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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 307 |
| Acc36I | ACCTGC | 1 cut(s) | 307 |
| AccB1I | GGYRCC | 1 cut(s) | 412 |
| AciI | CCGC | 4 cut(s) | 15, 293, 323, 453 |
| AclWI | GGATC | 1 cut(s) | 105 |
| AcyI | GRCGYC | 1 cut(s) | 447 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 210 |
| AluBI | AGCT | 3 cut(s) | 197, 334, 480 |
| AluI | AGCT | 3 cut(s) | 197, 334, 480 |
| Alw26I | GTCTC | 1 cut(s) | 24 |
| AlwI | GGATC | 1 cut(s) | 105 |
| ApeKI | GCWGC | 3 cut(s) | 104, 222, 334 |
| AspS9I | GGNCC | 1 cut(s) | 284 |
| AsuC2I | CCSGG | 1 cut(s) | 95 |
| AsuNHI | GCTAGC | 1 cut(s) | 5 |
| AvaII | GGWCC | 1 cut(s) | 284 |
| BanI | GGYRCC | 1 cut(s) | 412 |
| BbvCI | CCTCAGC | 1 cut(s) | 57 |
| BbvI | GCAGC | 3 cut(s) | 91, 234, 321 |
| BccI | CCATC | 1 cut(s) | 85 |
| BcnI | CCSGG | 1 cut(s) | 95 |
| BcoDI | GTCTC | 1 cut(s) | 24 |
| BfaI | CTAG | 4 cut(s) | 6, 272, 432, 481 |
| BfmI | CTRYAG | 1 cut(s) | 299 |
| BfuAI | ACCTGC | 1 cut(s) | 307 |
| BisI | GCNGC | 4 cut(s) | 16, 105, 223, 335 |
| BlsI | GCNGC | 4 cut(s) | 17, 106, 224, 336 |
| Bme1390I | CCNGG | 1 cut(s) | 95 |
| Bme18I | GGWCC | 1 cut(s) | 284 |
| BmgT120I | GGNCC | 1 cut(s) | 284 |
| BmiI | GGNNCC | 1 cut(s) | 414 |
| BmrFI | CCNGG | 1 cut(s) | 95 |
| BmtI | GCTAGC | 1 cut(s) | 9 |
| Bpu10I | CCTNAGC | 1 cut(s) | 57 |
| BpuMI | CCSGG | 1 cut(s) | 95 |
| BsaHI | GRCGYC | 1 cut(s) | 447 |
| BsaI | GGTCTC | 1 cut(s) | 24 |
| BsaJI | CCNNGG | 1 cut(s) | 123 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 21, 51 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 210 |
| Bse118I | RCCGGY | 1 cut(s) | 359 |
| Bse1I | ACTGG | 2 cut(s) | 382, 468 |
| BseDI | CCNNGG | 1 cut(s) | 123 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 210 |
| BseMII | CTCAG | 2 cut(s) | 71, 212 |
| BseNI | ACTGG | 2 cut(s) | 382, 468 |
| BseXI | GCAGC | 3 cut(s) | 91, 234, 321 |
| BshNI | GGYRCC | 1 cut(s) | 412 |
| BsiSI | CCGG | 2 cut(s) | 94, 360 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 210 |
| BsmAI | GTCTC | 1 cut(s) | 24 |
| Bso31I | GGTCTC | 1 cut(s) | 24 |
| Bsp143I | GATC | 3 cut(s) | 40, 97, 151 |
| BspACI | CCGC | 4 cut(s) | 15, 293, 323, 453 |
| BspCNI | CTCAG | 2 cut(s) | 70, 211 |
| BspLI | GGNNCC | 1 cut(s) | 414 |
| BspMI | ACCTGC | 1 cut(s) | 307 |
| BspOI | GCTAGC | 1 cut(s) | 9 |
| BspPI | GGATC | 1 cut(s) | 105 |
| BspT107I | GGYRCC | 1 cut(s) | 412 |
