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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 62 |
| AccB1I | GGYRCC | 3 cut(s) | 142, 163, 385 |
| AccBSI | CCGCTC | 1 cut(s) | 27 |
| AccII | CGCG | 1 cut(s) | 113 |
| AciI | CCGC | 7 cut(s) | 27, 51, 78, 89, 113, 226, 348 |
| AclWI | GGATC | 1 cut(s) | 375 |
| AcoI | YGGCCR | 1 cut(s) | 114 |
| AcyI | GRCGYC | 2 cut(s) | 143, 386 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 19, 26, 123 |
| AgsI | TTSAA | 1 cut(s) | 399 |
| AluBI | AGCT | 2 cut(s) | 254, 395 |
| AluI | AGCT | 2 cut(s) | 254, 395 |
| AlwI | GGATC | 1 cut(s) | 375 |
| AoxI | GGCC | 2 cut(s) | 39, 114 |
| AspLEI | GCGC | 3 cut(s) | 113, 145, 388 |
| AspS9I | GGNCC | 2 cut(s) | 39, 345 |
| AsuC2I | CCSGG | 1 cut(s) | 20 |
| AsuHPI | GGTGA | 1 cut(s) | 34 |
| AvaII | GGWCC | 1 cut(s) | 345 |
| BanI | GGYRCC | 3 cut(s) | 142, 163, 385 |
| BauI | CACGAG | 1 cut(s) | 334 |
| BccI | CCATC | 2 cut(s) | 258, 333 |
| BceAI | ACGGC | 1 cut(s) | 102 |
| BclI | TGATCA | 1 cut(s) | 465 |
| BcnI | CCSGG | 1 cut(s) | 20 |
| BfaI | CTAG | 1 cut(s) | 215 |
| BfoI | RGCGCY | 2 cut(s) | 146, 389 |
| BfuAI | ACCTGC | 1 cut(s) | 62 |
| BisI | GCNGC | 2 cut(s) | 28, 114 |
| BlsI | GCNGC | 2 cut(s) | 29, 115 |
| Bme1390I | CCNGG | 1 cut(s) | 20 |
| Bme18I | GGWCC | 1 cut(s) | 345 |
| BmgT120I | GGNCC | 2 cut(s) | 39, 345 |
| BmiI | GGNNCC | 3 cut(s) | 144, 165, 387 |
| BmrFI | CCNGG | 1 cut(s) | 20 |
| BmsI | GCATC | 2 cut(s) | 187, 304 |
| BpuEI | CTTGAG | 1 cut(s) | 397 |
| BpuMI | CCSGG | 1 cut(s) | 20 |
| BsaBI | GATNNNNATC | 1 cut(s) | 268 |
| BsaHI | GRCGYC | 2 cut(s) | 143, 386 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 19, 26, 123 |
| Bse118I | RCCGGY | 1 cut(s) | 139 |
| Bse8I | GATNNNNATC | 1 cut(s) | 268 |
| BseGI | GGATG | 1 cut(s) | 269 |
| BseJI | GATNNNNATC | 1 cut(s) | 268 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 19, 26, 123 |
| BseRI | GAGGAG | 1 cut(s) | 400 |
| BseX3I | CGGCCG | 1 cut(s) | 114 |
| Bsh1236I | CGCG | 1 cut(s) | 113 |
| Bsh1285I | CGRYCG | 1 cut(s) | 117 |
| BshFI | GGCC | 2 cut(s) | 41, 116 |
| BshNI | GGYRCC | 3 cut(s) | 142, 163, 385 |
| BsiEI | CGRYCG | 1 cut(s) | 117 |
| BsiSI | CCGG | 4 cut(s) | 20, 117, 140, 389 |
