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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 394, 543 |
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 406 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 3 cut(s) | 90, 504, 685 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AclWI | GGATC | 2 cut(s) | 201, 637 |
| AcuI | CTGAAG | 2 cut(s) | 48, 546 |
| AcyI | GRCGYC | 1 cut(s) | 660 |
| AfaI | GTAC | 3 cut(s) | 176, 381, 408 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 76, 77, 116, 151, 256, 368, 508, 610 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 5 cut(s) | 115, 347, 382, 573, 700 |
| AluBI | AGCT | 2 cut(s) | 141, 181 |
| AluI | AGCT | 2 cut(s) | 141, 181 |
| AlwI | GGATC | 2 cut(s) | 201, 637 |
| Ama87I | CYCGRG | 1 cut(s) | 151 |
| AoxI | GGCC | 5 cut(s) | 154, 246, 306, 386, 703 |
| ApeKI | GCWGC | 2 cut(s) | 138, 141 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 3 cut(s) | 231, 552, 670 |
| AspS9I | GGNCC | 5 cut(s) | 154, 247, 386, 440, 704 |
| AsuC2I | CCSGG | 5 cut(s) | 152, 153, 363, 562, 647 |
| AsuHPI | GGTGA | 3 cut(s) | 134, 602, 703 |
| AsuNHI | GCTAGC | 1 cut(s) | 181 |
| AvaI | CYCGRG | 1 cut(s) | 151 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 390, 423 |
| BanI | GGYRCC | 2 cut(s) | 379, 406 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| BbvCI | CCTCAGC | 1 cut(s) | 243 |
| BbvI | GCAGC | 2 cut(s) | 128, 150 |
| BccI | CCATC | 1 cut(s) | 59 |
| BciT130I | CCWGG | 5 cut(s) | 117, 349, 384, 575, 702 |
| BcnI | CCSGG | 5 cut(s) | 152, 153, 363, 562, 647 |
| BfaI | CTAG | 3 cut(s) | 182, 302, 513 |
| BfmI | CTRYAG | 1 cut(s) | 725 |
| BfoI | RGCGCY | 1 cut(s) | 671 |
| BfuAI | ACCTGC | 2 cut(s) | 394, 543 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 4 cut(s) | 139, 142, 145, 717 |
| BlsI | GCNGC | 4 cut(s) | 140, 143, 146, 718 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmeT110I | CYCGRG | 1 cut(s) | 151 |
| BmgT120I | GGNCC | 5 cut(s) | 154, 247, 386, 440, 704 |
| BmiI | GGNNCC | 4 cut(s) | 381, 408, 441, 706 |
| BmsI | GCATC | 1 cut(s) | 583 |
| BmtI | GCTAGC | 1 cut(s) | 185 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| BpmI | CTGGAG | 1 cut(s) | 650 |
| Bpu10I | CCTNAGC | 1 cut(s) | 243 |
| BpuMI | CCSGG | 5 cut(s) | 152, 153, 363, 562, 647 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaHI | GRCGYC | 1 cut(s) | 660 |
| BsaJI | CCNNGG | 6 cut(s) | 115, 150, 151, 348, 383, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 76, 77, 116, 151, 256, 368, 508, 610 |
| Bse118I | RCCGGY | 2 cut(s) | 388, 739 |
| Bse1I | ACTGG | 2 cut(s) | 192, 633 |
| BseBI | CCWGG | 5 cut(s) | 117, 349, 384, 575, 702 |
| BseDI | CCNNGG | 6 cut(s) | 115, 150, 151, 348, 383, 545 |
| BseGI | GGATG | 3 cut(s) | 43, 51, 570 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 76, 77, 116, 151, 256, 368, 508, 610 |
| BseMII | CTCAG | 3 cut(s) | 234, 414, 689 |
| BseNI | ACTGG | 2 cut(s) | 192, 633 |
| BseXI | GCAGC | 2 cut(s) | 128, 150 |
| BseYI | CCCAGC | 2 cut(s) | 70, 664 |
| BsgI | GTGCAG | 1 cut(s) | 416 |
| Bsh1285I | CGRYCG | 1 cut(s) | 316 |
| BshFI | GGCC | 5 cut(s) | 156, 248, 308, 388, 705 |
| BshNI | GGYRCC | 2 cut(s) | 379, 406 |
| BsiEI | CGRYCG | 1 cut(s) | 316 |
| BsiHKCI | CYCGRG | 1 cut(s) | 151 |
| BsiSI | CCGG | 6 cut(s) | 152, 363, 389, 562, 646, 740 |
| BslFI | GGGAC | 3 cut(s) | 453, 544, 647 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 76, 77, 116, 151, 256, 368, 508, 610 |
| BsmFI | GGGAC | 3 cut(s) | 453, 544, 647 |
| BsmI | GAATGC | 1 cut(s) | 624 |
| BsnI | GGCC | 5 cut(s) | 156, 248, 308, 388, 705 |
| BsoBI | CYCGRG | 1 cut(s) | 151 |
| Bsp143I | GATC | 3 cut(s) | 160, 193, 642 |
| BspANI | GGCC | 5 cut(s) | 156, 248, 308, 388, 705 |
| BspCNI | CTCAG | 3 cut(s) | 235, 415, 688 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 4 cut(s) | 381, 408, 441, 706 |
