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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 704 |
| Acc36I | ACCTGC | 2 cut(s) | 704, 797 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 851 |
| AccBSI | CCGCTC | 1 cut(s) | 575 |
| AccI | GTMKAC | 1 cut(s) | 599 |
| AccII | CGCG | 5 cut(s) | 60, 75, 286, 507, 962 |
| AclWI | GGATC | 4 cut(s) | 409, 442, 768, 878 |
| AcoI | YGGCCR | 2 cut(s) | 502, 547 |
| AcuI | CTGAAG | 3 cut(s) | 120, 133, 978 |
| AcyI | GRCGYC | 1 cut(s) | 300 |
| AdeI | CACNNNGTG | 1 cut(s) | 935 |
| AfaI | GTAC | 3 cut(s) | 417, 637, 649 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 13, 172, 609, 760, 787, 851 |
| AflIII | ACRYGT | 1 cut(s) | 516 |
| AgsI | TTSAA | 2 cut(s) | 208, 272 |
| AjiI | CACGTC | 1 cut(s) | 1068 |
| AjnI | CCWGG | 6 cut(s) | 374, 397, 588, 623, 662, 753 |
| AluBI | AGCT | 3 cut(s) | 95, 104, 848 |
| AluI | AGCT | 3 cut(s) | 95, 104, 848 |
| Alw26I | GTCTC | 4 cut(s) | 159, 294, 488, 888 |
| AlwI | GGATC | 4 cut(s) | 409, 442, 768, 878 |
| AoxI | GGCC | 9 cut(s) | 395, 481, 502, 547, 627, 795, 817, 920, 989 |
| ApeKI | GCWGC | 6 cut(s) | 18, 379, 382, 486, 583, 946 |
| AseI | ATTAAT | 1 cut(s) | 195 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 727 |
| AspA2I | CCTAGG | 1 cut(s) | 786 |
| AspLEI | GCGC | 8 cut(s) | 62, 77, 286, 472, 509, 588, 609, 623 |
| AspS9I | GGNCC | 4 cut(s) | 35, 627, 681, 1015 |
| AsuC2I | CCSGG | 3 cut(s) | 358, 561, 888 |
| AsuHPI | GGTGA | 5 cut(s) | 155, 375, 401, 783, 1021 |
| AvaII | GGWCC | 3 cut(s) | 35, 681, 1015 |
| AvrII | CCTAGG | 1 cut(s) | 786 |
| BbsI | GAAGAC | 1 cut(s) | 279 |
| BbvCI | CCTCAGC | 1 cut(s) | 26 |
| BbvI | GCAGC | 6 cut(s) | 5, 391, 394, 498, 595, 958 |
| BccI | CCATC | 3 cut(s) | 76, 494, 1052 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 723, 757 |
| BciT130I | CCWGG | 6 cut(s) | 376, 399, 590, 625, 664, 755 |
| BcnI | CCSGG | 3 cut(s) | 358, 561, 888 |
| BcoDI | GTCTC | 4 cut(s) | 159, 294, 488, 888 |
| BfaI | CTAG | 2 cut(s) | 787, 967 |
| BfoI | RGCGCY | 3 cut(s) | 589, 610, 624 |
| BfuAI | ACCTGC | 2 cut(s) | 704, 797 |
| BglI | GCCNNNNNGGC | 4 cut(s) | 15, 83, 394, 803 |
| BlnI | CCTAGG | 1 cut(s) | 786 |
| Bme1390I | CCNGG | 9 cut(s) | 358, 376, 399, 561, 590, 625, 664, 755, 888 |
| Bme18I | GGWCC | 3 cut(s) | 35, 681, 1015 |
| BmgBI | CACGTC | 1 cut(s) | 1068 |
| BmgT120I | GGNCC | 4 cut(s) | 35, 627, 681, 1015 |
