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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 457 |
| Acc65I | GGTACC | 2 cut(s) | 442, 469 |
| AccB1I | GGYRCC | 2 cut(s) | 442, 469 |
| AccII | CGCG | 1 cut(s) | 282 |
| AciI | CCGC | 8 cut(s) | 15, 38, 48, 92, 138, 185, 208, 323 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcuI | CTGAAG | 1 cut(s) | 374 |
| AdeI | CACNNNGTG | 1 cut(s) | 414 |
| AfaI | GTAC | 3 cut(s) | 444, 459, 471 |
| AfeI | AGCGCT | 1 cut(s) | 387 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 139, 214, 265 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 2 cut(s) | 147, 410 |
| AluBI | AGCT | 3 cut(s) | 244, 352, 490 |
| AluI | AGCT | 3 cut(s) | 244, 352, 490 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 410 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 1 cut(s) | 48 |
| Aor51HI | AGCGCT | 1 cut(s) | 387 |
| AoxI | GGCC | 2 cut(s) | 303, 324 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| Asp718I | GGTACC | 2 cut(s) | 442, 469 |
| AspLEI | GCGC | 3 cut(s) | 282, 364, 388 |
| AspS9I | GGNCC | 3 cut(s) | 223, 449, 503 |
| AsuC2I | CCSGG | 1 cut(s) | 447 |
| AvaII | GGWCC | 3 cut(s) | 223, 449, 503 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BanI | GGYRCC | 2 cut(s) | 442, 469 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 410 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BciT130I | CCWGG | 2 cut(s) | 149, 412 |
| BcnI | CCSGG | 1 cut(s) | 447 |
| BfmI | CTRYAG | 1 cut(s) | 180 |
| BfoI | RGCGCY | 2 cut(s) | 365, 389 |
| BfuAI | ACCTGC | 1 cut(s) | 457 |
| BisI | GCNGC | 2 cut(s) | 208, 324 |
| BlpI | GCTNAGC | 2 cut(s) | 192, 389 |
| BlsI | GCNGC | 2 cut(s) | 209, 325 |
| Bme1390I | CCNGG | 3 cut(s) | 149, 412, 447 |
| Bme18I | GGWCC | 3 cut(s) | 223, 449, 503 |
| BmgT120I | GGNCC | 3 cut(s) | 223, 449, 503 |
| BmiI | GGNNCC | 3 cut(s) | 444, 471, 504 |
| BmrFI | CCNGG | 3 cut(s) | 149, 412, 447 |
| BmsI | GCATC | 1 cut(s) | 453 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 192, 389 |
| BpuMI | CCSGG | 1 cut(s) | 447 |
| BsaJI | CCNNGG | 2 cut(s) | 147, 306 |
| BsaWI | WCCGGW | 1 cut(s) | 214 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 139, 214, 265 |
| Bse118I | RCCGGY | 1 cut(s) | 124 |
| BseBI | CCWGG | 2 cut(s) | 149, 412 |
| BseDI | CCNNGG | 2 cut(s) | 147, 306 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 139, 214, 265 |
| BseMII | CTCAG | 2 cut(s) | 18, 206 |
| BseRI | GAGGAG | 2 cut(s) | 44, 220 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseYI | CCCAGC | 2 cut(s) | 134, 382 |
| BsgI | GTGCAG | 2 cut(s) | 239, 436 |
| Bsh1236I | CGCG | 1 cut(s) | 282 |
| BshFI | GGCC | 2 cut(s) | 305, 326 |
| BshNI | GGYRCC | 2 cut(s) | 442, 469 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 410 |
| BsiSI | CCGG | 3 cut(s) | 125, 215, 447 |
| BslFI | GGGAC | 1 cut(s) | 516 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 139, 214, 265 |
| BsmFI | GGGAC | 1 cut(s) | 516 |
| BsnI | GGCC | 2 cut(s) | 305, 326 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 410 |
| Bsp1407I | TGTACA | 1 cut(s) | 457 |
| Bsp143I | GATC | 2 cut(s) | 40, 75 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 192, 389 |
| BspACI | CCGC | 8 cut(s) | 15, 38, 48, 92, 138, 185, 208, 323 |
