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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 74 |
| AciI | CCGC | 4 cut(s) | 72, 74, 231, 254 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 1 cut(s) | 231 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| AgsI | TTSAA | 1 cut(s) | 284 |
| AjnI | CCWGG | 2 cut(s) | 147, 159 |
| AluBI | AGCT | 2 cut(s) | 92, 280 |
| AluI | AGCT | 2 cut(s) | 92, 280 |
| Alw26I | GTCTC | 1 cut(s) | 169 |
| AlwI | GGATC | 1 cut(s) | 231 |
| AoxI | GGCC | 2 cut(s) | 303, 309 |
| ApeKI | GCWGC | 1 cut(s) | 201 |
| AspS9I | GGNCC | 1 cut(s) | 256 |
| AsuC2I | CCSGG | 1 cut(s) | 221 |
| AvaII | GGWCC | 1 cut(s) | 256 |
| AxyI | CCTNAGG | 1 cut(s) | 135 |
| BanII | GRGCYC | 1 cut(s) | 155 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 213 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 2 cut(s) | 149, 161 |
| BcnI | CCSGG | 1 cut(s) | 221 |
| BcoDI | GTCTC | 1 cut(s) | 169 |
| BfmI | CTRYAG | 1 cut(s) | 180 |
| BisI | GCNGC | 2 cut(s) | 75, 202 |
| BlsI | GCNGC | 2 cut(s) | 76, 203 |
| Bme1390I | CCNGG | 3 cut(s) | 149, 161, 221 |
| Bme18I | GGWCC | 1 cut(s) | 256 |
| BmgT120I | GGNCC | 1 cut(s) | 256 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 3 cut(s) | 149, 161, 221 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 221 |
| BsaI | GGTCTC | 1 cut(s) | 169 |
| BsaJI | CCNNGG | 5 cut(s) | 72, 147, 148, 159, 306 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| Bse21I | CCTNAGG | 1 cut(s) | 135 |
| BseBI | CCWGG | 2 cut(s) | 149, 161 |
| BseDI | CCNNGG | 5 cut(s) | 72, 147, 148, 159, 306 |
| BseGI | GGATG | 2 cut(s) | 58, 155 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 1 cut(s) | 213 |
| BseYI | CCCAGC | 1 cut(s) | 88 |
| Bsh1236I | CGCG | 1 cut(s) | 74 |
| BshFI | GGCC | 2 cut(s) | 305, 311 |
| BsiSI | CCGG | 1 cut(s) | 220 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 265 |
| BsmAI | GTCTC | 1 cut(s) | 169 |
| BsnI | GGCC | 2 cut(s) | 305, 311 |
| Bso31I | GGTCTC | 1 cut(s) | 169 |
| Bsp1286I | GDGCHC | 1 cut(s) | 155 |
| Bsp143I | GATC | 2 cut(s) | 40, 223 |
| BspACI | CCGC | 4 cut(s) | 72, 74, 231, 254 |
| BspANI | GGCC | 2 cut(s) | 305, 311 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 1 cut(s) | 74 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspPI | GGATC | 1 cut(s) | 231 |
| BspTNI | GGTCTC | 1 cut(s) | 169 |
| BssECI | CCNNGG | 5 cut(s) | 72, 147, 148, 159, 306 |
| BssMI | GATC | 2 cut(s) | 40, 223 |
| Bst2UI | CCWGG | 2 cut(s) | 149, 161 |
| Bst4CI | ACNGT | 1 cut(s) | 181 |
| BstC8I | GCNNGC | 1 cut(s) | 36 |
| BstDEI | CTNAG | 2 cut(s) | 27, 135 |
| BstDSI | CCRYGG | 1 cut(s) | 72 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 2 cut(s) | 58, 155 |
| BstFNI | CGCG | 1 cut(s) | 74 |
| BstKTI | GATC | 2 cut(s) | 43, 226 |
| BstMAI | GTCTC | 1 cut(s) | 169 |
| BstMBI | GATC | 2 cut(s) | 40, 223 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| BstNI | CCWGG | 2 cut(s) | 149, 161 |
| BstSCI | CCNGG | 3 cut(s) | 147, 159, 219 |
| BstSFI | CTRYAG | 1 cut(s) | 180 |
| BstUI | CGCG | 1 cut(s) | 74 |
| BstV1I | GCAGC | 1 cut(s) | 213 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| Bsu36I | CCTNAGG | 1 cut(s) | 135 |
| BsuRI | GGCC | 2 cut(s) | 305, 311 |
| BtgI | CCRYGG | 1 cut(s) | 72 |
| BtsCI | GGATG | 2 cut(s) | 58, 155 |
| Cac8I | GCNNGC | 1 cut(s) | 36 |
| Cfr13I | GGNCC | 1 cut(s) | 256 |
| Cfr42I | CCGCGG | 1 cut(s) | 75 |
| CviJI | RGCY | 7 cut(s) | 77, 92, 153, 219, 280, 305, 311 |
| CviKI_1 | RGCY | 7 cut(s) | 77, 92, 153, 219, 280, 305, 311 |