| BmiI | GGNNCC | 3 cut(s) | 333, 682, 1016 |
| BmrFI | CCNGG | 9 cut(s) | 358, 376, 399, 561, 590, 625, 664, 755, 888 |
| BmsI | GCATC | 6 cut(s) | 39, 95, 268, 850, 958, 991 |
| BpiI | GAAGAC | 1 cut(s) | 279 |
| BpmI | CTGGAG | 1 cut(s) | 27 |
| Bpu10I | CCTNAGC | 1 cut(s) | 26 |
| BpuEI | CTTGAG | 1 cut(s) | 994 |
| BpuMI | CCSGG | 3 cut(s) | 358, 561, 888 |
| BsaHI | GRCGYC | 1 cut(s) | 300 |
| BsaI | GGTCTC | 1 cut(s) | 294 |
| BsaJI | CCNNGG | 3 cut(s) | 624, 663, 786 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 864, 894 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 13, 172, 609, 760, 787, 851 |
| Bse118I | RCCGGY | 3 cut(s) | 609, 629, 828 |
| Bse1I | ACTGG | 1 cut(s) | 433 |
| BseBI | CCWGG | 6 cut(s) | 376, 399, 590, 625, 664, 755 |
| BseDI | CCNNGG | 3 cut(s) | 624, 663, 786 |
| BseGI | GGATG | 1 cut(s) | 1044 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 13, 172, 609, 760, 787, 851 |
| BseMII | CTCAG | 2 cut(s) | 40, 757 |
| BseNI | ACTGG | 1 cut(s) | 433 |
| BseRI | GAGGAG | 5 cut(s) | 172, 179, 477, 480, 997 |
| BseXI | GCAGC | 6 cut(s) | 5, 391, 394, 498, 595, 958 |
| BseYI | CCCAGC | 4 cut(s) | 104, 280, 603, 844 |
| Bsh1236I | CGCG | 5 cut(s) | 60, 75, 286, 507, 962 |
| Bsh1285I | CGRYCG | 2 cut(s) | 557, 634 |
| BshFI | GGCC | 9 cut(s) | 397, 483, 504, 549, 629, 797, 819, 922, 991 |
| BsiEI | CGRYCG | 2 cut(s) | 557, 634 |
| BsiSI | CCGG | 6 cut(s) | 358, 561, 610, 630, 829, 887 |
| BslFI | GGGAC | 3 cut(s) | 110, 694, 1028 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 13, 172, 609, 760, 787, 851 |
| BsmAI | GTCTC | 4 cut(s) | 159, 294, 488, 888 |
| BsmBI | CGTCTC | 1 cut(s) | 888 |
| BsmFI | GGGAC | 3 cut(s) | 110, 694, 1028 |
| BsmI | GAATGC | 2 cut(s) | 203, 755 |
| BsnI | GGCC | 9 cut(s) | 397, 483, 504, 549, 629, 797, 819, 922, 991 |
| Bso31I | GGTCTC | 1 cut(s) | 294 |
| Bsp143I | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| BspANI | GGCC | 9 cut(s) | 397, 483, 504, 549, 629, 797, 819, 922, 991 |
| BspCNI | CTCAG | 2 cut(s) | 39, 758 |
| BspFNI | CGCG | 5 cut(s) | 60, 75, 286, 507, 962 |
| BspHI | TCATGA | 2 cut(s) | 511, 1070 |
| BspLI | GGNNCC | 3 cut(s) | 333, 682, 1016 |
| BspMI | ACCTGC | 2 cut(s) | 704, 797 |
| BspPI | GGATC | 4 cut(s) | 409, 442, 768, 878 |
| BspTNI | GGTCTC | 1 cut(s) | 294 |
| BsrBI | CCGCTC | 1 cut(s) | 575 |
| BsrFI | RCCGGY | 3 cut(s) | 609, 629, 828 |