| BspMAI | CTGCAG | 1 cut(s) | 729 |
| BspMI | ACCTGC | 2 cut(s) | 394, 543 |
| BspOI | GCTAGC | 1 cut(s) | 185 |
| BspPI | GGATC | 2 cut(s) | 201, 637 |
| BspQI | GCTCTTC | 2 cut(s) | 24, 692 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 406 |
| BsrBI | CCGCTC | 3 cut(s) | 90, 504, 685 |
| BsrFI | RCCGGY | 2 cut(s) | 388, 739 |
| BsrI | ACTGG | 2 cut(s) | 192, 633 |
| BssAI | RCCGGY | 2 cut(s) | 388, 739 |
| BssECI | CCNNGG | 6 cut(s) | 115, 150, 151, 348, 383, 545 |
| BssMI | GATC | 3 cut(s) | 160, 193, 642 |
| BssNI | GRCGYC | 1 cut(s) | 660 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 5 cut(s) | 117, 349, 384, 575, 702 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 692 |
| BstACI | GRCGYC | 1 cut(s) | 660 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 183, 332, 729 |
| BstDEI | CTNAG | 3 cut(s) | 243, 423, 675 |
| BstF5I | GGATG | 3 cut(s) | 43, 51, 570 |
| BstH2I | RGCGCY | 1 cut(s) | 671 |
| BstHHI | GCGC | 3 cut(s) | 231, 552, 670 |
| BstKTI | GATC | 3 cut(s) | 163, 196, 645 |
| BstMBI | GATC | 3 cut(s) | 160, 193, 642 |
| BstMCI | CGRYCG | 1 cut(s) | 316 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 87, 144, 153, 394 |
| BstNI | CCWGG | 5 cut(s) | 117, 349, 384, 575, 702 |
| BstSFI | CTRYAG | 1 cut(s) | 725 |
| BstV1I | GCAGC | 2 cut(s) | 128, 150 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 5 cut(s) | 156, 248, 308, 388, 705 |
| BtsCI | GGATG | 3 cut(s) | 43, 51, 570 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 2 cut(s) | 394, 543 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 183, 332, 729 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 3 cut(s) | 231, 552, 670 |
| Cfr10I | RCCGGY | 2 cut(s) | 388, 739 |
| Cfr13I | GGNCC | 5 cut(s) | 154, 247, 386, 440, 704 |
| Cfr9I | CCCGGG | 1 cut(s) | 151 |
| CseI | GACGC | 3 cut(s) | 307, 409, 649 |
| Csp6I | GTAC | 3 cut(s) | 175, 380, 407 |
| CviAII | CATG | 1 cut(s) | 271 |
| CviQI | GTAC | 3 cut(s) | 175, 380, 407 |
| DdeI | CTNAG | 3 cut(s) | 243, 423, 675 |
| DpnI | GATC | 3 cut(s) | 162, 195, 644 |
| DpnII | GATC | 3 cut(s) | 160, 193, 642 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 692 |
| EarI | CTCTTC | 2 cut(s) | 24, 692 |
| EciI | GGCGGA | 3 cut(s) | 206, 525, 665 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 546 |
| Eco88I | CYCGRG | 1 cut(s) | 151 |
| EcoRII | CCWGG | 5 cut(s) | 115, 347, 382, 573, 700 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| FaeI | CATG | 1 cut(s) | 274 |
| FaiI | YATR | 5 cut(s) | 173, 201, 272, 452, 745 |
| FaqI | GGGAC | 3 cut(s) | 453, 544, 647 |
| FatI | CATG | 1 cut(s) | 270 |
| FauI | CCCGC | 5 cut(s) | 8, 55, 114, 498, 558 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 4 cut(s) | 139, 142, 145, 717 |
| FokI | GGATG | 3 cut(s) | 30, 38, 557 |
| Fsp4HI | GCNGC | 4 cut(s) | 139, 142, 145, 717 |
| FspBI | CTAG | 3 cut(s) | 182, 302, 513 |
| GlaI | GCGC | 3 cut(s) | 230, 551, 669 |
| GluI | GCNGC | 4 cut(s) | 139, 142, 145, 717 |
| GsaI | CCCAGC | 2 cut(s) | 74, 668 |
| GsuI | CTGGAG | 1 cut(s) | 650 |
| HaeII | RGCGCY | 1 cut(s) | 671 |
| HaeIII | GGCC | 5 cut(s) | 156, 248, 308, 388, 705 |
| HapII | CCGG | 6 cut(s) | 152, 363, 389, 562, 646, 740 |
| HgaI | GACGC | 3 cut(s) | 307, 409, 649 |
| HhaI | GCGC | 3 cut(s) | 231, 552, 670 |
| Hin1I | GRCGYC | 1 cut(s) | 660 |
| Hin1II | CATG | 1 cut(s) | 274 |
| Hin6I | GCGC | 3 cut(s) | 229, 550, 668 |
| HinP1I | GCGC | 3 cut(s) | 229, 550, 668 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 4 cut(s) | 482, 581, 653, 679 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 6 cut(s) | 152, 363, 389, 562, 646, 740 |
| HphI | GGTGA | 3 cut(s) | 134, 602, 703 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 4 cut(s) | 67, 508, 678, 737 |