| BspTNI | GGTCTC | 1 cut(s) | 24 |
| BsrFI | RCCGGY | 1 cut(s) | 359 |
| BsrI | ACTGG | 2 cut(s) | 382, 468 |
| BssAI | RCCGGY | 1 cut(s) | 359 |
| BssECI | CCNNGG | 1 cut(s) | 123 |
| BssMI | GATC | 3 cut(s) | 40, 97, 151 |
| BssNI | GRCGYC | 1 cut(s) | 447 |
| BssT1I | CCWWGG | 1 cut(s) | 123 |
| Bst4CI | ACNGT | 1 cut(s) | 85 |
| BstACI | GRCGYC | 1 cut(s) | 447 |
| BstC8I | GCNNGC | 2 cut(s) | 7, 390 |
| BstDEI | CTNAG | 2 cut(s) | 57, 198 |
| BstKTI | GATC | 3 cut(s) | 43, 100, 154 |
| BstMAI | GTCTC | 1 cut(s) | 24 |
| BstMBI | GATC | 3 cut(s) | 40, 97, 151 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 15 |
| BstNSI | RCATGY | 2 cut(s) | 388, 430 |
| BstSCI | CCNGG | 1 cut(s) | 93 |
| BstSFI | CTRYAG | 1 cut(s) | 299 |
| BstV1I | GCAGC | 3 cut(s) | 91, 234, 321 |
| BstX2I | RGATCY | 1 cut(s) | 97 |
| BstYI | RGATCY | 1 cut(s) | 97 |
| BtsIMutI | CAGTG | 1 cut(s) | 361 |
| BveI | ACCTGC | 1 cut(s) | 307 |
| Cac8I | GCNNGC | 2 cut(s) | 7, 390 |
| Cfr10I | RCCGGY | 1 cut(s) | 359 |
| Cfr13I | GGNCC | 1 cut(s) | 284 |
| CseI | GACGC | 1 cut(s) | 455 |
| CviAII | CATG | 2 cut(s) | 385, 427 |
| CviJI | RGCY | 7 cut(s) | 5, 47, 61, 122, 197, 334, 480 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 47, 61, 122, 197, 334, 480 |
| DdeI | CTNAG | 2 cut(s) | 57, 198 |
| DpnI | GATC | 3 cut(s) | 42, 99, 153 |
| DpnII | GATC | 3 cut(s) | 40, 97, 151 |
| EciI | GGCGGA | 1 cut(s) | 338 |
| Eco130I | CCWWGG | 1 cut(s) | 123 |
| Eco31I | GGTCTC | 1 cut(s) | 24 |
| Eco47I | GGWCC | 1 cut(s) | 284 |
| EcoT14I | CCWWGG | 1 cut(s) | 123 |
| ErhI | CCWWGG | 1 cut(s) | 123 |
| FaeI | CATG | 2 cut(s) | 388, 430 |
| FaiI | YATR | 4 cut(s) | 110, 342, 386, 428 |
| FatI | CATG | 2 cut(s) | 384, 426 |
| Fnu4HI | GCNGC | 4 cut(s) | 16, 105, 223, 335 |
| Fsp4HI | GCNGC | 4 cut(s) | 16, 105, 223, 335 |
| FspBI | CTAG | 4 cut(s) | 6, 272, 432, 481 |
| GluI | GCNGC | 4 cut(s) | 16, 105, 223, 335 |
| HapII | CCGG | 2 cut(s) | 94, 360 |
| HgaI | GACGC | 1 cut(s) | 455 |
| Hin1I | GRCGYC | 1 cut(s) | 447 |
| Hin1II | CATG | 2 cut(s) | 388, 430 |
| HincII | GTYRAC | 3 cut(s) | 88, 365, 445 |
| HindII | GTYRAC | 3 cut(s) | 88, 365, 445 |
| HinfI | GANTC | 2 cut(s) | 182, 326 |
| HpaII | CCGG | 2 cut(s) | 94, 360 |
| Hpy166II | GTNNAC | 3 cut(s) | 88, 365, 445 |
| Hpy188III | TCNNGA | 2 cut(s) | 155, 217 |
| Hpy8I | GTNNAC | 3 cut(s) | 88, 365, 445 |
| HpyAV | CCTTC | 1 cut(s) | 169 |
| HpyCH4III | ACNGT | 1 cut(s) | 85 |
