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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 301 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 797 |
| Acc36I | ACCTGC | 2 cut(s) | 301, 451 |
| Acc65I | GGTACC | 2 cut(s) | 436, 463 |
| AccB1I | GGYRCC | 3 cut(s) | 436, 463, 662 |
| AccBSI | CCGCTC | 1 cut(s) | 224 |
| AccI | GTMKAC | 1 cut(s) | 415 |
| AccII | CGCG | 1 cut(s) | 397 |
| AclI | AACGTT | 1 cut(s) | 90 |
| AclWI | GGATC | 3 cut(s) | 48, 718, 731 |
| AcoI | YGGCCR | 1 cut(s) | 315 |
| AcsI | RAATTY | 1 cut(s) | 332 |
| AcuI | CTGAAG | 1 cut(s) | 721 |
| AcyI | GRCGYC | 1 cut(s) | 663 |
| AfaI | GTAC | 3 cut(s) | 438, 444, 465 |
| AgsI | TTSAA | 3 cut(s) | 109, 338, 817 |
| AjnI | CCWGG | 8 cut(s) | 138, 150, 187, 205, 210, 574, 698, 719 |
| AluBI | AGCT | 1 cut(s) | 235 |
| AluI | AGCT | 1 cut(s) | 235 |
| Alw21I | GWGCWC | 2 cut(s) | 103, 404 |
| Alw26I | GTCTC | 2 cut(s) | 173, 586 |
| AlwI | GGATC | 3 cut(s) | 48, 718, 731 |
| AoxI | GGCC | 6 cut(s) | 208, 300, 315, 496, 582, 707 |
| ApaI | GGGCCC | 2 cut(s) | 500, 586 |
| ApeKI | GCWGC | 2 cut(s) | 192, 625 |
| ApoI | RAATTY | 1 cut(s) | 332 |
| Asp718I | GGTACC | 2 cut(s) | 436, 463 |
| AspLEI | GCGC | 2 cut(s) | 382, 665 |
| AspS9I | GGNCC | 9 cut(s) | 154, 214, 247, 301, 496, 497, 582, 583, 707 |
| AsuC2I | CCSGG | 1 cut(s) | 650 |
| AsuHPI | GGTGA | 2 cut(s) | 211, 706 |
| AvaII | GGWCC | 3 cut(s) | 154, 214, 247 |
| BaeGI | GKGCMC | 2 cut(s) | 500, 586 |
| BalI | TGGCCA | 1 cut(s) | 317 |
| BamHI | GGATCC | 1 cut(s) | 723 |
| BanI | GGYRCC | 3 cut(s) | 436, 463, 662 |
| BanII | GRGCYC | 4 cut(s) | 138, 146, 500, 586 |
| Bbv12I | GWGCWC | 2 cut(s) | 103, 404 |
| BbvCI | CCTCAGC | 3 cut(s) | 27, 172, 297 |
| BbvI | GCAGC | 2 cut(s) | 204, 637 |
| BccI | CCATC | 3 cut(s) | 66, 517, 521 |
| BceAI | ACGGC | 1 cut(s) | 81 |
| BciT130I | CCWGG | 8 cut(s) | 140, 152, 189, 207, 212, 576, 700, 721 |
| BcnI | CCSGG | 1 cut(s) | 650 |
| BcoDI | GTCTC | 2 cut(s) | 173, 586 |
| BfoI | RGCGCY | 1 cut(s) | 666 |
| BfuAI | ACCTGC | 2 cut(s) | 301, 451 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 207 |
| BisI | GCNGC | 2 cut(s) | 193, 626 |
| BlsI | GCNGC | 2 cut(s) | 194, 627 |
| Bme1390I | CCNGG | 9 cut(s) | 140, 152, 189, 207, 212, 576, 650, 700, 721 |
| Bme18I | GGWCC | 3 cut(s) | 154, 214, 247 |
| BmgT120I | GGNCC | 9 cut(s) | 154, 214, 247, 301, 496, 497, 582, 583, 707 |
| BmiI | GGNNCC | 9 cut(s) | 135, 156, 438, 465, 498, 583, 584, 664, 725 |
| BmrFI | CCNGG | 9 cut(s) | 140, 152, 189, 207, 212, 576, 650, 700, 721 |
| BmsI | GCATC | 1 cut(s) | 447 |
| BpmI | CTGGAG | 2 cut(s) | 558, 610 |