| Hpy188III | TCNNGA | 2 cut(s) | 271, 696 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy99I | CGWCG | 1 cut(s) | 590 |
| HpyAV | CCTTC | 5 cut(s) | 217, 429, 466, 521, 718 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 3 cut(s) | 217, 277, 340 |
| HpyCH4V | TGCA | 4 cut(s) | 397, 596, 622, 727 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 87, 144, 153, 394 |
| HpyF3I | CTNAG | 3 cut(s) | 243, 423, 675 |
| HpySE526I | ACGT | 3 cut(s) | 217, 277, 340 |
| Hsp92I | GRCGYC | 1 cut(s) | 660 |
| Hsp92II | CATG | 1 cut(s) | 274 |
| HspAI | GCGC | 3 cut(s) | 229, 550, 668 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 160, 193, 642 |
| LguI | GCTCTTC | 2 cut(s) | 24, 692 |
| LmnI | GCTCC | 2 cut(s) | 226, 631 |
| Lsp1109I | GCAGC | 2 cut(s) | 128, 150 |
| LweI | GCATC | 1 cut(s) | 583 |
| MaeI | CTAG | 3 cut(s) | 182, 302, 513 |
| MaeII | ACGT | 3 cut(s) | 217, 277, 340 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 3 cut(s) | 162, 195, 644 |
| MbiI | CCGCTC | 3 cut(s) | 90, 504, 685 |
| MboI | GATC | 3 cut(s) | 160, 193, 642 |
| MboII | GAAGA | 4 cut(s) | 41, 188, 307, 679 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MlyI | GAGTC | 1 cut(s) | 662 |
| MmeI | TCCRAC | 2 cut(s) | 90, 591 |
| MnlI | CCTC | 9 cut(s) | 73, 118, 238, 277, 298, 469, 502, 513, 630 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 141 |
| MspI | CCGG | 6 cut(s) | 152, 363, 389, 562, 646, 740 |
| Mva1269I | GAATGC | 1 cut(s) | 624 |
| MvaI | CCWGG | 5 cut(s) | 117, 349, 384, 575, 702 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 87, 144, 153, 394 |
| NciI | CCSGG | 5 cut(s) | 152, 153, 363, 562, 647 |
| NdeII | GATC | 3 cut(s) | 160, 193, 642 |
| NheI | GCTAGC | 1 cut(s) | 181 |
| NlaIII | CATG | 1 cut(s) | 274 |
| NlaIV | GGNNCC | 4 cut(s) | 381, 408, 441, 706 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 2 cut(s) | 24, 692 |
| PctI | GAATGC | 1 cut(s) | 624 |
| PfeI | GAWTC | 3 cut(s) | 482, 581, 679 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 4 cut(s) | 140, 143, 146, 718 |
| PleI | GAGTC | 1 cut(s) | 661 |
| PpsI | GAGTC | 1 cut(s) | 661 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| Psp6I | CCWGG | 5 cut(s) | 115, 347, 382, 573, 700 |
| PspFI | CCCAGC | 2 cut(s) | 70, 664 |
| PspGI | CCWGG | 5 cut(s) | 115, 347, 382, 573, 700 |
| PspN4I | GGNNCC | 4 cut(s) | 381, 408, 441, 706 |
| PspPI | GGNCC | 5 cut(s) | 154, 247, 386, 440, 704 |
| PstI | CTGCAG | 1 cut(s) | 729 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuII | CAGCTG | 1 cut(s) | 141 |
| RsaI | GTAC | 3 cut(s) | 176, 381, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 380, 407 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 2 cut(s) | 24, 692 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 4 cut(s) | 139, 142, 145, 717 |
| Sau3AI | GATC | 3 cut(s) | 160, 193, 642 |
| Sau96I | GGNCC | 5 cut(s) | 154, 247, 386, 440, 704 |
| SbfI | CCTGCAGG | 1 cut(s) | 729 |
| SchI | GAGTC | 1 cut(s) | 662 |
| SdaI | CCTGCAGG | 1 cut(s) | 729 |
| SfaNI | GCATC | 1 cut(s) | 583 |
| SfcI | CTRYAG | 1 cut(s) | 725 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SmaI | CCCGGG | 1 cut(s) | 153 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 729 |
| SspI | AATATT | 1 cut(s) | 375 |
| SspMI | CTAG | 3 cut(s) | 182, 302, 513 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 3 cut(s) | 220, 280, 343 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TauI | GCSGC | 2 cut(s) | 147, 719 |
| TfiI | GAWTC | 3 cut(s) | 482, 581, 679 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 2 cut(s) | 138, 141 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspGWI | ACGGA | 1 cut(s) | 574 |
| TspMI | CCCGGG | 1 cut(s) | 151 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XmaI | CCCGGG | 1 cut(s) | 151 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XspI | CTAG | 3 cut(s) | 182, 302, 513 |