| BsrI | ACTGG | 1 cut(s) | 433 |
| BssAI | RCCGGY | 3 cut(s) | 609, 629, 828 |
| BssECI | CCNNGG | 3 cut(s) | 624, 663, 786 |
| BssMI | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| BssNI | GRCGYC | 1 cut(s) | 300 |
| BssT1I | CCWWGG | 1 cut(s) | 786 |
| Bst2UI | CCWGG | 6 cut(s) | 376, 399, 590, 625, 664, 755 |
| Bst4CI | ACNGT | 2 cut(s) | 222, 1019 |
| Bst6I | CTCTTC | 1 cut(s) | 162 |
| BstACI | GRCGYC | 1 cut(s) | 300 |
| BstC8I | GCNNGC | 4 cut(s) | 573, 611, 830, 941 |
| BstDEI | CTNAG | 3 cut(s) | 26, 254, 766 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 170 |
| BstF5I | GGATG | 1 cut(s) | 1044 |
| BstFNI | CGCG | 5 cut(s) | 60, 75, 286, 507, 962 |
| BstH2I | RGCGCY | 3 cut(s) | 589, 610, 624 |
| BstHHI | GCGC | 8 cut(s) | 62, 77, 286, 472, 509, 588, 609, 623 |
| BstKTI | GATC | 7 cut(s) | 49, 151, 404, 437, 776, 886, 966 |
| BstMAI | GTCTC | 4 cut(s) | 159, 294, 488, 888 |
| BstMBI | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| BstMCI | CGRYCG | 2 cut(s) | 557, 634 |
| BstNI | CCWGG | 6 cut(s) | 376, 399, 590, 625, 664, 755 |
| BstNSI | RCATGY | 1 cut(s) | 648 |
| BstSCI | CCNGG | 9 cut(s) | 356, 374, 397, 559, 588, 623, 662, 753, 886 |
| BstUI | CGCG | 5 cut(s) | 60, 75, 286, 507, 962 |
| BstV1I | GCAGC | 6 cut(s) | 5, 391, 394, 498, 595, 958 |
| BstV2I | GAAGAC | 1 cut(s) | 279 |
| BstX2I | RGATCY | 2 cut(s) | 401, 434 |
| BstYI | RGATCY | 2 cut(s) | 401, 434 |
| BsuRI | GGCC | 9 cut(s) | 397, 483, 504, 549, 629, 797, 819, 922, 991 |
| BtrI | CACGTC | 1 cut(s) | 1068 |
| BtsCI | GGATG | 1 cut(s) | 1044 |
| BtsIMutI | CAGTG | 2 cut(s) | 340, 426 |
| BveI | ACCTGC | 2 cut(s) | 704, 797 |
| Cac8I | GCNNGC | 4 cut(s) | 573, 611, 830, 941 |
| CciI | TCATGA | 2 cut(s) | 511, 1070 |
| CfoI | GCGC | 8 cut(s) | 62, 77, 286, 472, 509, 588, 609, 623 |
| Cfr10I | RCCGGY | 3 cut(s) | 609, 629, 828 |
| Cfr13I | GGNCC | 4 cut(s) | 35, 627, 681, 1015 |
| CseI | GACGC | 1 cut(s) | 308 |
| Csp6I | GTAC | 3 cut(s) | 416, 636, 648 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 649, 684 |
| CviAII | CATG | 4 cut(s) | 413, 512, 645, 1071 |
| CviQI | GTAC | 3 cut(s) | 416, 636, 648 |
| DdeI | CTNAG | 3 cut(s) | 26, 254, 766 |
| DpnI | GATC | 7 cut(s) | 48, 150, 403, 436, 775, 885, 965 |
| DpnII | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| DraIII | CACNNNGTG | 1 cut(s) | 935 |
| EaeI | YGGCCR | 2 cut(s) | 502, 547 |