| HpyCH4V | TGCA | 3 cut(s) | 136, 337, 392 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 15 |
| HpyF3I | CTNAG | 2 cut(s) | 57, 198 |
| Hsp92I | GRCGYC | 1 cut(s) | 447 |
| Hsp92II | CATG | 2 cut(s) | 388, 430 |
| Kzo9I | GATC | 3 cut(s) | 40, 97, 151 |
| LmnI | GCTCC | 1 cut(s) | 477 |
| LpnPI | CCDG | 8 cut(s) | 81, 107, 196, 287, 302, 363, 373, 449 |
| Lsp1109I | GCAGC | 3 cut(s) | 91, 234, 321 |
| MaeI | CTAG | 4 cut(s) | 6, 272, 432, 481 |
| MalI | GATC | 3 cut(s) | 42, 99, 153 |
| MboI | GATC | 3 cut(s) | 40, 97, 151 |
| MflI | RGATCY | 1 cut(s) | 97 |
| MluCI | AATT | 1 cut(s) | 346 |
| MlyI | GAGTC | 1 cut(s) | 320 |
| MmeI | TCCRAC | 1 cut(s) | 7 |
| MnlI | CCTC | 3 cut(s) | 13, 66, 365 |
| MspA1I | CMGCKG | 1 cut(s) | 334 |
| MspI | CCGG | 2 cut(s) | 94, 360 |
| MspR9I | CCNGG | 1 cut(s) | 95 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 15 |
| NciI | CCSGG | 1 cut(s) | 95 |
| NdeII | GATC | 3 cut(s) | 40, 97, 151 |
| NheI | GCTAGC | 1 cut(s) | 5 |
| NlaIII | CATG | 2 cut(s) | 388, 430 |
| NlaIV | GGNNCC | 1 cut(s) | 414 |
| NspI | RCATGY | 2 cut(s) | 388, 430 |
| PaqCI | CACCTGC | 1 cut(s) | 307 |
| PfeI | GAWTC | 1 cut(s) | 182 |
| PkrI | GCNGC | 4 cut(s) | 17, 106, 224, 336 |
| PleI | GAGTC | 1 cut(s) | 320 |
| PpsI | GAGTC | 1 cut(s) | 320 |
| PspN4I | GGNNCC | 1 cut(s) | 414 |
| PspPI | GGNCC | 1 cut(s) | 284 |
| PsuI | RGATCY | 1 cut(s) | 97 |
| PvuII | CAGCTG | 1 cut(s) | 334 |
| SatI | GCNGC | 4 cut(s) | 16, 105, 223, 335 |
| Sau3AI | GATC | 3 cut(s) | 40, 97, 151 |
| Sau96I | GGNCC | 1 cut(s) | 284 |
| SchI | GAGTC | 1 cut(s) | 320 |
| ScrFI | CCNGG | 1 cut(s) | 95 |
| SetI | ASST | 5 cut(s) | 199, 306, 321, 336, 482 |
| SfcI | CTRYAG | 1 cut(s) | 299 |
| SinI | GGWCC | 1 cut(s) | 284 |
| Sse9I | AATT | 1 cut(s) | 346 |
| SsiI | CCGC | 4 cut(s) | 15, 293, 323, 453 |
| SspMI | CTAG | 4 cut(s) | 6, 272, 432, 481 |
| StyD4I | CCNGG | 1 cut(s) | 93 |
| StyI | CCWWGG | 1 cut(s) | 123 |
| TaaI | ACNGT | 1 cut(s) | 85 |
| TaqI | TCGA | 1 cut(s) | 154 |
| TaqII | GACCGA | 2 cut(s) | 301, 451 |
| TasI | AATT | 1 cut(s) | 346 |
| TauI | GCSGC | 1 cut(s) | 18 |
| TfiI | GAWTC | 1 cut(s) | 182 |
| TscAI | CASTG | 1 cut(s) | 361 |
| TseI | GCWGC | 3 cut(s) | 104, 222, 334 |
| TspDTI | ATGAA | 1 cut(s) | 339 |
| TspRI | CASTG | 1 cut(s) | 361 |
| VpaK11BI | GGWCC | 1 cut(s) | 284 |
| XceI | RCATGY | 2 cut(s) | 388, 430 |
| XspI | CTAG | 4 cut(s) | 6, 272, 432, 481 |