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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 94 |
| AccII | CGCG | 1 cut(s) | 191 |
| AciI | CCGC | 4 cut(s) | 76, 94, 140, 163 |
| AclI | AACGTT | 1 cut(s) | 8 |
| AclWI | GGATC | 1 cut(s) | 140 |
| AcoI | YGGCCR | 1 cut(s) | 118 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 174 |
| AgsI | TTSAA | 1 cut(s) | 27 |
| AjnI | CCWGG | 2 cut(s) | 56, 123 |
| AluBI | AGCT | 1 cut(s) | 153 |
| AluI | AGCT | 1 cut(s) | 153 |
| Alw21I | GWGCWC | 1 cut(s) | 21 |
| AlwI | GGATC | 1 cut(s) | 140 |
| AoxI | GGCC | 3 cut(s) | 98, 118, 218 |
| ApeKI | GCWGC | 1 cut(s) | 110 |
| AspLEI | GCGC | 2 cut(s) | 191, 203 |
| AspS9I | GGNCC | 2 cut(s) | 99, 165 |
| AsuC2I | CCSGG | 1 cut(s) | 107 |
| AvaII | GGWCC | 1 cut(s) | 165 |
| BanII | GRGCYC | 1 cut(s) | 64 |
| Bbv12I | GWGCWC | 1 cut(s) | 21 |
| BbvI | GCAGC | 1 cut(s) | 122 |
| BciT130I | CCWGG | 2 cut(s) | 58, 125 |
| BcnI | CCSGG | 1 cut(s) | 107 |
| BfaI | CTAG | 1 cut(s) | 44 |
| BisI | GCNGC | 2 cut(s) | 111, 140 |
| BlsI | GCNGC | 2 cut(s) | 112, 141 |
| Bme1390I | CCNGG | 3 cut(s) | 58, 107, 125 |
| Bme18I | GGWCC | 1 cut(s) | 165 |
| BmgT120I | GGNCC | 2 cut(s) | 99, 165 |
| BmrFI | CCNGG | 3 cut(s) | 58, 107, 125 |
| BpuMI | CCSGG | 1 cut(s) | 107 |
| BsaJI | CCNNGG | 2 cut(s) | 56, 57 |
| BsaWI | WCCGGW | 2 cut(s) | 33, 88 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 174 |
| BseBI | CCWGG | 2 cut(s) | 58, 125 |
| BseDI | CCNNGG | 2 cut(s) | 56, 57 |
| BseGI | GGATG | 1 cut(s) | 64 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 174 |
| BseXI | GCAGC | 1 cut(s) | 122 |
| Bsh1236I | CGCG | 1 cut(s) | 191 |
| Bsh1285I | CGRYCG | 1 cut(s) | 106 |
| BshFI | GGCC | 3 cut(s) | 100, 120, 220 |
| BsiEI | CGRYCG | 1 cut(s) | 106 |
| BsiHKAI | GWGCWC | 1 cut(s) | 21 |
| BsiSI | CCGG | 3 cut(s) | 34, 89, 106 |
| BslFI | GGGAC | 1 cut(s) | 58 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 174 |
| BsmFI | GGGAC | 1 cut(s) | 58 |
| BsnI | GGCC | 3 cut(s) | 100, 120, 220 |
| Bsp1286I | GDGCHC | 2 cut(s) | 21, 64 |
| Bsp143I | GATC | 1 cut(s) | 132 |
| BspACI | CCGC | 4 cut(s) | 76, 94, 140, 163 |
| BspANI | GGCC | 3 cut(s) | 100, 120, 220 |
| BspFNI | CGCG | 1 cut(s) | 191 |