| Bpu10I | CCTNAGC | 4 cut(s) | 27, 125, 172, 297 |
| BpuMI | CCSGG | 1 cut(s) | 650 |
| BsaHI | GRCGYC | 1 cut(s) | 663 |
| BsaI | GGTCTC | 1 cut(s) | 173 |
| BsaJI | CCNNGG | 4 cut(s) | 63, 138, 139, 150 |
| Bse118I | RCCGGY | 2 cut(s) | 115, 425 |
| Bse1I | ACTGG | 1 cut(s) | 246 |
| BseBI | CCWGG | 8 cut(s) | 140, 152, 189, 207, 212, 576, 700, 721 |
| BseDI | CCNNGG | 4 cut(s) | 63, 138, 139, 150 |
| BseGI | GGATG | 3 cut(s) | 58, 146, 513 |
| BseMII | CTCAG | 3 cut(s) | 18, 186, 288 |
| BseNI | ACTGG | 1 cut(s) | 246 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 2 cut(s) | 500, 586 |
| BseXI | GCAGC | 2 cut(s) | 204, 637 |
| BseYI | CCCAGC | 1 cut(s) | 376 |
| BsgI | GTGCAG | 1 cut(s) | 304 |
| Bsh1236I | CGCG | 1 cut(s) | 397 |
| BshFI | GGCC | 6 cut(s) | 210, 302, 317, 498, 584, 709 |
| BshNI | GGYRCC | 3 cut(s) | 436, 463, 662 |
| BsiHKAI | GWGCWC | 2 cut(s) | 103, 404 |
| BsiSI | CCGG | 3 cut(s) | 116, 426, 649 |
| BslFI | GGGAC | 1 cut(s) | 140 |
| BsmAI | GTCTC | 2 cut(s) | 173, 586 |
| BsmBI | CGTCTC | 1 cut(s) | 586 |
| BsmFI | GGGAC | 1 cut(s) | 140 |
| BsnI | GGCC | 6 cut(s) | 210, 302, 317, 498, 584, 709 |
| Bso31I | GGTCTC | 1 cut(s) | 173 |
| Bsp120I | GGGCCC | 2 cut(s) | 496, 582 |
| Bsp1286I | GDGCHC | 6 cut(s) | 103, 138, 146, 404, 500, 586 |
| Bsp143I | GATC | 3 cut(s) | 40, 723, 740 |
| BspANI | GGCC | 6 cut(s) | 210, 302, 317, 498, 584, 709 |
| BspCNI | CTCAG | 3 cut(s) | 19, 185, 289 |
| BspFNI | CGCG | 1 cut(s) | 397 |
| BspLI | GGNNCC | 9 cut(s) | 135, 156, 438, 465, 498, 583, 584, 664, 725 |
| BspMI | ACCTGC | 2 cut(s) | 301, 451 |
| BspPI | GGATC | 3 cut(s) | 48, 718, 731 |
| BspT107I | GGYRCC | 3 cut(s) | 436, 463, 662 |
| BspTNI | GGTCTC | 1 cut(s) | 173 |
| BsrBI | CCGCTC | 1 cut(s) | 224 |
| BsrFI | RCCGGY | 2 cut(s) | 115, 425 |
| BsrI | ACTGG | 1 cut(s) | 246 |
| BssAI | RCCGGY | 2 cut(s) | 115, 425 |
| BssECI | CCNNGG | 4 cut(s) | 63, 138, 139, 150 |
| BssMI | GATC | 3 cut(s) | 40, 723, 740 |
| BssNI | GRCGYC | 1 cut(s) | 663 |
| Bst2UI | CCWGG | 8 cut(s) | 140, 152, 189, 207, 212, 576, 700, 721 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 85 |
| BstACI | GRCGYC | 1 cut(s) | 663 |
| BstC8I | GCNNGC | 7 cut(s) | 36, 237, 389, 549, 586, 661, 732 |
| BstDEI | CTNAG | 4 cut(s) | 27, 125, 172, 297 |
| BstDSI | CCRYGG | 1 cut(s) | 63 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 254 |
| BstF5I | GGATG | 3 cut(s) | 58, 146, 513 |
| BstFNI | CGCG | 1 cut(s) | 397 |
| BstH2I | RGCGCY | 1 cut(s) | 666 |
| BstHHI | GCGC | 2 cut(s) | 382, 665 |
| BstKTI | GATC | 3 cut(s) | 43, 726, 743 |
| BstMAI | GTCTC | 2 cut(s) | 173, 586 |