| Eam1104I | CTCTTC | 1 cut(s) | 162 |
| EarI | CTCTTC | 1 cut(s) | 162 |
| EciI | GGCGGA | 5 cut(s) | 447, 735, 766, 815, 906 |
| Eco130I | CCWWGG | 1 cut(s) | 786 |
| Eco147I | AGGCCT | 1 cut(s) | 922 |
| Eco31I | GGTCTC | 1 cut(s) | 294 |
| Eco47I | GGWCC | 3 cut(s) | 35, 681, 1015 |
| Eco57I | CTGAAG | 3 cut(s) | 120, 133, 978 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 170 |
| EcoRII | CCWGG | 6 cut(s) | 374, 397, 588, 623, 662, 753 |
| EcoT14I | CCWWGG | 1 cut(s) | 786 |
| EcoT22I | ATGCAT | 1 cut(s) | 646 |
| ErhI | CCWWGG | 1 cut(s) | 786 |
| Esp3I | CGTCTC | 1 cut(s) | 888 |
| FaeI | CATG | 4 cut(s) | 416, 515, 648, 1074 |
| FaqI | GGGAC | 3 cut(s) | 110, 694, 1028 |
| FatI | CATG | 4 cut(s) | 412, 511, 644, 1070 |
| FauI | CCCGC | 1 cut(s) | 932 |
| FblI | GTMKAC | 1 cut(s) | 599 |
| FokI | GGATG | 1 cut(s) | 1031 |
| FspBI | CTAG | 2 cut(s) | 787, 967 |
| GlaI | GCGC | 8 cut(s) | 61, 76, 285, 471, 508, 587, 608, 622 |
| GsaI | CCCAGC | 4 cut(s) | 108, 284, 607, 848 |
| GsuI | CTGGAG | 1 cut(s) | 27 |
| HaeII | RGCGCY | 3 cut(s) | 589, 610, 624 |
| HaeIII | GGCC | 9 cut(s) | 397, 483, 504, 549, 629, 797, 819, 922, 991 |
| HapII | CCGG | 6 cut(s) | 358, 561, 610, 630, 829, 887 |
| HgaI | GACGC | 1 cut(s) | 308 |
| HhaI | GCGC | 8 cut(s) | 62, 77, 286, 472, 509, 588, 609, 623 |
| Hin1I | GRCGYC | 1 cut(s) | 300 |
| Hin1II | CATG | 4 cut(s) | 416, 515, 648, 1074 |
| Hin6I | GCGC | 8 cut(s) | 60, 75, 284, 470, 507, 586, 607, 621 |
| HinP1I | GCGC | 8 cut(s) | 60, 75, 284, 470, 507, 586, 607, 621 |
| HincII | GTYRAC | 2 cut(s) | 233, 600 |
| HindII | GTYRAC | 2 cut(s) | 233, 600 |
| HindIII | AAGCTT | 1 cut(s) | 93 |
| HinfI | GANTC | 4 cut(s) | 723, 822, 910, 1004 |
| HpaII | CCGG | 6 cut(s) | 358, 561, 610, 630, 829, 887 |
| HphI | GGTGA | 5 cut(s) | 155, 375, 401, 783, 1021 |
| Hpy166II | GTNNAC | 2 cut(s) | 233, 600 |
| Hpy188I | TCNGA | 6 cut(s) | 35, 91, 100, 264, 298, 710 |
| Hpy188III | TCNNGA | 6 cut(s) | 44, 512, 673, 967, 996, 1071 |
| Hpy8I | GTNNAC | 2 cut(s) | 233, 600 |
| HpyAV | CCTTC | 2 cut(s) | 473, 1002 |
| HpyCH4III | ACNGT | 2 cut(s) | 222, 1019 |
| HpyCH4IV | ACGT | 3 cut(s) | 458, 518, 1067 |
| HpyCH4V | TGCA | 5 cut(s) | 21, 644, 837, 863, 1054 |
| HpyF3I | CTNAG | 3 cut(s) | 26, 254, 766 |
| HpySE526I | ACGT | 3 cut(s) | 458, 518, 1067 |
| Hsp92I | GRCGYC | 1 cut(s) | 300 |
