No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 310 |
| Acc36I | ACCTGC | 1 cut(s) | 310 |
| AccII | CGCG | 1 cut(s) | 108 |
| AciI | CCGC | 9 cut(s) | 15, 48, 92, 106, 185, 231, 262, 503, 533 |
| AcuI | CTGAAG | 1 cut(s) | 374 |
| AfaI | GTAC | 2 cut(s) | 239, 462 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 139, 319, 431 |
| AgsI | TTSAA | 4 cut(s) | 118, 334, 347, 469 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 428 |
| AjnI | CCWGG | 1 cut(s) | 214 |
| AluBI | AGCT | 1 cut(s) | 481 |
| AluI | AGCT | 1 cut(s) | 481 |
| Alw26I | GTCTC | 2 cut(s) | 68, 169 |
| AoxI | GGCC | 2 cut(s) | 303, 309 |
| AspLEI | GCGC | 3 cut(s) | 110, 364, 437 |
| AspS9I | GGNCC | 3 cut(s) | 223, 310, 494 |
| AsuHPI | GGTGA | 1 cut(s) | 159 |
| AvaII | GGWCC | 2 cut(s) | 223, 494 |
| BciT130I | CCWGG | 1 cut(s) | 216 |
| BcoDI | GTCTC | 2 cut(s) | 68, 169 |
| BfaI | CTAG | 3 cut(s) | 135, 198, 541 |
| BfoI | RGCGCY | 1 cut(s) | 365 |
| BfuAI | ACCTGC | 1 cut(s) | 310 |
| BglII | AGATCT | 1 cut(s) | 256 |
| BisI | GCNGC | 1 cut(s) | 231 |
| BlsI | GCNGC | 1 cut(s) | 232 |
| Bme1390I | CCNGG | 1 cut(s) | 216 |
| Bme18I | GGWCC | 2 cut(s) | 223, 494 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 428 |
| BmgT120I | GGNCC | 3 cut(s) | 223, 310, 494 |
| BmiI | GGNNCC | 3 cut(s) | 33, 144, 495 |
| BmrFI | CCNGG | 1 cut(s) | 216 |
| BmsI | GCATC | 1 cut(s) | 166 |
| BpmI | CTGGAG | 1 cut(s) | 132 |
| Bpu10I | CCTNAGC | 1 cut(s) | 477 |
| BsaI | GGTCTC | 1 cut(s) | 169 |
| BsaJI | CCNNGG | 1 cut(s) | 306 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 139, 319, 431 |
| Bse118I | RCCGGY | 1 cut(s) | 34 |
| Bse1I | ACTGG | 1 cut(s) | 255 |
| BseBI | CCWGG | 1 cut(s) | 216 |
| BseDI | CCNNGG | 1 cut(s) | 306 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 139, 319, 431 |
| BseMII | CTCAG | 2 cut(s) | 63, 468 |
| BseNI | ACTGG | 1 cut(s) | 255 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseYI | CCCAGC | 2 cut(s) | 88, 442 |
| Bsh1236I | CGCG | 1 cut(s) | 108 |
| BshFI | GGCC | 2 cut(s) | 305, 311 |
| BsiSI | CCGG | 1 cut(s) | 35 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 139, 319, 431 |
| BsmAI | GTCTC | 2 cut(s) | 68, 169 |
| BsmI | GAATGC | 1 cut(s) | 437 |
| BsnI | GGCC | 2 cut(s) | 305, 311 |
| Bso31I | GGTCTC | 1 cut(s) | 169 |
| Bsp143I | GATC | 2 cut(s) | 40, 256 |
| BspACI | CCGC | 9 cut(s) | 15, 48, 92, 106, 185, 231, 262, 503, 533 |
| BspANI | GGCC | 2 cut(s) | 305, 311 |
| BspCNI | CTCAG | 2 cut(s) | 64, 469 |
| BspFNI | CGCG | 1 cut(s) | 108 |
| BspLI | GGNNCC | 3 cut(s) | 33, 144, 495 |
| BspMI | ACCTGC | 1 cut(s) | 310 |
| BspTNI | GGTCTC | 1 cut(s) | 169 |
| BsrFI | RCCGGY | 1 cut(s) | 34 |
| BsrI | ACTGG | 1 cut(s) | 255 |
| BssAI | RCCGGY | 1 cut(s) | 34 |
| BssECI | CCNNGG | 1 cut(s) | 306 |
| BssMI | GATC | 2 cut(s) | 40, 256 |
| BssT1I | CCWWGG | 1 cut(s) | 306 |
| Bst2UI | CCWGG | 1 cut(s) | 216 |
| Bst4CI | ACNGT | 1 cut(s) | 465 |
| Bst6I | CTCTTC | 1 cut(s) | 350 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 395 |