| BstMBI | GATC | 3 cut(s) | 40, 723, 740 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 198, 207, 362, 377, 388, 466, 594, 634 |
| BstNI | CCWGG | 8 cut(s) | 140, 152, 189, 207, 212, 576, 700, 721 |
| BstNSI | RCATGY | 1 cut(s) | 752 |
| BstSCI | CCNGG | 9 cut(s) | 138, 150, 187, 205, 210, 574, 648, 698, 719 |
| BstSLI | GKGCMC | 2 cut(s) | 500, 586 |
| BstUI | CGCG | 1 cut(s) | 397 |
| BstV1I | GCAGC | 2 cut(s) | 204, 637 |
| BstX2I | RGATCY | 1 cut(s) | 723 |
| BstYI | RGATCY | 1 cut(s) | 723 |
| BsuRI | GGCC | 6 cut(s) | 210, 302, 317, 498, 584, 709 |
| BtgI | CCRYGG | 1 cut(s) | 63 |
| BtsCI | GGATG | 3 cut(s) | 58, 146, 513 |
| BtsIMutI | CAGTG | 1 cut(s) | 239 |
| BveI | ACCTGC | 2 cut(s) | 301, 451 |
| Cac8I | GCNNGC | 7 cut(s) | 36, 237, 389, 549, 586, 661, 732 |
| CfoI | GCGC | 2 cut(s) | 382, 665 |
| Cfr10I | RCCGGY | 2 cut(s) | 115, 425 |
| Cfr13I | GGNCC | 9 cut(s) | 154, 214, 247, 301, 496, 497, 582, 583, 707 |
| CseI | GACGC | 1 cut(s) | 603 |
| Csp6I | GTAC | 3 cut(s) | 437, 443, 464 |
| CviAII | CATG | 3 cut(s) | 130, 749, 784 |
| CviQI | GTAC | 3 cut(s) | 437, 443, 464 |
| DdeI | CTNAG | 4 cut(s) | 27, 125, 172, 297 |
| DinI | GGCGCC | 1 cut(s) | 664 |
| DpnI | GATC | 3 cut(s) | 42, 725, 742 |
| DpnII | GATC | 3 cut(s) | 40, 723, 740 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 797 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 797 |
| EaeI | YGGCCR | 1 cut(s) | 315 |
| EciI | GGCGGA | 3 cut(s) | 260, 444, 668 |
| Eco24I | GRGCYC | 4 cut(s) | 138, 146, 500, 586 |
| Eco31I | GGTCTC | 1 cut(s) | 173 |
| Eco47I | GGWCC | 3 cut(s) | 154, 214, 247 |
| Eco57I | CTGAAG | 1 cut(s) | 721 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 254 |
| EcoO109I | RGGNCCY | 3 cut(s) | 154, 214, 582 |
| EcoRII | CCWGG | 8 cut(s) | 138, 150, 187, 205, 210, 574, 698, 719 |
| EcoT38I | GRGCYC | 4 cut(s) | 138, 146, 500, 586 |
| EgeI | GGCGCC | 1 cut(s) | 664 |
| EheI | GGCGCC | 1 cut(s) | 664 |
| Esp3I | CGTCTC | 1 cut(s) | 586 |
| FaeI | CATG | 3 cut(s) | 133, 752, 787 |
| FaiI | YATR | 9 cut(s) | 131, 231, 266, 452, 623, 750, 785, 804, 806 |
| FaqI | GGGAC | 1 cut(s) | 140 |
| FatI | CATG | 3 cut(s) | 129, 748, 783 |
| FauI | CCCGC | 3 cut(s) | 165, 544, 593 |
| FauNDI | CATATG | 1 cut(s) | 804 |
| FblI | GTMKAC | 1 cut(s) | 415 |
| Fnu4HI | GCNGC | 2 cut(s) | 193, 626 |
| FokI | GGATG | 3 cut(s) | 45, 133, 500 |
| FriOI | GRGCYC | 4 cut(s) | 138, 146, 500, 586 |
| Fsp4HI | GCNGC | 2 cut(s) | 193, 626 |
| GlaI | GCGC | 2 cut(s) | 381, 664 |
| GluI | GCNGC | 2 cut(s) | 193, 626 |
| GsaI | CCCAGC | 1 cut(s) | 380 |
| GsuI | CTGGAG | 2 cut(s) | 558, 610 |
| HaeII | RGCGCY | 1 cut(s) | 666 |