| BspPI | GGATC | 1 cut(s) | 140 |
| BsrBI | CCGCTC | 1 cut(s) | 94 |
| BssECI | CCNNGG | 2 cut(s) | 56, 57 |
| BssMI | GATC | 1 cut(s) | 132 |
| Bst2UI | CCWGG | 2 cut(s) | 58, 125 |
| BstC8I | GCNNGC | 1 cut(s) | 155 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 172 |
| BstF5I | GGATG | 1 cut(s) | 64 |
| BstFNI | CGCG | 1 cut(s) | 191 |
| BstHHI | GCGC | 2 cut(s) | 191, 203 |
| BstKTI | GATC | 1 cut(s) | 135 |
| BstMBI | GATC | 1 cut(s) | 132 |
| BstMCI | CGRYCG | 1 cut(s) | 106 |
| BstNI | CCWGG | 2 cut(s) | 58, 125 |
| BstSCI | CCNGG | 3 cut(s) | 56, 105, 123 |
| BstUI | CGCG | 1 cut(s) | 191 |
| BstV1I | GCAGC | 1 cut(s) | 122 |
| BstX2I | RGATCY | 1 cut(s) | 132 |
| BstYI | RGATCY | 1 cut(s) | 132 |
| BsuRI | GGCC | 3 cut(s) | 100, 120, 220 |
| BtsCI | GGATG | 1 cut(s) | 64 |
| Cac8I | GCNNGC | 1 cut(s) | 155 |
| CfoI | GCGC | 2 cut(s) | 191, 203 |
| Cfr13I | GGNCC | 2 cut(s) | 99, 165 |
| CseI | GACGC | 1 cut(s) | 36 |
| CsiI | ACCWGGT | 1 cut(s) | 123 |
| CviJI | RGCY | 5 cut(s) | 62, 100, 120, 153, 220 |
| CviKI_1 | RGCY | 5 cut(s) | 62, 100, 120, 153, 220 |
| DpnI | GATC | 1 cut(s) | 134 |
| DpnII | GATC | 1 cut(s) | 132 |
| EaeI | YGGCCR | 1 cut(s) | 118 |
| EciI | GGCGGA | 1 cut(s) | 178 |
| Eco147I | AGGCCT | 1 cut(s) | 220 |
| Eco24I | GRGCYC | 1 cut(s) | 64 |
| Eco47I | GGWCC | 1 cut(s) | 165 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 172 |
| EcoRII | CCWGG | 2 cut(s) | 56, 123 |
| EcoT38I | GRGCYC | 1 cut(s) | 64 |
| FaiI | YATR | 2 cut(s) | 145, 149 |
| FaqI | GGGAC | 1 cut(s) | 58 |
| FauI | CCCGC | 1 cut(s) | 83 |
| Fnu4HI | GCNGC | 2 cut(s) | 111, 140 |
| FokI | GGATG | 1 cut(s) | 51 |
| FriOI | GRGCYC | 1 cut(s) | 64 |
| Fsp4HI | GCNGC | 2 cut(s) | 111, 140 |
| FspBI | CTAG | 1 cut(s) | 44 |
| GlaI | GCGC | 2 cut(s) | 190, 202 |
| GluI | GCNGC | 2 cut(s) | 111, 140 |
| HaeIII | GGCC | 3 cut(s) | 100, 120, 220 |
| HapII | CCGG | 3 cut(s) | 34, 89, 106 |
| HgaI | GACGC | 1 cut(s) | 36 |
| HhaI | GCGC | 2 cut(s) | 191, 203 |
| Hin6I | GCGC | 2 cut(s) | 189, 201 |
| HinP1I | GCGC | 2 cut(s) | 189, 201 |
| HincII | GTYRAC | 3 cut(s) | 6, 12, 39 |
| HindII | GTYRAC | 3 cut(s) | 6, 12, 39 |
| HpaI | GTTAAC | 2 cut(s) | 6, 12 |