| BslFI | GGGAC | 2 cut(s) | 139, 419 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 19, 26, 123 |
| BsmFI | GGGAC | 2 cut(s) | 139, 419 |
| BsnI | GGCC | 2 cut(s) | 41, 116 |
| Bsp143I | GATC | 2 cut(s) | 367, 465 |
| BspACI | CCGC | 7 cut(s) | 27, 51, 78, 89, 113, 226, 348 |
| BspANI | GGCC | 2 cut(s) | 41, 116 |
| BspFNI | CGCG | 1 cut(s) | 113 |
| BspHI | TCATGA | 1 cut(s) | 473 |
| BspLI | GGNNCC | 3 cut(s) | 144, 165, 387 |
| BspMI | ACCTGC | 1 cut(s) | 62 |
| BspPI | GGATC | 1 cut(s) | 375 |
| BspT107I | GGYRCC | 3 cut(s) | 142, 163, 385 |
| BsrBI | CCGCTC | 1 cut(s) | 27 |
| BsrFI | RCCGGY | 1 cut(s) | 139 |
| BssAI | RCCGGY | 1 cut(s) | 139 |
| BssMI | GATC | 2 cut(s) | 367, 465 |
| BssNI | GRCGYC | 2 cut(s) | 143, 386 |
| BssSI | CACGAG | 1 cut(s) | 334 |
| Bst2BI | CACGAG | 1 cut(s) | 334 |
| Bst4CI | ACNGT | 1 cut(s) | 193 |
| BstACI | GRCGYC | 2 cut(s) | 143, 386 |
| BstC8I | GCNNGC | 1 cut(s) | 43 |
| BstDEI | CTNAG | 1 cut(s) | 302 |
| BstF5I | GGATG | 1 cut(s) | 269 |
| BstFNI | CGCG | 1 cut(s) | 113 |
| BstH2I | RGCGCY | 2 cut(s) | 146, 389 |
| BstHHI | GCGC | 3 cut(s) | 113, 145, 388 |
| BstKTI | GATC | 2 cut(s) | 370, 468 |
| BstMBI | GATC | 2 cut(s) | 367, 465 |
| BstMCI | CGRYCG | 1 cut(s) | 117 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 392 |
| BstSCI | CCNGG | 1 cut(s) | 18 |
| BstUI | CGCG | 1 cut(s) | 113 |
| BstX2I | RGATCY | 1 cut(s) | 367 |
| BstYI | RGATCY | 1 cut(s) | 367 |
| BstZI | CGGCCG | 1 cut(s) | 114 |
| BsuRI | GGCC | 2 cut(s) | 41, 116 |
| BtsCI | GGATG | 1 cut(s) | 269 |
| BveI | ACCTGC | 1 cut(s) | 62 |
| Cac8I | GCNNGC | 1 cut(s) | 43 |
| CciI | TCATGA | 1 cut(s) | 473 |
| CfoI | GCGC | 3 cut(s) | 113, 145, 388 |
| Cfr10I | RCCGGY | 1 cut(s) | 139 |
| Cfr13I | GGNCC | 2 cut(s) | 39, 345 |
| CviAII | CATG | 1 cut(s) | 474 |
| CviJI | RGCY | 4 cut(s) | 41, 116, 254, 395 |
| CviKI_1 | RGCY | 4 cut(s) | 41, 116, 254, 395 |
| DdeI | CTNAG | 1 cut(s) | 302 |
| DinI | GGCGCC | 2 cut(s) | 144, 387 |
| DpnI | GATC | 2 cut(s) | 369, 467 |
| DpnII | GATC | 2 cut(s) | 367, 465 |
| DraI | TTTAAA | 1 cut(s) | 250 |
| EaeI | YGGCCR | 1 cut(s) | 114 |
| EagI | CGGCCG | 1 cut(s) | 114 |
| EciI | GGCGGA | 1 cut(s) | 104 |
| EclXI | CGGCCG | 1 cut(s) | 114 |
| Eco47I | GGWCC | 1 cut(s) | 345 |