| Hsp92II | CATG | 4 cut(s) | 416, 515, 648, 1074 |
| HspAI | GCGC | 8 cut(s) | 60, 75, 284, 470, 507, 586, 607, 621 |
| KroI | GCCGGC | 2 cut(s) | 609, 828 |
| KroNI | GCCGGC | 2 cut(s) | 611, 830 |
| Kzo9I | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| LmnI | GCTCC | 2 cut(s) | 467, 872 |
| Lsp1109I | GCAGC | 6 cut(s) | 5, 391, 394, 498, 595, 958 |
| LweI | GCATC | 6 cut(s) | 39, 95, 268, 850, 958, 991 |
| MaeI | CTAG | 2 cut(s) | 787, 967 |
| MaeII | ACGT | 3 cut(s) | 458, 518, 1067 |
| MaeIII | GTNAC | 5 cut(s) | 222, 324, 407, 577, 927 |
| MalI | GATC | 7 cut(s) | 48, 150, 403, 436, 775, 885, 965 |
| MbiI | CCGCTC | 1 cut(s) | 575 |
| MboI | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| MboII | GAAGA | 3 cut(s) | 149, 284, 429 |
| MfeI | CAATTG | 1 cut(s) | 867 |
| MflI | RGATCY | 2 cut(s) | 401, 434 |
| MluCI | AATT | 3 cut(s) | 196, 867, 1035 |
| MlyI | GAGTC | 1 cut(s) | 998 |
| Mph1103I | ATGCAT | 1 cut(s) | 646 |
| MroNI | GCCGGC | 2 cut(s) | 609, 828 |
| MroXI | GAANNNNTTC | 1 cut(s) | 727 |
| MseI | TTAA | 1 cut(s) | 195 |
| MslI | CAYNNNNRTG | 2 cut(s) | 954, 1049 |
| MspA1I | CMGCKG | 1 cut(s) | 653 |
| MspI | CCGG | 6 cut(s) | 358, 561, 610, 630, 829, 887 |
| MspR9I | CCNGG | 9 cut(s) | 358, 376, 399, 561, 590, 625, 664, 755, 888 |
| MunI | CAATTG | 1 cut(s) | 867 |
| Mva1269I | GAATGC | 2 cut(s) | 203, 755 |
| MvaI | CCWGG | 6 cut(s) | 376, 399, 590, 625, 664, 755 |
| MvnI | CGCG | 5 cut(s) | 60, 75, 286, 507, 962 |
| NaeI | GCCGGC | 2 cut(s) | 611, 830 |
| NciI | CCSGG | 3 cut(s) | 358, 561, 888 |
| NdeII | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| NgoMIV | GCCGGC | 2 cut(s) | 609, 828 |
| NlaIII | CATG | 4 cut(s) | 416, 515, 648, 1074 |
| NlaIV | GGNNCC | 3 cut(s) | 333, 682, 1016 |
| NmeAIII | GCCGAG | 1 cut(s) | 782 |
| NmuCI | GTSAC | 3 cut(s) | 407, 577, 927 |
| NsiI | ATGCAT | 1 cut(s) | 646 |
| NspI | RCATGY | 1 cut(s) | 648 |
| PagI | TCATGA | 2 cut(s) | 511, 1070 |
| PaqCI | CACCTGC | 1 cut(s) | 704 |
| PceI | AGGCCT | 1 cut(s) | 922 |
| PctI | GAATGC | 2 cut(s) | 203, 755 |
| PdiI | GCCGGC | 2 cut(s) | 611, 830 |
| PdmI | GAANNNNTTC | 1 cut(s) | 727 |
| PfeI | GAWTC | 3 cut(s) | 723, 822, 910 |
| PflFI | GACNNNGTC | 1 cut(s) | 1066 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 851 |
| PleI | GAGTC | 1 cut(s) | 998 |
| PpsI | GAGTC | 1 cut(s) | 998 |
| PshBI | ATTAAT | 1 cut(s) | 195 |