| BstDEI | CTNAG | 2 cut(s) | 72, 477 |
| BstFNI | CGCG | 1 cut(s) | 108 |
| BstH2I | RGCGCY | 1 cut(s) | 365 |
| BstHHI | GCGC | 3 cut(s) | 110, 364, 437 |
| BstKTI | GATC | 2 cut(s) | 43, 259 |
| BstMAI | GTCTC | 2 cut(s) | 68, 169 |
| BstMBI | GATC | 2 cut(s) | 40, 256 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 368, 443 |
| BstNI | CCWGG | 1 cut(s) | 216 |
| BstSCI | CCNGG | 1 cut(s) | 214 |
| BstUI | CGCG | 1 cut(s) | 108 |
| BstX2I | RGATCY | 1 cut(s) | 256 |
| BstYI | RGATCY | 1 cut(s) | 256 |
| BsuRI | GGCC | 2 cut(s) | 305, 311 |
| BtsIMutI | CAGTG | 1 cut(s) | 248 |
| BveI | ACCTGC | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 395 |
| CfoI | GCGC | 3 cut(s) | 110, 364, 437 |
| Cfr10I | RCCGGY | 1 cut(s) | 34 |
| Cfr13I | GGNCC | 3 cut(s) | 223, 310, 494 |
| CsiI | ACCWGGT | 1 cut(s) | 214 |
| Csp6I | GTAC | 2 cut(s) | 238, 461 |
| CviAII | CATG | 3 cut(s) | 139, 235, 409 |
| CviJI | RGCY | 5 cut(s) | 34, 145, 305, 311, 481 |
| CviKI_1 | RGCY | 5 cut(s) | 34, 145, 305, 311, 481 |
| CviQI | GTAC | 2 cut(s) | 238, 461 |
| DdeI | CTNAG | 2 cut(s) | 72, 477 |
| DpnI | GATC | 2 cut(s) | 42, 258 |
| DpnII | GATC | 2 cut(s) | 40, 256 |
| DriI | GACNNNNNGTC | 1 cut(s) | 428 |
| Eam1104I | CTCTTC | 1 cut(s) | 350 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 428 |
| EarI | CTCTTC | 1 cut(s) | 350 |
| EciI | GGCGGA | 2 cut(s) | 37, 492 |
| Eco130I | CCWWGG | 1 cut(s) | 306 |
| Eco31I | GGTCTC | 1 cut(s) | 169 |
| Eco47I | GGWCC | 2 cut(s) | 223, 494 |
| Eco57I | CTGAAG | 1 cut(s) | 374 |
| EcoO109I | RGGNCCY | 1 cut(s) | 223 |
| EcoRII | CCWGG | 1 cut(s) | 214 |
| EcoT14I | CCWWGG | 1 cut(s) | 306 |
| ErhI | CCWWGG | 1 cut(s) | 306 |
| FaeI | CATG | 3 cut(s) | 142, 238, 412 |
| FaiI | YATR | 4 cut(s) | 140, 236, 294, 410 |
| FatI | CATG | 3 cut(s) | 138, 234, 408 |
| FauI | CCCGC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 1 cut(s) | 231 |
| Fsp4HI | GCNGC | 1 cut(s) | 231 |
| FspBI | CTAG | 3 cut(s) | 135, 198, 541 |
| GlaI | GCGC | 3 cut(s) | 109, 363, 436 |
| GluI | GCNGC | 1 cut(s) | 231 |
| GsaI | CCCAGC | 2 cut(s) | 92, 446 |
| GsuI | CTGGAG | 1 cut(s) | 132 |
| HaeII | RGCGCY | 1 cut(s) | 365 |
| HaeIII | GGCC | 2 cut(s) | 305, 311 |
| HapII | CCGG | 1 cut(s) | 35 |
| HhaI | GCGC | 3 cut(s) | 110, 364, 437 |
| Hin1II | CATG | 3 cut(s) | 142, 238, 412 |
| Hin6I | GCGC | 3 cut(s) | 108, 362, 435 |
| HinP1I | GCGC | 3 cut(s) | 108, 362, 435 |
| HincII | GTYRAC | 2 cut(s) | 85, 97 |
| HindII | GTYRAC | 2 cut(s) | 85, 97 |
| HinfI | GANTC | 1 cut(s) | 536 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 1 cut(s) | 35 |
| HphI | GGTGA | 1 cut(s) | 159 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 97, 212, 497 |
| Hpy188III | TCNNGA | 1 cut(s) | 221 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 97, 212, 497 |
| Hpy99I | CGWCG | 1 cut(s) | 434 |
| HpyAV | CCTTC | 1 cut(s) | 521 |
| HpyCH4III | ACNGT | 1 cut(s) | 465 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 429 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 368, 443 |