| Eco52I | CGGCCG | 1 cut(s) | 114 |
| EgeI | GGCGCC | 2 cut(s) | 144, 387 |
| EheI | GGCGCC | 2 cut(s) | 144, 387 |
| FaeI | CATG | 1 cut(s) | 477 |
| FaiI | YATR | 5 cut(s) | 96, 430, 462, 464, 475 |
| FaqI | GGGAC | 2 cut(s) | 139, 419 |
| FatI | CATG | 1 cut(s) | 473 |
| FauI | CCCGC | 1 cut(s) | 219 |
| FauNDI | CATATG | 1 cut(s) | 462 |
| FbaI | TGATCA | 1 cut(s) | 465 |
| Fnu4HI | GCNGC | 2 cut(s) | 28, 114 |
| FokI | GGATG | 1 cut(s) | 276 |
| Fsp4HI | GCNGC | 2 cut(s) | 28, 114 |
| FspBI | CTAG | 1 cut(s) | 215 |
| GlaI | GCGC | 3 cut(s) | 112, 144, 387 |
| GluI | GCNGC | 2 cut(s) | 28, 114 |
| HaeII | RGCGCY | 2 cut(s) | 146, 389 |
| HaeIII | GGCC | 2 cut(s) | 41, 116 |
| HapII | CCGG | 4 cut(s) | 20, 117, 140, 389 |
| HhaI | GCGC | 3 cut(s) | 113, 145, 388 |
| Hin1I | GRCGYC | 2 cut(s) | 143, 386 |
| Hin1II | CATG | 1 cut(s) | 477 |
| Hin6I | GCGC | 3 cut(s) | 111, 143, 386 |
| HinP1I | GCGC | 3 cut(s) | 111, 143, 386 |
| HincII | GTYRAC | 1 cut(s) | 285 |
| HindII | GTYRAC | 1 cut(s) | 285 |
| HindIII | AAGCTT | 1 cut(s) | 393 |
| HinfI | GANTC | 4 cut(s) | 34, 81, 314, 407 |
| HpaII | CCGG | 4 cut(s) | 20, 117, 140, 389 |
| HphI | GGTGA | 1 cut(s) | 34 |
| Hpy166II | GTNNAC | 1 cut(s) | 285 |
| Hpy188III | TCNNGA | 5 cut(s) | 334, 357, 365, 371, 474 |
| Hpy8I | GTNNAC | 1 cut(s) | 285 |
| Hpy99I | CGWCG | 1 cut(s) | 314 |
| HpyCH4III | ACNGT | 1 cut(s) | 193 |
| HpyCH4V | TGCA | 1 cut(s) | 65 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 392 |
| HpyF3I | CTNAG | 1 cut(s) | 302 |
| Hsp92I | GRCGYC | 2 cut(s) | 143, 386 |
| Hsp92II | CATG | 1 cut(s) | 477 |
| HspAI | GCGC | 3 cut(s) | 111, 143, 386 |
| KasI | GGCGCC | 2 cut(s) | 142, 385 |
| Ksp22I | TGATCA | 1 cut(s) | 465 |
| Kzo9I | GATC | 2 cut(s) | 367, 465 |
| LpnPI | CCDG | 8 cut(s) | 33, 55, 57, 130, 153, 246, 293, 402 |
| LweI | GCATC | 2 cut(s) | 187, 304 |
| MaeI | CTAG | 1 cut(s) | 215 |
| MaeIII | GTNAC | 1 cut(s) | 331 |
| MalI | GATC | 2 cut(s) | 369, 467 |
| MbiI | CCGCTC | 1 cut(s) | 27 |
| MboI | GATC | 2 cut(s) | 367, 465 |
| MboII | GAAGA | 1 cut(s) | 248 |
| MfeI | CAATTG | 3 cut(s) | 57, 327, 444 |
| MflI | RGATCY | 1 cut(s) | 367 |
| MluCI | AATT | 5 cut(s) | 57, 100, 230, 327, 444 |
| Mly113I | GGCGCC | 2 cut(s) | 143, 386 |