| HaeIII | GGCC | 6 cut(s) | 210, 302, 317, 498, 584, 709 |
| HapII | CCGG | 3 cut(s) | 116, 426, 649 |
| HgaI | GACGC | 1 cut(s) | 603 |
| HhaI | GCGC | 2 cut(s) | 382, 665 |
| Hin1I | GRCGYC | 1 cut(s) | 663 |
| Hin1II | CATG | 3 cut(s) | 133, 752, 787 |
| Hin6I | GCGC | 2 cut(s) | 380, 663 |
| HinP1I | GCGC | 2 cut(s) | 380, 663 |
| HincII | GTYRAC | 4 cut(s) | 88, 94, 121, 416 |
| HindII | GTYRAC | 4 cut(s) | 88, 94, 121, 416 |
| HinfI | GANTC | 1 cut(s) | 569 |
| HpaI | GTTAAC | 2 cut(s) | 88, 94 |
| HpaII | CCGG | 3 cut(s) | 116, 426, 649 |
| HphI | GGTGA | 2 cut(s) | 211, 706 |
| Hpy166II | GTNNAC | 5 cut(s) | 88, 94, 121, 275, 416 |
| Hpy188I | TCNGA | 1 cut(s) | 740 |
| Hpy8I | GTNNAC | 5 cut(s) | 88, 94, 121, 275, 416 |
| HpyAV | CCTTC | 4 cut(s) | 260, 745, 805, 823 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 85 |
| HpyCH4IV | ACGT | 1 cut(s) | 90 |
| HpyCH4V | TGCA | 6 cut(s) | 285, 460, 614, 625, 730, 752 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 198, 207, 362, 377, 388, 466, 594, 634 |
| HpyF3I | CTNAG | 4 cut(s) | 27, 125, 172, 297 |
| HpySE526I | ACGT | 1 cut(s) | 90 |
| Hsp92I | GRCGYC | 1 cut(s) | 663 |
| Hsp92II | CATG | 3 cut(s) | 133, 752, 787 |
| HspAI | GCGC | 2 cut(s) | 380, 663 |
| KasI | GGCGCC | 1 cut(s) | 662 |
| KpnI | GGTACC | 2 cut(s) | 440, 467 |
| KspAI | GTTAAC | 2 cut(s) | 88, 94 |
| Kzo9I | GATC | 3 cut(s) | 40, 723, 740 |
| LmnI | GCTCC | 3 cut(s) | 31, 133, 399 |
| Lsp1109I | GCAGC | 2 cut(s) | 204, 637 |
| LweI | GCATC | 1 cut(s) | 447 |
| MaeII | ACGT | 1 cut(s) | 90 |
| MaeIII | GTNAC | 2 cut(s) | 768, 823 |
| MalI | GATC | 3 cut(s) | 42, 725, 742 |
| MbiI | CCGCTC | 1 cut(s) | 224 |
| MboI | GATC | 3 cut(s) | 40, 723, 740 |
| MboII | GAAGA | 1 cut(s) | 350 |
| MflI | RGATCY | 1 cut(s) | 723 |
| MhlI | GDGCHC | 6 cut(s) | 103, 138, 146, 404, 500, 586 |
| MlsI | TGGCCA | 1 cut(s) | 317 |
| MluCI | AATT | 4 cut(s) | 332, 555, 629, 675 |
| MluNI | TGGCCA | 1 cut(s) | 317 |
| Mly113I | GGCGCC | 1 cut(s) | 663 |
| MmeI | TCCRAC | 1 cut(s) | 339 |
| Mox20I | TGGCCA | 1 cut(s) | 317 |
| MscI | TGGCCA | 1 cut(s) | 317 |
| MseI | TTAA | 3 cut(s) | 75, 87, 93 |
| MslI | CAYNNNNRTG | 2 cut(s) | 323, 455 |
| Msp20I | TGGCCA | 1 cut(s) | 317 |
| MspA1I | CMGCKG | 5 cut(s) | 160, 176, 469, 566, 588 |
| MspI | CCGG | 3 cut(s) | 116, 426, 649 |
| MspR9I | CCNGG | 9 cut(s) | 140, 152, 189, 207, 212, 576, 650, 700, 721 |
| MvaI | CCWGG | 8 cut(s) | 140, 152, 189, 207, 212, 576, 700, 721 |
| MvnI | CGCG | 1 cut(s) | 397 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 198, 207, 362, 377, 388, 466, 594, 634 |
| NarI | GGCGCC | 1 cut(s) | 663 |