| HpyF3I | CTNAG | 2 cut(s) | 72, 477 |
| HpySE526I | ACGT | 2 cut(s) | 81, 429 |
| Hsp92II | CATG | 3 cut(s) | 142, 238, 412 |
| HspAI | GCGC | 3 cut(s) | 108, 362, 435 |
| KroI | GCCGGC | 1 cut(s) | 34 |
| KroNI | GCCGGC | 1 cut(s) | 36 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 40, 256 |
| LmnI | GCTCC | 3 cut(s) | 31, 142, 523 |
| LweI | GCATC | 1 cut(s) | 166 |
| MabI | ACCWGGT | 1 cut(s) | 214 |
| MaeI | CTAG | 3 cut(s) | 135, 198, 541 |
| MaeII | ACGT | 2 cut(s) | 81, 429 |
| MaeIII | GTNAC | 1 cut(s) | 325 |
| MalI | GATC | 2 cut(s) | 42, 258 |
| MboI | GATC | 2 cut(s) | 40, 256 |
| MboII | GAAGA | 2 cut(s) | 359, 367 |
| MflI | RGATCY | 1 cut(s) | 256 |
| MnlI | CCTC | 6 cut(s) | 22, 156, 236, 351, 405, 510 |
| MroNI | GCCGGC | 1 cut(s) | 34 |
| MseI | TTAA | 2 cut(s) | 84, 102 |
| MspA1I | CMGCKG | 2 cut(s) | 92, 185 |
| MspI | CCGG | 1 cut(s) | 35 |
| MspR9I | CCNGG | 1 cut(s) | 216 |
| Mva1269I | GAATGC | 1 cut(s) | 437 |
| MvaI | CCWGG | 1 cut(s) | 216 |
| MvnI | CGCG | 1 cut(s) | 108 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 368, 443 |
| NaeI | GCCGGC | 1 cut(s) | 36 |
| NdeII | GATC | 2 cut(s) | 40, 256 |
| NgoMIV | GCCGGC | 1 cut(s) | 34 |
| NlaIII | CATG | 3 cut(s) | 142, 238, 412 |
| NlaIV | GGNNCC | 3 cut(s) | 33, 144, 495 |
| NmuCI | GTSAC | 1 cut(s) | 325 |
| PaqCI | CACCTGC | 1 cut(s) | 310 |
| PctI | GAATGC | 1 cut(s) | 437 |
| PdiI | GCCGGC | 1 cut(s) | 36 |
| PfeI | GAWTC | 1 cut(s) | 536 |
| PkrI | GCNGC | 1 cut(s) | 232 |
| PpuMI | RGGWCCY | 1 cut(s) | 223 |
| Psp5II | RGGWCCY | 1 cut(s) | 223 |
| Psp6I | CCWGG | 1 cut(s) | 214 |
| PspFI | CCCAGC | 2 cut(s) | 88, 442 |
| PspGI | CCWGG | 1 cut(s) | 214 |
| PspN4I | GGNNCC | 3 cut(s) | 33, 144, 495 |
| PspPI | GGNCC | 3 cut(s) | 223, 310, 494 |
| PspPPI | RGGWCCY | 1 cut(s) | 223 |
| PsuI | RGATCY | 1 cut(s) | 256 |
| RsaI | GTAC | 2 cut(s) | 239, 462 |
| RsaNI | GTAC | 2 cut(s) | 238, 461 |
| SaqAI | TTAA | 2 cut(s) | 84, 102 |
| SatI | GCNGC | 1 cut(s) | 231 |
| Sau3AI | GATC | 2 cut(s) | 40, 256 |
| Sau96I | GGNCC | 3 cut(s) | 223, 310, 494 |
| ScrFI | CCNGG | 1 cut(s) | 216 |
| SetI | ASST | 9 cut(s) | 84, 199, 217, 228, 324, 432, 478, 483, 502 |
| SexAI | ACCWGGT | 1 cut(s) | 214 |
| SfaNI | GCATC | 1 cut(s) | 166 |
| SinI | GGWCC | 2 cut(s) | 223, 494 |
| SsiI | CCGC | 9 cut(s) | 15, 48, 92, 106, 185, 231, 262, 503, 533 |
| SspI | AATATT | 1 cut(s) | 519 |
| SspMI | CTAG | 3 cut(s) | 135, 198, 541 |
| StyD4I | CCNGG | 1 cut(s) | 214 |
| StyI | CCWWGG | 1 cut(s) | 306 |
| TaaI | ACNGT | 1 cut(s) | 465 |
| TaiI | ACGT | 2 cut(s) | 84, 432 |
| TauI | GCSGC | 1 cut(s) | 233 |
| TfiI | GAWTC | 1 cut(s) | 536 |
| Tru1I | TTAA | 2 cut(s) | 84, 102 |
| Tru9I | TTAA | 2 cut(s) | 84, 102 |
| TscAI | CASTG | 1 cut(s) | 255 |
| TseFI | GTSAC | 1 cut(s) | 325 |
| Tsp45I | GTSAC | 1 cut(s) | 325 |
| TspRI | CASTG | 1 cut(s) | 255 |
| VpaK11BI | GGWCC | 2 cut(s) | 223, 494 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 241 |
| XspI | CTAG | 3 cut(s) | 135, 198, 541 |