| DdeI | CTNAG | 2 cut(s) | 27, 135 |
| DpnI | GATC | 2 cut(s) | 42, 225 |
| DpnII | GATC | 2 cut(s) | 40, 223 |
| EciI | GGCGGA | 1 cut(s) | 269 |
| Eco147I | AGGCCT | 1 cut(s) | 311 |
| Eco24I | GRGCYC | 1 cut(s) | 155 |
| Eco31I | GGTCTC | 1 cut(s) | 169 |
| Eco47I | GGWCC | 1 cut(s) | 256 |
| Eco81I | CCTNAGG | 1 cut(s) | 135 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoRII | CCWGG | 2 cut(s) | 147, 159 |
| EcoT38I | GRGCYC | 1 cut(s) | 155 |
| FaiI | YATR | 2 cut(s) | 236, 240 |
| FauI | CCCGC | 1 cut(s) | 79 |
| Fnu4HI | GCNGC | 2 cut(s) | 75, 202 |
| FokI | GGATG | 2 cut(s) | 45, 142 |
| FriOI | GRGCYC | 1 cut(s) | 155 |
| Fsp4HI | GCNGC | 2 cut(s) | 75, 202 |
| GluI | GCNGC | 2 cut(s) | 75, 202 |
| GsaI | CCCAGC | 1 cut(s) | 92 |
| HaeIII | GGCC | 2 cut(s) | 305, 311 |
| HapII | CCGG | 1 cut(s) | 220 |
| HincII | GTYRAC | 4 cut(s) | 52, 97, 103, 130 |
| HindII | GTYRAC | 4 cut(s) | 52, 97, 103, 130 |
| HpaI | GTTAAC | 2 cut(s) | 97, 103 |
| HpaII | CCGG | 1 cut(s) | 220 |
| Hpy166II | GTNNAC | 4 cut(s) | 52, 97, 103, 130 |
| Hpy8I | GTNNAC | 4 cut(s) | 52, 97, 103, 130 |
| HpyAV | CCTTC | 1 cut(s) | 295 |
| HpyCH4III | ACNGT | 1 cut(s) | 181 |
| HpyCH4IV | ACGT | 1 cut(s) | 99 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 135 |
| HpySE526I | ACGT | 1 cut(s) | 99 |
| KspAI | GTTAAC | 2 cut(s) | 97, 103 |
| KspI | CCGCGG | 1 cut(s) | 75 |
| Kzo9I | GATC | 2 cut(s) | 40, 223 |
| LmnI | GCTCC | 2 cut(s) | 31, 277 |
| LpnPI | CCDG | 8 cut(s) | 102, 110, 134, 146, 161, 173, 188, 233 |
| Lsp1109I | GCAGC | 1 cut(s) | 213 |
| MaeII | ACGT | 1 cut(s) | 99 |
| MalI | GATC | 2 cut(s) | 42, 225 |
| MboI | GATC | 2 cut(s) | 40, 223 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 1 cut(s) | 155 |
| MnlI | CCTC | 3 cut(s) | 22, 181, 301 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 92 |
| MspI | CCGG | 1 cut(s) | 220 |
| MspR9I | CCNGG | 3 cut(s) | 149, 161, 221 |
| MvaI | CCWGG | 2 cut(s) | 149, 161 |
| MvnI | CGCG | 1 cut(s) | 74 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 207, 216 |
| NciI | CCSGG | 1 cut(s) | 221 |
| NdeII | GATC | 2 cut(s) | 40, 223 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| PasI | CCCWGGG | 1 cut(s) | 148 |
| PceI | AGGCCT | 1 cut(s) | 311 |
| PkrI | GCNGC | 2 cut(s) | 76, 203 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| Psp6I | CCWGG | 2 cut(s) | 147, 159 |
| PspFI | CCCAGC | 1 cut(s) | 88 |
| PspGI | CCWGG | 2 cut(s) | 147, 159 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 1 cut(s) | 256 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| PvuII | CAGCTG | 1 cut(s) | 92 |
| SacII | CCGCGG | 1 cut(s) | 75 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 2 cut(s) | 75, 202 |
| Sau3AI | GATC | 2 cut(s) | 40, 223 |
| Sau96I | GGNCC | 1 cut(s) | 256 |
| ScrFI | CCNGG | 3 cut(s) | 149, 161, 221 |
| SduI | GDGCHC | 1 cut(s) | 155 |
| SetI | ASST | 6 cut(s) | 94, 102, 141, 165, 261, 282 |
| SfcI | CTRYAG | 1 cut(s) | 180 |
| Sfr303I | CCGCGG | 1 cut(s) | 75 |
| SgrBI | CCGCGG | 1 cut(s) | 75 |
| SinI | GGWCC | 1 cut(s) | 256 |
| SseBI | AGGCCT | 1 cut(s) | 311 |
| SsiI | CCGC | 4 cut(s) | 72, 74, 231, 254 |
| StuI | AGGCCT | 1 cut(s) | 311 |
| StyD4I | CCNGG | 3 cut(s) | 147, 159, 219 |
| TaaI | ACNGT | 1 cut(s) | 181 |
| TaiI | ACGT | 1 cut(s) | 102 |
| TauI | GCSGC | 1 cut(s) | 77 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TseI | GCWGC | 1 cut(s) | 201 |
| VpaK11BI | GGWCC | 1 cut(s) | 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |