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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 463 |
| Acc36I | ACCTGC | 1 cut(s) | 463 |
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 2 cut(s) | 74, 90 |
| AclWI | GGATC | 2 cut(s) | 201, 527 |
| AcoI | YGGCCR | 2 cut(s) | 108, 306 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 3 cut(s) | 176, 396, 408 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 519, 546 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 5 cut(s) | 133, 347, 382, 421, 512 |
| AlwI | GGATC | 2 cut(s) | 201, 527 |
| AoxI | GGCC | 5 cut(s) | 108, 154, 240, 306, 386 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 263 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 6 cut(s) | 92, 231, 268, 368, 374, 552 |
| AspS9I | GGNCC | 2 cut(s) | 386, 440 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 320 |
| AsuHPI | GGTGA | 3 cut(s) | 134, 160, 542 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 250 |
| BccI | CCATC | 1 cut(s) | 253 |
| BciT130I | CCWGG | 5 cut(s) | 135, 349, 384, 423, 514 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BfaI | CTAG | 1 cut(s) | 546 |
| BfoI | RGCGCY | 1 cut(s) | 369 |
| BfuAI | ACCTGC | 1 cut(s) | 463 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 446 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 6 cut(s) | 140, 143, 146, 265, 307, 447 |
| Bme1390I | CCNGG | 8 cut(s) | 107, 117, 135, 320, 349, 384, 423, 514 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmgT120I | GGNCC | 2 cut(s) | 386, 440 |
| BmiI | GGNNCC | 2 cut(s) | 381, 441 |
| BmrFI | CCNGG | 8 cut(s) | 107, 117, 135, 320, 349, 384, 423, 514 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 320 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 115, 383, 422, 513, 545 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 368, 519, 546 |
| Bse118I | RCCGGY | 2 cut(s) | 368, 388 |
| Bse3DI | GCAATG | 1 cut(s) | 381 |
| BseBI | CCWGG | 5 cut(s) | 135, 349, 384, 423, 514 |
| BseDI | CCNNGG | 6 cut(s) | 105, 115, 383, 422, 513, 545 |
| BseGI | GGATG | 1 cut(s) | 409 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 519, 546 |
| BseMI | GCAATG | 1 cut(s) | 381 |
| BseMII | CTCAG | 1 cut(s) | 516 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 250 |
| BseYI | CCCAGC | 1 cut(s) | 362 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 90 |
| Bsh1285I | CGRYCG | 3 cut(s) | 78, 316, 393 |
| BshFI | GGCC | 5 cut(s) | 110, 156, 242, 308, 388 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 3 cut(s) | 78, 316, 393 |
| BsiSI | CCGG | 5 cut(s) | 107, 117, 320, 369, 389 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 368, 519, 546 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| BsnI | GGCC | 5 cut(s) | 110, 156, 242, 308, 388 |
| Bsp143I | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BspANI | GGCC | 5 cut(s) | 110, 156, 242, 308, 388 |
| BspCNI | CTCAG | 1 cut(s) | 517 |
| BspFNI | CGCG | 2 cut(s) | 74, 90 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 2 cut(s) | 381, 441 |
| BspMI | ACCTGC | 1 cut(s) | 463 |
| BspPI | GGATC | 2 cut(s) | 201, 527 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrDI | GCAATG | 1 cut(s) | 381 |
| BsrFI | RCCGGY | 2 cut(s) | 368, 388 |
| BssAI | RCCGGY | 2 cut(s) | 368, 388 |
| BssECI | CCNNGG | 6 cut(s) | 105, 115, 383, 422, 513, 545 |
| BssMI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 5 cut(s) | 135, 349, 384, 423, 514 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 332, 370, 554 |
| BstDEI | CTNAG | 1 cut(s) | 525 |
| BstF5I | GGATG | 1 cut(s) | 409 |
| BstFNI | CGCG | 2 cut(s) | 74, 90 |
| BstH2I | RGCGCY | 1 cut(s) | 369 |
| BstHHI | GCGC | 6 cut(s) | 92, 231, 268, 368, 374, 552 |
| BstKTI | GATC | 4 cut(s) | 78, 163, 196, 535 |
| BstMBI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BstMCI | CGRYCG | 3 cut(s) | 78, 316, 393 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 87, 144, 153 |
| BstNI | CCWGG | 5 cut(s) | 135, 349, 384, 423, 514 |
| BstSCI | CCNGG | 8 cut(s) | 105, 115, 133, 318, 347, 382, 421, 512 |
| BstUI | CGCG | 2 cut(s) | 74, 90 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 250 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 5 cut(s) | 110, 156, 242, 308, 388 |
| BtsCI | GGATG | 1 cut(s) | 409 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 463 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 332, 370, 554 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 6 cut(s) | 92, 231, 268, 368, 374, 552 |
| Cfr10I | RCCGGY | 2 cut(s) | 368, 388 |
| Cfr13I | GGNCC | 2 cut(s) | 386, 440 |
| Csp6I | GTAC | 3 cut(s) | 175, 395, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 2 cut(s) | 172, 271 |
| CviJI | RGCY | 9 cut(s) | 110, 144, 156, 242, 248, 263, 308, 388, 556 |
