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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 322 |
| AccII | CGCG | 1 cut(s) | 367 |
| AciI | CCGC | 4 cut(s) | 15, 48, 208, 219 |
| AclWI | GGATC | 1 cut(s) | 219 |
| AcoI | YGGCCR | 1 cut(s) | 216 |
| AcsI | RAATTY | 1 cut(s) | 329 |
| AfeI | AGCGCT | 1 cut(s) | 351 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 416, 470 |
| AluBI | AGCT | 2 cut(s) | 299, 340 |
| AluI | AGCT | 2 cut(s) | 299, 340 |
| Alw26I | GTCTC | 1 cut(s) | 464 |
| AlwI | GGATC | 1 cut(s) | 219 |
| Ama87I | CYCGRG | 1 cut(s) | 143 |
| Aor51HI | AGCGCT | 1 cut(s) | 351 |
| AoxI | GGCC | 2 cut(s) | 216, 401 |
| ApoI | RAATTY | 1 cut(s) | 329 |
| AspLEI | GCGC | 3 cut(s) | 202, 352, 367 |
| AspS9I | GGNCC | 1 cut(s) | 402 |
| AsuHPI | GGTGA | 2 cut(s) | 69, 94 |
| AsuNHI | GCTAGC | 1 cut(s) | 5 |
| AvaI | CYCGRG | 1 cut(s) | 143 |
| BbvCI | CCTCAGC | 1 cut(s) | 57 |
| BccI | CCATC | 1 cut(s) | 201 |
| BcoDI | GTCTC | 1 cut(s) | 464 |
| BfaI | CTAG | 2 cut(s) | 6, 353 |
| BfoI | RGCGCY | 1 cut(s) | 353 |
| BisI | GCNGC | 3 cut(s) | 16, 48, 219 |
| BlsI | GCNGC | 3 cut(s) | 17, 49, 220 |
| BmeT110I | CYCGRG | 1 cut(s) | 143 |
| BmgT120I | GGNCC | 1 cut(s) | 402 |
| BmtI | GCTAGC | 1 cut(s) | 9 |
| Bpu10I | CCTNAGC | 1 cut(s) | 57 |
| BsaJI | CCNNGG | 2 cut(s) | 144, 237 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 416, 470 |
| Bse118I | RCCGGY | 1 cut(s) | 182 |
| BseDI | CCNNGG | 2 cut(s) | 144, 237 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 416, 470 |
| BseMII | CTCAG | 1 cut(s) | 71 |
| BseYI | CCCAGC | 1 cut(s) | 404 |
| Bsh1236I | CGCG | 1 cut(s) | 367 |
| BshFI | GGCC | 2 cut(s) | 218, 403 |
| BsiHKCI | CYCGRG | 1 cut(s) | 143 |
| BsiSI | CCGG | 1 cut(s) | 183 |
| BslFI | GGGAC | 1 cut(s) | 433 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 416, 470 |
| BsmAI | GTCTC | 1 cut(s) | 464 |
| BsmFI | GGGAC | 1 cut(s) | 433 |
| BsnI | GGCC | 2 cut(s) | 218, 403 |
| BsoBI | CYCGRG | 1 cut(s) | 143 |
| Bsp143I | GATC | 2 cut(s) | 40, 211 |
| BspACI | CCGC | 4 cut(s) | 15, 48, 208, 219 |
| BspANI | GGCC | 2 cut(s) | 218, 403 |
| BspCNI | CTCAG | 1 cut(s) | 70 |
| BspFNI | CGCG | 1 cut(s) | 367 |
| BspOI | GCTAGC | 1 cut(s) | 9 |
| BspPI | GGATC | 1 cut(s) | 219 |
| BsrFI | RCCGGY | 1 cut(s) | 182 |
| BssAI | RCCGGY | 1 cut(s) | 182 |
| BssECI | CCNNGG | 2 cut(s) | 144, 237 |
| BssMI | GATC | 2 cut(s) | 40, 211 |
| BssT1I | CCWWGG | 1 cut(s) | 237 |
| Bst4CI | ACNGT | 3 cut(s) | 85, 92, 99 |
| BstC8I | GCNNGC | 1 cut(s) | 7 |
| BstDEI | CTNAG | 1 cut(s) | 57 |
| BstFNI | CGCG | 1 cut(s) | 367 |
| BstH2I | RGCGCY | 1 cut(s) | 353 |
| BstHHI | GCGC | 3 cut(s) | 202, 352, 367 |
| BstKTI | GATC | 2 cut(s) | 43, 214 |
| BstMAI | GTCTC | 1 cut(s) | 464 |
| BstMBI | GATC | 2 cut(s) | 40, 211 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 15, 21, 44 |
| BstUI | CGCG | 1 cut(s) | 367 |
| BstX2I | RGATCY | 1 cut(s) | 211 |
| BstYI | RGATCY | 1 cut(s) | 211 |
| BsuRI | GGCC | 2 cut(s) | 218, 403 |
| Cac8I | GCNNGC | 1 cut(s) | 7 |
| CfoI | GCGC | 3 cut(s) | 202, 352, 367 |
| Cfr10I | RCCGGY | 1 cut(s) | 182 |
| Cfr13I | GGNCC | 1 cut(s) | 402 |
| CseI | GACGC | 1 cut(s) | 325 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 99, 134 |
| CviAII | CATG | 1 cut(s) | 25 |
| DdeI | CTNAG | 1 cut(s) | 57 |
| DpnI | GATC | 2 cut(s) | 42, 213 |