| BspANI | GGCC | 2 cut(s) | 305, 326 |
| BspCNI | CTCAG | 2 cut(s) | 19, 205 |
| BspFNI | CGCG | 1 cut(s) | 282 |
| BspLI | GGNNCC | 3 cut(s) | 444, 471, 504 |
| BspMI | ACCTGC | 1 cut(s) | 457 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BspT107I | GGYRCC | 2 cut(s) | 442, 469 |
| BsrFI | RCCGGY | 1 cut(s) | 124 |
| BsrGI | TGTACA | 1 cut(s) | 457 |
| BssAI | RCCGGY | 1 cut(s) | 124 |
| BssECI | CCNNGG | 2 cut(s) | 147, 306 |
| BssMI | GATC | 2 cut(s) | 40, 75 |
| Bst2UI | CCWGG | 2 cut(s) | 149, 412 |
| Bst4CI | ACNGT | 2 cut(s) | 107, 474 |
| Bst6I | CTCTTC | 1 cut(s) | 350 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 414 |
| BstAUI | TGTACA | 1 cut(s) | 457 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 246, 284, 321, 395 |
| BstDEI | CTNAG | 3 cut(s) | 27, 192, 389 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstFNI | CGCG | 1 cut(s) | 282 |
| BstH2I | RGCGCY | 2 cut(s) | 365, 389 |
| BstHHI | GCGC | 3 cut(s) | 282, 364, 388 |
| BstKTI | GATC | 2 cut(s) | 43, 78 |
| BstMBI | GATC | 2 cut(s) | 40, 75 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 207, 368, 383, 394, 414 |
| BstNI | CCWGG | 2 cut(s) | 149, 412 |
| BstSCI | CCNGG | 3 cut(s) | 147, 410, 445 |
| BstSFI | CTRYAG | 1 cut(s) | 180 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 1 cut(s) | 282 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 439 |
| BsuRI | GGCC | 2 cut(s) | 305, 326 |
| BveI | ACCTGC | 1 cut(s) | 457 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 246, 284, 321, 395 |
| CfoI | GCGC | 3 cut(s) | 282, 364, 388 |
| Cfr10I | RCCGGY | 1 cut(s) | 124 |
| Cfr13I | GGNCC | 3 cut(s) | 223, 449, 503 |
| CsiI | ACCWGGT | 1 cut(s) | 410 |
| Csp6I | GTAC | 3 cut(s) | 443, 458, 470 |
| CviJI | RGCY | 8 cut(s) | 191, 196, 207, 244, 305, 326, 352, 490 |
| CviKI_1 | RGCY | 8 cut(s) | 191, 196, 207, 244, 305, 326, 352, 490 |
| CviQI | GTAC | 3 cut(s) | 443, 458, 470 |
| DdeI | CTNAG | 3 cut(s) | 27, 192, 389 |
| DpnI | GATC | 2 cut(s) | 42, 77 |
| DpnII | GATC | 2 cut(s) | 40, 75 |
| DraIII | CACNNNGTG | 1 cut(s) | 414 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| Eam1104I | CTCTTC | 1 cut(s) | 350 |
| EarI | CTCTTC | 1 cut(s) | 350 |
| EciI | GGCGGA | 1 cut(s) | 37 |
| Eco47I | GGWCC | 3 cut(s) | 223, 449, 503 |
| Eco47III | AGCGCT | 1 cut(s) | 387 |
| Eco57I | CTGAAG | 1 cut(s) | 374 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| EcoRII | CCWGG | 2 cut(s) | 147, 410 |
| FaiI | YATR | 2 cut(s) | 236, 240 |
| FaqI | GGGAC | 1 cut(s) | 516 |
| FauI | CCCGC | 2 cut(s) | 8, 131 |
| Fnu4HI | GCNGC | 2 cut(s) | 208, 324 |
| Fsp4HI | GCNGC | 2 cut(s) | 208, 324 |
| GlaI | GCGC | 3 cut(s) | 281, 363, 387 |
| GluI | GCNGC | 2 cut(s) | 208, 324 |
| GsaI | CCCAGC | 2 cut(s) | 138, 386 |
| HaeII | RGCGCY | 2 cut(s) | 365, 389 |
| HaeIII | GGCC | 2 cut(s) | 305, 326 |
| HapII | CCGG | 3 cut(s) | 125, 215, 447 |
| HhaI | GCGC | 3 cut(s) | 282, 364, 388 |
| Hin6I | GCGC | 3 cut(s) | 280, 362, 386 |
| HinP1I | GCGC | 3 cut(s) | 280, 362, 386 |
| HincII | GTYRAC | 3 cut(s) | 103, 130, 422 |
| HindII | GTYRAC | 3 cut(s) | 103, 130, 422 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 3 cut(s) | 125, 215, 447 |
| Hpy166II | GTNNAC | 4 cut(s) | 103, 110, 130, 422 |