| CviKI_1 | RGCY | 9 cut(s) | 110, 144, 156, 242, 248, 263, 308, 388, 556 |
| CviQI | GTAC | 3 cut(s) | 175, 395, 407 |
| DdeI | CTNAG | 1 cut(s) | 525 |
| DpnI | GATC | 4 cut(s) | 77, 162, 195, 534 |
| DpnII | GATC | 4 cut(s) | 75, 160, 193, 532 |
| EaeI | YGGCCR | 2 cut(s) | 108, 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 525 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoRII | CCWGG | 5 cut(s) | 133, 347, 382, 421, 512 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| FaeI | CATG | 2 cut(s) | 175, 274 |
| FaiI | YATR | 5 cut(s) | 173, 201, 272, 452, 542 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FatI | CATG | 2 cut(s) | 171, 270 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 446 |
| FokI | GGATG | 1 cut(s) | 416 |
| Fsp4HI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 446 |
| FspBI | CTAG | 1 cut(s) | 546 |
| GlaI | GCGC | 6 cut(s) | 91, 230, 267, 367, 373, 551 |
| GluI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 446 |
| GsaI | CCCAGC | 1 cut(s) | 366 |
| HaeII | RGCGCY | 1 cut(s) | 369 |
| HaeIII | GGCC | 5 cut(s) | 110, 156, 242, 308, 388 |
| HapII | CCGG | 5 cut(s) | 107, 117, 320, 369, 389 |
| HhaI | GCGC | 6 cut(s) | 92, 231, 268, 368, 374, 552 |
| Hin1II | CATG | 2 cut(s) | 175, 274 |
| Hin6I | GCGC | 6 cut(s) | 90, 229, 266, 366, 372, 550 |
| HinP1I | GCGC | 6 cut(s) | 90, 229, 266, 366, 372, 550 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 5 cut(s) | 107, 117, 320, 369, 389 |
| HphI | GGTGA | 3 cut(s) | 134, 160, 542 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 2 cut(s) | 271, 432 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4IV | ACGT | 2 cut(s) | 277, 340 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 87, 144, 153 |
| HpyF3I | CTNAG | 1 cut(s) | 525 |
| HpySE526I | ACGT | 2 cut(s) | 277, 340 |
| Hsp92II | CATG | 2 cut(s) | 175, 274 |
| HspAI | GCGC | 6 cut(s) | 90, 229, 266, 366, 372, 550 |
| KroI | GCCGGC | 1 cut(s) | 368 |
| KroNI | GCCGGC | 1 cut(s) | 370 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 4 cut(s) | 75, 160, 193, 532 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 250 |
| MaeI | CTAG | 1 cut(s) | 546 |
| MaeII | ACGT | 2 cut(s) | 277, 340 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 4 cut(s) | 77, 162, 195, 534 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MnlI | CCTC | 7 cut(s) | 73, 118, 214, 217, 277, 465, 520 |
| MreI | CGCCGGCG | 1 cut(s) | 368 |
| MroNI | GCCGGC | 1 cut(s) | 368 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 1 cut(s) | 412 |
| MspI | CCGG | 5 cut(s) | 107, 117, 320, 369, 389 |
| MspR9I | CCNGG | 8 cut(s) | 107, 117, 135, 320, 349, 384, 423, 514 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 5 cut(s) | 135, 349, 384, 423, 514 |
| MvnI | CGCG | 2 cut(s) | 74, 90 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 87, 144, 153 |
| NaeI | GCCGGC | 1 cut(s) | 370 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 320 |
| NdeII | GATC | 4 cut(s) | 75, 160, 193, 532 |
| NgoMIV | GCCGGC | 1 cut(s) | 368 |
| NlaIII | CATG | 2 cut(s) | 175, 274 |
| NlaIV | GGNNCC | 2 cut(s) | 381, 441 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PaqCI | CACCTGC | 1 cut(s) | 463 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdiI | GCCGGC | 1 cut(s) | 370 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 1 cut(s) | 482 |
| PkrI | GCNGC | 6 cut(s) | 140, 143, 146, 265, 307, 447 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| Psp6I | CCWGG | 5 cut(s) | 133, 347, 382, 421, 512 |
| PspFI | CCCAGC | 1 cut(s) | 362 |
| PspGI | CCWGG | 5 cut(s) | 133, 347, 382, 421, 512 |
| PspN4I | GGNNCC | 2 cut(s) | 381, 441 |
| PspPI | GGNCC | 2 cut(s) | 386, 440 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 3 cut(s) | 176, 396, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 395, 407 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 306, 446 |
| Sau3AI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| Sau96I | GGNCC | 2 cut(s) | 386, 440 |
| ScrFI | CCNGG | 8 cut(s) | 107, 117, 135, 320, 349, 384, 423, 514 |
| SetI | ASST | 6 cut(s) | 280, 343, 424, 457, 477, 547 |
| SgrAI | CRCCGGYG | 1 cut(s) | 368 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SspMI | CTAG | 1 cut(s) | 546 |
| StyD4I | CCNGG | 8 cut(s) | 105, 115, 133, 318, 347, 382, 421, 512 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaiI | ACGT | 2 cut(s) | 280, 343 |
| TaqI | TCGA | 2 cut(s) | 358, 506 |
| TauI | GCSGC | 3 cut(s) | 147, 308, 448 |
| TfiI | GAWTC | 1 cut(s) | 482 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 263 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspGWI | ACGGA | 1 cut(s) | 206 |
| TspRI | CASTG | 1 cut(s) | 192 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 1 cut(s) | 546 |