| NciI | CCSGG | 1 cut(s) | 650 |
| NdeI | CATATG | 1 cut(s) | 804 |
| NdeII | GATC | 3 cut(s) | 40, 723, 740 |
| NlaIII | CATG | 3 cut(s) | 133, 752, 787 |
| NlaIV | GGNNCC | 9 cut(s) | 135, 156, 438, 465, 498, 583, 584, 664, 725 |
| NmuCI | GTSAC | 1 cut(s) | 768 |
| NspI | RCATGY | 1 cut(s) | 752 |
| PaqCI | CACCTGC | 1 cut(s) | 301 |
| PasI | CCCWGGG | 1 cut(s) | 139 |
| PfeI | GAWTC | 1 cut(s) | 569 |
| PfoI | TCCNGGA | 2 cut(s) | 574, 698 |
| PkrI | GCNGC | 2 cut(s) | 194, 627 |
| PluTI | GGCGCC | 1 cut(s) | 666 |
| PpuMI | RGGWCCY | 2 cut(s) | 154, 214 |
| Psp1406I | AACGTT | 1 cut(s) | 90 |
| Psp5II | RGGWCCY | 2 cut(s) | 154, 214 |
| Psp6I | CCWGG | 8 cut(s) | 138, 150, 187, 205, 210, 574, 698, 719 |
| PspFI | CCCAGC | 1 cut(s) | 376 |
| PspGI | CCWGG | 8 cut(s) | 138, 150, 187, 205, 210, 574, 698, 719 |
| PspN4I | GGNNCC | 9 cut(s) | 135, 156, 438, 465, 498, 583, 584, 664, 725 |
| PspOMI | GGGCCC | 2 cut(s) | 496, 582 |
| PspPI | GGNCC | 9 cut(s) | 154, 214, 247, 301, 496, 497, 582, 583, 707 |
| PspPPI | RGGWCCY | 2 cut(s) | 154, 214 |
| PsuI | RGATCY | 1 cut(s) | 723 |
| RsaI | GTAC | 3 cut(s) | 438, 444, 465 |
| RsaNI | GTAC | 3 cut(s) | 437, 443, 464 |
| RseI | CAYNNNNRTG | 2 cut(s) | 323, 455 |
| SalI | GTCGAC | 1 cut(s) | 414 |
| SaqAI | TTAA | 3 cut(s) | 75, 87, 93 |
| SatI | GCNGC | 2 cut(s) | 193, 626 |
| Sau3AI | GATC | 3 cut(s) | 40, 723, 740 |
| Sau96I | GGNCC | 9 cut(s) | 154, 214, 247, 301, 496, 497, 582, 583, 707 |
| ScrFI | CCNGG | 9 cut(s) | 140, 152, 189, 207, 212, 576, 650, 700, 721 |
| SduI | GDGCHC | 6 cut(s) | 103, 138, 146, 404, 500, 586 |
| SfaNI | GCATC | 1 cut(s) | 447 |
| SfoI | GGCGCC | 1 cut(s) | 664 |
| SinI | GGWCC | 3 cut(s) | 154, 214, 247 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 323, 455 |
| Sse9I | AATT | 4 cut(s) | 332, 555, 629, 675 |
| SspDI | GGCGCC | 1 cut(s) | 662 |
| StyD4I | CCNGG | 9 cut(s) | 138, 150, 187, 205, 210, 574, 648, 698, 719 |
| TaaI | ACNGT | 2 cut(s) | 11, 85 |
| TaiI | ACGT | 1 cut(s) | 93 |
| TaqI | TCGA | 1 cut(s) | 415 |
| TasI | AATT | 4 cut(s) | 332, 555, 629, 675 |
| TfiI | GAWTC | 1 cut(s) | 569 |
| Tru1I | TTAA | 3 cut(s) | 75, 87, 93 |
| Tru9I | TTAA | 3 cut(s) | 75, 87, 93 |
| TscAI | CASTG | 1 cut(s) | 246 |
| TseFI | GTSAC | 1 cut(s) | 768 |
| TseI | GCWGC | 2 cut(s) | 192, 625 |
| Tsp45I | GTSAC | 1 cut(s) | 768 |
| TspRI | CASTG | 1 cut(s) | 246 |
| VpaK11BI | GGWCC | 3 cut(s) | 154, 214, 247 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 254 |
| XapI | RAATTY | 1 cut(s) | 332 |
| XceI | RCATGY | 1 cut(s) | 752 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 520 |
| XmiI | GTMKAC | 1 cut(s) | 415 |