| Hpy188III | TCNNGA | 2 cut(s) | 275, 512 |
| Hpy8I | GTNNAC | 4 cut(s) | 103, 110, 130, 422 |
| HpyAV | CCTTC | 1 cut(s) | 269 |
| HpyCH4III | ACNGT | 2 cut(s) | 107, 474 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 4 cut(s) | 110, 220, 417, 466 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 207, 368, 383, 394, 414 |
| HpyF3I | CTNAG | 3 cut(s) | 27, 192, 389 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| HspAI | GCGC | 3 cut(s) | 280, 362, 386 |
| KpnI | GGTACC | 2 cut(s) | 446, 473 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 75 |
| LmnI | GCTCC | 1 cut(s) | 31 |
| LweI | GCATC | 1 cut(s) | 453 |
| MabI | ACCWGGT | 1 cut(s) | 410 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MaeIII | GTNAC | 1 cut(s) | 155 |
| MalI | GATC | 2 cut(s) | 42, 77 |
| MboI | GATC | 2 cut(s) | 40, 75 |
| MboII | GAAGA | 1 cut(s) | 367 |
| MfeI | CAATTG | 1 cut(s) | 453 |
| MhlI | GDGCHC | 2 cut(s) | 112, 410 |
| MluCI | AATT | 1 cut(s) | 453 |
| MmeI | TCCRAC | 1 cut(s) | 510 |
| MnlI | CCTC | 9 cut(s) | 22, 181, 222, 236, 241, 301, 351, 488, 519 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 476 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| MspA1I | CMGCKG | 3 cut(s) | 92, 138, 185 |
| MspI | CCGG | 3 cut(s) | 125, 215, 447 |
| MspR9I | CCNGG | 3 cut(s) | 149, 412, 447 |
| MunI | CAATTG | 1 cut(s) | 453 |
| MvaI | CCWGG | 2 cut(s) | 149, 412 |
| MvnI | CGCG | 1 cut(s) | 282 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 207, 368, 383, 394, 414 |
| NciI | CCSGG | 1 cut(s) | 447 |
| NdeII | GATC | 2 cut(s) | 40, 75 |
| NlaIV | GGNNCC | 3 cut(s) | 444, 471, 504 |
| NmeAIII | GCCGAG | 2 cut(s) | 222, 331 |
| PkrI | GCNGC | 2 cut(s) | 209, 325 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| Psp6I | CCWGG | 2 cut(s) | 147, 410 |
| PspFI | CCCAGC | 2 cut(s) | 134, 382 |
| PspGI | CCWGG | 2 cut(s) | 147, 410 |
| PspN4I | GGNNCC | 3 cut(s) | 444, 471, 504 |
| PspPI | GGNCC | 3 cut(s) | 223, 449, 503 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| RsaI | GTAC | 3 cut(s) | 444, 459, 471 |
| RsaNI | GTAC | 3 cut(s) | 443, 458, 470 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 476 |
| SatI | GCNGC | 2 cut(s) | 208, 324 |
| Sau3AI | GATC | 2 cut(s) | 40, 75 |
| Sau96I | GGNCC | 3 cut(s) | 223, 449, 503 |
| ScrFI | CCNGG | 3 cut(s) | 149, 412, 447 |
| SduI | GDGCHC | 2 cut(s) | 112, 410 |
| SetI | ASST | 9 cut(s) | 84, 228, 246, 261, 354, 416, 471, 492, 511 |
| SexAI | ACCWGGT | 1 cut(s) | 410 |
| SfaNI | GCATC | 1 cut(s) | 453 |
| SfcI | CTRYAG | 1 cut(s) | 180 |
| SinI | GGWCC | 3 cut(s) | 223, 449, 503 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| Sse9I | AATT | 1 cut(s) | 453 |
| SsiI | CCGC | 8 cut(s) | 15, 38, 48, 92, 138, 185, 208, 323 |
| StyD4I | CCNGG | 3 cut(s) | 147, 410, 445 |
| TaaI | ACNGT | 2 cut(s) | 107, 474 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TaqI | TCGA | 1 cut(s) | 274 |
| TasI | AATT | 1 cut(s) | 453 |
| TatI | WGTACW | 1 cut(s) | 457 |
| TauI | GCSGC | 2 cut(s) | 210, 326 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 476 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 476 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 3 cut(s) | 223, 449, 503 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |