GATC | 3 cut(s) | 43, 78, 129 |
| BstMBI | GATC | 3 cut(s) | 40, 75, 126 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 207 |
| BstNSI | RCATGY | 1 cut(s) | 124 |
| BstV1I | GCAGC | 3 cut(s) | 194, 216, 242 |
| BsuRI | GGCC | 2 cut(s) | 305, 311 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 2 cut(s) | 110, 248 |
| Cac8I | GCNNGC | 1 cut(s) | 246 |
| Cfr10I | RCCGGY | 1 cut(s) | 214 |
| Cfr13I | GGNCC | 4 cut(s) | 31, 223, 256, 310 |
| Csp6I | GTAC | 1 cut(s) | 218 |
| CspAI | ACCGGT | 1 cut(s) | 214 |
| CviAII | CATG | 2 cut(s) | 107, 121 |
| CviJI | RGCY | 6 cut(s) | 191, 207, 233, 244, 305, 311 |
| CviKI_1 | RGCY | 6 cut(s) | 191, 207, 233, 244, 305, 311 |
| CviQI | GTAC | 1 cut(s) | 218 |
| DdeI | CTNAG | 2 cut(s) | 159, 234 |
| DpnI | GATC | 3 cut(s) | 42, 77, 128 |
| DpnII | GATC | 3 cut(s) | 40, 75, 126 |
| EciI | GGCGGA | 2 cut(s) | 156, 269 |
| Eco47I | GGWCC | 3 cut(s) | 31, 223, 256 |
| Eco57I | CTGAAG | 1 cut(s) | 165 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| FaeI | CATG | 2 cut(s) | 110, 124 |
| FaiI | YATR | 4 cut(s) | 108, 122, 240, 296 |
| FatI | CATG | 2 cut(s) | 106, 120 |
| Fnu4HI | GCNGC | 4 cut(s) | 202, 205, 208, 231 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 4 cut(s) | 202, 205, 208, 231 |
| GluI | GCNGC | 4 cut(s) | 202, 205, 208, 231 |
| HaeIII | GGCC | 2 cut(s) | 305, 311 |
| HapII | CCGG | 1 cut(s) | 215 |
| Hin1II | CATG | 2 cut(s) | 110, 124 |
| HincII | GTYRAC | 2 cut(s) | 97, 103 |
| HindII | GTYRAC | 2 cut(s) | 97, 103 |
| HinfI | GANTC | 1 cut(s) | 163 |
| HpaI | GTTAAC | 2 cut(s) | 97, 103 |
| HpaII | CCGG | 1 cut(s) | 215 |
| Hpy166II | GTNNAC | 2 cut(s) | 97, 103 |
| Hpy188I | TCNGA | 1 cut(s) | 162 |
| Hpy8I | GTNNAC | 2 cut(s) | 97, 103 |
| HpyAV | CCTTC | 1 cut(s) | 269 |
| HpyCH4III | ACNGT | 1 cut(s) | 114 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 1 cut(s) | 110 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 207 |
| HpyF3I | CTNAG | 2 cut(s) | 159, 234 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| Hsp92II | CATG | 2 cut(s) | 110, 124 |
| KspAI | GTTAAC | 2 cut(s) | 97, 103 |
| Kzo9I | GATC | 3 cut(s) | 40, 75, 126 |
| LmnI | GCTCC | 1 cut(s) | 289 |
| LpnPI | CCDG | 6 cut(s) | 102, 206, 228, 230, 236, 272 |
| Lsp1109I | GCAGC | 3 cut(s) | 194, 216, 242 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MalI | GATC | 3 cut(s) | 42, 77, 128 |
| MbiI | CCGCTC | 1 cut(s) | 201 |
| MboI | GATC | 3 cut(s) | 40, 75, 126 |
| MhlI | GDGCHC | 1 cut(s) | 294 |
| MlyI | GAGTC | 1 cut(s) | 157 |
| MnlI | CCTC | 5 cut(s) | 22, 168, 181, 236, 301 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MspA1I | CMGCKG | 3 cut(s) | 92, 185, 207 |
| MspI | CCGG | 1 cut(s) | 215 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 207 |
| NdeII | GATC | 3 cut(s) | 40, 75, 126 |
| NlaIII | CATG | 2 cut(s) | 110, 124 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NspI | RCATGY | 1 cut(s) | 124 |
| PinAI | ACCGGT | 1 cut(s) | 214 |
| PkrI | GCNGC | 4 cut(s) | 203, 206, 209, 232 |
| PleI | GAGTC | 1 cut(s) | 157 |
| PpsI | GAGTC | 1 cut(s) | 157 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 4 cut(s) | 31, 223, 256, 310 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| PvuII | CAGCTG | 1 cut(s) | 207 |
| RsaI | GTAC | 1 cut(s) | 219 |
| RsaNI | GTAC | 1 cut(s) | 218 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 4 cut(s) | 202, 205, 208, 231 |
| Sau3AI | GATC | 3 cut(s) | 40, 75, 126 |
| Sau96I | GGNCC | 4 cut(s) | 31, 223, 256, 310 |
| SchI | GAGTC | 1 cut(s) | 157 |
| SduI | GDGCHC | 1 cut(s) | 294 |
| SetI | ASST | 7 cut(s) | 84, 102, 209, 228, 235, 246, 261 |
| SinI | GGWCC | 3 cut(s) | 31, 223, 256 |
| SsiI | CCGC | 5 cut(s) | 92, 167, 185, 201, 254 |
| TaaI | ACNGT | 1 cut(s) | 114 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 1 cut(s) | 27 |
| TauI | GCSGC | 1 cut(s) | 204 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TscAI | CASTG | 2 cut(s) | 117, 255 |
| TseI | GCWGC | 3 cut(s) | 204, 207, 230 |
| TspRI | CASTG | 2 cut(s) | 117, 255 |
| VpaK11BI | GGWCC | 3 cut(s) | 31, 223, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XceI | RCATGY | 1 cut(s) | 124 |