| DpnII | GATC | 2 cut(s) | 40, 211 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 322 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 322 |
| EaeI | YGGCCR | 1 cut(s) | 216 |
| Eco130I | CCWWGG | 1 cut(s) | 237 |
| Eco47III | AGCGCT | 1 cut(s) | 351 |
| Eco88I | CYCGRG | 1 cut(s) | 143 |
| EcoT14I | CCWWGG | 1 cut(s) | 237 |
| ErhI | CCWWGG | 1 cut(s) | 237 |
| FaeI | CATG | 1 cut(s) | 28 |
| FaiI | YATR | 4 cut(s) | 26, 107, 224, 416 |
| FaqI | GGGAC | 1 cut(s) | 433 |
| FatI | CATG | 1 cut(s) | 24 |
| FauI | CCCGC | 1 cut(s) | 201 |
| Fnu4HI | GCNGC | 3 cut(s) | 16, 48, 219 |
| Fsp4HI | GCNGC | 3 cut(s) | 16, 48, 219 |
| FspBI | CTAG | 2 cut(s) | 6, 353 |
| GlaI | GCGC | 3 cut(s) | 201, 351, 366 |
| GluI | GCNGC | 3 cut(s) | 16, 48, 219 |
| GsaI | CCCAGC | 1 cut(s) | 408 |
| HaeII | RGCGCY | 1 cut(s) | 353 |
| HaeIII | GGCC | 2 cut(s) | 218, 403 |
| HapII | CCGG | 1 cut(s) | 183 |
| HgaI | GACGC | 1 cut(s) | 325 |
| HhaI | GCGC | 3 cut(s) | 202, 352, 367 |
| Hin1II | CATG | 1 cut(s) | 28 |
| Hin6I | GCGC | 3 cut(s) | 200, 350, 365 |
| HinP1I | GCGC | 3 cut(s) | 200, 350, 365 |
| HincII | GTYRAC | 1 cut(s) | 88 |
| HindII | GTYRAC | 1 cut(s) | 88 |
| HinfI | GANTC | 1 cut(s) | 130 |
| HpaII | CCGG | 1 cut(s) | 183 |
| HphI | GGTGA | 2 cut(s) | 69, 94 |
| Hpy166II | GTNNAC | 5 cut(s) | 88, 95, 102, 121, 383 |
| Hpy8I | GTNNAC | 5 cut(s) | 88, 95, 102, 121, 383 |
| Hpy99I | CGWCG | 1 cut(s) | 319 |
| HpyAV | CCTTC | 1 cut(s) | 283 |
| HpyCH4III | ACNGT | 3 cut(s) | 85, 92, 99 |
| HpyCH4IV | ACGT | 1 cut(s) | 117 |
| HpyCH4V | TGCA | 1 cut(s) | 398 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 15, 21, 44 |
| HpyF3I | CTNAG | 1 cut(s) | 57 |
| HpySE526I | ACGT | 1 cut(s) | 117 |
| Hsp92II | CATG | 1 cut(s) | 28 |
| HspAI | GCGC | 3 cut(s) | 200, 350, 365 |
| Kzo9I | GATC | 2 cut(s) | 40, 211 |
| LpnPI | CCDG | 4 cut(s) | 81, 196, 200, 418 |
| MaeI | CTAG | 2 cut(s) | 6, 353 |
| MaeII | ACGT | 1 cut(s) | 117 |
| MalI | GATC | 2 cut(s) | 42, 213 |
| MboI | GATC | 2 cut(s) | 40, 211 |
| MflI | RGATCY | 1 cut(s) | 211 |
| MluCI | AATT | 2 cut(s) | 111, 329 |
| MnlI | CCTC | 3 cut(s) | 13, 66, 139 |
| MseI | TTAA | 1 cut(s) | 114 |
| MspI | CCGG | 1 cut(s) | 183 |
| MvnI | CGCG | 1 cut(s) | 367 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 15, 21, 44 |
| NdeII | GATC | 2 cut(s) | 40, 211 |
| NheI | GCTAGC | 1 cut(s) | 5 |
| NlaIII | CATG | 1 cut(s) | 28 |
| NmeAIII | GCCGAG | 1 cut(s) | 450 |
| PfeI | GAWTC | 1 cut(s) | 130 |
| PkrI | GCNGC | 3 cut(s) | 17, 49, 220 |
| PspFI | CCCAGC | 1 cut(s) | 404 |
| PspPI | GGNCC | 1 cut(s) | 402 |
| PsuI | RGATCY | 1 cut(s) | 211 |
| SaqAI | TTAA | 1 cut(s) | 114 |
| SatI | GCNGC | 3 cut(s) | 16, 48, 219 |
| Sau3AI | GATC | 2 cut(s) | 40, 211 |
| Sau96I | GGNCC | 1 cut(s) | 402 |
| SetI | ASST | 4 cut(s) | 120, 294, 301, 342 |
| Sse9I | AATT | 2 cut(s) | 111, 329 |
| SsiI | CCGC | 4 cut(s) | 15, 48, 208, 219 |
| SspMI | CTAG | 2 cut(s) | 6, 353 |
| StyI | CCWWGG | 1 cut(s) | 237 |
| TaaI | ACNGT | 3 cut(s) | 85, 92, 99 |
| TaiI | ACGT | 1 cut(s) | 120 |
| TasI | AATT | 2 cut(s) | 111, 329 |
| TauI | GCSGC | 3 cut(s) | 18, 50, 221 |
| TfiI | GAWTC | 1 cut(s) | 130 |
| Tru1I | TTAA | 1 cut(s) | 114 |
| Tru9I | TTAA | 1 cut(s) | 114 |
| TspGWI | ACGGA | 1 cut(s) | 138 |
| XapI | RAATTY | 1 cut(s) | 329 |
| XspI | CTAG | 2 cut(s) | 6, 353 |