| HpaII | CCGG | 3 cut(s) | 34, 89, 106 |
| Hpy166II | GTNNAC | 3 cut(s) | 6, 12, 39 |
| Hpy188III | TCNNGA | 1 cut(s) | 130 |
| Hpy8I | GTNNAC | 3 cut(s) | 6, 12, 39 |
| Hpy99I | CGWCG | 1 cut(s) | 52 |
| HpyCH4IV | ACGT | 1 cut(s) | 8 |
| HpySE526I | ACGT | 1 cut(s) | 8 |
| HspAI | GCGC | 2 cut(s) | 189, 201 |
| KspAI | GTTAAC | 2 cut(s) | 6, 12 |
| Kzo9I | GATC | 1 cut(s) | 132 |
| LmnI | GCTCC | 2 cut(s) | 91, 186 |
| Lsp1109I | GCAGC | 1 cut(s) | 122 |
| MabI | ACCWGGT | 1 cut(s) | 123 |
| MaeI | CTAG | 1 cut(s) | 44 |
| MaeII | ACGT | 1 cut(s) | 8 |
| MalI | GATC | 1 cut(s) | 134 |
| MbiI | CCGCTC | 1 cut(s) | 94 |
| MboI | GATC | 1 cut(s) | 132 |
| MflI | RGATCY | 1 cut(s) | 132 |
| MhlI | GDGCHC | 2 cut(s) | 21, 64 |
| MnlI | CCTC | 3 cut(s) | 90, 178, 210 |
| MseI | TTAA | 2 cut(s) | 5, 11 |
| MspI | CCGG | 3 cut(s) | 34, 89, 106 |
| MspR9I | CCNGG | 3 cut(s) | 58, 107, 125 |
| MvaI | CCWGG | 2 cut(s) | 58, 125 |
| MvnI | CGCG | 1 cut(s) | 191 |
| NciI | CCSGG | 1 cut(s) | 107 |
| NdeII | GATC | 1 cut(s) | 132 |
| PasI | CCCWGGG | 1 cut(s) | 57 |
| PceI | AGGCCT | 1 cut(s) | 220 |
| PkrI | GCNGC | 2 cut(s) | 112, 141 |
| Psp1406I | AACGTT | 1 cut(s) | 8 |
| Psp6I | CCWGG | 2 cut(s) | 56, 123 |
| PspGI | CCWGG | 2 cut(s) | 56, 123 |
| PspPI | GGNCC | 2 cut(s) | 99, 165 |
| PsuI | RGATCY | 1 cut(s) | 132 |
| SaqAI | TTAA | 2 cut(s) | 5, 11 |
| SatI | GCNGC | 2 cut(s) | 111, 140 |
| Sau3AI | GATC | 1 cut(s) | 132 |
| Sau96I | GGNCC | 2 cut(s) | 99, 165 |
| ScrFI | CCNGG | 3 cut(s) | 58, 107, 125 |
| SduI | GDGCHC | 2 cut(s) | 21, 64 |
| SetI | ASST | 5 cut(s) | 11, 129, 155, 170, 215 |
| SexAI | ACCWGGT | 1 cut(s) | 123 |
| SinI | GGWCC | 1 cut(s) | 165 |
| SseBI | AGGCCT | 1 cut(s) | 220 |
| SsiI | CCGC | 4 cut(s) | 76, 94, 140, 163 |
| SspMI | CTAG | 1 cut(s) | 44 |
| StuI | AGGCCT | 1 cut(s) | 220 |
| StyD4I | CCNGG | 3 cut(s) | 56, 105, 123 |
| TaiI | ACGT | 1 cut(s) | 11 |
| TaqI | TCGA | 2 cut(s) | 50, 215 |
| TauI | GCSGC | 1 cut(s) | 142 |
| Tru1I | TTAA | 2 cut(s) | 5, 11 |
| Tru9I | TTAA | 2 cut(s) | 5, 11 |
| TseI | GCWGC | 1 cut(s) | 110 |
| VpaK11BI | GGWCC | 1 cut(s) | 165 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 172 |
| XspI | CTAG | 1 cut(s) | 44 |