| MlyI | GAGTC | 3 cut(s) | 75, 323, 401 |
| MnlI | CCTC | 4 cut(s) | 58, 115, 336, 421 |
| MseI | TTAA | 3 cut(s) | 249, 320, 438 |
| MslI | CAYNNNNRTG | 1 cut(s) | 358 |
| MspI | CCGG | 4 cut(s) | 20, 117, 140, 389 |
| MspR9I | CCNGG | 1 cut(s) | 20 |
| MunI | CAATTG | 3 cut(s) | 57, 327, 444 |
| MvnI | CGCG | 1 cut(s) | 113 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 392 |
| NarI | GGCGCC | 2 cut(s) | 143, 386 |
| NciI | CCSGG | 1 cut(s) | 20 |
| NdeI | CATATG | 1 cut(s) | 462 |
| NdeII | GATC | 2 cut(s) | 367, 465 |
| NlaIII | CATG | 1 cut(s) | 477 |
| NlaIV | GGNNCC | 3 cut(s) | 144, 165, 387 |
| NmuCI | GTSAC | 1 cut(s) | 331 |
| PagI | TCATGA | 1 cut(s) | 473 |
| PfeI | GAWTC | 1 cut(s) | 34 |
| PkrI | GCNGC | 2 cut(s) | 29, 115 |
| PleI | GAGTC | 3 cut(s) | 75, 322, 401 |
| PluTI | GGCGCC | 2 cut(s) | 146, 389 |
| PpsI | GAGTC | 3 cut(s) | 75, 322, 401 |
| PspN4I | GGNNCC | 3 cut(s) | 144, 165, 387 |
| PspPI | GGNCC | 2 cut(s) | 39, 345 |
| PsuI | RGATCY | 1 cut(s) | 367 |
| RseI | CAYNNNNRTG | 1 cut(s) | 358 |
| SaqAI | TTAA | 3 cut(s) | 249, 320, 438 |
| SatI | GCNGC | 2 cut(s) | 28, 114 |
| Sau3AI | GATC | 2 cut(s) | 367, 465 |
| Sau96I | GGNCC | 2 cut(s) | 39, 345 |
| SchI | GAGTC | 3 cut(s) | 75, 323, 401 |
| ScrFI | CCNGG | 1 cut(s) | 20 |
| SetI | ASST | 9 cut(s) | 50, 76, 178, 209, 245, 256, 308, 397, 438 |
| SfaNI | GCATC | 2 cut(s) | 187, 304 |
| SfoI | GGCGCC | 2 cut(s) | 144, 387 |
| SgrAI | CRCCGGYG | 1 cut(s) | 139 |
| SinI | GGWCC | 1 cut(s) | 345 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 358 |
| SmlI | CTYRAG | 1 cut(s) | 412 |
| SmoI | CTYRAG | 1 cut(s) | 412 |
| Sse9I | AATT | 5 cut(s) | 57, 100, 230, 327, 444 |
| SsiI | CCGC | 7 cut(s) | 27, 51, 78, 89, 113, 226, 348 |
| SspDI | GGCGCC | 2 cut(s) | 142, 385 |
| SspMI | CTAG | 1 cut(s) | 215 |
| StyD4I | CCNGG | 1 cut(s) | 18 |
| TaaI | ACNGT | 1 cut(s) | 193 |
| TasI | AATT | 5 cut(s) | 57, 100, 230, 327, 444 |
| TauI | GCSGC | 2 cut(s) | 30, 116 |
| TfiI | GAWTC | 1 cut(s) | 34 |
| Tru1I | TTAA | 3 cut(s) | 249, 320, 438 |
| Tru9I | TTAA | 3 cut(s) | 249, 320, 438 |
| TseFI | GTSAC | 1 cut(s) | 331 |
| Tsp45I | GTSAC | 1 cut(s) | 331 |
| TspDTI | ATGAA | 1 cut(s) | 93 |
| VpaK11BI | GGWCC | 1 cut(s) | 345 |
| XspI | CTAG | 1 cut(s) | 215 |