| Psp6I | CCWGG | 6 cut(s) | 374, 397, 588, 623, 662, 753 |
| PspFI | CCCAGC | 4 cut(s) | 104, 280, 603, 844 |
| PspGI | CCWGG | 6 cut(s) | 374, 397, 588, 623, 662, 753 |
| PspN4I | GGNNCC | 3 cut(s) | 333, 682, 1016 |
| PspPI | GGNCC | 4 cut(s) | 35, 627, 681, 1015 |
| PsuI | RGATCY | 2 cut(s) | 401, 434 |
| PsyI | GACNNNGTC | 1 cut(s) | 1066 |
| RsaI | GTAC | 3 cut(s) | 417, 637, 649 |
| RsaNI | GTAC | 3 cut(s) | 416, 636, 648 |
| RseI | CAYNNNNRTG | 2 cut(s) | 954, 1049 |
| SalI | GTCGAC | 1 cut(s) | 598 |
| SaqAI | TTAA | 1 cut(s) | 195 |
| Sau3AI | GATC | 7 cut(s) | 46, 148, 401, 434, 773, 883, 963 |
| Sau96I | GGNCC | 4 cut(s) | 35, 627, 681, 1015 |
| SchI | GAGTC | 1 cut(s) | 998 |
| ScrFI | CCNGG | 9 cut(s) | 358, 376, 399, 561, 590, 625, 664, 755, 888 |
| SfaNI | GCATC | 6 cut(s) | 39, 95, 268, 850, 958, 991 |
| SinI | GGWCC | 3 cut(s) | 35, 681, 1015 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 954, 1049 |
| SmlI | CTYRAG | 1 cut(s) | 1009 |
| SmoI | CTYRAG | 1 cut(s) | 1009 |
| Sse9I | AATT | 3 cut(s) | 196, 867, 1035 |
| SseBI | AGGCCT | 1 cut(s) | 922 |
| SspMI | CTAG | 2 cut(s) | 787, 967 |
| StuI | AGGCCT | 1 cut(s) | 922 |
| StyD4I | CCNGG | 9 cut(s) | 356, 374, 397, 559, 588, 623, 662, 753, 886 |
| StyI | CCWWGG | 1 cut(s) | 786 |
| TaaI | ACNGT | 2 cut(s) | 222, 1019 |
| TaiI | ACGT | 3 cut(s) | 461, 521, 1070 |
| TaqI | TCGA | 3 cut(s) | 133, 599, 747 |
| TasI | AATT | 3 cut(s) | 196, 867, 1035 |
| TauI | GCSGC | 8 cut(s) | 65, 388, 486, 507, 549, 946, 962, 991 |
| TfiI | GAWTC | 3 cut(s) | 723, 822, 910 |
| Tru1I | TTAA | 1 cut(s) | 195 |
| Tru9I | TTAA | 1 cut(s) | 195 |
| TscAI | CASTG | 2 cut(s) | 347, 433 |
| TseFI | GTSAC | 3 cut(s) | 407, 577, 927 |
| TseI | GCWGC | 6 cut(s) | 18, 379, 382, 486, 583, 946 |
| Tsp45I | GTSAC | 3 cut(s) | 407, 577, 927 |
| TspDTI | ATGAA | 1 cut(s) | 429 |
| TspRI | CASTG | 2 cut(s) | 347, 433 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1066 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 851 |
| VpaK11BI | GGWCC | 3 cut(s) | 35, 681, 1015 |
| VspI | ATTAAT | 1 cut(s) | 195 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 170 |
| XbaI | TCTAGA | 1 cut(s) | 966 |
| XceI | RCATGY | 1 cut(s) | 648 |
| XmaJI | CCTAGG | 1 cut(s) | 786 |
| XmiI | GTMKAC | 1 cut(s) | 599 |
| XmnI | GAANNNNTTC | 1 cut(s) | 727 |
| XspI | CTAG | 2 cut(s) | 787, 967 |
| Zsp2I | ATGCAT | 1 cut(s) | 646 |